Article
Chemistry, Analytical
Yi-Fan Tian, Yu-Peng Zhang, Qiu-Mei Wu, Dai-Wen Pang, Shu-Lin Liu, Zhi-Gang Wang
Summary: This article introduces a method for real-time dynamic translation imaging using the CRISPR/dCas13 system. The method labels the unmodified coding regions of target mRNA, enabling real-time visualization of the translation process, and is promising for further investigations into translation mechanisms.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yi-Fan Tian, Yu-Peng Zhang, Qiu-Mei Wu, Dai-Wen Pang, Shu-Lin Liu, Zhi-Gang Wang
Summary: Translation is a critical cellular activity regulated by viruses, and studying its kinetics and subcellular localization requires real-time visualization techniques. This study presents a new method using the CRISPR/dCas13 system and specific complementary guide RNAs to dynamically image translation without altering the coding regions. This tool provides promising opportunities for further investigating translation mechanisms.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Charlotte A. Cialek, Gabriel Galindo, Tatsuya Morisaki, Ning Zhao, Taiowa A. Montgomery, Timothy J. Stasevich
Summary: The authors developed live-cell biosensors to investigate the impact of Argonaute proteins on single-mRNA translation dynamics. They found that Argonaute association gradually silences translation and leads to the clustering and coalescence of mRNA with P-bodies, maintaining a translationally silent state.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Wanze Chen, Orane Guillaume-Gentil, Pernille Yde Rainer, Christoph G. Gaebelein, Wouter Saelens, Vincent Gardeux, Amanda Klaeger, Riccardo Dainese, Magda Zachara, Tomaso Zambelli, Julia A. Vorholt, Bart Deplancke
Summary: Live-seq is a novel single-cell transcriptomic profiling approach that preserves cell viability during RNA extraction, allowing for the correlation analysis between a cell's ground-state transcriptome and its downstream molecular or phenotypic behavior. It accurately stratifies diverse cell types and states without major cellular perturbations, and can be used to map a cell's trajectory and evaluate gene effects on cell phenotypes.
Article
Chemistry, Multidisciplinary
Rachel Wills, Jonathan Farhi, Patrick Czabala, Sophia Shahin, Jennifer M. Spangle, Monika Raj
Summary: Aliphatic aldehydes are reactive electrophilic carbonyls that cross-link with DNA and proteins leading to cellular toxicity and disease pathogenesis. Chemical sensors that generate a benzimidazole moiety upon reaction with aliphatic aldehydes of different chain lengths have been developed, enabling the real-time imaging and quantification of aldehyde formation in live mammalian cells. This tool has the potential to revolutionize aldehyde research by uncovering cellular metabolites that have previously remained elusive.
Article
Chemistry, Analytical
Jiseong Park, Yongdeok Ahn, Wonhee John Lee, Siwoo Jin, Sejoo Jeong, Jaeyong Kim, Young-Sam Lee, Jong-Chan Lee, Daeha Seo
Summary: Researchers have developed a method called lipid-MAP that uses nanoparticles, single-molecule localization microscopy, and a hidden Markov model algorithm to analyze the phase heterogeneity of lipid membranes. They were able to detect and characterize small and transient lipid domains in live cells.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jiseong Park, Yongdeok Ahn, Wonhee John Lee, Siwoo Jin, Sejoo Jeong, Jaeyong Kim, Young-Sam Lee, Jong-Chan Lee, Daeha Seo
Summary: This study presents a method for analyzing the heterogeneity of lipid membranes by detecting the instantaneous velocity change of single lipid molecules. The findings reveal the formation of small and transient lipid domains in live cells. Additionally, the study discusses the influence of membrane compositional changes on the formation of these domains.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jiseong Park, Yongdeok Ahn, Wonhee John Lee, Siwoo Jin, Sejoo Jeong, Jaeyong Kim, Young-Sam Lee, Jong-Chan Lee, Daeha Seo
Summary: The study presents a single lipid-molecular motion analysis pipeline (lipid-MAP) that utilizes the optical properties of nanoparticles, high spatial localization accuracy of single-molecule localization microscopy, and separation capability of the hidden Markov model algorithm to analyze the phase heterogeneity of lipid membranes in live cells. The findings show that individual lipid molecules are in dynamic equilibrium between two distinguishable phases, resulting in the formation of small, viscous, and transient domains. Furthermore, the study provides insights into how changes in membrane composition, such as cholesterol and phospholipids, affect domain formation.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ioannis Tsirkas, Daniel Dovrat, Manikandan Thangaraj, Ineke Brouwer, Amit Cohen, Zohar Paleiov, Michael M. Meijler, Tineke Lenstra, Amir Aharoni
Summary: The coexistence of DNA replication and transcription during S-phase requires tight coordination to prevent conflicts. This study developed a live-cell imaging approach to monitor the real-time progression of replisomes and transcription dynamics during a transcription-replication encounter. The findings showed a wave of partial transcriptional repression ahead of the moving replication fork, and revealed the negative impact of conflicts on both processes.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Nathan M. Livingston, Jiwoong Kwon, Oliver Valera, James A. Saba, Niladri K. Sinha, Pranav Reddy, Blake Nelson, Clara Wolfe, Taekjip Ha, Rachel Green, Jian Liu, Bin Wu
Summary: Stochasticity has been found to play a role in gene regulation, particularly in bursting transcription. However, the role of stochasticity in translation has not been fully explored due to limitations in imaging technology. This study developed techniques to track single mRNAs and their translation in live cells, revealing previously unknown dynamics of translation. The findings show that translation, like transcription, occurs in bursts of activity and inactivity, but with complex structures in the untranslated region affecting burst amplitudes. Bursting frequency can be controlled by specific sequences and trans-acting factors.
Article
Nanoscience & Nanotechnology
Mohit Rathor, Shivam Kumar Chaubey, Rupen Tamang, Biplob Koch, Rakesh Kumar Singh
Summary: This paper presents a label-free and single-shot noninvasive quantitative anisotropy imaging method for cancer cells using a polarization digital holographic microscope (PDHM). The PDHM's ability to tune the carrier frequency in recording and reconstructing polarization features from a single measurement offers a unique advantage for spatially resolved live imaging.
Article
Cell Biology
Igor Kukhtevich, Mariana Rivero-Romano, Namisha Rakesh, Poonam Bheda, Yagya Chadha, Paulina Rosales-Becerra, Stephan Hamperl, Daniela Bureik, Scarlett Dornauer, Catherine Dargemont, Antonis Kirmizis, Kurt M. Schmoller, Robert Schneider
Summary: RNA distribution during cell division is asymmetric and regulated by factors such as the lifespan regulator Sir2 and replicative age. This study establishes an approach to quantify RNA dynamics in single cells using the diSpinach aptamer in a microfluidics device and validates it using budding yeast as a model.
Article
Chemistry, Multidisciplinary
Shan Chen, Zhifei Xu, Shiwei Li, Hong Liang, Chen Zhang, Zonghua Wang, Jingying Li, Juan Li, Huanghao Yang
Summary: In this study, a cell membrane-anchored DNA multitasking processor was proposed for integrated analyses of cellular signal transduction. The membrane-anchored DNA processor effectively inhibited c-Met signaling and allowed monitoring of correlative VEGF secretion. The versatile aptameric nucleic acids used in this processor systematically dissected the coordination of cell surface receptors with related components.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Yi-Nan Liu, Zhen-Ting Lv, Wen-Li Lv, Dong-Feng Liu, Xian-Wei Liu
Summary: We developed a label-free and noninvasive imaging method to measure electron transfer in microbial cells. By imaging the redox reaction of cytochromes in microbial cells, we obtained the electrochemical activity parameters of individual cells, revealing heterogeneities in electron transfer at the single-cell level.
Article
Multidisciplinary Sciences
Linda S. Forero-Quintero, William Raymond, Tetsuya Handa, Matthew N. Saxton, Tatsuya Morisaki, Hiroshi Kimura, Edouard Bertrand, Brian Munsky, Timothy J. Stasevich
Summary: Through the combination of single-molecule microscopy and fluorescent antibody-based probes in living cells, the phosphorylation timing, kinetics, and spatial organization of RNAP2 on a single-copy gene were measured. Live-cell evidence showed heterogeneity in RNAP2 distribution along the gene length and clusters of Serine 5 phosphorylated RNAP2 separated from nascent mRNA synthesis in space and time.
NATURE COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)