Review
Immunology
Peilong Li, Nan Wang, Yi Zhang, Chuanxin Wang, Lutao Du
Summary: HLA-G, a non-classic MHC class I molecule, is widely expressed in tumor environment where it plays a key role in immune escape, potentially important for cancer diagnosis and prognosis. However, due to its multiple subtypes, evaluating HLA-G expression in tumors is challenging.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Francois Anna, Elodie Bole-Richard, Joel LeMaoult, Marie Escande, Martin Lecomte, Jean-Made Certoux, Philippe Souque, Francine Garnache, Olivier Adotevi, Pierre Langlade-Demoyen, Maria Loustau, Julien Caumartin
Summary: CAR-T cell immunotherapy is a breakthrough in treating hematological malignancies, but the lack of tumor-specific antigens and immunosuppressive tumor microenvironments remain major hurdles. A new study shows that HLA-G, acting as both an immune checkpoint and a tumor-specific antigen, could be a promising target for CAR-T cell therapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Immunology
Arthur A. A. Cross-Najafi, Kristine Farag, Abdulkadir Isidan, Wei Li, Wenjun Zhang, Zhansong Lin, Julia R. R. Walsh, Kevin Lopez, Yujin Park, Nancy G. G. Higgins, David K. C. Cooper, Burcin Ekser, Ping Li
Summary: Natural killer (NK) cells play a key role in immune rejection in solid organ transplantation. In this study, the expression of inhibitory ligands (HLA-E and HLA-G) on genetically modified porcine endothelial cells was found to significantly reduce NK cell activation and improve xenograft survival. Co-expression of HLA-E and HLA-G on pig cells provided superior inhibition compared to individual expression, leading to decreased human NK cell activation. This in vitro study demonstrates the potential of genetic engineering of pigs to prevent human NK cell-mediated rejection.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Siqi Liu, Nicolaas A. Bos, Erik A. M. Verschuuren, Debbie van Baarle, Johanna Westra
Summary: The article discusses the importance and significance of HLA-G in transplantation as a crucial molecule in combating rejection reactions. During pregnancy, it also helps maintain immune tolerance between mother and fetus.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Monika Zaborek-Lyczba, Jakub Lyczba, Paulina Mertowska, Sebastian Mertowski, Anna Hymos, Martyna Podgajna, Paulina Niedzwiedzka-Rystwej, Ewelina Grywalska
Summary: HLA-G, a non-classical MHC class I molecule, has been studied for its relevance in pathological conditions like autoimmune diseases. Current research aims to utilize HLA-G in clinical practice for diagnostics and therapeutic purposes, as it is known for its immunomodulatory role in reducing inflammation and immune responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Simon Jasinski-Bergner, Dominik Schmiedel, Ofer Mandelboim, Barbara Seliger
Summary: This review provides a summary of the expression, regulation, function, and impact of HLA-G in the context of different viral infections, including virus-associated cancers. It emphasizes the potential use of HLA-G as a novel diagnostic and prognostic biomarker for viral infections, as well as a therapeutic target.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Baimei Zhuang, Jin Shang, Yuanqing Yao
Summary: HLA-G plays a crucial role in maternal-fetal immune tolerance, maintaining immune homeostasis and considered a key factor for successful pregnancies. Understanding the characteristics of HLA-G is important for treating pathological pregnancies and other immune-related diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Guoxu Zheng, Lintao Jia, An-Gang Yang
Summary: HLA-G/KIR2DL4 plays an important role in breast cancer, serving as a key factor in drug resistance and immune evasion. Research on HLA-G is beneficial for identifying new biomarkers for breast cancer, improving the accuracy of diagnosis and prognostic assessment. Additionally, unraveling the mechanisms underlying HLA-G/KIR2DL4 regulation of the immune microenvironment in breast cancer provides a theoretical basis for combined HLA-G and immune checkpoint targeting.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Hui-Hui Xu, Jun Gan, Dan-Ping Xu, Lu Li, Wei-Hua Yan
Summary: The study comprehensively analyzed the role of HLA-G in various types of cancer, revealing associations with immune checkpoints, pathological stages, prognosis, and tumor-infiltrating immune cells. The differential expression of HLA-G across different tumors may be explained by multiple mutations and methylation status. Functional enrichment analysis showed that HLA-G is primarily related to T cell-mediated immunity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Zhansong Lin, Arman A. Bashirova, Mathias Viard, Lee Garner, Max Quastel, Maya Beiersdorfer, Wojciech K. Kasprzak, Marjan Akdag, Yuko Yuki, Pedro Ojeda, Sudipto Das, Thorkell Andresson, Vivek Naranbhai, Amir Horowitz, Andrew J. McMichael, Angelique Hoelzemer, Geraldine M. Gillespie, Wilfredo F. Garcia-Beltran, Mary Carrington
Summary: Human leukocyte antigen (HLA)-E binds epitopes derived from HLA-A, HLA-B, HLA-C and HLA-G signal peptides (SPs) and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors. Carrington and colleagues provide comprehensive analysis of classical HLA class I SP variants and show that these can determine CD94/NKG2-HLA-E engagement. The systematic, quantitative approach described herein will facilitate development of prediction algorithms for accurately measuring the impact of CD94/NKG2-HLA-E interactions in disease resistance/susceptibility.
Article
Biochemistry & Molecular Biology
Pascal Pedini, Lucas Hubert, Federico Carlini, Jean Baptiste Baudey, Audrey Tous, Francois Jordier, Agnes Basire, Claude Bagnis, Martine Reynaud-Gaubert, Benjamin Coiffard, Jacques Chiaroni, Monique Silvy, Christophe Picard
Summary: Lung transplantation is often complicated by acute and/or chronic rejection leading to graft-function loss. In addition to the HLA donor-specific antibodies (HLA-DSA), a few autoantibodies are correlated with the occurrence of these complications. Recently, antibodies directed against non-classical HLA molecules, HLA-G, -E, and -F have been detected in autoimmune diseases, like systemic lupus erythematosus. Non-classical HLA molecules are crucial in the immunological acceptance of the lung graft, and some of their isoforms, like HLA-G*01:04 and -G*01:06, are associated with a negative clinical outcome. The aim of this study is to determine the frequency of detection of HLA-G antibodies in lung transplant recipients (LTRs) and their impact on the occurrence of clinical complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Fabio Morandi, Irma Airoldi
Summary: HLA-G is an HLA-class Ib molecule involved in tolerance establishment during pregnancy. The expression of HLA-G is restricted in adults but can be observed in tumors, where it contributes to immune escape and tumor progression. It interacts with other immune checkpoint molecules like PD-1, CTLA-4, TIM-3, IDO, and TIGIT. Clinical trials using monoclonal antibodies against HLA-G and other immune checkpoints have shown promising results in cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Jia Mao, Ying Feng, Xiaofeng Zhu, Fang Ma
Summary: Human leukocyte antigen-G (HLA-G) is a non-classical major histocompatibility complex molecule that plays a crucial role in immune regulation at the maternal-fetal interface. This review highlights the different molecular mechanisms by which HLA-G modulates the immune function of NK cells, including the suppression of cytotoxicity and the stimulation of growth factor secretion. The interaction between HLA-G and NK cells also promotes senescence signaling and inhibits the function of other immune cells, contributing to spiral artery remodeling and maintaining immune balance during pregnancy.
Article
Biochemistry & Molecular Biology
Ruonan Xu, Ji-Yuan Zhang, Bo Tu, Zhe Xu, Hui-Huang Huang, Lei Huang, Yan-Mei Jiao, Tao Yang, Chao Zhang, En-Qiang Qin, Tian-Jun Jiang, Yun-Bo Xie, Yuan-Yuan Li, Lei Jin, Chun-Bao Zhou, Ming Shi, Mei Guo, Hui-Sheng Ai, Linqi Zhang, Fu-Sheng Wang
Summary: This study reports the development of an allogeneic adaptive immune therapy for severely immunosuppressed AIDS patients, showing safety and efficacy in combination with antiretroviral therapy. The therapy involved transfusing peripheral blood mononuclear cells from qualified donors into patients, leading to improvements in immune function and clinical symptoms. Further clinical trials with control groups are needed to confirm the efficacy of this medication.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Article
Surgery
Thomas Jouve, Caroline Laheurte, Johan Noble, Jules Weinhard, Melanie Daligault, Adeline Renaudin, Hamza Naciri Bennani, Dominique Masson, Eleonore Gravelin, Mathilde Bugnazet, Beatrice Bardy, Paolo Malvezzi, Philippe Saas, Lionel Rostaing
Summary: In highly sensitized kidney transplant candidates, tocilizumab as a monotherapy limited B cell maturation while having almost no effect on anti-HLA alloantibodies.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Oncology
F. Ayala de la Pena, B. Ortiz-Munoz, T. Quintanar-Verduguez, J. D. Santotoribio, S. de la Cruz, J. Trape-Pujol, E. Galve-Calvo, J. M. Auge-Fradera, J. Garcia-Gomez, A. Gonzalez-Hernandez
Summary: This article summarizes the use and interpretation of circulating tumor markers in breast cancer, focusing on their relationship with molecular subtypes and prognostic value. It also proposes a series of recommendations to improve the clinical utility of TMs.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Monica Macias, Angel Garcia-Cortes, Marcos Torres, Javier Ancizu-Marckert, Juan Ignacio Pascual, Fernando Diez-Caballero, Jose Enrique Robles, David Rosell, Bernardino Minana, Beatriz Mateos, Daniel Ajona, Rodrigo Sanchez-Bayona, Oihane Bedialauneta, Susana Chocarro, Ana Navarro, Maria P. Andueza, Alfonso Gurpide, Jose Luis Perez-Gracia, Estibaliz Alegre, Alvaro Gonzalez
Summary: This study demonstrates that certain cytokine levels in preoperative exosomes of prostate cancer patients correlate with factors such as PSA and Gleason score, showing the important role of exosomes in MDSC recruitment.
Article
Biotechnology & Applied Microbiology
Carmen Campos-Silva, Yaiza Caceres-Martell, Estela Sanchez-Herrero, Amaia Sandua, Alexandra Beneitez-Martinez, Alvaro Gonzalez, Mariano Provencio, Atocha Romero, Ricardo Jara-Acevedo, Maria Yanez-Mo, Mar Vales-Gomez
Summary: This study describes a method for identifying tumor markers on EVs using only a small amount of patient's plasma. By studying the physico-chemical properties of EVs in solution, it was found that cationic polymers can increase the sedimentation coefficient of EVs, thereby improving their capture efficiency in immunocapture assays. By optimizing the protocol, EVs can be effectively captured directly in plasma without the need for ultracentrifugation or other EV enrichment.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Diana Tronik-Le Roux, Marina Daouya, Alix Jacquier, Chantal Schenowitz, Francois Desgrandchamps, Nathalie Rouas-Freiss, Edgardo D. Carosella
Summary: The heterogeneity of cancer cells poses challenges in therapeutic approaches, and the newly discovered isoform HLA-G increment alpha 1 has immune-stimulatory activity in ccRCC and trophoblasts. Unlike other HLA-G isoforms, HLA-G increment alpha 1 does not associate with beta 2-microglobulin and may not present antigens to T cells. It exerts individual dependent immune-stimulatory effects on peripheral blood NK cells and might have potential antitumor effects.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Medical Laboratory Technology
Estibaliz Alegre, Nerea Varo, Pilar Fernandez-Calle, Sofia Calleja, Alvaro Gonzalez
Summary: This study evaluated the long-term storage stability of different analytes at ultra-low temperatures. The results showed significant variations in some analytes, highlighting the importance of considering this uncertainty. To ensure accurate analysis, the reference change value formula should include preanalytical variability.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Catia Monteiro, Lauritz Miarka, Maria Perea-Garcia, Neibla Priego, Pedro Garcia-Gomez, Laura Alvaro-Espinosa, Ana de Pablos-Aragoneses, Natalia Yebra, Diana Retana, Patricia Baena, Coral Fustero-Torre, Osvaldo Grana-Castro, Kevin Troule, Eduardo Caleiras, Patricia Tezanos, Pablo Muela, Elisa Cintado, Jose Luis Trejo, Juan Manuel Sepulveda, Pedro Gonzalez-Leon, Luis Jimenez-Roldan, Luis Miguel Moreno, Olga Esteban, Angel Perez-Nunez, Aurelio Hernandez-Lain, Jose Mazarico Gallego, Irene Ferrer, Rocio Suarez, Eva M. Garrido-Martin, Luis Paz-Ares, Celine Dalmasso, Elizabeth Cohen-Jonathan Moyal, Aurore Siegfried, Aisling Hegarty, Stephen Keelan, Damir Vareslija, Leonie S. Young, Malte Mohme, Yvonne Goy, Harriet Wikman, Jose Fernandez-Alen, Guillermo Blasco, Lucia Alcazar, Clara Cabanuz, Sergei Grivennikov, Andrada Ianus, Noam Shemesh, Claudia C. Faria, Rebecca Lee, Paul Lorigan, Emilie Le Rhun, Michael Weller, Riccardo Soffietti, Luca Bertero, Umberto Ricardi, Joaquim Bosch-Barrera, Elia Sais, Eduard Teixidor, Alejandro Hernandez-Martinez, Alfonso Calvo, Javier Aristu, Santiago M. Martin, Alvaro Gonzalez, Omer Adler, Neta Erez, Manuel Valiente
Summary: This study identifies a molecular mechanism underlying resistance to whole-brain radiotherapy and suggests potential targets and biomarkers for personalized radiotherapy. The findings have important implications for improving the efficacy of treatment for patients with brain metastasis.
Article
Multidisciplinary Sciences
Estibaliz Alegre, Amaia Sandua, Sofia Calleja, Sara Deza, Alvaro Gonzalez
Summary: Breath tests for evaluating carbohydrate malabsorption require low baseline H-2 and CH4 levels. Light walking can reduce these levels and improve test accuracy.
SCIENTIFIC REPORTS
(2022)
Editorial Material
Medical Laboratory Technology
Estibaliz Alegre, Henar Casal, Juan C. Galofre, Alvaro Gonzalez
CLINICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Leire Fernandez-Ciriza, Alvaro Gonzalez, Jose Luis del Pozo, Alejandro Fernandez-Montero, Francisco Carmona-Torre, Silvia Carlos, Maria del Mar Sarasa, Gabriel Reina
Summary: Scarce data on cellular immunity and longevity for different COVID-19 vaccination schedules have been reported. A prospective study was conducted on healthcare workers receiving various COVID-19 vaccines to compare the immune response over a span of 9 months. The study found that individuals with hybrid immunity from receiving one dose of any vaccine had higher levels of SARS-CoV-2 spike antibodies compared to those uninfected individuals who received two doses. Naturally infected individuals did not show a substantial increase in antibody titers after the second dose, and their antibody levels remained higher throughout the 9-month follow-up. Cellular immunity was preserved in a high percentage of individuals who received mRNA-based vaccines.
SCIENTIFIC REPORTS
(2022)
Article
Medical Laboratory Technology
Amaia Sandua, Miguel F. Sanmamed, Maria Rodriguez, Javier Ancizu-Marckert, Alfonso Gurpide, Jose L. Perez-Gracia, Estibaliz Alegre, Alvaro Gonzalez
Summary: This study characterized PSA in extracellular microvesicles (EVs) and compared its reactivity to commercial methods. Results showed a positive correlation between PSA in EVs and serum PSA, with lower levels in prostate cancer patients. Additionally, commercial methods detected higher concentrations of PSA in EVs compared to traditional methods.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Victoria Michaels, Smahane Chalabi, Agnes Legrand, Julie Renard, Emmanuel Tejerina, Marina Daouya, Sylvie Fabrega, Jerome Megret, Robert Olaso, Anne Boland, Jean-Francois Deleuze, Christophe Battail, Diana Tronik-Le Roux, Sophie Ezine
Summary: T cells have the potential to maintain immunological memory and self-tolerance. Delayed T cell reconstitution is a common issue in hematopoietic stem cell transplantation. To overcome this difficulty, a new approach using DNA barcoding strategy was developed to identify populations with efficient lymphoid reconstitution properties. Results highlight the role of LMPP progenitors for lymphoid generation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Julia Maroto-Garcia, Sara Deza, Patricia Fuentes-Bullejos, Patricia Fernandez-Tomas, Debora Martinez-Espartosa, Maria Marcos-Jubilar, Nerea Varo, Alvaro Gonzalez
Summary: The study compares the preanalytical and analytical performance of remote self-collected capillary blood samples and venous blood samples. It also examines the stability of common measurands in capillary blood. The results show higher hemolysis index in capillary samples, but no bias was found in the biochemistry and hematologic parameters between the two types of samples, except for mean corpuscular volume (MCV). The study suggests cautiousness when analyzing capillary samples beyond 24 hours of collection.
Article
Medical Laboratory Technology
Imma Caballe, Antonio Buno, Francisco A. Bernabeu, Francesca Canalias, Antonio Moreno, Merce Ibarz, Jose Puzo, Concepcion Gonzalez, Alvaro Gonzalez
Summary: Laboratory medicine is a crucial discipline in healthcare that plays a significant role in the diagnosis, management, and monitoring of patients. However, it faces challenges such as the advancement of technology and increased demand. This study provides valuable insights into the state of clinical laboratories and professionals in Spain.
ADVANCES IN LABORATORY MEDICINE-AVANCES EN MEDICINA DE LABORATORIO
(2023)