Article
Biology
Dorottya Nagy, Clare M. C. Gillis, Katie Davies, Abigail L. Fowden, Paul Rees, John W. Wills, Katherine Hughes
Summary: The research focuses on the growth of sheep TDLUs as a model for breast development, revealing differences in epithelial nuclear Ki67 positivity between younger and older lambs. The study also highlights a dynamic immune microenvironment in the sheep mammary gland, indicating its value as a model system for understanding postnatal breast development.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell Biology
Jessica Elswood, Scott J. Pearson, H. Ross Payne, Rola Barhoumi, Monique Rijnkels, Weston W. Porter
Summary: Through studying the differentiation and metabolism of mammary epithelial cells, it was found that mitophagy occurs selectively during the differentiation process. Inhibiting autophagy impairs cell differentiation, while the PRKN protein is necessary for cell differentiation.
Article
Cell Biology
Anna Kosenko, Tomer-Meir Salame, Gilgi Fridlander, Itamar Barash
Summary: Implanting close or far bovine stromal layers as an endogenous niche for transplanted stem cells into mouse fat pads enhances stem cell growth and development, with the close stroma better supporting stem cell take rate and outgrowth. RNA-Seq analysis revealed lower complement activity in this layer, favoring epithelial outgrowth development.
CELL AND TISSUE RESEARCH
(2022)
Article
Cell Biology
Kevin Roarty
Summary: In this article, Pfannenstein and Macara demonstrate that intercalation within the terminal end bud is controlled by the tight junction organizing protein ZO-1 and dynamic actin cytoskeletal rearrangements to drive ductal elongation.
DEVELOPMENTAL CELL
(2023)
Article
Cell Biology
Amritha Varshini Hanasoge Somasundara, Matthew A. Moss, Mary J. Feigman, Chen Chen, Samantha L. Cyrill, Michael F. Ciccone, Marygrace C. Trousdell, Macy Vollbrecht, Siran Li, Jude Kendall, Semir Beyaz, John E. Wilkinson, Camila O. dos Santos
Summary: Pregnancy induces expansion of NKT-like immune cells and upregulation of immune signaling molecules in mammary epithelial cells. Lack of CD1d expression or activated NKT cells may lead to mammary oncogenesis.
Article
Agriculture, Dairy & Animal Science
Yutaka Suzuki, Sachi Chiba, Koki Nishihara, Keiichi Nakajima, Akihiko Hagino, Won-Seob Kim, Hong-Gu Lee, Tomonori Nochi, Toru Suzuki, Sang-Gun Roh
Summary: This study explored the role of chemerin in promoting bovine mammary epithelial cell growth and protecting against oxidative stress, suggesting its potential as a therapeutic target for diseases in the bovine mammary gland.
Article
Biochemistry & Molecular Biology
Lilia Sanchez, Jessica Epps, Steven Wall, Cole McQueen, Scott J. Pearson, Kelly Scribner, Elizabeth A. Wellberg, Erin D. Giles, Monique Rijnkels, Weston W. Porter
Summary: The functionally differentiated mammary gland adapts to extreme levels of stress by activating specific protective mechanisms. The breast tumor suppressor gene SIM2s is identified as a novel regulator of mitophagy, a key component of this stress response.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Karolina Kowalska, Dominika Ewa Habrowska-Gorczynska, Marta Justyna Koziel, Kinga Anna Urbanek, Kamila Dominska, Agnieszka Wanda Piastowska-Ciesielska
Summary: Mycotoxins are common food and feed pollutants, with potential toxicity. This study found that AOH significantly affects cell viability and motility, inducing oxidative stress, DNA damage, and cell cycle arrest.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pathology
Yumiko Kamiya, Tsubasa Saito, Moeko Aoki, Mizuho Takagi, Kochi Kakimoto, Yuko Yamaguchi
Summary: We present a rare case of fibroadenoma in a 7-week-old male SD rat, with rapid growth and unique histological features including island-like proliferation of epithelial cells and a mucinous mesenchymal component of fibroblasts. This case highlights the exceptional nature of fibroadenoma occurrence in a young male rat and provides insight into the histopathological characteristics of this type of tumor. (DOI: 10.1293/tox.2022-0071; J Toxicol Pathol 2023; 36: 131-138)
JOURNAL OF TOXICOLOGIC PATHOLOGY
(2023)
Article
Cell Biology
Taylor R. Rutherford, Alan M. Elder, Traci R. Lyons
Summary: The study reveals that SEMA7A promotes anoikis resistance in mammary epithelial cells and plays a role during mammary gland involution. SEMA7A is primarily expressed in cells expressing alpha 6-integrin, and a decrease in luminal progenitor cells is observed in SEMA7A-/- mice during involution.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Jingqiang Wang, Wenqian Song, Ruikai Yang, Chao Li, Ting Wu, Xiao Bing Dong, Bin Zhou, Xizhi Guo, Jianfeng Chen, Zhiyong Liu, Qing Cissy Yu, Wen Li, Junfen Fu, Yi Arial Zeng
Summary: This study reveals a crucial signaling axis in which fibroblasts relay Wnt signals from endothelial cells to organize epithelial patterning. It also demonstrates the importance of different niche components in coordinating epithelial organogenesis and tissue homeostasis.
Article
Multidisciplinary Sciences
Wen Bu, Yi Li
Summary: Mouse mammary glands are composed of ductal trees lined with epithelial cells, which are responsible for mammary gland function and tumorigenesis. Intraductal injection of a viral vector carrying genes of interest into the mouse mammary ductal tree is a critical step for evaluating gene function and generating tumor models. Lentiviral, retroviral, adenoviral, or AAV vectors can be used for this purpose. This study demonstrates the delivery of a gene of interest into mammary epithelial cells using intraductal injection of a viral vector, showing stable expression and oncogene-induced lesions and tumors.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Multidisciplinary Sciences
Wen Bu, Yi Li
Summary: Mouse mammary glands have ductal trees lined by epithelial cells with one opening at each nipple. Introducing genes into these epithelial cells is crucial for studying gene function and generating mammary tumor models. This can be done by intraductal injection of a viral vector carrying the genes. Different types of viral vectors can be used, such as lentiviruses and retroviruses. This study demonstrates the delivery of genes of interest into mammary epithelial cells using intraductal injection of a viral vector, and the subsequent expression and effects of these genes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Agriculture, Dairy & Animal Science
Hongmei Zhu, Qianqian Jia, Yanyan Zhang, Dongming Liu, Diqi Yang, Li Han, Jianguo Chen, Yi Ding
Summary: It is still unclear how ovarian hormones affect goat lactation by regulating cell-cell junctions. This study reveals that ovarian hormones can elevate the expression of cell-cell junction proteins, which may affect intercellular space and molecule transportation between goat mammary epithelial cells. The results suggest that ovarian hormones may play a role in regulating milk production by influencing the expression of cell-cell junction proteins between mammary epithelial cells.
Article
Biochemistry & Molecular Biology
Zhiyun Hao, Jiqing Wang, Yuzhu Luo, Jiang Hu, Xiu Liu, Shaobin Li, Mingna Li, Bingang Shi, Liyan Hu, Yuan Liu, Huimin Zhen
Summary: miR-200c promotes the synthesis of triglycerides in ovine MECs by targeting PANK3, and its overexpression inhibits MECs proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Hamood N. Al Kindi, Mahmoud Shehata, Ayman M. Ibrahim, Mohamed Roshdy, Walid Simry, Yasmine Aguib, Magdi H. Yacoub
ANNALS OF THORACIC SURGERY
(2020)
Article
Biochemistry & Molecular Biology
Matias Aguilera-Rojas, Soroush Sharbati, Torsten Stein, Ralf Einspanier
Article
Biology
Ying Wang, Thomas S. Chaffee, Rebecca S. LaRue, Danielle N. Huggins, Patrice M. Witschen, Ayman M. Ibrahim, Andrew C. Nelson, Heather L. Machado, Kathryn L. Schwertfeger
Article
Biochemistry & Molecular Biology
Sarah Atef Fahim, Mahmoud Salah Abdullah, Nancy A. Espinoza-Sanchez, Hebatallah Hassan, Ayman M. Ibrahim, Sarah Hamdy Ahmed, George Shakir, Mohamed A. Badawy, Nadia Zakhary, Burkhard Greve, Mohamed El-Shinawi, Martin Gotte, Sherif Abdelaziz Ibrahim
Article
Oncology
Ayman M. Ibrahim, Zane Gray, Angelica M. Gomes, Leann Myers, Fariba Behbod, Heather L. Machado
NPJ PRECISION ONCOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Ayman M. Ibrahim, Mohamed Roshdy, Sara Elshorbagy, Mohammed Hosny, Sarah Halawa, Dina Yehia, Hasnaa A. Elfawy, Ahmed Eldessouki, Faisal Mohamed, Amany Ellithy, Mohamed Abdelfattah, Amr Elsawy, Mohamed Elkhatib, Mona Allouba, Ahmed Elguindy, Yasmine Aguib, Magdi Yacoub
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Oncology
Ayman M. Ibrahim, Matthew A. Moss, Zane Gray, Michelle D. Rojo, Caitlin M. Burke, Kathryn L. Schwertfeger, Camila O. dos Santos, Heather L. Machado
FRONTIERS IN ONCOLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Hamood N. Al Kindi, Ayman M. Ibrahim, Mohamed Roshdy, Besra S. Abdelghany, Dina Yehia, Ahmed Nageeb Masoud, Walid Simry, Yasmine Aguib, Magdi H. Yacoub
Summary: This study investigated the clinical features and genetic analysis of patients with tuberous sclerosis complex presenting with large rhabdomyoma tumors, as well as the potential role of autophagy and apoptosis in tumor pathogenesis. Results showed increased expression of autophagic and apoptotic markers in tumor cells compared to normal tissue, with patients carrying pathogenic mutations in the TSC-2 gene requiring urgent intervention. Future studies may lead to personalized treatment for cardiac rhabdomyoma.
CARDIOLOGY IN THE YOUNG
(2021)
Letter
Cardiac & Cardiovascular Systems
Yasmine Aguib, Mona Allouba, Roddy Walsh, Ayman M. Ibrahim, Sarah Halawa, Alaa Afify, Mohammed Hosny, Pantazis I. Theotokis, Aya Galal, Sara Elshorbagy, Mohamed Roshdy, Heba S. Kassem, Amany Ellithy, Rachel Buchan, Nicola Whiffin, Shehab Anwer, Stuart A. Cook, Ahmed Moustafa, Ahmed ElGuindy, James S. Ware, Paul J. R. Barton, Magdi Yacoub
Article
Cardiac & Cardiovascular Systems
Sarah Halawa, Najma Latif, Yuan-Tsan Tseng, Ayman M. Ibrahim, Adrian H. Chester, Ahmed Moustafa, Yasmine Aguib, Magdi H. Yacoub
Summary: Cardiac valves - highly complex structures that interact with cells, extracellular matrix, and hemodynamic environment - are tightly regulated by various mechanisms including epigenetic factors. This study analyzed the differential methylation profiles of non-diseased human aortic and mitral valve tissue, revealing candidate genes and signaling pathways associated with valve physiology and pathology. These findings contribute to our understanding of valve biology, tissue engineering approaches, and drug target discovery.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Veterinary Sciences
Matias Aguilera-Rojas, Soroush Sharbati, Torsten Stein, Mario Candela Andrade, Barbara Kohn, Ralf Einspanier
Summary: This study investigated the effects of haemolysis on circulating miRNAs in dogs, and found that some miRNAs were significantly sensitive to haemolysis. The study also revealed differences in canine miRNA abundance compared to human miRNA. These findings have important implications for the discovery of circulating biomarkers in dogs, and emphasize the need to carefully consider the impact of haemolysis in serum or plasma-based tests.
BMC VETERINARY RESEARCH
(2022)
Article
Biology
Faizzan S. Ahmad, Yongcheng Jin, Alexander Grassam-Rowe, Yafei Zhou, Meng Yuan, Xuehui Fan, Rui Zhou, Razik Mu-u-min, Christopher O'Shea, Ayman M. Ibrahim, Wajiha Hyder, Yasmine Aguib, Magdi Yacoub, Davor Pavlovic, Yanmin Zhang, Xiaoqiu Tan, Ming Lei, Derek A. Terrar
Summary: We demonstrate a novel approach to generate atrial-like cardiomyocytes (CMs) from human-induced pluripotent stem cells (hiPSCs) through the treatment of Gremlin 2 and retinoic acid. The generated CMs exhibited similar morphology and characteristics to adult atrial myocytes (AMs), including expression of CM proteins and striated appearance. Single-cell RNA sequencing also revealed high expression of atrial-specific transcripts in the generated CMs. This method provides a valuable tool for studying atrial cell signaling pathways and atrial fibrillation (AF).
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
A. M. Ibrahim, M. Roshdy, N. Latif, P. Sarathchandra, M. Hosny, S. Haikal, A. Desouky, A. Elsawy, W. Elmozy, A. Elaithy, H. Khedr, A. Afifi, Y. Aguib, M. Yacoub
EUROPEAN HEART JOURNAL
(2022)
Article
Oncology
Ayman M. Ibrahim, Alan Bilsland, Steffen Rickelt, Joanna S. Morris, Torsten Stein
Summary: The study identified significant differences in extracellular matrix distribution in the mouse mammary gland during early pregnancy and found that pregnancy-associated fibroblasts enhanced branching initiation compared to control fibroblasts. A signature of differentially expressed matrisome RNAs was a strong prognostic indicator of distant metastasis-free survival in breast cancer patients, with particular relevance to estrogen receptor-negative breast cancers.
BREAST CANCER RESEARCH
(2021)