Imaging beyond the proteome

3D Imaging by Mass Spectrometry: A New Frontier

(2012) Erin H. Seeley et al.   ANALYTICAL CHEMISTRY

Glycan-Specific Metabolic Oligosaccharide Engineering of C7-Substituted Sialic Acids

(2012) Heinz Möller et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling

(2012) Chayasith Uttamapinant et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Thiacycloalkynes for Copper-Free Click Chemistry

(2012) Gabriela de Almeida et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Genetically Encoded Norbornene Amino Acid for the Mild and Selective Modification of Proteins in a Copper-Free Click Reaction

(2012) Emine Kaya et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Click-Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core

(2012) Audrey Dumont et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Imaging the Sialome during Zebrafish Development with Copper-Free Click Chemistry

(2012) Karen W. Dehnert et al.   CHEMBIOCHEM

Polar Dibenzocyclooctynes for Selective Labeling of Extracellular Glycoconjugates of Living Cells

(2012) Frédéric Friscourt et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reactivity of Biarylazacyclooctynones in Copper-Free Click Chemistry

(2012) Chelsea G. Gordon et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Multi-isotope imaging mass spectrometry quantifies stem cell division and metabolism

(2012) Matthew L. Steinhauser et al.   NATURE

Near-infrared fluorescent sensor for in vivo copper imaging in a murine Wilson disease model

(2012) T. Hirayama et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bioconjugation with Strained Alkenes and Alkynes

(2011) Marjoke F. Debets et al.   ACCOUNTS OF CHEMICAL RESEARCH

From Mechanism to Mouse: A Tale of Two Bioorthogonal Reactions

(2011) Ellen M. Sletten et al.   ACCOUNTS OF CHEMICAL RESEARCH

Bioorthogonal Chemical Reporters for Analyzing Protein Lipidation and Lipid Trafficking

(2011) Howard C. Hang et al.   ACCOUNTS OF CHEMICAL RESEARCH

Biomedical Applications of Tetrazine Cycloadditions

(2011) Neal K. Devaraj et al.   ACCOUNTS OF CHEMICAL RESEARCH

Photoinducible Bioorthogonal Chemistry: A Spatiotemporally Controllable Tool to Visualize and Perturb Proteins in Live Cells

(2011) Reyna K. V. Lim et al.   ACCOUNTS OF CHEMICAL RESEARCH

Metabolic Labeling of Fucosylated Glycans in Developing Zebrafish

(2011) Karen W. Dehnert et al.   ACS Chemical Biology

Tracking N-Acetyllactosamine on Cell-Surface Glycans In Vivo

(2011) Tianqing Zheng et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Increasing the Efficacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study

(2011) Christen Besanceney-Webler et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy

(2011) Wei Min et al.   Annual Review of Physical Chemistry

Classifying Human Brain Tumors by Lipid Imaging with Mass Spectrometry

(2011) L. S. Eberlin et al.   CANCER RESEARCH

Intracellular Detection of Cytosine Incorporation in Genomic DNA by Using 5-Ethynyl-2′-Deoxycytidine

(2011) Lirui Guan et al.   CHEMBIOCHEM

Bioorthogonal chemical tagging of protein cholesterylation in living cells

(2011) William P. Heal et al.   CHEMICAL COMMUNICATIONS

Exploring Protein Lipidation with Chemical Biology

(2011) Howard C. Hang et al.   CHEMICAL REVIEWS

MALDI Imaging of Lipid Biochemistry in Tissues by Mass Spectrometry

(2011) Karin A. Zemski Berry et al.   CHEMICAL REVIEWS

A Nuclear-Localized Fluorescent Hydrogen Peroxide Probe for Monitoring Sirtuin-Mediated Oxidative Stress Responses In Vivo

(2011) Bryan C. Dickinson et al.   CHEMISTRY & BIOLOGY

Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy

(2011) Andrew Folick et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Imaging sialylated tumor cell glycans in vivo

(2011) André A. Neves et al.   FASEB JOURNAL

A Targetable Fluorescent Sensor Reveals That Copper-DeficientSCO1andSCO2Patient Cells Prioritize Mitochondrial Copper Homeostasis

(2011) Sheel C. Dodani et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Bioorthogonal Quadricyclane Ligation

(2011) Ellen M. Sletten et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Designed To Dissolve: Suppression of Colloidal Aggregation of Cu(I)-Selective Fluorescent Probes in Aqueous Buffer and In-Gel Detection of a Metallochaperone

(2011) M. Thomas Morgan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemical contrast for imaging living systems: molecular vibrations drive CARS microscopy

(2011) John Paul Pezacki et al.   Nature Chemical Biology

Highly specific label-free molecular imaging with spectrally tailored excitation-stimulated Raman scattering (STE-SRS) microscopy

(2011) Christian W. Freudiger et al.   Nature Photonics

Imaging mass spectrometry in microbiology

(2011) Jeramie D. Watrous et al.   NATURE REVIEWS MICROBIOLOGY

Synthesis of a Fluorogenic Cyclooctyne Activated by Cu-Free Click Chemistry

(2011) John C. Jewett et al.   ORGANIC LETTERS

Calcium-dependent copper redistributions in neuronal cells revealed by a fluorescent copper sensor and X-ray fluorescence microscopy

(2011) S. C. Dodani et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic metabolic labeling of DNA in vivo with arabinosyl nucleosides

(2011) A. B. Neef et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Metabolic cross-talk allows labeling of O-linked  -N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway

(2011) M. Boyce et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An Expanded Palette of Genetically Encoded Ca2+ Indicators

(2011) Y. Zhao et al.   SCIENCE

Development and evaluation of new cyclooctynes for cell surface glycan imaging in cancer cells

(2011) Henning Stöckmann et al.   Chemical Science

Readily Accessible Bicyclononynes for Bioorthogonal Labeling and Three-Dimensional Imaging of Living Cells

(2010) Jan Dommerholt et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Labeling Live Cells by Copper-Catalyzed Alkyne−Azide Click Chemistry

(2010) Vu Hong et al.   BIOCONJUGATE CHEMISTRY

A Novel Imaging Approach for Early Detection of Prostate Cancer Based on Endogenous Zinc Sensing

(2010) S. K. Ghosh et al.   CANCER RESEARCH

Rapid Multilabel Detection of Geranylgeranylated Proteins by Using Bioorthogonal Ligation Chemistry

(2010) Alexandra F. H. Berry et al.   CHEMBIOCHEM

Evaluation of Alkyne-Modified Isoprenoids as Chemical Reporters of Protein Prenylation

(2010) Amanda J. DeGraw et al.   Chemical Biology & Drug Design

Mass Spectrometric Imaging for Biomedical Tissue Analysis

(2010) Kamila Chughtai et al.   CHEMICAL REVIEWS

Visualization of DNA methylation and histone modifications in living cells

(2010) Hiroshi Kimura et al.   CURRENT OPINION IN CELL BIOLOGY

A Palette of Fluorescent Probes with Varying Emission Colors for Imaging Hydrogen Peroxide Signaling in Living Cells

(2010) Bryan C. Dickinson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Real-Time Bioluminescence Imaging of Glycans on Live Cells

(2010) Allison S. Cohen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biocompatible Copper(I) Catalysts for in Vivo Imaging of Glycans

(2010) David Soriano del Amo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rapid Cu-Free Click Chemistry with Readily Synthesized Biarylazacyclooctynones

(2010) John C. Jewett et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Organelle-Targetable Fluorescent Probes for Imaging Hydrogen Peroxide in Living Cells via SNAP-Tag Protein Labeling

(2010) Duangkhae Srikun et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Visualizing Ascorbate-Triggered Release of Labile Copper within Living Cells using a Ratiometric Fluorescent Sensor

(2010) Dylan W. Domaille et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Alkynyl-farnesol reporters for detection ofproteinS-prenylation in cells

(2010) Guillaume Charron et al.   Molecular BioSystems

Nox2 redox signaling maintains essential cell populations in the brain

(2010) Bryan C Dickinson et al.   Nature Chemical Biology

The chemical toolbox for monitoring protein fatty acylation and prenylation

(2010) Rami N Hannoush et al.   Nature Chemical Biology

Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano

(2010) Kazuki Horikawa et al.   NATURE METHODS

Aquaporin-3 mediates hydrogen peroxide uptake to regulate downstream intracellular signaling

(2010) E. W. Miller et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Visualizing enveloping layer glycans during zebrafish early embryogenesis

(2010) J. M. Baskin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Copper-free click chemistry in living animals

(2010) P. V. Chang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering

(2010) B. G. Saar et al.   SCIENCE

Imaging the Lipidome: ω-Alkynyl Fatty Acids for Detection and Cellular Visualization of Lipid-Modified Proteins

(2009) Rami N. Hannoush et al.   ACS Chemical Biology

In VivoImaging ofCaenorhabditis elegansGlycans

(2009) Scott T. Laughlin et al.   ACS Chemical Biology

Cellular imaging with secondary ion mass spectrometry

(2009) John S. Fletcher   ANALYST

Metabolic Labeling of Sialic Acids in Living Animals with Alkynyl Sugars

(2009) Pamela V. Chang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Constructing and Exploiting the Fluorescent Protein Paintbox (Nobel Lecture)

(2009) Roger Y. Tsien   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Selective Fluorescence Labeling of Lipids in Living Cells

(2009) Anne B. Neef et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

(2009) Ellen M. Sletten et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Chemical Biology of Protein Phosphorylation

(2009) Mary Katherine Tarrant et al.   Annual Review of Biochemistry

Fluorescent Probes for Live-Cell RNA Detection

(2009) Gang Bao et al.   Annual Review of Biomedical Engineering

Aza-dibenzocyclooctynes for fast and efficient enzyme PEGylation via copper-free (3+2) cycloaddition

(2009) Marjoke F. Debets et al.   CHEMICAL COMMUNICATIONS

MALDI Mass Spectrometry Imaging, from its Origins up to Today: The State of the Art

(2009) Simona Francese et al.   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Fluorescent biosensors for real-time tracking of post-translational modification dynamics

(2009) Nwe-Nwe Aye-Han et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Chemical tools for understanding protein lipidation in eukaryotes

(2009) Guillaume Charron et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Fluorescent protein-based biosensors: resolving spatiotemporal dynamics of signaling

(2009) Lisa M DiPilato et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Metabolic glycoengineering: Sialic acid and beyond

(2009) Jian Du et al.   GLYCOBIOLOGY

Label-free quantitative analysis of lipid metabolism in livingCaenorhabditis elegans

(2009) Thuc T. Le et al.   JOURNAL OF LIPID RESEARCH

Rapid and selective detection of fatty acylated proteins using ω-alkynyl-fatty acids and click chemistry

(2009) Megan C. Yap et al.   JOURNAL OF LIPID RESEARCH

Copper-Responsive Magnetic Resonance Imaging Contrast Agents

(2009) Emily L. Que et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reactivity and Regioselectivity in 1,3-Dipolar Cycloadditions of Azides to Strained Alkynes and Alkenes: A Computational Study

(2009) Franziska Schoenebeck et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Simple and Effective Strategy To Increase the Sensitivity of Fluorescence Probes in Living Cells

(2009) Saki Izumi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Selective Labeling of Living Cells by a Photo-Triggered Click Reaction

(2009) Andrei A. Poloukhtine et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Imaging chromophores with undetectable fluorescence by stimulated emission microscopy

(2009) Wei Min et al.   NATURE

A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish

(2009) Philipp Niethammer et al.   NATURE

Translating metabolic exchange with imaging mass spectrometry

(2009) Yu-Liang Yang et al.   Nature Chemical Biology

A genetically encoded reporter of synaptic activity in vivo

(2009) Elena Dreosti et al.   NATURE METHODS

High-efficiency labeling of sialylated glycoproteins on living cells

(2009) Ying Zeng et al.   NATURE METHODS

Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

(2009) Lin Tian et al.   NATURE METHODS

Metabolic labeling and direct imaging of choline phospholipids in vivo

(2009) C. Y. Jao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Real-time imaging of histone H4 hyperacetylation in living cells

(2009) K. Sasaki et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Deep-Sea Archaea Fix and Share Nitrogen in Methane-Consuming Microbial Consortia

(2009) A. E. Dekas et al.   SCIENCE

Controlled Nucleation of DNA Metallization

(2008) Christian T. Wirges et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A FRET-Based Fluorogenic Phosphine for Live-Cell Imaging with the Staudinger Ligation

(2008) Matthew J. Hangauer et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Visualizing Metabolically Labeled Glycoconjugates of Living Cells by Copper-Free and Fast Huisgen Cycloadditions

(2008) Xinghai Ning et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Postsynthetic DNA Modification through the Copper-Catalyzed Azide-Alkyne Cycloaddition Reaction

(2008) Philipp M. E. Gramlich et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine

(2008) Conor L. Evans et al.   Annual Review of Analytical Chemistry

Advances in Imaging Secondary Ion Mass Spectrometry for Biological Samples

(2008) Steven G. Boxer et al.   Annual Review of Biophysics

Metals in Neurobiology: Probing Their Chemistry and Biology with Molecular Imaging

(2008) Emily L. Que et al.   CHEMICAL REVIEWS

A Targetable Fluorescent Probe for Imaging Hydrogen Peroxide in the Mitochondria of Living Cells

(2008) Bryan C. Dickinson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

New Strategy for Quantifying Biological Zinc by a Modified Zinpyr Fluorescence Sensor

(2008) Xiao-an Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Tetrazine Ligation: Fast Bioconjugation Based on Inverse-Electron-Demand Diels−Alder Reactivity

(2008) Melissa L. Blackman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Measuring calcium dynamics in living cells with genetically encodable calcium indicators

(2008) Janet E. McCombs et al.   METHODS

Chemical calcium indicators

(2008) R. Madelaine Paredes et al.   METHODS

Visualizing the spatial distribution of secondary metabolites produced by marine cyanobacteria and sponges via MALDI-TOF imaging

(2008) Eduardo Esquenazi et al.   Molecular BioSystems

Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient

(2008) Brian G. Fuller et al.   NATURE

Synthetic fluorescent sensors for studying the cell biology of metals

(2008) Dylan W Domaille et al.   Nature Chemical Biology

Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor

(2008) Damian J Wallace et al.   NATURE METHODS

Membrane recognition by phospholipid-binding domains

(2008) Mark A. Lemmon   NATURE REVIEWS MOLECULAR CELL BIOLOGY

N-Myristoyl transferase-mediated protein labelling in vivo

(2008) William P. Heal et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Transition States of Strain-Promoted Metal-Free Click Chemistry:  1,3-Dipolar Cycloadditions of Phenyl Azide and Cyclooctynes

(2008) Daniel H. Ess et al.   ORGANIC LETTERS

Fluorogenic and Chromogenic Rhodamine Spirolactam Based Probe for Nitric Oxide by Spiro Ring Opening Reaction

(2008) Hong Zheng et al.   ORGANIC LETTERS

A Hydrophilic Azacyclooctyne for Cu-Free Click Chemistry

(2008) Ellen M. Sletten et al.   ORGANIC LETTERS

Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors

(2008) M. Ouyang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A genetically encoded fluorescent sensor of ERK activity

(2008) C. D. Harvey et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters

(2008) S. A. Hires et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Imaging the glycome

(2008) S. T. Laughlin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A chemical method for fast and sensitive detection of DNA synthesis in vivo

(2008) A. Salic et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Exploring RNA transcription and turnover in vivo by using click chemistry

(2008) C. Y. Jao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cluster TOF-SIMS imaging: A new light for in situ metabolomics?

(2008) Sebastian Mas et al.   PROTEOMICS

In Vivo Imaging of Membrane-Associated Glycans in Developing Zebrafish

(2008) S. T. Laughlin et al.   SCIENCE

Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy

(2008) Christian W. Freudiger et al.   SCIENCE

Site-specific N-terminal labelling of proteinsin vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry

(2007) William P. Heal et al.   CHEMICAL COMMUNICATIONS

Rapid detection, discovery, and identification of post-translationally myristoylated proteins during apoptosis using a bio-orthogonal azidomyristate analog

(2007) Dale D. O. Martin et al.   FASEB JOURNAL

Identification of palmitoylated mitochondrial proteins using a bio-orthogonal azido-palmitate analogue

(2007) Morris A. Kostiuk et al.   FASEB JOURNAL