Journal
CELLULAR SIGNALLING
Volume 23, Issue 5, Pages 911-919Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2011.01.019
Keywords
Cdk2; SHP-1; Endocytosis; Insulin receptor
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada [OGPO157551]
- Canadian Diabetes Association
- Foundation of Stars
- Canadian Institutes of Health Research
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The cyclin-dependant kinase Cdk2 is compartmentalized in endosomes but its role is poorly understood. Here we show that Cdk2 present in hepatic endosome fractions is strictly located in a Triton X-100-resistant environment. The endosomal Cdk2 was found to be associated with the protein tyrosine phosphatase SHP-1, a regulator of insulin clearance, and the actin anchor beta-catenin, a known substrate for both Cdk2 and SHP-1. In the plasma membranes and endosome fractions, beta-catenin is associated with CEACAM1, also known as regulator of insulin clearance. We show that beta-catenin, not CEACAM1, is a substrate for Cdk2. Partial down-modulation of Cdk2 in HEK293 cells increased the rate of insulin internalization. These findings reveal that Cdk2 functions, at least in part, via a Cdk2/SHP-1/beta-catenin/CEACAM1 axis, and show for the first time that Cdk2 has the capacity to regulate insulin internalization. (C) 2011 Elsevier Inc. All rights reserved.
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