Journal
CHEMICAL COMMUNICATIONS
Volume 49, Issue 65, Pages 7219-7221Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc42485e
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Funding
- Natural Science Foundation of China [21176215, 21176102]
- Outstanding Young Scholar of Zhejiang Province [R4110092]
- Program for Zhejiang Leading Team of ST Innovation [2011R50007]
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A novel high-throughput screening method for directed co-evolution of endoglucanase and beta-glucosidase was constructed based on the synergy of the two enzymes using a bicistronic operon. To our knowledge, this is the first report of directed co-evolution of two cellulosic enzymes using an insoluble screening substrate via their synergistic reactions.
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