Review
Pharmacology & Pharmacy
Margaret Wallen, Farrukh Aqil, Wendy Spencer, Ramesh C. Gupta
Summary: Despite its introduction more than three decades ago, gene therapy has not lived up to its potential due to the lack of an effective delivery system. This review highlights the use of exosomes derived from bovine milk and colostrum as a nano-delivery platform for gene therapy. These exosomes provide an abundant, scalable, and cost-effective source for targeted delivery of nucleic acids, overcoming the limitations of current gene therapy delivery techniques.
Article
Engineering, Chemical
P. Ferreira, M. Riscado, S. Bernardo, M. G. Freire, J. L. Faria, APM. Tavares, CG. Silva, F. Sousa
Summary: Therapeutic approaches based on nucleic acids have gained attention, but require effective manufacturing strategies and precise quality control. This study describes a simple and efficient method using carbon nanotubes to selectively capture RNA and other impurities, resulting in the isolation and clarification of plasmid DNA (pDNA) from complex samples.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Yuta Hagino, Ikramy A. Khalil, Seigo Kimura, Kenji Kusumoto, Hideyoshi Harashima
Summary: This study developed lipid nanoparticles (LNPs) for targeted delivery of plasmid DNA (pDNA) to the lungs using the GALA peptide. The double-coated LNPs significantly improved transfection activity in the lungs, achieving high gene expression levels and lung-selective delivery. GALA/R8 modification and the double-coating design were essential for efficient gene expression in the lungs.
MOLECULAR PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
C. Bellefroid, C. Reusch, A. Lechanteur, B. Evrard, F. Debacq-Chainiaux, D. Mottet, G. Piel
Summary: This study aimed to develop a suitable gene delivery formulation for topical use on skin, and found that different lipid combinations can effectively deliver plasmid DNA into skin cells. Certain formulations showed high transfection rates with limited cytotoxicity, presenting promising potential for gene therapy in treating skin diseases.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Oncology
Radha Munagala, Farrukh Aqil, Jeyaprakash Jeyabalan, Raghuram Kandimalla, Margaret Wallen, Neha Tyagi, Sarah Wilcher, Jun Yan, David J. Schultz, Wendy Spencer, Ramesh C. Gupta
Summary: The use of bovine colostrum exosomes and polyethyleneimine matrix for gene delivery shows promise in targeting KRAS and WT p53 for effective gene therapy, representing a novel platform for therapeutic nucleic acids delivery to treat human disease.
Article
Biochemical Research Methods
Wenjing Huang, Shinya Sakuma, Naotomo Tottori, Shigeo S. Sugano, Yoko Yamanishi
Summary: This study demonstrates a technology of electromechanical poration for transfecting large molecules into difficult-to-inject cells, with the potential to enhance transfection efficiency by adjusting the viscosity of the cell suspension.
Article
Chemistry, Multidisciplinary
Seigo Kimura, Hideyoshi Harashima
Summary: The era of nucleic acid nanomedicine has arrived, as evidenced by the successful use of LNPs in delivering nucleic acid molecules. Further research is needed to target tissues other than the liver and improve gene expression efficiency.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Da Sun, Wenyu Sun, Song-Qi Gao, Cheng Wei, Amirreza Naderi, Andrew L. Schilb, Josef Scheidt, Sangjoon Lee, Timothy S. Kern, Krzysztof Palczewski, Zheng-Rong Lu
Summary: Developing gene replacement therapy for large gene mutations in retinal disorders, such as Stargardt disease, remains a challenge. A non-viral gene therapy using ECO/pDNA nanoparticles has shown promise, with modified ABCA4 plasmids with SV40 enhancer demonstrating increased gene expression. The addition of sucrose stabilized the nanoparticles, leading to improved transfection efficiency, while sorbitol showed fluctuating results.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Wantao Tang, Ting Tong, Hong Wang, Xuehe Lu, Changping Yang, Yushuai Wu, Yuang Wang, Jianbing Liu, Baoquan Ding
Summary: DNA nanostructures have been widely used in drug delivery systems. This study presents a DNA origami-based CRISPR/Cas9 gene editing system for efficient gene therapy in vivo. The designed DNA origami structure can recruit and load the sgRNA/Cas9 complex for targeted gene editing, and achieve effective drug delivery.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Pharmacology & Pharmacy
Hardy Mitdank, Simko Sama, Meike Troeger, Maria Francesca Testa, Mattia Ferrarese, Dario Balestra, Mirko Pinotti, Alexander Weng
Summary: Minicircle DNA is a promising tool in gene therapy with advantages such as higher transfection efficiency and longer expression time, but its cost-intensive production limits broader use. An optimized production method can lead to high-quality minicircle DNA, which has been investigated for pharmaceutical potential in vitro in a tumor cell model.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Aleksandra Cvetkoska, Janja Dermol-Cerne, Damijan Miklavcic, Simona Kranjc Brezar, Bostjan Markelc, Gregor Sersa, Matej Rebersek
Summary: In this study, a new pulse generator, applicator, and pulse delivery protocol for gene electrotransfer (GET) to skin cells were developed. The results showed that the proposed pulse delivery protocol resulted in higher gene expression in mouse skin cells compared to the currently used pulse generator, applicator, and pulse delivery protocol.
Article
Nanoscience & Nanotechnology
Yu-Hang Wan, Meng-Lei Huan, Chong-Xiao Yun, Tian-Yan Han, Si-Yuan Zhou, Xiao Song, Bang-Le Zhang
Summary: Acute lung injury (ALI) can be caused by various injury factors and is closely related to inflammatory reaction and cellular ferroptosis. GPX4 plays a crucial role in the inflammatory reaction and is the core regulatory protein of ferroptosis. The use of mPEI/pGPX4 nanoparticles, based on mannitol-modified polyethyleneimine (mPEI), could up-regulate the gene expression of GPX4, inhibit inflammatory reaction and cellular ferroptosis, effectively alleviating ALI in vitro and in vivo. This study suggests that gene therapy with pGPX4 holds potential as a therapeutic system for ALI treatment.
Article
Biochemistry & Molecular Biology
Xi Wang, Ling Zhao, Xiaoxing Wu, Huaxiu Luo, Di Wu, Meng Zhang, Jing Zhang, Mikhail Pakvasa, William Wagstaff, Fang He, Yukun Mao, Yongtao Zhang, Changchun Niu, Meng Wu, Xia Zhao, Hao Wang, Linjuan Huang, Deyao Shi, Qing Liu, Na Ni, Kai Fu, Kelly Hynes, Jason Strelzow, Mostafa El Dafrawy, Tong-Chuan He, Hongbo Qi, Zongyue Zeng
Summary: Isolation of plasmid DNA (pDNA) from bacterial cells is a critical step in molecular cloning and biomedical research. The use of size selection magnetic beads (SSMBs) in RNA depletion has been shown to be a cost-effective and efficient method for improving the quality and integrity of pDNA samples. The SSMB method can completely deplete contaminating RNA in both small-scale and large-scale pDNA preparations, making it a valuable tool for routine pDNA purification.
Article
Chemistry, Physical
Mehri Karimi Jabali, Ali Reza Allafchian, Seyed Amir Hossein Jalali, Hamideh Shakeripour, Rezvan Mohammadinezhad, Fahime Rahmani
Summary: The study introduces the use of ascorbic acid-modified chitosan as a pDNA nanocarrier, demonstrating promising pDNA release in intestinal fluids and potential biocompatibility with cell lines Vero and EPC. Chitosan effectively shields pDNA in gastric environments and allows for release in alkaline intestinal tissues, presenting a novel approach for gene delivery through oral administration.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Biology
Chenhao Li, Longfei Wang, Leland J. J. Cseke, Fernanda Vasconcelos, Jose Carlos Huguet-Tapia, Walter Gassmann, Laurens Pauwels, Frank F. F. White, Hansong Dong, Bing Yang
Summary: A versatile cytosine base editor system has been developed that can efficiently convert C to T in the genome of phytopathogenic bacteria, including Xanthomonas, Pseudomonas, Erwinia, and Agrobacterium. CRISPR-based genome editing variants have limitations due to unique host mechanisms, but the developed CRISPR-dCas9 and nCas9 deaminase vectors overcome these limitations. Using different promoters, the system achieved nearly 100% modification of the target region and successfully created base edits in multiple genes. Further modifications reduced off-target effects, improving the editing tool for a broad group of phytopathogenic bacteria.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Analytical
Xinyi Luo, Yingying Xue, Enguo Ju, Yu Tao, Mingqiang Li, Li Zhou, Chongguang Yang, Jianhua Zhou, Jiasi Wang
Summary: Early and accurate diagnosis of viruses is critical for control of the pandemic. In this study, a new gene RNA detection platform was developed for quantitative detection of viral RNA with high sensitivity and specificity.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Shishi Shen, Xi Cheng, Luyao Zhou, Yipeng Zhao, Hai Wang, Jie Zhang, Xiaobo Sun, Yuge Wang, Yaqing Shu, Yanteng Xu, Yu Tao, Mingqiang Li, Zhengqi Lu, Wei Cai, Guangjun Nie, Wei Qiu
Summary: This study developed neutrophil-derived nanovesicles (NNVs) to enhance the efficiency of myelin debris clearance in microglia for multiple sclerosis (MS) therapy. The results showed that NNVs treatment ameliorated lesional neuroinflammation and improved neurological functions and white matter integrity in a mouse model of MS. Additionally, NNVs treatment upregulated the expression of NRF2 in microglia and activated the transcription of RUBCN, leading to enhanced phagocytosis of myelin debris.
Article
Nanoscience & Nanotechnology
Wentao Dang, Yuqin Wang, Wei-Chih Chen, Enguo Ju, Rachel L. Mintz, Yue Teng, Lili Zhu, Kun Wang, Shixian Lv, Hon Fai Chan, Yu Tao, Mingqiang Li
Summary: A 3D printed hydrogel scaffold is developed for sustained release of drugs, enabling postoperative synergistic cancer therapy. The therapeutic efficiency can be adjusted by altering the amount of drugs and copper ions. The combination of chemotherapy and chemodynamic therapy induces apoptosis and ferroptosis of cancer cells. Results show that the hydrogel scaffold effectively inhibits tumor growth, offering a new strategy for preventing postoperative cancer recurrence.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Chenya Zhuo, Huiming Kong, Ke Yi, Chun-Wei Chi, Jiabing Zhang, Ran Chen, Haixia Wang, Caixia Wu, Yeh-Hsing Lao, Yu Tao, Mingqiang Li
Summary: A liver-specific CRISPR/Cas9 magnetic nanosystem is developed to treat chronic hepatitis B infection. With the help of magnetic field, the system enables spatially specific distribution and hepatocyte-specific gene editing, resulting in effective inactivation of HBV genome without off-target effects.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Chunxiong Zheng, Qingguo Zhong, Wantong Song, Ke Yi, Huimin Kong, Haixia Wang, Yu Tao, Mingqiang Li, Xuesi Chen
Summary: A core-shell membrane-fusogenic liposome (MFL) was designed to reverse the immunosuppressive effects of tumor cell surface glycans and enhance tumor elimination through the presentation of NK-activating glycans. This was achieved by fusing the MFL with tumor cell membranes, delivering sialyltransferase-inhibitor-loaded cores into the cytoplasm to downregulate immunosuppressive sialic acid, and anchoring NK-activating-glycan-modified shells onto the tumor surface. The reprogrammed tumor surface resulted in NK activation and improved tumor susceptibility to NK-cell-mediated recognition and lysis.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Yongwei Hu, Yanteng Xu, Rachel L. Mintz, Xing Luo, Youqiang Fang, Yeh-Hsing Lao, Hon Fai Chan, Kai Li, Shixian Lv, Guojun Chen, Yu Tao, Yun Luo, Mingqiang Li
Summary: A self-intensified in-situ therapy approach using electrospun fibers loaded with a biomimetic nanozyme and doxorubicin (DOX) has been developed to prevent tumor recurrence and metastasis after surgery.
Article
Engineering, Biomedical
Shuai Deng, Xiaoyu Zhao, Yanlun Zhu, Ning Tang, Rongliang Wang, Xuerao Zhang, Fuyang Qu, Yi-Ping Ho, Wayne Yuk-Wai Lee, Jiansu Chen, Mingqiang Li, Yu Tao, Hon Fai Chan
Summary: Liver tissue engineering is advanced through the fabrication of collagen type I microspheres, enabling efficient hepatic differentiation of stem cells and assembly of prevascularized liver tissue. The microspheres exhibit high uniformity and can self-assemble with endothelial cells to form high cell-packing density liver tissue. This technology has potential applications in regenerative medicine, drug screening, and in vitro liver modeling.
Article
Engineering, Biomedical
Jiabin Zhang, Yanteng Xu, Chenya Zhuo, Run Shi, Haixia Wang, Zuquan Hu, Hon Fai Chan, Hae-Won Kim, Yu Tao, Mingqiang Li
Summary: Engineering hepatocytes as multicellular cell spheroids can improve their viability for rescuing acute liver failure. This study developed a magnetic system for generating functional hepatocyte spheroids, which showed superior hepatic functions and therapeutic effects in vivo. The magnetic system holds great potential for future clinical translation.
Article
Chemistry, Multidisciplinary
Youqiang Fang, Xing Luo, Yanteng Xu, Zheng Liu, Rachel L. Mintz, Haiyang Yu, Xuan Yu, Kai Li, Enguo Ju, Haixia Wang, Zhaohui Tang, Yu Tao, Mingqiang Li
Summary: An implantable sandwich-structured dual-drug depot is developed to sequentially trigger a self-intensified starvation therapy and hypoxia-induced chemotherapy. The outer layers are 3D-printed with a calcium-crosslinked mixture ink, while the inner layer is a patch of electrospun fibers loaded with TPZ. The released drugs destroy blood vessels, inhibit angiogenesis, and induce cytotoxicity, effectively inhibiting tumor recurrence and metastasis.
Article
Microbiology
Jungang Lan, Yeqing Wang, Shusheng Yue, Duo Xu, Yinan Li, Xiangyu Peng, Jiao Hu, Enguo Ju, Shanping He, Tingting Li
Summary: KSHV causes both latent and lytic infections, and PEL is a B-cell lymphoma that is predominantly latently infected by KSHV. Inhibiting FoxO proteins can induce KSHV lytic replication and result in massive cell death in PEL.
Article
Multidisciplinary Sciences
Chunxiong Zheng, Qingguo Zhong, Ke Yi, Huimin Kong, Fangfang Cao, Chenya Zhuo, Yanteng Xu, Run Shi, Enguo Ju, Wantong Song, Yu Tao, Xiaoyuan Chen, Mingqiang Li
Summary: Equipping multiple functionalities on adoptive effector cells is essential for superior antitumor efficacy in solid tumors. However, current cell engineering technologies cannot achieve this in a single step due to differences in the spatial distributions of targets within a cell. In this study, a core-shell anti-phagocytosis-blocking repolarization-resistant membrane-fusogenic liposome (ARMFUL) is proposed to achieve one-step multiplexing cell engineering. This allows for the customization of multimodal cellular functions, enhancing adoptive cell therapy.
Article
Engineering, Biomedical
Hongyan Wei, Fenfang Li, Tiantian Xue, Haixia Wang, Enguo Ju, Mingqiang Li, Yu Tao
Summary: This study demonstrated that the tetrahedral DNA (TDN) nanoplatform efficiently delivered liver-specific miR122 to human mesenchymal stem cells (hMSCs), inducing their differentiation into functional hepatocyte-like cells (HLCs), suggesting a new approach for cell-based therapy in acute liver failure.
BIOACTIVE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Enguo Ju, Mengran Peng, Yanteng Xu, Yuqin Wang, Feng Zhou, Haixia Wang, Mingqiang Li, Yue Zheng, Yu Tao
Summary: In this study, a microneedle patch loaded with MnO2/Cu2O nanosheets and combretastatin A4 was designed for the enhanced therapy of cutaneous squamous cell carcinoma (cSCC). The patch effectively delivered drugs locally to the tumor sites and the MnO2/Cu2O exhibited both photothermal conversion and glucose oxidase (GOx)-mimicking activity. This work opens up new avenues for constructing multimodal treatment for efficient therapy of skin cancers.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Nanoscience & Nanotechnology
Fenfang Li, Jiabin Zhang, Ke Yi, Haixia Wang, Hongyan Wei, Hon Fai Chan, Yu Tao, Mingqiang Li
Summary: This review provides an overview of the current research on stem cell secretome, focusing on its preparation and delivery methods, as well as its therapeutic mechanisms. Future opportunities and challenges in the field of stem cell secretome therapy are also discussed.
ACS APPLIED BIO MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
