Article
Chemistry, Multidisciplinary
Patrick D. Fischer, Abu Sayeed Chowdhury, Thomas Bartholow, Shibani Basu, Eric Baggs, Huel S. Cox, Srdan Matosin, Michael D. Burkart, Lisa Warner, Rajesh Nagarajan, Haribabu Arthanari
Summary: Acyl-homoserine lactone synthases produce specific AHL quorum sensing signals in Gram-negative bacteria to enhance virulence. This study demonstrates through solution NMR spectroscopy that the carrier protein-enzyme interface accurately reveals substrate recognition mechanisms in two quorum signal synthases.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Fuwen Liu, Fei Wang, Yixuan Yuan, Xiaoran Li, Xiaojun Zhong, Menghua Yang
Summary: Vibrio parahaemolyticus is a significant food-borne pathogen. The study showed that the three signal synthases, CqsA(vp), LuxM(vp), and LuxS(vp), are essential for activating quorum sensing and regulating swarming in V. parahaemolyticus. The absence of these synthases leads to swarming defects.
MOLECULAR MICROBIOLOGY
(2023)
Review
Environmental Sciences
Li Yi, Xiao Dong, Daniel Grenier, Kaicheng Wang, Yang Wang
Summary: The microbial community relies on quorum sensing as a communication tool to connect with the environment, involving the production and detection of extracellular signaling molecules. Different bacterial species possess structurally and functionally different quorum sensing receptors, contributing to the understanding of their specific functions in bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Engineering, Environmental
Chunxiao Dai, Yuanyuan Qu, Weize Wu, Shuzhen Li, Zhuo Chen, Shengyang Lian, Jiawei Jing
Summary: This study established a QS-related protein database and an automatic annotation pipeline for rapid and accurate annotation and classification of QS-related sequences in aquatic environments. It provides a useful tool for understanding bacterial communication in water environments.
Article
Environmental Sciences
Xiyuan Xu, Shuai Wang, Chengxuan Li, Jingxi Li, Fenglei Gao, Li Zheng
Summary: Microplastics in the oceans are a new type of pollutant with complex biofilms. This study investigated the diversity and biological characteristics of quorum sensing bacteria in microplastic-associated biofilms, and found that these bacteria have strong biofilm-forming capacity and potential to exacerbate adverse environmental effects.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Microbiology
Liushen Lu, Junwei Wang, Ting Qin, Kai Chen, Jun Xie, Bingwen Xi
Summary: In this study, it was found that carvacrol, a natural compound found in plants, can effectively inhibit bacterial communication and reduce the toxicity of pathogenic bacteria. Carvacrol was shown to reduce the production of violacein in Chromobacterium violaceum and the concentration of N-acyl-homoserine lactones (AHLs) in Aeromonas hydrophila NJ-35. These findings suggest that carvacrol could be a potential strategy for reducing the virulence of pathogenic bacteria.
Article
Environmental Sciences
M. de Celis, L. Serrano-Aguirre, I Belda, R. Liebana-Garcia, M. Arroyo, D. Marquina, I de la Mata, A. Santos
Summary: The study indicates that the use of AHLs-degrading enzymes can reduce biofilm formation on WWTP membranes and ameliorate the virulence of Gram-negative bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Microbiology
Lanlan Liu, Xiangyong Zeng, Jia Zheng, Yunman Zou, Shuyi Qiu, Yifeng Dai
Summary: This review explores the role of AHL-mediated quorum sensing (AHL-QS) in the regulation of bacterial substance and energy metabolism. The review provides insights into the typical molecular structures, general mechanism, and regulatory mechanisms of AHLs in various metabolic processes.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Engineering, Environmental
Jing Li, He Liu, Ping Wu, Peng Ding, Jie Zhang, Chao Zhang, Bo Fu, Min-hua Cui
Summary: This study investigates the enhancement of caproate production from ethanol and acetate in chain elongation enrichments by utilizing AHLs as signal molecules. The results show that adding C8-HSL significantly increases caproate concentration, possibly due to the increase in protein content and total biomass.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Engineering, Environmental
Xu Wang, Cancan Jiang, Danhua Wang, Yang Yang, Lijing Fan, Shengjun Xu, Xuliang Zhuang
Summary: Free nitrous acid (FNA) is widely used for wastewater management by altering sludge characteristic and function. This study investigated the role of collective behaviors in FNA treatment and revealed the impact of FNA on sludge morphology and composition.
Review
Microbiology
Rohit Patel, Mansi Soni, Bilv Soyantar, Suruchi Shivangi, Swati Sutariya, Meenu Saraf, Dweipayan Goswami
Summary: The indiscriminate use of antibiotics has led to the emergence of drug-resistant strains. Many infectious diseases are caused by microbes that can communicate through quorum sensing (QS), and interfering with QS can effectively control their pathogenicity. Inhibition of QS has become a promising approach for developing new drugs, and various quorum sensing inhibitors (QSIs) have been identified. This review provides an overview of the QS mechanism, QS inhibition, and compounds with anti-QS potential, as well as the possibility of quorum sensing resistance.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Environmental Sciences
Jie Hu, Jin Wang, Jiahao Li, Haidong Hu, Bin Wu, Hongqiang Ren, Jinfeng Wang
Summary: This study proposes a machine learning-based prediction model for AHLs synthases, called AHLS-pred. By comparing different feature extraction methods and machine learning algorithms, the final model achieves good performance on an independent testing dataset.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Engineering, Marine
Gary E. Schultz, Peter H. Santschi
Summary: Free-living bacteria in marine environments are believed to be too sparse to use density-dependent quorum sensing systems. However, this study found that marine bacteria do respond to signal molecules, indicating the presence of quorum sensing in marine gels. This discovery is significant for marine and environmental sciences.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Qiang Zhang, Sizhe Li, Maha Hachicha, Mohamed Boukraa, Laurent Soulere, Mohamed L. Efrit, Yves Queneau
Summary: This review summarizes the contribution of heterocyclic chemistry in the design of AHLs analogues, emphasizing how heterocyclic building blocks can serve as replacements of the lactone moiety, as a bioisostere for the amide group, or as an additional pattern appended to the side chain. Additionally, non-AHL-related heterocyclic compounds with AHL-like QS activity are also mentioned.
Article
Ecology
Mohammed Dwidar, Hyochan Jang, Naseer Sangwan, Wonsik Mun, Hansol Im, Sora Yoon, Sooin Choi, Dougu Nam, Robert J. Mitchell
Summary: The predatory bacterium Bdellovibrio bacteriovorus 109J preys on other Gram-negative bacteria to obtain nutrients for replication and survival. Two classes of bacterial signaling molecules, AHLs and DSF, were found to have different effects on the behavior and viability of B. bacteriovorus 109J. DSF significantly delayed predation and bdelloplast lysis, as well as reduced the motility of the cells. Additionally, DSF caused genome-wide changes in gene expression patterns, downregulating flagellum assembly genes and serine protease genes.
Article
Plant Sciences
Arata Yajima, Ryo Katsuta, Mikaho Shimura, Ayaka Yoshihara, Tatsuo Saito, Ken Ishigami, Kenji Kai
Summary: The study revisited the NMR data and proposed structures of bradyoxetin and HMCP, synthesized model compounds to validate the structural differences, and concluded that the proposed structures might be incorrect based on experimental results.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Kazuya Murata, Mayuna Suenaga, Kenji Kai
Summary: This study reports the isolation, structure elucidation, and biological characterization of a new class of bacterial polyynes called protegenins, which contribute to the antioomycete and plant-protective effects of Pseudomonas protegens.
ACS CHEMICAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Jota Maki, Hikari Nakatou, Kazumasa Tani, Eriko Eto, Kei Hayata, Dan Yamamoto, Kenji Kai, Takashi Tamada, Kazuyo Akamatsu, Kunihiro Kawanishi, Keiichiro Nakamura, Hisashi Masuyama
Summary: This RCT aims to investigate whether Spiral-thread sutures are superior to conventional sutures for preventing uterine scar thinning following elective cesarean section. The study will assess 200 women undergoing cesarean sections in Japan and compare scar thinning, operative time, blood loss, and other outcomes between the two suture types. The results are expected to provide valuable evidence on the optimal suture for preventing hysterotomy wound thinning.
CONTEMPORARY CLINICAL TRIALS
(2021)
Article
Biochemistry & Molecular Biology
Moeri Oshimo, Fumie Nakashima, Kenji Kai, Hirofumi Matsui, Takahiro Shibata, Mitsugu Akagawa
Summary: Sulfites, commonly used as preservatives and antioxidants in the food industry, have been shown to induce significant cytotoxicity in rat gastric mucosal cells through oxidative stress, forming protein carbonyls and 8-hydroxy-2'-deoxyguanosine. This indicates potential gastric tissue injury via formation of sulfite-related free radicals.
FREE RADICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Makoto Ihara, Keiji Tanaka, Kenji Kai, Hideo Hayashi, Kazuhiko Matsuda
Summary: The study has provided evidence that chrodrimanin B acts as a competitive antagonist on rat brain GABA(A) receptors, supporting previous observations on its action as a competitive antagonist on silkworm larval GABA receptors.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Yasuyuki Kobayashi, Natsumi Watanabe, Reina Hiura, Mai Kubota, Kousuke Furuta, Keiichiro Sugimoto, Kaeko Murota, Eri Nakamura, Toshiki Matsuura, Kenji Kai, Takashi Inui, Tomoya Kitakaze, Naoki Harada, Ryoichi Yamaji
Summary: This study provides insights into the transport form and pathway of oleamide, as well as its absorption and metabolism properties. Oleamide is primarily transported via the portal vein as a complex with albumin. It is taken up by CD36 in the small intestine and degraded intracellularly by FAAH.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Kanako Inoue, Chika Takemura, Wakana Senuma, Hidefumi Maeda, Kenji Kai, Akinori Kiba, Kouhei Ohnishi, Masayuki Tsuzuki, Yasufumi Hikichi
Summary: In this study, the infection route of Ralstonia solanacearum strain OE1-1 in tomato roots was investigated. The strain first attaches to the meristematic and elongation zones of tomato roots and then colonizes the intercellular spaces between the epidermis and cortex. It further invades the cortical cells and forms biofilms to progress through the cortex and endodermis, eventually infecting the pericycle cells and xylem vessels. The secretion of quorum sensing-inducible plant cell wall-degrading enzymes via the type II secretion system is essential for infection.
JOURNAL OF PLANT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Nao Matsukawa, Chiaki Tsumori, Kouhei Ohnishi, Kenji Kai
Summary: Strains of Ralstonia solanacearum species complex (RSSC) produce lipopeptides through the PKS-NRPS enzyme hybrid. Ralstonins have been found to be important in parasitism of RSSC to other hosts. This study discovered ralstopeptins A and B, cyclic lipopeptides with less amino acid residues than ralstonins, from strain MAFF 211519. The production of ralstopeptins was found to be dependent on the PKS-NRPS genes.
ACS CHEMICAL BIOLOGY
(2023)
Article
Plant Sciences
Wakana Senuma, Masayuki Tsuzuki, Chika Takemura, Yuki Terazawa, Kanako Inoue, Akinori Kiba, Kouhei Ohnishi, Kenji Kai, Yasufumi Hikichi
Summary: After infecting tomato plants, the bacterium Ralstonia pseudosolanacearum strain OE1-1 activates quorum sensing to induce the production of plant cell wall-degrading enzymes and invades xylem vessels to cause disease. A phcA-deletion mutant shows loss of virulence and inability to infect xylem vessels. A cbhA-deletion mutant also lacks the ability to infect xylem vessels and exhibits reduced virulence. Our study reveals that CbhA is involved in the full expression of phcA, contributing to the virulence of strain OE1-1.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Masayuki Tsuzuki, Kanako Inoue, Akinori Kiba, Kouhei Ohnishi, Kenji Kai, Yasufumi Hikichi
Summary: The Gram-negative bacterium Ralstonia pseudosolanacearum strain OE1-1 attaches to tomato roots, activates quorum sensing, and forms biofilms to infect xylem vessels. Quorum sensing involves the induction and activation of the transcriptional regulator PhcA, and is associated with the production of secondary metabolites and exopolysaccharides. These components contribute to the positive feedback loop of quorum sensing.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mana Kitao, Ai Yamaguchi, Takuma Tomioka, Kenji Kai, Yuki Kamei, Kenji Sugimoto, Mitsugu Akagawa
Summary: Age-related macular degeneration (AMD) is a disease that causes vision loss in the elderly. Blue light irradiation can induce cell death and oxidative stress in retinal pigment epithelium cells, but the antioxidant properties of astaxanthin can prevent this cell death. Therefore, astaxanthin may be a dietary strategy to prevent blue light-induced retinal degeneration, including AMD.
FREE RADICAL RESEARCH
(2023)
Article
Plant Sciences
Chika Takemura, Wakana Senuma, Masayuki Tsuzuki, Yuki Terazawa, Kanako Inoue, Masanao Sato, Akinori Kiba, Kouhei Ohnishi, Kenji Kai, Yasufumi Hikichi
Summary: Research shows that the protein ChpA regulates QS-dependent genes and affects the behavior in host plant roots and virulence of pathogenic bacterium Ralstonia pseudosolanacearum OE1-1.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Microbiology
Chiaki Tsumori, Shoma Matsuo, Yuta Murai, Kenji Kai
Summary: A study found that strains of Ralstonia solanacearum species complex (RSSC) invade the fungus Fusarium oxysporum by producing extracellular polysaccharide I (EPS I), biofilm, and the signal molecule ralstonins. This invasion process is regulated by the bacterial quorum sensing (QS) system, and is significant for the bacteria-fungus symbiosis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Organic
Mayuna Suenaga, Naoka Katayama, Kokoro Kitamura, Kenji Kai
Summary: Bacteria in the Pseudomonas protegens Cab57 strain have been found to produce bacterial polyynes, such as protegenin A, which can be converted into more complex polyynes, known as caryoynencins A-E, by introducing a cytochrome P450 gene from Burkholderia caryophylli. This study establishes a stable purification and structural determination method for various bacterial polyynes and highlights the potential of P. protegens Cab57 as an engineering host for producing biologically active polyynes.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Nutrition & Dietetics
Yasuyuki Kobayashi, Natsumi Watanabe, Tomoya Kitakaze, Keiichiro Sugimoto, Takeshi Izawa, Kenji Kai, Naoki Harada, Ryoichi Yamaji
Summary: The study showed that dietary oleamide can rescue skeletal muscle atrophy in mice, possibly by activating the PI3K/Akt/mTOR signaling pathway and restoring autophagy flux.
BRITISH JOURNAL OF NUTRITION
(2021)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
