Journal
CHEMICAL & PHARMACEUTICAL BULLETIN
Volume 56, Issue 3, Pages 247-249Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/cpb.56.247
Keywords
thermodynamics; DNA condensation; isothermal titration calorimetry; supercoiled DNA; Mn2+ binding
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Interaction between Mn2+ ion and the two forms of DNA duplex (supercoiled and linearized pUC119 DNA) in solution has been examined by isothermal titration calorimetry. Although DNA condensation reaction heat was observed at 323 K, this was not the case at 298 K. DNA condensation was entropically driven and supercoiled DNA was found to be more susceptive. The enthalpy of DNA condensation is estimated 0.42 kJ/mol for both DNA forms. Conversely, the entropy of DNA condensation was 0.13 kJ/mol K for supercoiled DNA, and 0.12 kJ/mol K for linearized DNA. The difference of entropy is attributable to their DNA conformation.
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