4.7 Article

Ionic liquids promote PCR amplification of DNA

Journal

CHEMICAL COMMUNICATIONS
Volume 48, Issue 43, Pages 5325-5327

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc31740k

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Funding

  1. National Science Council of Taiwan, ROC [NSC-100-2113-M-194-003-MY3, NSC-99-2811-M-194-027]
  2. Advanced Institute for Manufacturing with High-Tech Innovations (AIM-HI at CCU)
  3. NSC of Taiwan

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A bicyclic imidazolium ionic liquid (4d), [b-4C-im][Br], was found to be highly effective not only for promoting PCR of GC-rich DNA by minimizing non-specific amplification, but also for facilitating PCR of normal-GC DNA under mild conditions.

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