Article
Chemistry, Multidisciplinary
Danyang Wang, Ana Shalamberidze, Emilia Arguello, Byron W. Purse, Ralph E. Kleiner
Summary: The article introduces a platform for fluorescence imaging of bulk RNA dynamics in living cells and uses this platform to study RNA metabolism and localization during oxidative stress. The study found that bulk RNA turnover is accelerated upon oxidative stress treatment and identified new cytoplasmic RNA granules that co-localize with RNA helicase.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Immunology
Anette T. Hansen Selno, Vadim V. V. Sumbayev, Bernhard F. F. Gibbs
Summary: Basophils play a crucial role in allergies and Th2-driven diseases by releasing inflammatory and immunomodulatory mediators. The responsiveness of basophils to IgE-mediated activation may be regulated by the expression of SERCA2, a calcium-regulating protein. This study found an inverse correlation between SERCA2 expression and basophil responsiveness, suggesting that SERCA2 may be an additional regulator of basophil reactivity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Physical
Jianqiao Chang, Yu Zhang, Yike Li, Ziwei Han, Fei Tian, Chao Liu, Qiang Feng, Yuguang Wang, Jiashu Sun, Lu Zhang
Summary: This study introduces a multilayer ratiometric fluorescent nanomachine for live-cell perturbation and imaging of mRNA at single cell resolution, offering great potential for quantifying mRNA expression and modulating and imaging intratumoral mRNA.
Article
Multidisciplinary Sciences
Hela Benaissa, Karim Ounoughi, Isabelle Aujard, Evelyne Fischer, Rosette Goiame, Julie Nguyen, Alison G. Tebo, Chenge Li, Thomas Le Saux, Giulia Bertolin, Marc Tramier, Lydia Danglot, Nicolas Pietrancosta, Xavier Morin, Ludovic Jullien, Arnaud Gautier
Summary: Fluorescent reporters covering the visible spectrum are essential for imaging living cells and organisms. The authors have developed a collection of fluorogenic chromophores spanning from blue to red using a single engineered protein tag. This tool allows for tuning fluorescence color and properties, providing versatility for imaging proteins in live cells and optimizing FRET biosensors.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Daiki Matsubara, Yuhki Yanase, Kaori Ishii, Shunsuke Takahagi, Akio Tanaka, Koichiro Ozawa, Michihiro Hide
Summary: This article discusses the role of basophils in the pathogenesis of chronic spontaneous urticaria (CSU), as well as the critical role of the complement system in its activation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sukhvinder Dhiman, Rasdeep Kour, Satwinderjeet Kaur, Prabhpreet Singh, Subodh Kumar
Summary: A new fluorescent probe DMAS-Si was reported in this study for the simultaneous detection of mitochondrial viscosity and F- ion through different signals, showing significant potential for investigating the physiological state of mitochondria.
BIOORGANIC CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ling-Li Wang, Jian-Ying Bai, Xi-Feng Li, Ming-Hua Zheng, Yu Miao, Jing-Yi Jin
Summary: A new fluorescent probe QBN was developed for detection of HOCl and NO, and successfully achieved simultaneous imaging of both exogenous and endogenous HOCl and NO in live cells.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Inorganic & Nuclear
He Tian, Adam C. Sedgwick, Hai-Hao Han, Sajal Sen, Guo-Rong Chen, Yi Zang, Jonathan L. Sessler, Tony D. James, Jia Li, Xiao-Peng He
Summary: Lipid droplets (LDs) are essential organelles for lipid and energy homeostasis. Despite progress in understanding LDs, there are still many fundamental questions unanswered. Effective imaging agents can help bridge the knowledge gap and facilitate the development of new therapeutics.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Microbiology
Caetanie F. Tchagang, Thien-Fah Mah, Francois-Xavier Campbell-Valois
Summary: Pseudomonas aeruginosa, a bacteria commonly found in the airways of individuals with cystic fibrosis, forms biofilms resistant to immune clearance and antibiotic treatment. In low-oxygen conditions, P. aeruginosa can use nitrate instead of oxygen to grow. Current fluorescent reporters for studying P. aeruginosa require oxygen for their chromophore maturation, limiting their use in anaerobic conditions. This study evaluated alternative fluorescent proteins and identified two that successfully labeled P. aeruginosa in anoxic environments, enabling the study of P. aeruginosa in biofilms and similar low-oxygen niches.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Spectroscopy
Baoqiang Liu, Shanshan Wei, Enqi Liu, Hongyuan Zhang, Pengju Lu, Jiali Wang, Guoying Sun
Summary: A simple and convenient approach was designed to prepare fluorescent N-doped carbon dots for folic acid (FA) detection. The N-CDs showed excellent hydrophilicity, high photostability, and outstanding biocompatibility, making them a reliable quantitative probe for FA analysis in the field of biosensing. Cell experiments confirmed low toxicity and good cell imaging performance of the N-CDs, indicating their potential for practical applications in FA detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Zhining Sun, Rigumula Wu, Bin Zhao, Rilee Zeinert, Peter Chien, Mingxu You
Summary: A RNA-based fluorescent sensor has been developed for live-cell imaging of (p)ppGpp, allowing direct monitoring of cellular accumulation and dynamics of (p)ppGpp levels. These sensors provide unprecedented information on cell-to-cell variation and can be broadly adapted to study bacterial stringent response.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Guodong Hu, Miao Zhong, Jintao Zhao, Hao Gao, Lu Gan, Hong Zhang, Shengxiang Zhang, Jianguo Fang
Summary: Cellular redox homeostasis is primarily regulated by the ratio of thiols and disulfides, with reversible thioldisulfide exchange reactions being crucial. Detecting protein disulfides (PDS) poses challenges due to dynamic exchanges of thiols and disulfides. Researchers have designed a probe that selectively recognizes PDS in live organisms, revealing an increase of PDS in mouse brains of a stroke model.
Article
Chemistry, Applied
Hoi-Yi Sit, Jie-Ren Deng, Wing-Cheung Chan, Ben Chi-Bun Ko, Man-Kin Wong
Summary: A new type of quinolizinium-based fluorescent probes has been developed for cell imaging with tunable pH responsiveness. These probes exhibit turn On fluorescence response upon acidification through modulation of photoinduced electron transfer (PET) from the N,N-dialkyl aniline substituent to the quinolizinium core skeleton. They demonstrate tunable emission properties and pH responsiveness with pKa ranging from <2.1 to 6.1, thanks to the steric effect of N,N-dialkyl anilines. Successful application for in vivo pH sensing in lysosome in live cells has been achieved.
Article
Chemistry, Medicinal
Reinis Putralis, Ksenija Korotkaja, Martins Kaukulis, Zhanna Rudevica, Juris Jansons, Olga Nilova, Martins Rucins, Laura Krasnova, Ilona Domracheva, Mara Plotniece, Karlis Pajuste, Arkadij Sobolev, Felikss Rumnieks, Laura Bekere, Anna Zajakina, Aiva Plotniece, Gunars Duburs
Summary: A set of styrylpyridinium (SP) compounds were synthesized and studied for their spectroscopic and cell labelling properties. These compounds exhibited intense near-infrared fluorescence and high cytotoxicity towards cancer cells.
Article
Chemistry, Multidisciplinary
Heng Tang, Junran Peng, Shuang Peng, Qi Wang, Xin Jiang, Xiaocheng Xue, Yanxin Tao, Limin Xiang, Quanjiang Ji, Song-Mei Liu, Xiaocheng Weng, Xiang Zhou
Summary: This study presents an efficient RNA imaging method based on the CRISPR-dPspCas13b system, which allows for imaging and tracking of endogenous RNA in live cells. The method utilizes fluorescent RNA aptamers with reduced background fluorescence, providing a simple and sensitive approach for RNA imaging, and color switching can be easily achieved by changing fluorogenic dye analogues.
Article
Chemistry, Multidisciplinary
Hong-Guen Lee, Avinash Dhamija, Chandan K. Das, Kyeng Min Park, Young-Tae Chang, Lars V. Schaefer, Kimoon Kim
Summary: This study presents synthetic monosaccharide channels based on shape-persistent organic cages, porphyrin boxes (PBs), for facile transmembrane transport of glucose and fructose. PBs have a higher transport rate for glucose and fructose compared to disaccharides, demonstrated by enzyme assays and molecular dynamics simulations. The transport rate can be controlled by changing the length of the alkyl chains on the cage windows. When the natural glucose transporter GLUT1 is blocked, PBs are able to transport glucose across living-cell membranes and enhance cell viability. Time-dependent live-cell imaging and MTT assays confirm the cyto-compatibility of PBs. The monosaccharide-selective transport ability of PBs resembles that of natural glucose transporters (GLUTs), which play critical roles in biological functions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Applied
Thanawat Chaiprasert, Supphachok Chanmungkalakul, Yujia Liu, Teeraya Bureerug, Komthep Silpcharu, Masafumi Unno, Liu Xiaogang, Vuthichai Ervithayasuporn, Young-Tae Chang, Paitoon Rashatasakhon
Summary: Two new all-cis pyrene-substituted cyclic tetrasiloxanes (JR-01 and JR-02) have been synthesized and studied as selective metal ion sensors. Both compounds exhibit solvent-and concentration-dependent excimer fluorescence emission at 482 nm. They also show highly selective fluorescence quenching by Au(III) ion with detection limits of 0.9 and 0.8 mu M for JR-01 and JR-02, respectively. Data from various experiments indicate a plausible mechanism involving the formation of a non-emissive Au(III) complex.
Article
Chemistry, Analytical
Mousumi Baruah, Haw-Young Kwon, Heewon Cho, Young-Tae Chang, Animesh Samanta
Summary: The differentiation of macrophage phenotypes is crucial for accurate diagnosis and treatment of inflammatory diseases. A fluorescent probe called PMS-T has been developed to distinguish M1 from M0 and M2 macrophages by detecting the levels of hypochlorite (OCl-). This probe has the potential to serve as a chemical tool for monitoring inflammatory diseases, such as rheumatoid arthritis, and overcome the limitations of immunohistochemistry.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Lorena Mendive-Tapia, Laia Miret-Casals, Nicole D. Barth, Jinling Wang, Anne de Bray, Massimiliano Beltramo, Vincent Robert, Christophe Ampe, David J. Hodson, Annemieke Madder, Marc Vendrell
Summary: This study describes the rational design and characterization of a new acid-resistant BODIPY-based amino acid (Trp-BODIPY PLUS) and its application in solid-phase synthesis of fluorescent bioactive peptides. The amino acid retains the binding capabilities of both short linear and cyclic peptides and shows notable turn-on fluorescence emission upon target binding for wash-free imaging. Trp-BODIPY PLUS was used to prepare fluorogenic kisspeptin-based probes and visualize the expression and localization of GPR54 receptors in human cells and whole mouse pancreatic islets by fluorescence imaging.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Pau Nadal Rodriguez, Ouldouz Ghashghaei, Anna M. M. Schoepf, Sam Benson, Marc Vendrell, Rodolfo Lavilla
Summary: Charting the chemical reaction space around the combination of carbonyls, amines, and isocyanoacetates allows the discovery of new multicomponent processes leading to a variety of unsaturated imidazolone scaffolds. The resulting compounds exhibit the chromophore of the green fluorescent protein and the core of the natural product coelenterazine. Despite the competitive nature of the pathways involved, general protocols provide selective access to desired chemotypes. Furthermore, unprecedented reactivity at the C-2 position of the imidazolone core is described, directly affording C, S, and N-derivatives featuring natural products (e.g. leucettamines), potent kinase inhibitors, and fluorescent probes with suitable optical and biological profiles.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Medicine, Research & Experimental
Tusar Kanta Acharya, Shamit Kumar, Tejas Pravin Rokade, Young-Tae Chang, Chandan Goswami
Summary: The activation of T cells is influenced by temperature and intracellular calcium signaling. TRPV4, a non-selective calcium channel, is expressed in T cells and located in their mitochondria. Activation of TRPV4 increases mitochondrial calcium levels and affects mitochondrial temperature and metabolism.
Article
Chemistry, Multidisciplinary
Deborah Seah, Zhiming Cheng, Marc Vendrell
Summary: Optical imaging is now an essential technology in clinical settings. The development of cell-targeted and highly sensitive materials, the validation of specific disease biomarkers, and the advancement of clinically compatible instrumentation have revolutionized the application of optical imaging. For instance, cancer-targeted molecular imaging agents have been used in trials and routine clinical practice to define tumor margins and detect invisible lesions, resulting in improved surgical resection of malignant tissues without damaging viable structures. In this Perspective, key research advances in chemistry, biology, and engineering that have accelerated the translation of optical imaging technologies for human patients are summarized. Finally, the paper provides comments on potential research areas that will contribute to the next generation of translational optical imaging technologies.
Article
Multidisciplinary Sciences
Katia Monsorno, Kyllian Ginggen, Andranik Ivanov, An Buckinx, Arnaud L. Lalive, Anna Tchenio, Sam Benson, Marc Vendrell, Angelo D'Alessandro, Dieter Beule, Luc Pellerin, Manuel Mameli, Rosa Chiara Paolicelli
Summary: Microglia can import lactate as a metabolic fuel and lactate promotes lysosomal acidification in these cells. Loss of MCT4 in microglia leads to impaired synapse pruning and behavioral defects.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Takeru Yamazaki, Xiao Liu, Young-Tae Chang, Satoshi Arai
Summary: Fluorescence thermometry is a microscopy technique that records temperature changes through fluorescence signals. We developed small-molecule thermometers for quantitative temperature mapping using fluorescence lifetime imaging (FLIM). However, due to expensive instrumentation, we investigated the applicability and limitations of fluorescence intensity (FI) analysis for thermometry. Comparing FI and FLIM-based thermometry, we concluded that FI-based thermometry can be used qualitatively if there are no critical shape changes or dye leakage from the target organelle.
Article
Radiology, Nuclear Medicine & Medical Imaging
Seul-Ki Mun, Hyun Bo Sim, Ji Yeon Han, Hyeongyeong Kim, Dae-Han Park, Dong-Jo Chang, Sung-Tae Yee, Young-Tae Chang, Jong-Jin Kim
Summary: The development of efficient biomarkers and probes for monitoring and treating cancer, specifically metastatic cancer, is crucial for patient outcomes and drug discovery.
Meeting Abstract
Oncology
Nicole D. Barth, Floris J. van Dalen, Camille Duran, Utsa Karmakar, Lorena Mendive-Tapia, Takanori Kitamura, Martjin Verdoes, John S. Condeelis, Maja Oktay, Marc Vendrell, Valerie Brunton, David Entenberg
Article
Medicine, Research & Experimental
Sam Benson, Alex Kiang, Charles Lochenie, Navita Lal, Syam Mohan P. C. Mohanan, Gareth O. S. Williams, Kevin Dhaliwal, Bethany Mills, Marc Vendrell
Summary: Bacterial infections pose significant challenges to human health, leading to high antibiotic usage, morbidity, hospitalizations, and approximately 8 million deaths worldwide annually. The overuse of antibiotics and lack of antimicrobial innovations have resulted in antimicrobial resistant pathogens that may reverse the progress of modern medicine. Photodynamic therapeutics have the potential to kill bacteria, but there are limited agents that can target pathogens effectively with minimal off-target effects.
Review
Chemistry, Multidisciplinary
Marco Bertolini, Man Sing Wong, Lorena Mendive-Tapia, Marc Vendrell
Summary: T cells play crucial roles in adaptive response and tissue homeostasis in the immune system. Smart probes, including small molecule fluorophores and nanoconstructs, have been developed to image T cells in tumors and inflammation sites by targeting metabolic and enzymatic biomarkers as well as specific surface receptors. This review summarizes recent advancements in the design, synthesis, and application of smart probes for imaging T cells, and discusses their use in monitoring T cell response to anti-cancer immunotherapies.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Tsuyoshi Oyama, Lorena Mendive-Tapia, Verity Cowell, Adelina Kopp, Marc Vendrell, Lutz Ackermann
Summary: Fluorescent labeling of peptides catalyzed by non-toxic manganese(i) via C(sp(2))-H alkenylation enables the selective and site-specific labeling of peptides, providing access to novel near-infrared nitrobenzodiazole-based peptide fluorogenic probes.
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
