Journal
CHANNELS
Volume 8, Issue 3, Pages -Publisher
LANDES BIOSCIENCE
DOI: 10.4161/chan.28146
Keywords
sodium channel; patch clamp; exon skipping; protein kinase A; pain
Categories
Funding
- Deutsche Forschungsgemeinschaft [SFB 604]
- Landesgraduiertenprogramm Thuringen
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The voltage-gated sodium channel Na(V)1.8 (encoded by SCN10A) is predominantly expressed in dorsal root ganglia (DRG) and plays a critical role in pain perception. We analyzed SCN10A transcripts isolated from human DRGs using deep sequencing and found a novel splice variant lacking exon 11, which codes for 98 amino acids of the domain I/II linker. Quantitative PCR analysis revealed an abundance of this variant of up to 5-10% in human, while no such variants were detected in mouse or rat. Since no obvious functional differences between channels with and without the exon-11 sequence were detected, it is suggested that SCN10A exon 11 skipping in humans is a tolerated event.
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