Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 27, Issue 2, Pages 121-128Publisher
KARGER
DOI: 10.1159/000325214
Keywords
Adenine nucleotide translocase; Contraction-relaxation cycle; Heart; Cardiomyocytes; Myofibrillar proteins; Transgenic rat
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG) [Sonderforschungsbereich Transregio 19, C7, C1]
- Research Grant of the University Medical Center Giessen and Marburg
Ask authors/readers for more resources
Background/Aims: The adenine nucleotide translocase (ANT) exchanges ATP and ADP over the inner mitochondrial membrane, supplying the cells with energy. Interestingly, myocardial ANTI overexpression preserves cardiac structure and function under pathophysiological conditions. To ascertain whether the contractile system is directly affected by increased ANTI expression, we analyzed cell morphology, contraction and relaxation parameters of ANTI transgenic (ANT1-TG) cardiomyocytes, myofibrillar protein expression, and Ca2+ handling in ANT1-TG rat hearts. Results: ANT1-TG cardiomyoycytes displayed an elevation in cell volume (52.6 +/- 12.0%; p<0.0001) in comparison to wildtype (WT) cells. Concurrently, contractile function in ANT1-TG cells was significantly increased, measured by a decline in time to peak contraction (UP) and RT50, the time from peak contraction to 50% relaxation, during stimulation with 0.5, 1, and 2 Hz. Quantification of myofibrillar proteins exhibited a marked increase in total cardiac myosin heavy chain (51.8 +/- 12.8%) (p<0.03), beta myosin heavy chain (22.9 +/- 5.0%; p<0.03), actin (23.8 +/- 8.8%; p<0.05), and troponin I (51.5 +/- 13.7%; p<0.01). Regarding intracellular Ca2+ handling, ANT1-TGs revealed a significant elevation in sarcoplasmic reticulum (SR) Ca2+ ATPase (SERCA2a) protein level (22.2 +/- 4.7%; p<0.01) associated with increased Ca2+ uptake into the SR (34%; p<0.01). Moreover, the plasmalemmal Ca2+ ATPase (PMCA) indicated advanced protein expression (23.8 +/- 4.8%; p<0.01), whereas the protein amount of the Na+/Ca2+ exchanger was not altered in ANTI overexpressing hearts. Conclusion: These data reveal a close association of elevated mitochondria! ATP/ADP transportation via ANTI with increased contractile function. Furthermore, the ANT1-TGs exhibit an elevation in SR Ca2+ transport that contributes to increased cardiac work, which may protect the heart under pathophysiological conditions. Copyright (C) 2011 S Karger AG, Basel
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available