Article
Biochemistry & Molecular Biology
Han Wu, Zhao Wang, Song Liu, Huan Meng, Shengyuan Liu, Su Fu
Summary: Cobalt ions at sub-toxic levels impair chondrocyte mechanosensation and affect gene expression, with a mechanism involving HDAC6-dependent primary cilia disassembly and tubulin deacetylation. Pharmaceutical intervention with HDAC6 inhibitor can restore primary cilia length and mechanotransduction, while chemical depletion of primary cilia prevents mechanosignalling.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biophysics
Arianna Soave, Loraine L. Y. Chiu, Aisha Momin, Stephen D. Waldman
Summary: Mechanical stimulation in cartilage tissue engineering is widely used to enhance tissue formation and improve mechanical properties. This study investigates the use of lithium chloride treatment to restore primary cilia expression in chondrocytes and enhance mechanosensitivity, suggesting a potential new cell source for cartilage tissue engineering.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2022)
Article
Cell Biology
Irene Tirado-Cabrera, Eduardo Martin-Guerrero, Sara Heredero-Jimenez, Juan A. Ardura, Arancha R. Gortazar
Summary: Mechanical stimulation and PTH1R activation regulate osteocyte function through primary cilium, affecting communication with osteoclasts.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Neurosciences
Carlie L. Cullen, Megan O'Rourke, Shannon J. Beasley, Loic Auderset, Yilan Zhen, Renee E. Pepper, Robert Gasperini, Kaylene M. Young
Summary: Primary cilia play a crucial role in the myelination process of oligodendrocyte progenitor cells, with their assembly regulated by the gene Kif3a. Deletion of Kif3a leads to decreased OPC proliferation and oligodendrogenesis, affecting fine motor coordination in specific brain regions.
Article
Endocrinology & Metabolism
Milos Spasic, Michael P. Duffy, Christopher R. Jacobs
Summary: This study demonstrates the effectiveness of pharmacologically targeting the primary cilium to enhance bone formation. By potentiating osteocyte mechanosensing, the paracrine signaling to osteoblasts is promoted. Additionally, impairing primary cilia formation weakens this intercellular signaling cascade. In an in vivo model, fenoldopam treatment sensitizes bones to mechanical stimulation and has minimal adverse effects.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Bibiane Steinecker-Frohnwieser, Birgit Lohberger, Stefan Toegel, Reinhard Windhager, Veronika Glanz, Cornelia Kratschmann, Andreas Leithner, Lukas Weigl
Summary: Osteoarthritis is a common degenerative joint disease that causes pain and functional limitations. Physical activity is an effective intervention in alleviating pain and promoting joint function. Dysfunction of mechanosensitive ion channels in chondrocytes plays a crucial role in the progression of OA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, Research & Experimental
Yang Zhang, Godfred K. Tawiah, Xiaoan Wu, Yanjun Zhang, Xiaohu Wang, Xiaochun Wei, Xiaohong Qiao, Quanyou Zhang
Summary: Osteoarthritis (OA) is a common joint disorder associated with articular cartilage degradation and abnormal mechanical microenvironment. Primary cilia, as mechanosensory and chemosensory organelles, play important roles in chondrocytes signaling transduction and matrix homeostasis. The ability of primary cilium to regulate cellular signaling depends on the matrix microenvironment.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Clare L. Thompson, Megan McFie, J. Paul Chapple, Philip Beales, Martin M. Knight
Summary: This study demonstrates the essential role of polycystins in chondrocyte mechanotransduction and highlights the importance of PC2 cilia trafficking as a key component of cilia-mediated mechanotransduction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Yu Qin, Xue-Bo Hu, Wen-Ting Fan, Jing Yan, Shi-Bo Cheng, Yan-Ling Liu, Wei-Hua Huang
Summary: A stretchable and multifunctional platform integrating 3D cell culture, mechanical loading, and electrochemical sensing has been developed for the first time, showing promising potential in exploring mechanotransduction in 3D cellular systems and engineered tissues. The platform utilizes biomimetic peptide linked gold nanotubes on porous and elastic polydimethylsiloxane to mimic articular cartilage and investigate mechanotransduction pathways in chondrocytes. The results reveal previously unclear mechanisms, such as the rapid activation of nitric oxide signaling by mechanical loading in chondrocytes.
Article
Biochemistry & Molecular Biology
Jinbiao Chen, Ngan Ching A. Cheng, Jade Boland, Ken G. Liu, James Kench, D. Neil Watkins, Sofia J. Ferreira-Gonzalez, Stuart W. Forbes, Geoffrey McCaughan
Summary: Loss of primary cilia in biliary epithelial cells resulted in the formation of cystic lesions during TAA treatment, without affecting the progression to cirrhosis. The cystic structures showed increased cellular proliferation and decreased hepatocyte lobular proliferation. Loss of primary cilia initially decreased cell passage survival in biliary organoids, but this effect was diminished in later passages.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Multidisciplinary Sciences
Alireza Savadipour, Robert J. Nims, Neda Rashidi, Jaquelin M. Garcia- Castorena, Ruhang Tang, Gabrielle K. Marushack, Sara J. Oswald, Wolfgang B. Liedtke, Farshid Guilak
Summary: Through AFM and FEM studies, we found that PIEZO1 and PIEZO2 mediate calcium (Ca2+) signaling in chondrocytes at moderately high levels of cellular deformation, while at the highest strains, PIEZO1 functions independently of PIEZO2. Biophysical factors that increase apparent chondrocyte membrane tension also enhance PIEZO1-driven Ca2+ signaling. We identified a threshold of membrane stretch ratio that leads to PIEZO1-induced chondrocyte mechanical injury, suggesting potential targets for osteoarthritis prevention or treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Pathology
David Grevenstein, Johannes Oppermann, Lina Winter, Friedrich Barsch, Tanja Niedermair, Andreas Mamilos, Peer Eysel, Christoph Brochhausen
Summary: The anterior cruciate ligament (ACL) plays a significant role in knee stability and proprioception, and the presence of primary cilia (PC) in human ACL tissue is explored in this study. The study found a lower rate of PC positive cells in ACL tissue compared to other tissues and ligaments of other species.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Review
Oncology
Neha Quadri, Priyanka Upadhyai
Summary: Primary cilia play a fundamental role in vertebrate cells, modulating organismal development, morphogenesis, and repair. This article focuses on their involvement in skeletal development, examining evidence of their role in signaling pathways and their relevance to skeletal disorders. The authors discuss the molecular factors and mechanisms governing primary ciliogenesis and ciliary function in skeletal development and disease.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Weijun Li, Zhenhong Zhu, Kai He, Xiaoyu Ma, Robert J. Pignoloa, Gary C. Sieck, Jinghua Hu, Haitao Wang
Summary: Skeletal muscle atrophy is associated with aging and other factors. Mature muscle cells from skeletal muscle satellite cells play a crucial role in repairing muscle tissue. Exercise therapy promotes muscle hypertrophy and strength. This study demonstrates that the primary cilium in skeletal muscle cells acts as a mechanical sensor and promotes exercise-induced muscle hypertrophy through the Hedgehog signaling pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Biomedical
Chenchen Zhou, Yueyi Yang, Mengmeng Duan, Cheng Chen, Caixia Pi, Demao Zhang, Xiaoheng Liu, Jing Xie
Summary: This study designs a novel patterned equidistant micropillar substrate based on PDMS to mimic the collagen fiber-like network of cartilage matrix, and finds that chondrocytes seeded onto the soft micropillar substrate maintain their phenotype more effectively. Chondrocytes sense and respond to micropillar substrates with different stiffnesses by altering the ECM-cytoskeleton-focal adhesion axis. This study provides a model to understand the cell behavior changes during the transition from a normal state to a state of osteoarthritis.
ADVANCED HEALTHCARE MATERIALS
(2023)