Article
Neurosciences
Devanjan Dey, Vadanya Shrivastava, Diksha Joshi, Chitra Mohinder Singh Singal, Sagar Tyagi, Muzaffer Ahmed Bhat, Paritosh Jaiswal, Jai Bhagwan Sharma, Jayanth Kumar Palanichamy, Subrata Sinha, Pankaj Seth, Sudip Sen
Summary: This study found that human fetal neural stem cells tend to differentiate into neuronal cells under low oxygen conditions, and the WNT signaling pathway may be involved in this process.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Victor Baeza, Manuel Cifuentes, Fernando Martinez, Eder Ramirez, Francisco Nualart, Luciano Ferrada, Maria Jose Oviedo, Isabelle De Lima, Ninoschka Troncoso, Natalia Saldivia, Katterine Salazar
Summary: Ependymal cells with multiple apical cilia are important for brain function, and the protein IIIG9 plays a crucial role in maintaining their adherens junctions. Loss of ependymal cell polarity can lead to tumor formation, particularly in children, and understanding the function of IIIG9 may provide insights into potential therapies for conditions such as hydrocephalus or neoplastic transformation.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Sophia Shaka, Nicholas Carpo, Victoria Tran, Araceli Espinosa-Jeffrey
Summary: Human neural stem cells flown into space were successfully induced into astrocytes after a slow process lasting several weeks, showing slow but significant proliferation and a tendency to continue proliferating at the same pace. Astrocytes, a major type of glial cells, are crucial for the normal function of the central nervous system and are increasingly recognized as a critical component in most neurodegenerative diseases. Understanding the effects of space microgravity on astrocytes is essential for long-duration space travel.
APPLIED SCIENCES-BASEL
(2021)
Article
Cell Biology
Gyu-Bum Yeon, Won-Ho Shin, Seo Hyun Yoo, Dongyun Kim, Byeong-Min Jeon, Won-Ung Park, Yeonju Bae, Jae-Yong Park, Seungkwon You, Dokyun Na, Dae-Sung Kim
Summary: The study presents an efficient and rapid method for generating physiologically functional astrocytes from human pluripotent stem cells. With RNA sequencing and functional analyses, it shows progressive transcriptomic and physiological changes in the cells, resembling in vivo astrocyte development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Physiology
Chia-Yu Yeh, Kuan-Yu Wu, Guo-Jen Huang, Alexei Verkhratsky
Summary: Adult neurogenesis is an important process that contributes to adaptive network remodelling in the brain. This review focuses on the cellular characteristics and roles of radial astrocytes (RSA) and parenchymal astrocytes in adult neurogenesis in different brain regions. The potential therapeutic applications of RSA and astrocytes in cell replacement and regeneration strategies are also discussed.
Article
Oncology
Pan Wang, Sheng Gong, Bin Liao, Jinyu Pan, Junwei Wang, Dewei Zou, Lu Zhao, Shuanglong Xiong, Yangmin Deng, Qian Yan, Nan Wu
Summary: The study demonstrated that in a hypoxic microenvironment, the HIF1 alpha/HIF2 alpha-Sox2 network induced the formation of glioma stem cells through the dedifferentiation of differentiated glioma cells, thus promoting glioma cell chemoresistance. Both HIF1 alpha and HIF2 alpha, as genes upstream of Sox2, regulate the malignant progression of glioma through dedifferentiation. The knockout of HIF1 alpha, HIF2 alpha, and Sox2 in glioma cells under hypoxic conditions led to decreased CD133 and CD15 expression and inhibited sphere formation.
Article
Virology
Michele Bengue, Pauline Ferraris, Jonathan Barthelemy, Cheikh Tidiane Diagne, Rodolphe Hamel, Florian Liegeois, Antoine Nougairede, Xavier de Lamballerie, Yannick Simonin, Julien Pompon, Sara Salinas, Dorothee Misse
Summary: The study indicates that MAYV and CHIKV induce different antiviral responses in astrocytes, with MAYV being more susceptible to the antiviral effects of interferons.
Article
Multidisciplinary Sciences
Carlos Sebastian, Christina Ferrer, Maria Serra, Jee-Eun Choi, Nadia Ducano, Alessia Mira, Manasvi S. Shah, Sylwia A. Stopka, Andrew J. Perciaccante, Claudio Isella, Daniel Moya-Rull, Marianela Vara-Messler, Silvia Giordano, Elena Maldi, Niyati Desai, Diane E. Capen, Enzo Medico, Murat Cetinbas, Ruslan Sadreyev, Dennis Brown, Miguel N. Rivera, Anna Sapino, David T. Breault, Nathalie Y. R. Agar, Raul Mostoslavsky
Summary: This study reveals that the histone deacetylase SIRT6 controls tumor initiation in intestinal cancer through regulating glucose metabolism. The loss of SIRT6 leads to an increase in the number of intestinal stem cells (ISCs), resulting in enhanced tumor initiating potential. The researchers found a metabolic compartmentalization within the intestinal epithelium and adenomas, where a rare population of cells exhibit features of Warburg-like metabolism characterized by high pyruvate dehydrogenase kinase (PDK) activity. These cells are quiescent cells expressing +4 ISCs and enteroendocrine markers, and their active glycolysis suppresses ROS accumulation and enhances their stem cell and tumorigenic potential.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Carlos Bueno, Miguel Blanquer, David Garcia-Bernal, Salvador Martinez, Jose M. Moraleda
Summary: This study demonstrates that human bone marrow-derived cells can rapidly adopt a neural-like morphology and can form binucleated cells independently of cell fusion events. The study also reveals that the neural-like differentiation of these cells involves the formation of intermediate cells, which can redifferentiate into neural-like cells, mesenchymal cells, or switch lineages without cell division.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Alexandra Poetzsch, Sara Zocher, Stefanie N. Bernas, Odette Leiter, Annette E. Ruenker, Gerd Kempermann
Summary: The study showed that L-lactate has a pro-proliferative effect on neural precursor cells, which is dependent on the active lactate transport by monocarboxylate transporters and is not linked to amplified mitochondrial respiration. L-lactate redirected glucose metabolism to the pentose phosphate pathway, as indicated by increased glucose-6-phosphate dehydrogenase activity and decreased glycolysis.
Article
Engineering, Biomedical
Yi-Chen Ethan Li, Yasamin A. Jodat, Roya Samanipour, Giulio Zorzi, Kai Zhu, Minoru Hirano, Karen Chang, Adnan Arnaout, Shabir Hassan, Navneet Matharu, Ali Khademhosseini, Mina Hoorfar, Su Ryon Shin
Summary: Researchers have used embedded 3D bioprinting to create structured brain-like co-culture constructs with neurospheroid patterns, mimicking a supportive bed resembling a neural stem cell growth environment. These brain-like co-cultures can provide a reproducible platform for modeling neurological diseases, neural regeneration, and drug development and repurposing.
Article
Multidisciplinary Sciences
Ioannis Sarropoulos, Mari Sepp, Robert Fromel, Kevin Leiss, Nils Trost, Evgeny Leushkin, Konstantin Okonechnikov, Piyush Joshi, Peter Giere, Lena M. Kutscher, Margarida Cardoso-Moreira, Stefan M. Pfister, Henrik Kaessmann
Summary: This study investigated the regulatory basis of mouse cerebellum development from early neurogenesis to adulthood using snATAC-seq profiles. The research revealed spatiotemporal heterogeneity among progenitor cells and gradual divergence in the regulatory programs of cerebellar neurons during differentiation. Comparison to vertebrate genomes showed a shared decrease in CRE conservation during development and differentiation, as well as differences in constraint between cell types. The study provides insights into mammalian organ development dynamics and evolutionary gene regulation in cerebellar cells.
Article
Cell & Tissue Engineering
Anastasiia D. Kurenkova, Lei Li, Anna P. Usanova, Xiaogang Feng, Baoyi Zhou, Andrei A. Nedorubov, Alexey Lychagin, Andrei S. Chagin
Summary: Articular cartilage has limited ability to self-repair, and clinical approaches to cartilage regeneration are necessary. The current method involves expansion of autologous chondrocytes followed by reimplantation, but the outcome is often unsatisfactory due to dedifferentiation. In recent years, articular stem/progenitor cells (artSPCs) have been discovered, which have the potential to repair damaged cartilage. This study found that modulation of the Notch pathway during expansion facilitated chondrogenic potential of artSPCs and chondrocytes, while activation of the pathway abrogated this potential.
Article
Gastroenterology & Hepatology
Shijie He, Peng Lei, Wenying Kang, Priscilla Cheung, Tao Xu, Miyeko Mana, Chan Young Park, Hongyan Wang, Shinya Imada, Jacquelyn O. Russell, Jianxun Wang, Ruizhi Wang, Ziheng Zhou, Kashish Chetal, Eric Stas, Vidisha Mohad, Peter Bruun-Rasmussen, Ruslan I. Sadreyev, Richard A. Hodin, Yanhang Zhang, David T. Breault, Fernando D. Camargo, Omer H. Yilmaz, Jeffrey J. Fredberg, Nima Saeidi
Summary: This study investigates the impact of tissue stiffening on the fate and function of intestinal stem cells (ISCs), and finds that matrix stiffness can regulate the stemness and differentiation trajectory of ISCs, supporting the direct role of fibrosis-induced gut stiffening in epithelial remodeling in inflammatory bowel disease (IBD).
Article
Cell Biology
Meng Zhang, Ruyi Peng, Haizhou Wang, Zhenwei Yang, Hailin Zhang, Yangyang Zhang, Meng Wang, Hongling Wang, Jun Lin, Qiu Zhao, Jing Liu
Summary: This study discovered that Nanog can induce cellular dormancy in colorectal cancer cells through the FAO/ACLY-dependent pathway. This finding reveals a specific mechanism governing cellular dormancy in colorectal cancer.
CELL DEATH & DISEASE
(2022)
Article
Microbiology
Xin Zhu, Anshan Shan, Zhi Ma, Wei Xu, Jiajun Wang, Shuli Chou, Baojing Cheng
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2015)
Article
Multidisciplinary Sciences
Matthew C. Canver, Elenoe C. Smith, Falak Sher, Luca Pinello, Neville E. Sanjana, Ophir Shalem, Diane D. Chen, Patrick G. Schupp, Divya S. Vinjamur, Sara P. Garcia, Sidinh Luc, Ryo Kurita, Yukio Nakamura, Yuko Fujiwara, Takahiro Maeda, Guo-Cheng Yuan, Feng Zhang, Stuart H. Orkin, Daniel E. Bauer
Article
Hematology
Elenoe C. Smith, Sidinh Luc, Donyell M. Croney, Mollie B. Woodworth, Luciano C. Greig, Yuko Fujiwara, Minh Nguyen, Falak Sher, Jeffrey D. Macklis, Daniel E. Bauer, Stuart H. Orkin
Article
Multidisciplinary Sciences
Takeshi Masuda, Xin Wang, Manami Maeda, Matthew C. Canver, Falak Sher, Alister P. W. Funnell, Chris Fisher, Maria Suciu, Gabriella E. Martyn, Laura J. Norton, Catherine Zhu, Ryo Kurita, Yukio Nakamura, Jian Xu, Douglas R. Higgs, Merlin Crossley, Daniel E. Bauer, Stuart H. Orkin, Peter V. Kharchenko, Takahiro Maeda
Article
Medicine, Research & Experimental
Samuel Lessard, Emily Stern Gatof, Melissa Beaudoin, Patrick G. Schupp, Falak Sher, Adnan Ali, Sukhpal Prehar, Ryo Kurita, Yukio Nakamura, Esther Baena, Jonathan Ledoux, Delvac Oceandy, Daniel E. Bauer, Guillaume Lettre
JOURNAL OF CLINICAL INVESTIGATION
(2017)
Article
Biochemistry & Molecular Biology
Nan Liu, Victoria V. Hargreaves, Qian Zhu, Jesse V. Kurland, Jiyoung Hong, Woojin Kim, Falak Sher, Claudio Macias-Trevino, Julia M. Rogers, Ryo Kurita, Yukio Nakamura, Guo-Cheng Yuan, Daniel E. Bauer, Jian Xu, Martha L. Bulyk, Stuart H. Orkin
Article
Neurosciences
Andra M. Smith, Claude Messier
FRONTIERS IN HUMAN NEUROSCIENCE
(2014)
Article
Neurosciences
Reno M. Gandhi, Cary S. Kogan, Claude Messier, Lindsey S. MacLeod
Review
Neurosciences
J. J. Boulanger, C. Messier
Article
Health Care Sciences & Services
Delyana Ivanova Miller, France Aube, Vincent Talbot, Michele Gagnon, Claude Messier
TELEMEDICINE AND E-HEALTH
(2014)
Letter
Biochemical Research Methods
Jonathan Y. Hsu, Charles P. Fulco, Mitchel A. Cole, Matthew C. Canver, Danilo Pellin, Falak Sher, Rick Farouni, Kendell Clement, Jimmy A. Guo, Luca Biasco, Stuart H. Orkin, Jesse M. Engreitz, Eric S. Lander, J. Keith Joung, Daniel E. Bauer, Luca Pinello
Article
Genetics & Heredity
Falak Sher, Mir Hossain, Davide Seruggia, Vivien A. C. Schoonenberg, Qiuming Yao, Paolo Cifani, Laura M. K. Dassama, Mitchel A. Cole, Chunyan Ren, Divya S. Vinjamur, Claudio Macias-Trevino, Kevin Luk, Connor McGuckin, Patrick G. Schupp, Matthew C. Canver, Ryo Kurita, Yukio Nakamura, Yuko Fujiwara, Scot A. Wolfe, Luca Pinello, Takahiro Maeda, Alex Kentsis, Stuart H. Orkin, Daniel E. Bauer
Article
Neurosciences
Alexandria Beland-Millar, Masaki Takimoto, Taku Hamada, Claude Messier
Article
Biotechnology & Applied Microbiology
Vivien A. C. Schoonenberg, Mitchel A. Cole, Qiuming Yao, Claudio Macias-Trevino, Falak Sher, Patrick G. Schupp, Matthew C. Canver, Takahiro Maeda, Luca Pinello, Daniel E. Bauer
Article
Behavioral Sciences
Jenna J. Boulanger, Claude Messier
BEHAVIOURAL BRAIN RESEARCH
(2017)