Review
Genetics & Heredity
Xuepeng Wang, Qiang Wu
Summary: This review summarizes the three pluripotent states of PSCs, their features, and the underlying molecular mechanisms that maintain their identities. The interrelation and transition among these states are also discussed. Understanding the divergence of pluripotent states is crucial for fully harnessing the potential of stem cells in regenerative medicine.
Article
Cell & Tissue Engineering
Liliya Nazlamova, Emma-Jane Cassidy, Jane C. Sowden, Andrew Lotery, Jorn Lakowski
Summary: A fluorescent reporter cell line for human cone photoreceptors was generated using CRISPR/Cas9 genome editing, allowing for tracking and analysis of cone cell development. The reporter cell line accurately labels cone cells and reveals migratory activity during in vitro differentiation.
Article
Cell Biology
Xinpeng Wen, Zesong Lin, Hao Wu, Lanrui Cao, Xudong Fu
Summary: In this study, the researchers identified a novel regulator, Zfp281, for the pluripotent-to-totipotent transition in mouse embryonic stem cells. They found that Zfp281 inhibits the transition by repressing transcripts upregulated during the 2C-like transition, and this inhibitory effect leads to impaired 2C-like transition ability in primed-state cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Satoshi Abe, Kazuhisa Honma, Akane Okada, Kanako Kazuki, Hiroshi Tanaka, Takeshi Endo, Kayoko Morimoto, Takashi Moriwaki, Shusei Hamamichi, Yuji Nakayama, Teruhiko Suzuki, Shoko Takehara, Mitsuo Oshimura, Yasuhiro Kazuki
Summary: Mammalian artificial chromosomes have significant applications in biomedical research, such as generating cell sources and creating humanized animal models. The stability of different sources of artificial chromosome vectors can vary and should be evaluated and chosen accordingly for the production of Tc animals.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Menno ter Huurne, Hendrik G. Stunnenberg
Summary: During early embryonic development, the rapid increase in cell number and the expression of genes that control developmental decisions are tightly regulated and interconnected processes. Transcription factors and signaling pathways play a crucial role in maintaining pluripotency and cell cycle progression, while canonical cell cycle regulators impact molecular networks controlling pluripotency and lineage specification.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Bo Ram Lee, Hyeon Yang, Sung June Byun, Tae Sub Park
Summary: Researchers established a reporter cell line system by specifically inserting the red fluorescent protein gene into the transcriptional start site of the NANOG gene using CRISPR-Cas9 technology in chicken DF1 cells. This study is of significant importance for practical applications in the field of avian biotechnology.
Article
Cell Biology
Chen Gao, Xiaolan Qi, Xin Gao, Jin Li, Yumin Qin, Yunjun Yin, Fei Gao, Tao Feng, Sen Wu, Xuguang Du
Summary: Screening with a CRISPR library under stringent conditions revealed key regulators of pluripotency exit, including previously reported genes and 17 unreported genes. Zfp771 and Olfr769 were found to be potentially involved in pluripotency exit.
Article
Multidisciplinary Sciences
Daniel F. Kaemena, Masahito Yoshihara, Meryam Beniazza, James Ashmore, Suling Zhao, Marten Bertenstam, Victor Olariu, Shintaro Katayama, Keisuke Okita, Simon R. Tomlinson, Kosuke Yusa, Keisuke Kaji
Summary: CRISPR/Cas9-mediated genome-wide knockout screening identified 24 reprogramming roadblock genes, among which depletion of the predicted KRAB zinc finger protein Zfp266 consistently enhanced murine iPSC generation in various reprogramming settings. ZFP266 was found to bind to Short Interspersed Nuclear Elements (SINEs) near the binding sites of OCT4, SOX2, and KLF4, inhibiting chromatin opening. Replacement of the KRAB co-suppressor with co-activator domains transformed ZFP266 from an inhibitor to a potent facilitator of iPSC reprogramming. The interaction between SINE and KRAB-ZFP is proposed to be a critical regulator of chromatin accessibility for efficient cellular identity changes.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Rowan M. Karvas, Laurent David, Thorold W. Theunissen
Summary: Trophoblasts play critical roles in blastocyst implantation and maternal-fetal communication during pregnancy. However, our understanding of human trophoblast biology is limited due to the scarcity of first-trimester placental tissue and the inadequacy of animal models. Recent advancements in deriving human trophoblast stem cells from pluripotent and somatic cell sources provide exciting opportunities to study the molecular mechanisms of placental development and pregnancy-related diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Olga A. Krasnova, Vitaly V. Gursky, Alina S. Chabina, Karina A. Kulakova, Larisa L. Alekseenko, Alexandra V. Panova, Sergey L. Kiselev, Irina E. Neganova
Summary: This study analyzed the morphology of human pluripotent stem cells to identify the best clones for further clinical applications. By analyzing different cell lines and phenotypes, informative markers were identified to separate the best clones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Hong-Fen Shen, Yong-Long Li, Shi-Hao Huang, Jia-Wei Xia, Zhi-Fang Yao, Gao-Fang Xiao, Ying Zhou, Ying-Chun Li, Jun-Wen Shi, Xiao-Lin Lin, Wen-Tao Zhao, Yan Sun, Yu-Guang Tian, Jun-Shuang Jia, Dong Xiao
Summary: This study successfully generated Oct4-GFP miPSCs from mouse somatic cells, which exhibited similar morphology, proliferation, and gene expression to mESCs. Oct4-GFP miPSCs could differentiate and retain pluripotency, and their pluripotency could be monitored in real-time using a GFP reporter system.
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Pei Lu, Mao Li, Donghui Zhang, Wei Jiang
Summary: Long noncoding RNAs (lncRNAs) play important roles in regulating cell fate in pluripotent stem cells. They have four major functional mechanisms based on different types of cofactors. Further understanding of lncRNA-based regulation will provide more insights into the drivers manipulating cell fate.
Article
Multidisciplinary Sciences
Takashi Yoshino, Takahiro Suzuki, Go Nagamatsu, Haruka Yabukami, Mika Ikegaya, Mami Kishima, Haruka Kita, Takuya Imamura, Kinichi Nakashima, Ryuichi Nishinakamura, Makoto Tachibana, Miki Inoue, Yuichi Shima, Ken-ichirou Morohashi, Katsuhiko Hayashi
Summary: Novel methods have been developed to generate functional mouse oocytes from pluripotent stem cell-derived primordial germ cell-like cells (PGCLCs) and fetal ovarian somatic cell-like cells (FOSLCs). When FOSLCs are aggregated with PGCLCs, they enter meiosis and produce oocytes capable of fertilization and development into live offspring, providing a method for in vitro oocyte production and follicle generation for a better understanding of mammalian reproduction.
