Journal
CHEMICAL COMMUNICATIONS
Volume 46, Issue 26, Pages 4683-4685Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c002666b
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Funding
- National Research Laboratory Program [R0A-2005-000-10039-0]
- Chemical Genomics Grant [M10526020002-08N2602-00210]
- National Research Foundation [KRF-2005-084-C00017]
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Using a single-molecule fluorescence method uniquely suitable for binding assay, alternating-laser excitation fluorescence resonance energy transfer (ALEX-FRET), we accurately measured the cleavage rate of 8-17 deoxyribozyme, an RNA-cleaving enzyme, at the single-molecule level in real time with a minimum consumption of samples, i.e., at least three orders of magnitude smaller than used in the conventional ensemble FRET method.
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