Journal
CHEMICAL COMMUNICATIONS
Volume 49, Issue 20, Pages 2052-2054Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc00034f
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Funding
- NSFC [21275004, 20905062, 21075100, 21275119]
- New Century Excellent Talent Program of MOE
- Fundamental Research Funds for the Central Universities [XDJK2012A004]
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A highly sensitive electrochemical sequence-specific DNA detection strategy is demonstrated by coupling N.BstNB I (a nicking endonuclease)-assisted target recycling amplification with DNA supersandwich assembly signal enhancement. The proposed method avoids any extra chemical labeling steps and offers high selectivity against single-base mismatch sequences and a low detection limit down to 0.36 fM.
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