Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 33, Issue 5, Pages 1568-1578Publisher
KARGER
DOI: 10.1159/000358720
Keywords
Hepatocyte; SREBP-1c; Fat deposit
Categories
Funding
- Program for New Century Excellent Talents in University [NCET-11-0199]
- National Key Technology RD Program [2012BAD12B03]
- National Natural Science Foundation of China (Beijing, China) [30871897, 30972224, 31072178, 31172372, 31172172, 31201961, 31272621]
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Background: Fatty liver is a major metabolic disorder that occurs during early lactation in high-producing dairy cows. Sterol regulatory element-binding protein-1c (SREBP-1c) is an important transcription factor that regulates lipid synthesis by regulating the expression of lipid metabolism genes. Methods: In this study, we reduced the expression of SREBP-1c by adenovirus-mediated SREBP-1c with a low expression vector (AD-GFP-SREBP-1c) to study the effects of SREBP-1c on lipid deposits in bovine hepatocytes. The expression levels and enzyme activities of SERBP-1c and its target genes were determined by real-time PCR, western blot, and ELISA. Results: These results showed that Ad-GFP-SREBP-1c could inhibit SREBP-1c expression. The expression of the lipid synthesis enzyme acetyl-CoA carboxylase (ACC) was down-regulated. The expression levels of the lipid oxidation enzymes long-chain fatty acyl-COA synthetase (ACSL-1), carnitine palmitoyltransferase. (CPT-I), carnitine palmitoyltransferase Pi (CPT-Pi), and beta-hydroxyacyl-CoA-DH (HADH) were significantly elevated. Furthermore, the expression levels of factors involved in the assembly and transport of very low-density lipoproteins (VLDLs), such as apolipoprotein B100 (ApoB), apolipoprotein E (ApoE), and microsomal triglyceride transfer protein (MTTP) were decreased comparison with the negative control and the blank control groups, but the low-density lipoprotein receptor (LDLR) was elevated. The concentrations of TG (triglyceride) and VLDL were also reduced. Conclusion: These data suggest that low SREBP-1c expression can decrease lipid synthesis, increase lipid oxidation, and decrease the TG and VLDL content in bovine hepatocytes. Copyright (C) 2014 S. Karger AG, Basel
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