Directed Evolution by Using Iterative Saturation Mutagenesis Based on Multiresidue Sites

A New Type of Stereoselectivity in Baeyer-Villiger Reactions: Access toE- andZ-Olefins

(2013) Zhi-Gang Zhang et al.   ADVANCED SYNTHESIS & CATALYSIS

The Importance of Additive and Non-Additive Mutational Effects in Protein Engineering

(2013) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Quantum Mechanical/Molecular Mechanical Study on the Enantioselectivity of the Enzymatic Baeyer–Villiger Reaction of 4-Hydroxycyclohexanone

(2013) Iakov Polyak et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Many Pathways in Laboratory Evolution Can Lead to Improved Enzymes: How to Escape from Local Minima

(2012) Yosephine Gumulya et al.   CHEMBIOCHEM

Protein Engineering of Stereoselective Baeyer-Villiger Monooxygenases

(2012) Zhi-Gang Zhang et al.   CHEMISTRY-A EUROPEAN JOURNAL

Quantum Mechanical/Molecular Mechanical Study on the Mechanism of the Enzymatic Baeyer–Villiger Reaction

(2012) Iakov Polyak et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reducing Codon Redundancy and Screening Effort of Combinatorial Protein Libraries Created by Saturation Mutagenesis

(2012) Sabrina Kille et al.   ACS Synthetic Biology

When Second Best Is Good Enough: Another Probabilistic Look at Saturation Mutagenesis

(2011) Yuval Nov   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Baeyer−Villiger Monooxygenases: More Than Just Green Chemistry

(2011) Hannes Leisch et al.   CHEMICAL REVIEWS

Strategy and success for the directed evolution of enzymes

(2011) Paul A Dalby   CURRENT OPINION IN STRUCTURAL BIOLOGY

Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis

(2011) David P Nannemann et al.   Future Medicinal Chemistry

Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library

(2011) A. G. Sandstrom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering of Biocatalysts: from Evolution to Creation

(2011) Maureen B. Quin et al.   ACS Catalysis

Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

(2010) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Optimizing non-natural protein function with directed evolution

(2010) Eric M Brustad et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering of Enzymes for Selective Catalysis

(2010) Nikhil U. Nair et al.   CURRENT ORGANIC CHEMISTRY

Design and Evolution of Biocatalysts

(2010) Sang-Chul Lee et al.   CURRENT ORGANIC CHEMISTRY

Random Mutagenesis Methods for In Vitro Directed Enzyme Evolution

(2010) Nikolaos Labrou   CURRENT PROTEIN & PEPTIDE SCIENCE

Enzyme-mediated oxidations for the chemist

(2010) Frank Hollmann et al.   GREEN CHEMISTRY

Iterative Saturation Mutagenesis Accelerates Laboratory Evolution of Enzyme Stereoselectivity: Rigorous Comparison with Traditional Methods

(2010) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed Evolution of an Enantioselective Enoate-Reductase: Testing the Utility of Iterative Saturation Mutagenesis

(2009) Despina J. Bougioukou et al.   ADVANCED SYNTHESIS & CATALYSIS

Advances in generating functional diversity for directed protein evolution

(2009) Amol V Shivange et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Baeyer–Villiger monooxygenases: recent advances and future challenges

(2009) Daniel E Torres Pazmiño et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Laboratory Evolution of Robust and Enantioselective Baeyer−Villiger Monooxygenases for Asymmetric Catalysis

(2009) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Enzyme engineering for enantioselectivity: from trial-and-error to rational design?

(2009) Linda G. Otten et al.   TRENDS IN BIOTECHNOLOGY

Protein Design by Directed Evolution

(2008) Christian Jäckel et al.   Annual Review of Biophysics

Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates

(2008) Joaquin Sanchis et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Directed evolution of aldolases for exploitation in synthetic organic chemistry

(2008) Amanda Bolt et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

(2008) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

Advances in laboratory evolution of enzymes

(2008) Shimon Bershtein et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries

(2008) A. E. Firth et al.   NUCLEIC ACIDS RESEARCH

‘Designer reagents’ recombinant microorganisms: new and powerful tools for organic synthesis

(2008) Margaret M. Kayser   TETRAHEDRON

Selective Baeyer–Villiger oxidation of racemic ketones in aqueous–organic media catalyzed by phenylacetone monooxygenase

(2008) Cristina Rodríguez et al.   TETRAHEDRON-ASYMMETRY

Enzyme optimization: moving from blind evolution to statistical exploration of sequence–function space

(2008) Richard J. Fox et al.   TRENDS IN BIOTECHNOLOGY