Article
Biophysics
Yuanyuan Zhu, Jian Wu, Qingli Zhou
Summary: Functional DNA (fDNA) refers to DNA molecules that have specific binding ability or catalytic function, with advantages of simple synthesis, low cost, low toxicity, high chemical stability, recognition specificity, and biocompatibility. However, the limited sensitivity of fDNA sensors to trace targets is a major challenge, especially when there is low affinity between fDNA and the targets. Various nucleic acid signal amplification strategies (NASAS) have been explored to improve the limit of detection of fDNA. This review summarizes four NASAS (hybridization chain reaction, entropy-driven catalysis, rolling circle amplification, CRISPR/Cas system) and their corresponding design principles, as well as the principle and application of fDNA sensors integrated with signal amplification strategies for the detection of non-nucleic acid targets. The main challenges and application prospects of NASAS-integrated fDNA biosensing system are also discussed.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Bo Shen, Li Li, Changjin Liu, Xinmin Li, Xinyu Li, Xiaoxue Cheng, Haiping Wu, Tiantian Yang, Wei Cheng, Shijia Ding
Summary: An ultrasensitive electrochemiluminescent (ECL) biosensor was constructed for accurate detection of exosomal microRNAs (exomiRNAs), using a three-dimensional (3D) walking nanomotor-mediated CRISPR/Cas12a and tetrahedral DNA nanostructures (TDNs)-modified nanoemitters (TCPP-Fe@HMUiO@Au-ABEI). The biosensor achieved a limit of detection down to 273.20 aM and successfully discriminated breast cancer patients.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Marta Broto, Michael M. Kaminski, Christopher Adrianus, Nayoung Kim, Robert Greensmith, Schan Dissanayake-Perera, Alexander J. Schubert, Xiao Tan, Hyemin Kim, Anand S. Dighe, James J. Collins, Molly M. Stevens
Summary: This study combines CRISPR-Cas reaction with nanozyme-linked ELISA to achieve quantitative detection of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). The study shows that CrisprZyme is adaptable to lateral flow reading and different Cas enzymes, and can detect various non-coding RNAs. The platform can be used to identify disease patients and monitor cellular differentiation. CrisprZyme can serve as a universally applicable signal catalyst for CRISPR-based diagnostics, expanding the target range of preamplification-free, quantitative detection.
NATURE NANOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Xiangcheng Lin, Mengmeng Zhao, Mingyue Li, Juan Long, Jing Zhang, Fang Yu, Fen Xu, Lixian Sun
Summary: A single molecule detection method based on mass spectrometry was developed to detect nucleic acids using single liposome particles. This ultra-sensitive technique allows the detection of extremely low concentrations of nucleic acids.
Article
Chemistry, Analytical
Hao Jiang, Yuan Li, Xuefei Lv, Yulin Deng, Xiaoqiong Li
Summary: Nucleic acid amplification techniques, such as polymerase chain reaction (PCR) and isothermal amplification, are important for nucleic acid detection. However, PCR is limited by temperature control and isothermal amplification has its own limitations. Efforts to integrate enzymes and amplification techniques have the potential to overcome these limitations. This review provides a comprehensive summary of cascade amplification, including design fundamentals, signal generation, evolution, applications, challenges, and trends.
Article
Chemistry, Analytical
Tong Luo, Jiacheng Li, Yao He, Hui Liu, Zhiwei Deng, Xi Long, Qingqing Wan, Jiacheng Ding, Zan Gong, Yanjing Yang, Shian Zhong
Summary: In this study, we developed a novel CRISPR-based biosensor by combining CRISPR/Cas12a with Au-nanobeacon, which allows for direct and rapid detection of nucleic acids in complex biological fluids with high sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Haobo Sun, Lisheng Qian, Jinming Kong, Xueji Zhang
Summary: A novel electrochemical biosensor platform with triple helix structure switch and double signal amplification strategy was proposed for nucleic acid detection, achieving ultra-sensitivity and high selectivity. The biosensor showed a detection limit as low as 1.954 aM and a linear range of 7 orders of magnitude. Additionally, it demonstrated excellent anti-interference ability when analyzing DNA in serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Hongfei He, Yan Zhou, Bin Chen, Yi Zhang, Xiaowu Zhong, Lei Xu, Bin Guo, Chong Yin, Xi Zhou, Qingrong Li, Zhen Huang, Guangcheng Luo, Xiaolan Guo
Summary: A colorimetric isothermal sensing platform (SFMC) with specific signal filtration and magnification has been developed for ultrasensitive detection of DNA and RNA. This platform can detect target DNA as low as 5 copies per reaction and target RNA as low as 10 copies per reaction by naked eyes. It has also been successfully used for the detection of African swine fever virus (ASFV) and SARS-CoV-2.
Article
Chemistry, Analytical
Qiuyuan Lin, Yuanwei Cao, Guobin Han, Wen Sun, Wenhao Weng, Hui Chen, Haoyi Wang, Jilie Kong
Summary: We report a general and programmable diagnostic platform that combines mesophilic Clostridium perfringens Argonaute (CpAgo) with exponential isothermal amplification (EXPAR) for the rapid and sensitive detection of multiple miRNAs with single-nucleotide discrimination in one pot. The CpAgo-based One-Pot (COP) assay achieved a limit of detection of 1 zMmiRNA within 30 min and showed superior analytical performance in distinguishing colorectal cancer patients from healthy individuals. This programmable, one-pot, multiplex, rapid, and specific strategy holds great promise in scientific research and clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Review
Food Science & Technology
Tai-Yong Kim, Xiaoning Zhu, Se-Min Kim, Jeong-A Lim, Min-Cheol Lim, Ke Luo
Summary: Viruses are major pathogens in food poisoning, and the development of foodborne virus detection technologies for the entire food distribution chain is crucial for food safety. Polymerase chain reaction (PCR) is the gold standard method for monitoring food contamination by viruses, but it is time-consuming and vulnerable to inhibitors in food samples. Optical and electrical readout biosensors based on nucleic acid isothermal amplification technology and nanomaterials have emerged as alternatives, offering portability and on-site operation. However, the complexity of food components and lack of stability studies on residual food components limit the practical application of biosensors as a universal detection method.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Nan Zhang, Jingrong Li, Boshi Liu, Di Zhang, Chengyu Zhang, Yuheng Guo, Xinhong Chu, Wenting Wang, Haixia Wang, Xiaohui Yan, Zheng Li
Summary: This paper reviews the biological and ecological toxicities caused by small molecular contaminants in food and environment, as well as the development of aptasensors in recent years. It also discusses in detail the signal enhancing strategies based on nanomaterials and nucleic acid amplification, along with the challenges and future trends in the application of these strategies.
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Shengjun Bu, Qianyu Zhou, Liming Liu, Chunyang Zhou, Zhuo Hao, Jiayu Wan, Chunying Pang
Summary: The present study describes a novel technology called TPIA that enables target nucleic acid detection. By using specific design strategy and fluorescence techniques, this system provides a versatile tool with great potential for biomedical research and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Shu-Ying Ye, Chang-Gang Pan, Yu-Hua Dai, Guo-Xi Liang
Summary: An ultrasensitive biosensor for the detection of telomerase activity was constructed utilizing gold@Carbon dots nanohybrids (Au@CDs) as a high efficiency electrochemiluminescence (ECL) emitter and nicking enzyme assisted signal amplification (NESA) technology. By employing a dual signal amplification strategy, the biosensor showed excellent sensitivity and detection limit for telomerase activity.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Wei-Wei Liu, Xiao-Long Zhang, Liang Zhu, Sai Xu, Ya-Qin Chai, Zhao-Hui Li, Ruo Yuan
Summary: In this study, a mismatch-fueled catalytic hairpin assembly (MCHA) with higher amplification efficiency and faster rate than catalytic hairpin assembly (CHA) was engineered. MCHA, triggered by input target miRNA-21, generates abundant output products with significantly shorter reaction time and higher amplification efficiency, overcoming the limitations of traditional CHA.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Chong Duan, Jing-Jing Hu, Rui Liu, Jun Dai, Lizhen Yuan, Fan Xia, Xiaoding Lou
Summary: Cell membrane transport is a crucial step for bioprobes to achieve diagnosis, imaging, and therapy in cells. However, there is a trade-off between anchoring and internalization steps, affecting the efficiency of membrane transport. In this study, a strategy was proposed to regulate the membrane affinity of multi-module probes, enabling strong affinity during anchoring and weak affinity during internalization. This approach increased the membrane transport efficiency and addressed the trade-off problem.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Yichuan Wang, Xiaojin Zhang, Yu Dai, Fan Xia
Summary: We constructed a photo-modulated organometallic nanozyme by anchoring azobenzene-modified triethylenetetramine (Azo-N4) to the surface of beta-cyclodextrin-capped gold nanoparticles (AuNP@CDs) through host-guest interaction, followed by Zn(II) coordination to form organometallics. The catalytic site derives from the outer organometallic layer. The nanozyme catalyzes the hydrolysis reaction and the catalytic signal is used to detect Zn(II). Due to the photoisomerization of azobenzene, the supramolecular assembly of host-guest interaction is modulated by light, leading to cascade catalysis and recovery of AuNP@CDs.
CHEMICAL ENGINEERING JOURNAL
(2023)
Editorial Material
Chemistry, Multidisciplinary
Yuchen Wu, Fan Xia, Shutao Wang
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
(2023)
Editorial Material
Chemistry, Multidisciplinary
Lei Xu, Linfeng Chen, Fan Xia
Summary: A recent study found that a superhydrophobic surface can significantly improve the pervaporation separation of ethanol-water mixture, which has broad implications for the design of new separation membranes.
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
(2023)
Article
Materials Science, Multidisciplinary
Wanglong Zhang, Yu Dai, Fan Xia, Xiaojin Zhang
Summary: Inspired by the adhesion mechanism of mussels, a universal strategy of interfacial molecular lock (IML) for hydrogel adhesion is proposed. IML is composed of organic (low-molecular-weight) molecules containing catechol groups, which can easily penetrate into hydrogels. The principle of IML relies on the combination of various interactions. IML can adapt to interfacial chemistry of hydrogels on different substrates and achieve reversible adhesion without external regulation. The molecular micro-polymerization further enhances the stability and strength of hydrogel adhesion. IML is expected to expand hydrogel adhesion in various applications.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Jun Dai, Simin Wei, Jiarong Xu, Huiying Xue, Zhaojun Chen, Meng Wu, Wei Chen, Xiaoding Lou, Fan Xia, Shixuan Wang
Summary: A microneedle-based device combined with aggregation-induced emission (AIE) photosensitizers (PSs) was constructed to achieve enhanced metronomic photodynamic therapy (mPDT) for cancer. The strong anti-photobleaching property of the AIE PS allows for superior photosensitivity even after prolonged light exposure. The delivery of the AIE PS to the tumor through the microneedle device provides better treatment outcomes and convenience.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Xia Wu, Jing-Jing Hu, Lulu Chen, Zhaojun Chen, Tingting Wang, Feng Wu, Jun Dai, Fan Xia, Xiaoding Lou
Summary: A novel strategy is proposed to regulate nuclear proteins by targeting their signaling pathways in the cytoplasm, bypassing the challenge of directly entering into nuclei. A multifunctional complex PKK-TTP/hs is used to reduce the import of nuclear proteins through gene silencing in the cytoplasm, while promoting the export of nuclear proteins by generating reactive oxygen species under light irradiation. This dual-regulatory pathway successfully decreased nuclear protein (hTERT proteins) in vivo (42.3%).
Article
Chemistry, Multidisciplinary
Zhi Zheng, Haiyang Li, Liang Hai, Ruiqi Ma, Rui Liu, Chunpeng Zhai, Zongdong Sun, Fei Wang, Ying Ma, Qingguo Xie, Xiaoding Lou, Tianyou Zhai, Fan Xia
Summary: 2D Cs3Bi2Br9 nanoflakes possess excellent optical/electrical properties and stability, making them promising for high sensitivity X-ray detection. An Ag+ assisted inversion temperature crystallization method was proposed to synthesize the nanoflakes, which passivates Br- vacancies and enhances structure stability. The high crystallinity nanoflakes exhibit direct bandgap characteristic and high mobility lifetime, enabling the development of highly sensitive optoelectronic devices.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Bochao Chen, Jing-Jing Hu, Hanzhi Ouyang, Wei Zhang, Jun Dai, Liang Xu, Fan Xia, Xiaoding Lou
Summary: A peptide-conjugated probe was designed to induce cell apoptosis and monitor therapeutic responses by imaging aggregated proteins.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Xiaojin Zhang, Meihua Lin, Yu Dai, Fan Xia
Summary: Nanoporesensing technology is a promising analysis method with various advantages such as simplicity, high sensitivity, fast output, and label-free detection. It has been widely used in protein analysis, gene sequencing, biomarker detection, and other fields. By utilizing the confined space of the nanopore, this technology enables real-time tracking of dynamic interactions and chemical reactions at the single-molecule level, providing insights into the mechanisms of these processes. This paper reviews the development of biological nanopores and solid-state nanopores/nanochannels in stochastic sensing of dynamic interactions and chemical reactions, aiming to stimulate researchers' interest and promote the advancement of this field.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ya Jie Fan, Jiang Xue Dong, Yan Lei Li, Zhen Guang Wang, Yan Qing Chang, Li Fang Ren, Ming Su, Li Guan, Shi Gang Shen, Zhong Feng Gao, Qin Wei, Fan Xia
Summary: In this study, a d-penicillamine@Ag/Cu nanocluster (DPA@Ag/Cu NCs)-based fluorescence assay was developed to screen organophosphate pesticides (OOPs). The fluorescence intensity of DPA@Ag/Cu NCs was reduced when thiocholine produced by acetylcholinesterase (AChE) enzymatic splitting of acetylthiocholine chloride reduces the transmission of electrons. OOPs act as AChE inhibitors and retain high fluorescence while SOPs show low fluorescence intensity due to weaker toxicity to AChE. DPA@Ag/Cu NCs can be used as a fluorescent nanoneuron to construct Boolean logic tree and complex logic circuit for molecular computing.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yujuan Qiao, Jing-Jing Hu, Yuxin Hu, Chong Duan, Wenlian Jiang, Qun Ma, Yuning Hong, Wei Hua Huang, Fan Xia, Xiaoding Lou
Summary: We present a platform for detecting unfolded proteins using electrical and optical signals in a nanochannel array with outer-surface probes. The platform can differentiate folded and unfolded protein states based on the exposure of a single cysteine thiol group, allowing the distinction between normal cells and Huntington's disease mutant cells. This study provides an effective approach for analyzing proteins with distinct conformations and holds promise for facilitating the diagnoses of protein conformation-related diseases.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Physical
Dagui Wang, Jinglong Yang, Junchang Guo, Zhijuan Duan, Deihui Wang, Fan Xia, Fei Deng, Xu Deng
Summary: Liquid-like polymer lubricating surfaces (LPLSs) are solid substrates with highly flexible polymer chains grafted via covalent bonds, which enables them to have ultralow contact-angle hysteresis, repellency of various liquids and bulk ice, and stability. LPLSs have potential applications in liquid motion, biological detection, and environmental protection.
Review
Infectious Diseases
Ruvimbo Dephine Mishi, Michael Andrew Stokes, Craig Anthony Campbell, Kevin William Plaxco, Sophie Lena Stocker
Summary: Therapeutic drug monitoring (TDM) is important for optimizing drug exposure in critically ill patients. This paper explores the potential of biosensors as a solution to current challenges in measuring plasma drug concentrations and discusses their application in TDM of antibiotics. The authors argue that biosensors hold great promise for enhancing drug exposure optimization in critically ill patients.
Review
Chemistry, Multidisciplinary
Yu Huang, Lingxiao Liu, Cihui Luo, Wei Liu, Xiaoding Lou, Lei Jiang, Fan Xia
Summary: Bio-markers, such as ions, small molecules, nucleic acids, peptides, proteins, and cells, are important in living organisms and biological processes. Accurate detection of these bio-markers is significant for studying their functions, molecular diagnosis, and understanding life processes. Solid-state nanochannel-based sensing systems have shown promise in detecting bio-markers, especially with the use of outer-surface focused nanochannels. These nanochannels allow for in situ detection of large analytes and ultra-high sensitivity detection with a clear mechanism.
CHEMICAL SOCIETY REVIEWS
(2023)
