Journal
CHEMICAL COMMUNICATIONS
Volume 47, Issue 38, Pages 10806-10808Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cc13885e
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Glycosynthase mutants of Rhodococcus sp. endo-glycoceramidase II efficiently synthesize complex glycosphingolipids. Glycosyl fluoride donors may be assembled via sequential glycosyltransferase-catalysed glycosylation of lactosyl fluoride. Alternatively, lactosyl fluoride may be coupled to sphingosine prior to subsequent glycosylation steps.
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