Article
Environmental Sciences
Zaiming Han, Taifeng Jiang, Liping Xie, Rongqing Zhang
Summary: The study found that high abundance of microplastics in the ocean negatively affects the formation of shells and pearls, while low-quality round pearls contain less microplastics. During the biomineralization process of bivalves, microplastics become embedded in shells and increase the expression of biomineralization-related genes.
ENVIRONMENTAL POLLUTION
(2022)
Article
Fisheries
Hua Zhang, Gaoyou Yao, Maoxian He
Summary: In this study, a novel long noncoding RNA (lncRNA7467) was identified from the pearl oyster. The lncRNA showed the highest expression in mantle tissue and was involved in the biomineralization process.
AQUACULTURE REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Yinghui Ji, Xue Yang, Dong Yang, Rongqing Zhang
Summary: Biomineralization is a widespread biological process involved in the formation of shells, teeth, and bones. The study identified a unique shell matrix protein, PU14, in Pinctada fucata, and demonstrated its function in the prismatic layer during shell formation. Expression in mantle tissue and impact on calcite crystal morphology were observed, providing new insights into matrix protein functions in shell formation.
MARINE BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Bowen Shuai, Tingyue Deng, Liping Xie, Rongqing Zhang
Summary: This study systematically investigated the regulatory effect of the NF-KB signaling pathway on biomineralization and shell regeneration in Pinctada fucata. The transcription factor Pf-Rel was found to play a positive regulatory role in the growth of the prismatic and nacreous layers, while the inhibitory protein poI-KB prevented excessive growth and accumulation. RNA-seq analysis identified potential regulatory genes controlled by Pf-Rel. Shell damage repair experiments revealed different functions of the NF-KB signaling pathway at different times. This study provides a macroscopic perspective based on transcription factors for studying biomineralization and shell regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Xin Zhang, Zehui Yin, Zhuojun Ma, Jian Liang, Zhen Zhang, Liping Yao, Xia Chen, Xiaojun Liu, Rongqing Zhang
Summary: A potential DING protein N38 was isolated and purified from the shell of Pinctada fucata, showing a mutation from the typical DINGGG- structure. It was found to induce vaterite formation during calcium carbonate crystallization, indicating its potential role in shell crystal growth control and providing new insights into biomineralization mechanisms and DING protein family studies.
MARINE BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xinwei Xiong, Chuyi Li, Zhe Zheng, Xiaodong Du
Summary: PmC1qDC-1 gene is widely expressed in various tissues of pearl oysters and plays a crucial role in shell formation and immune response.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yuting Zhang, Yu Jiao, Yiping Li, Qunli Tian, Xiaodong Du, Yuewen Deng
Summary: This study identified and analyzed miRNA molecules in mantle tissues of Pinctada fucata martensii, shedding light on the regulatory mechanisms involved in shell formation. Over-expression of specific miRNAs led to disordered growth of nacre, providing insights into the potential targets of these miRNAs in shell formation.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Environmental Sciences
Caixia Cai, Qi He, Bingyi Xie, Zhijie Xu, Cheng Wang, Chuangye Yang, Yongshan Liao, Zhe Zheng
Summary: Marine environmental change has a direct impact on the growth and survival of bivalves. This study discovered a mantle-specific long non-coding RNA (LncMPEG1) in a pearl oyster, which is highly expressed during critical periods of shell development. The expression of LncMPEG1 was found to be stimulated by shell damage, alien invasion, temperature stress, and hypoxia stress. RNA interference experiments showed that decreased expression of LncMPEG1 caused irregular growth of crystals on the inner surface of the shell. These findings suggest that LncMPEG1 may play a key regulatory role in biomineralization and response to environmental stress in the mantle.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Min Yang, Xin lei Li, Yu ting Zhang, Yue wen Deng, Yu Jiao
Summary: In this study, the precursor sequence of Pm-miR-10a-3p was obtained in the pearl oyster Pinctada fucata martensii and its expression level in the mantle tissues was detected. Pm-nAChRs alpha and Pm-NPY were identified as potential target genes of Pm-miR-10a-3p. Over-expression of Pm-miR-10a-3p downregulated the target genes and disrupted the nacre microstructure.
MARINE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jingru Yang, Zhengyi Fu, Zhenhua Ma, Gang Yu
Summary: The combined effects of temperature and salinity on the digestion and respiration metabolism of Pinctada fucata were evaluated in this study. The results showed that both temperature and salinity had significant effects on the enzymes involved in digestion and respiration. The study also found that temperature and salinity affected the activity of certain enzymes differently. These findings provide important theoretical references for shellfish culture and the establishment of related index models.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Zhe Zheng, Ruijuan Hao, Chuangye Yang, Yu Jiao, Qingheng Wang, Ronglian Huang, Yongshan Liao, Jianbo Jian, Yao Ming, Lixin Yin, Weiming He, Ziman Wang, Chuyi Li, Qi He, Kun Chen, Yuewen Deng, Xiaodong Du
Summary: Biomineralization-controlled growth of exo-/endoskeleton contributes to body growth and size diversity. This study used pearl oysters to investigate the genetic regulation of biomineralization-controlled growth and ectopic biomineralization. The insulin-like growth factor (IGF) endocrine signal and ancient single-copy transcription factors formed the regulatory network. Cross-phylum regulation of key long noncoding RNA (lncRNA) in bivalves and mammals suggests conserved genetic and epigenetic regulation. Thyroid hormone signal and apoptosis regulation affected ectopic biomineralization in pearl oysters.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Microscopy
Hiroyuki Kintsu, Alberto Perez-Huerta, Shigeru Ohtsuka, Taiga Okumura, Shinsuke Ifuku, Koji Nagata, Toshihiro Kogure, Michio Suzuki
Summary: "Mollusk shells contain unique microstructures formed by organic matrices that control calcium carbonate crystal growth. Chitin and chitinolytic enzymes are identified components of the intracrystalline organic matrix, working cooperatively to regulate crystal growth and mechanical properties. Experimental results show changes in crystal orientation and mechanical properties when chitinase inhibitor is used, indicating the importance of chitinolytic enzymes in mollusk shell formation."
Article
Environmental Sciences
Jingru Yang, Jinling Yang, Mingqiang Chen, Zhengyi Fu, Jing Sun, Gang Yu, Aimin Wang, Zhenhua Ma, Zhifeng Gu
Summary: This study investigated the physiological and biochemical changes in black and red shell Pinctada fucata under acute high and low salt stress. The results showed that red P. fucata responded more quickly to changes in salinity and exhibited reduced damage compared to black P. fucata. Additionally, P. fucata was found to be more adaptable to acute low salt environments. These findings have important implications for the selective breeding of P. fucata.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xinwei Xiong, Yanfei Cao, Zhixin Li, Ronglian Huang, Xiaodong Du, Zhe Zheng
Summary: Ecdysone is identified from the serum of pearl oyster and its receptor sequences are obtained, showing high expression in early embryos and biomineralized mantle tissue. Ecdysone signaling plays important roles in shell repair by inducing expression of growth factors, transcription factors, and shell matrix proteins, although the receptor proteins do not form dimers in bivalves. These findings provide insights into the regulation pathway and function of ecdysone in bivalve species.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Kei Futagawa, Taichi Morioka, Kazuo Furihata, Hiroyuki Watanabe, Yutaka Ito, Teppei Ikeya, Akiko Hokura, Koji Nagata, Michio Suzuki
Summary: Shellfish and crustaceans use calcium carbonate (CaCO3) as a protective shell and exoskeleton. In this study, the interaction between the low-complexity region and CaCO3 was analyzed to elucidate the microstructure formation mechanism. The results showed that the Pif 80 acidic region binds the aragonite crystal surface via Asp side chains with basic residues, providing new insights into the function of acidic and basic amino acids in biomineral formation.
CRYSTAL GROWTH & DESIGN
(2023)
Article
Environmental Sciences
Wei Liu, He Wang, Zhenzhu Xi, Rongqing Zhang, Xiaodi Huang
Summary: This paper introduces a physics-driven deep learning method for geophysical inversion, which incorporates physics laws into network training and takes advantage of both data-driven and traditional physics-based methods to handle complex realistic exploration scenarios.
Article
Engineering, Electrical & Electronic
Ang Ji, Xu Guo, Rongqing Zhang, Jianjun Wu, Xiang Cheng
Summary: This paper studies the deployment of a high-altitude platform (HAP) as a supplement for terrestrial networks in the fifth-generation (5G) network. It focuses on serving both conventional terrestrial users (TUs) and unmanned aerial vehicles (UAVs) simultaneously. The paper addresses the challenges of HAP deployment and complex channel and resource allocation.
IET COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Bowen Shuai, Tingyue Deng, Liping Xie, Rongqing Zhang
Summary: In this study, a novel matrix protein named PNU5 was discovered to play a regulatory role in both prismatic layer and nacreous layer formation in Pinctada fucata. Functional studies showed that PNU5 might be involved in shell formation in a positive manner. Repression of pnu5 affected the structure of both layers, while the recombinant protein rPNU5 enhanced the precipitation rate of CaCO3 and altered the morphology of the crystals.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Li Yi, Bing Zou, Liping Xie, Rongqing Zhang
Summary: This article investigates a novel vaterite stabilizer acidic matrix protein PNU7, which is found in both the prism and nacre of Pinctada fucata. The study shows that PNU7 can trigger the formation of stable vaterite even with a deficiency in Mg2+ and low Ca2+ levels. Moreover, increasing PNU7 in the calcium carbonate crystallization system leads to a decrease in crystal size and an increase in nucleation quantity. The results suggest that PNU7 not only plays a role in shell formation but also regulates the formation of nacre through surface energy minimization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Geochemistry & Geophysics
Guozhi Xie, Haibo Yan, Guangming Li, Jia Guo, Fang Liu, Qian Chen, Rongqing Zhang, Lipeng Zhang
Summary: This study focused on the in-situ major and trace element and boron isotope compositions of tourmalines from two-mica granites in the Cuona area. The results indicated that tourmalines in the Cuonadong leucogranite crystallized earlier than those in the Cuona leucogranite during magmatic differentiation. The volatile contents of tourmaline were genetically related to incompatible elements, which led to tourmaline zonation and the enrichment of Sr, Zn, and Pb. The B isotopic composition of tourmaline suggested that the granitic magma was dominantly derived from the partial melting of the metasedimentary source rocks, and their B isotope variations were likely due to fluid exsolution during B-rich melt evolution. High rare metal contents in tourmalines indicated great mineralization potential in the two-mica granites of the Cuona area.
Article
Engineering, Chemical
Rongqing Zhang, Haifeng Chen, Qiang Yu, Yu Zhang, Fen Liu, Fan Wang, Xiaoyan Chen, Yunyun Liu
Summary: The recycling of bioactive compounds from Chinese herb residue is hindered by low extraction efficiency and high energy environmental impact. A microwave-assisted deep eutectic solvent (DES) extraction method was developed to enhance the extraction yield of bioactive compounds from cinnamon twigs residue. The optimal DES system of choline chloride/ethylene glycol achieved a significant increase in extraction yield, with a 41.528% and 41.443% improvement for cinnamic acid and cinnamic aldehyde, respectively, compared to traditional extraction processes. The extraction process was found to be a spontaneous, exothermic, and irreversible reaction, following a second-order kinetic model. Quantum chemistry analysis revealed that the extraction capacity relied mainly on hydrogen bonding force, and the difference in binding energy between the solvent and the target compounds explained the variation in extraction yields.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Engineering, Electrical & Electronic
Xinge Li, Xiaoya Hu, Rongqing Zhang, Chunjie Zhou, Quan Yin, Liuqing Yang
Summary: This paper proposes a 5G network security analysis approach combining formal modeling and attack penetration to reveal the impact of network threats on 5G-based industrial systems. The approach establishes 5G network models based on topology and transmission events to handle diverse and hidden attack routes and behaviors. It integrates an attack module into the network model to exploit and quantify potential vulnerabilities. Simulation results identify and quantify network vulnerabilities under various attacks, and a threshold of access probability is found to measure the impacts of attacks on network reliability. The feasibility of the approach is further proven through a practical case study.
IEEE TRANSACTIONS ON WIRELESS COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jingliang Huang, Yangjia Liu, Taifeng Jiang, Wentao Dong, Liping Xie, Rongqing Zhang
Summary: Molluscs rapidly repair damaged shells to ensure survival, but the control mechanism of this process is unclear. Using scanning electron microscope and histochemical analysis, this study examined shell regeneration in Pinctada fucata. Damage to the shell caused the mantle tissue to retract, resulting in the rearrangement of mantle zones and the alteration of shell morphology. The up-regulation of shell matrix protein genes and the change in CaCO3 dynamics promoted shell formation. This study reveals the relationship between mantle tissue activities and regenerated shell morphology.
JOURNAL OF STRUCTURAL BIOLOGY
(2023)
Review
Plant Sciences
Ning Yang, Qingyang Zhang, Jingyun Chen, Shilin Wu, Ran Chen, Liping Yao, Bailei Li, Xiaojun Liu, Rongqing Zhang, Zhen Zhang
Summary: Natural antioxidants, such as microalgal bioactive components, are preferred due to their non-toxic and effective properties. This study analyzed antioxidant research on microalgae-based bioactive compounds using a bibliometric approach. The findings highlight the most studied areas, including effectiveness, antioxidant mechanism, and applications in food, cosmetics, and medicine. Future trends focus on improving extraction rates and stability through altering the microalgae culture environment or incorporating chemicals like nanoparticles.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Computer Science, Information Systems
Zhiwei Wei, Bing Li, Rongqing Zhang, Xiang Cheng
Summary: This paper proposes an integrated charging and computing (IC2) architecture, which combines vehicular fog computing (VFC) and smart EV charging for optimization of electric vehicle (EV) charging and computing tasks in a smart grid. A multiattribute contract-based charging protocol is provided using contract theory, and a convex-concave-procedure-based contract optimization algorithm is designed. A heuristic offloading algorithm is also proposed for task assignment to heterogeneous EVs. Numerical results show that the proposed multiattribute contract-based charging-computing scheme effectively benefits both the charging stations and EVs, while improving task computation capability in the EV-integrated smart grid.
IEEE INTERNET OF THINGS JOURNAL
(2023)
Article
Geosciences, Multidisciplinary
Guozhi Xie, Haibo Yan, Rongqing Zhang, Kun Wang, Lipeng Zhang
Summary: This study examines two types of hydrothermal tourmaline (Tur-1 and Tur-2) and concludes that Tur-1 originated from a magmatic-hydrothermal fluid with little influence from surrounding rocks, whereas Tur-2 in the skarn involved more fluid from surrounding rocks. The findings have important implications for understanding the formation and ore-forming mechanisms of leucogranite.
FRONTIERS IN EARTH SCIENCE
(2023)
Article
Biology
Zhonghui Xia, Xin Zhang, Yujuan Zhou, Liping Yao, Zhen Zhang, Rongqing Zhang, Xiaojun Liu
Summary: In this study, a novel matrix protein cysrichin was cloned and shown to induce vaterite crystallization. Cysrichin plays an important role in shell formation of freshwater mussels, providing new insights into mineralization in these organisms.
Article
Computer Science, Information Systems
Zhiwei Wei, Bing Li, Rongqing Zhang, Xiang Cheng, Liuqing Yang
Summary: This article proposes a novel task-block-based offloading paradigm for vehicular fog computing, which reduces computation latency and solves the complex offloading problem through the overlapping matching coalition scheme.
IEEE INTERNET OF THINGS JOURNAL
(2023)
Proceedings Paper
Computer Science, Hardware & Architecture
Lingyun Zhou, Wenqiang Pu, Ming-yi You, Rongqing Zhang, Qingjiang Shi
Summary: In this paper, the problem of cooperative sensing via a system of multi-unmanned aerial vehicles (UAVs) is considered. Each UAV is equipped with a directional antenna to perform detection tasks for multiple targets. The detection probability is chosen as the metric to measure the system's perception ability, and the goal is to maximize the sum detection probability by optimizing UAVs' deployment and directional antenna orientations. To tackle the nonconvexity of the problem, it is decomposed into two sub-problems and efficient algorithms are developed using block coordinate descent and Gibbs Sampling approaches.
2023 IEEE WIRELESS COMMUNICATIONS AND NETWORKING CONFERENCE, WCNC
(2023)
Article
Computer Science, Information Systems
Zhiwei Wei, Bing Li, Rongqing Zhang, Xiang Cheng, Liuqing Yang
Summary: This article proposes a cooperative vehicular fog computing architecture called OCVC, which fully utilizes the potential resources of vehicular fog nodes to address the overload issue in computation-intensive tasks. Unlike traditional architectures, OCVC enables vehicles to participate in multiple computing groups simultaneously, utilizing resources in an overlapping manner. The article also presents a distributed OCVC scheme that employs the overlapping coalition formation game framework and a heuristic offloading algorithm to solve complex group formation, resource allocation, and task assignment problems, with simulation results showing better performance compared to other benchmarks.
IEEE TRANSACTIONS ON MOBILE COMPUTING
(2023)