Article
Toxicology
S. G. Rudyak, L. A. Usakin, E. A. Tverye, E. D. Robertson, A. A. Panteleyev
Summary: Aryl hydrocarbon receptor (AhR) expression and stability are influenced by AhR ligands. Treatment with TCDD, FICZ, and I3C caused a decrease in AhR expression. AhR degradation is regulated by proteasomal degradation and the presence of AhR antagonists, and requires the involvement of AhR dimerization partner ARNT. In addition, HDAC inhibition enhances AhR expression.
TOXICOLOGY LETTERS
(2023)
Article
Cell Biology
Tu Nguyen, Mei Zheng, Maura Knapp, Nikola Sladojevic, Qin Zhang, Lizhuo Ai, Devin Harrison, Anna Chen, Albert Sitikov, Le Shen, Frank J. Gonzalez, Qiong Zhao, Yun Fang, James J. K. Liao, Rongxue Wu
Summary: The decline in endothelial ARNT expression contributes to the suppressed angiogenic phenotype in diabetic mice. The cytoprotective effect of ARNT in endothelial cells is partially mediated by declines in ROS production.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Yujing Bi, Qiuli Yang, Zhengchao Li, Yuexin Wang, Yufei Wang, Anna Jia, Zhiyuan Pan, Ruifu Yang, Guangwei Liu
Summary: The lack of aryl hydrocarbon receptor nuclear translocator (ARNT) promotes neutrophil recruitment, neutrophil extracellular trap (NET) development, inflammatory cytokine secretion, and suppressive activities, thereby promoting colorectal cancer growth. The gut microbiota plays a crucial role in the functional alterations of Arnt(-/-) neutrophils. These findings have important implications for the combination of gut microbiota and immunotherapy approaches in colorectal cancer.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Bohao Yin, Zhiyuan Fan, Yingzhe Jin, Hongchi Chen, Baokun Zhang, Jingwen Liu, Wei Zhang
Summary: This study investigated the cellular properties of EGF/EGFR in osteoblasts, revealing EGF internalization into the cytoplasm under EGFR mediation and the critical role of caveolin in EGFR endocytosis. Furthermore, the analysis showed EGFR entering into Rab5, Rab4, and Rab9-positive endosomes, and even moving into the nuclei of MC3T3-E1 cells. The findings suggest that nuclear-localized EGFR has important biological functions, laying a foundation for further research on EGF/EGFR's biological functions on osteoblasts.
Article
Multidisciplinary Sciences
Gunsmaa Nyamsuren, Gregor Rapp, Hassan Dihazi, Elisabeth M. Zeisberg, Desiree Tampe, Bjoern Tampe, Michael Zeisberg
Summary: Phosphorylation of the Ser77 residue is critical for ARNT-ARNT homodimer formation and stabilization, and inhibition of PP2A phosphatase activity can enhance the formation of ARNT-ARNT homodimers. Combinations of FK506 or GPI1046 with LB100 elicit additive anti-fibrotic activities in murine models of kidney and liver fibrosis.
SCIENTIFIC REPORTS
(2021)
Article
Dermatology
Carrie Hayes Sutter, Shafquat Azim, Anyou Wang, Jyoti Bhuju, Amelia S. Simpson, Aayushi Uberoi, Elizabeth A. Grice, Thomas R. Sutter
Summary: Activation of the aryl hydrocarbon receptor (AHR) promotes keratinocyte differentiation and the formation of the epidermal permeability barrier. Lipids, including ceramides, play a critical role in the epidermal permeability barrier. Researchers found that AHR ligand increased the RNA levels of ceramide metabolism and transport genes in human epidermal keratinocytes, as well as the levels of skin ceramides. They identified UGCG as a direct target of AHR and demonstrated that AHR regulates the expression of UGCG, an enzyme involved in ceramide trafficking, keratinocyte differentiation, and epidermal permeability barrier formation.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Oncology
Michelle M. Steidemann, Jian Liu, Kalin Bayes, Lizbeth P. Castro, Shelagh Ferguson-Miller, John J. Lapres
Summary: Cellular homeostasis requires the use of multiple environmental sensors to respond to different compounds. The aryl hydrocarbon receptor (AHR) and the translocator protein (TSPO) are two proteins involved in cellular homeostasis, and their interaction affects mitochondrial-related genes. Knockouts for AHR and TSPO were created in a mouse lung epithelial cell line, and the cells' response to ligands for AHR and TSPO was analyzed through RNA-seq. The results suggest that AHR and TSPO participate in similar pathways that contribute to mitochondrial homeostasis.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Eva Maria Rico-Leo, Luis Francisco Lorenzo-Martin, Angel Carlos Roman, Xose Ramon Bustelo, Jaime Maria Merino, Pedro Maria Fernandez-Salguero
Summary: The aryl hydrocarbon receptor (AhR) plays a crucial role in skin regeneration and homeostasis by maintaining the identity of epidermal stem cells, making it a potential target for treating cutaneous pathologies.
Article
Chemistry, Medicinal
Nur Shazwani Mohd Pilus, Azira Muhamad, Muhammad Ashraf Shahidan, Nurul Yuziana Mohd Yusof
Summary: Sea cucumber is commonly used in traditional Asian medicine for wound healing due to its strong regenerative ability. A short peptide called Sh-EGFl-1 has been found to promote the growth of human melanoma cells. It interacts favorably with the human epidermal growth factor receptor and activates multiple epidermal growth factor signaling pathways, leading to cell proliferation, differentiation, survival, and actin cytoskeleton reorganization. This marine-derived peptide has potential as a cell-growth-promoting agent for biomedical and bioprocessing applications.
Article
Gastroenterology & Hepatology
Puneet Kumar, Manoj Yadav, Khushi Verma, Ruhi Dixit, Juhi Singh, Satyendra K. Tiwary, Gopeshwar Narayan, V. K. Dixit
Summary: The study reveals that AHRR expression is significantly down-regulated in gallbladder cancer tissue samples compared to controls, and this decreased expression is associated with tumor development and progression, suggesting a potential role for AHRR as a tumor suppressor gene in gallbladder cancer.
SAUDI JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Environmental Sciences
Min-Kyung Cho, Jae-Gon Park, Hisato Iwata, Eun-Young Kim
Summary: Studies have revealed that exposure to TCDD resulted in severe weight loss and thymus atrophy in chicken embryos, indicating that TCDD affects the immune system of chicken embryos by suppressing T cell-mediated immune responses. Thymic transcriptome analysis suggested that TCDD-induced thymus atrophy may be associated with alterations in multiple signaling pathways, leading to significant changes in the expression levels of immune-related genes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Yan-Ni Wang, Hua Miao, Xiao-Yong Yu, Yan Guo, Wei Su, Fei Liu, Gang Cao, Ying-Yong Zhao
Summary: Patients with membranous nephropathy (MN) are diagnosed by the presence of phospholipase A2 receptor (PLA2R) before they progress to renal failure. The aryl hydrocarbon receptor (AHR), NF-kB, and Nrf2 pathways are involved in the pathogenesis and progression of chronic kidney disease. This study investigated the effect of AHR signalling on the NF-kB and Nrf2 pathways in MN patients, rats, and podocytes.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Chun-Hua Lin, Chia-Cheng Chou, Yi-Hsuan Lee, Chia-Chi Hung
Summary: Curcumin exerts anti-inflammatory effects by binding to AhR and inhibiting inflammatory astrocyte response. Despite the enhanced expression of AhR and its target genes induced by curcumin, the precise mechanisms of AhR involvement in curcumin's anti-inflammatory effects require further investigation.
Review
Biochemistry & Molecular Biology
Alevtina Y. Grishanova, Maria L. Perepechaeva
Summary: This review examines the various mechanisms by which AhR participates in the oxidative stress response, including antioxidant and prooxidant enzymes and cytochrome P450. AhR, as a participant in the redox balance and as a modulator of redox signals, is being increasingly studied as a target for a new class of therapeutic compounds and as an explanation for the pathogenesis of some disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lisa Goedtke, Heike Sprenger, Ute Hofmann, Felix F. Schmidt, Helen S. Hammer, Ulrich M. Zanger, Oliver Poetz, Albrecht Seidel, Albert Braeuning, Stefanie Hessel-Pras
Summary: PAHs are environmental pollutants generated by incomplete combustion of organic matter, which can induce their own metabolism by activating AHR and CAR. Most PAHs activate AHR, while non-carcinogenic PAHs tend to activate CAR, leading to the expression of respective target genes. Additionally, an increased PAH ring number is associated with AHR activation and DNA double-strand breaks, while smaller PAHs activate CAR but do not cause DNA damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Y-H Liao, K-H Chiang, J-M Shieh, C-R Huang, C-J Shen, W-C Huang, B-K Chen
Article
Oncology
Wei-Chiao Chen, Wen-Chang Wang, Hsing-Fang Lu, Yukinori Okada, Wei-Pin Chang, Yii-Her Chou, Hui-Hua Chang, Jin-Ding Huang, Der-Yuan Chen, Wei-Chiao Chang
Article
Genetics & Heredity
Yu-Jia Wang, Kai-Yun Chen, Li-Na Kuo, Wen-Chang Wang, Yu-Wen Hsu, Henry Sung-Ching Wong, Chien-Min Lin, Kuo-Hsing Liao, Yan-Feng Zhang, Yung-Hsiao Chiang, Wei-Chiao Chang
BMC MEDICAL GENETICS
(2018)
Meeting Abstract
Oncology
Chih-Jie Shen, Shih-Hung Chan, Chung-Ta Lee, Wan-Chen Huang, Jhih-Peng Tsai, Kwang-Yu Chang, Wen-Chang Chang, Ben-Kuen Chen
Article
Behavioral Sciences
Hsiang-Wen Hsieh, Shu-Chun Chen, Wan-Chen Huang, Shu Fang, En-Cheng Yang, Chu-Chun Hsu, Rong Kou
Summary: The study reveals an increase in tryptophan and tyrosine levels in the hemolymph of male Nauphoeta cinerea after social contact, with different responses in dominants and subordinates. Additionally, different outcomes were observed after male-female interactions, with varying levels of amino acids and behaviors observed. The results suggest a multi-dimensional physiological response to social contact in N. cinerea, alongside previously characterized responses in pheromone and juvenile hormone levels.
HORMONES AND BEHAVIOR
(2021)
Article
Health Care Sciences & Services
Yu-Jia Wang, Eko Mugiyanto, Yun-Ting Peng, Wan-Chen Huang, Wan-Hsuan Chou, Chi-Chiu Lee, Yu-Shiuan Wang, Lalu Muhammad Irham, Dyah Aryani Perwitasari, Ming- Hsu, Wei-Chiao Chang
Summary: The study reveals that the WDR4 gene is associated with asthenozoospermia, a common clinical manifestation of male infertility, especially the SNP rs465663. Furthermore, low expression of WDR4 contributes to ROS-induced DNA fragmentation, suggesting a critical role of WDR4 gene variant and protein expression in asthenozoospermia.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Medicine, Research & Experimental
Yu-Shiuan Wang, Nai-Kuei Huang, Yu-Chiao Lin, Wei-Chiao Chang, Wan-Chen Huang
Summary: Store-operated Ca2+ channel (SOC)-regulated Ca2+ entry is crucial in inflammation and colorectal cancer progression, with nonsteroidal anti-inflammatory drugs (NSAIDs) having potential for new treatments. Testing eight NSAIDs, Aspirin and Sulindac were found to be most effective at suppressing SOC activity via different mechanisms. Time-lapse imaging showed how Aspirin disrupted STIM1-Orai1 interaction and Sulindac suppressed STIM1 translocation, with both drugs inhibiting metastasis in CRC cells without affecting cell survival. This study sheds light on the therapeutic potential of Aspirin in treating CRC and SOCE-related diseases.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Chemistry, Medicinal
Yu-Shiuan Wang, Teng-Kuang Yeh, Wei-Chiao Chang, Jing-Ping Liou, Yi-Min Liu, Wan-Chen Huang
Summary: Ca2+ entry from store-operated Ca2+ channel (SOC) is involved in regulating colorectal cancer progression. In this study, compound 2,6-Difluoro-N-(5-(4-fluorophenyl)pyridine-2-yl)benzamide (MPT0M004, 8a) demonstrated prominent inhibitory ability on SOC and lower cytotoxicity compared to 2-APB. It has the potential to be developed into a drug for treating colorectal cancer.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Oncology
Eko Mugiyanto, Wirawan Adikusuma, Lalu Muhammad Irham, Wan-Chen Huang, Wei-Chiao Chang, Chun-Nan Kuo
Summary: Through the utilization of genomic information, we have identified two promising drugs for pancreatic cancer treatment and proposed the concept of drug repurposing.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Yu-Wen Hsu, Henry Sung-Ching Wong, Wan-Chen Huang, Yi-Hung Yeh, Chwan-Deng Hsiao, Wei-Chiao Chang, Shie-Liang Hsieh
Summary: Using multiomics and phenome-wide correlations, we identified a genetic variant (rs75776403) in the CLEC18A gene that regulates human complex traits and diseases through disruptions in lipid-binding ability of the protein domain.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Proceedings Paper
Engineering, Electrical & Electronic
Han-Jung Liao, Jean An Chieh, Yu-Chen Chen, Kang-Yun Lee, Yao-Fei Chan, Shu-Chuan Ho, Wei-lun Sun, Yu-Shiuan Wang, Wan-Chen Huang, Wei-Chiao Chang, Cheng-Hsien Liu
Summary: Immunotherapy enhances T cell activity to treat cancer, with drugs disrupting the PD-1-PD-L1 interaction showing promise. A microfluidic device was developed to mimic tumor tissue environment and test multiple drug concentrations efficiently, showing cell survival and T cell infiltration.
2021 21ST INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS)
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Yu-Chen Chen, Han-Jung Liao, Jean-An Chich, Pin-Tzu Lai, Yi-Ying Liang, Kang-Yun Lee, Wei-Lun Sun, Shu-Chuan Ho, Yu-Shiuan Wang, Wan-Chen Huang, Wei-Chiao Chang, Sung-Yang Wei, Cheng-Hsien Liu
Summary: This study developed a novel microfluidic device combining GelMA and collagen biomaterials for patterning and cultivation of cancer cells in a 3D environment. Experimental results demonstrated the potential of this device for future clinical cancer research applications and exploration of interactions between cell models and drugs.
2021 21ST INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS)
(2021)
Article
Pharmacology & Pharmacy
Lalu Muhammad Irham, Henry Sung-Ching Wong, Wan-Hsuan Chou, Wirawan Adikusuma, Eko Mugiyanto, Wan-Chen Huang, Wei-Chiao Chang
PHARMACOLOGICAL RESEARCH
(2020)
Article
Biology
Lalu Muhammad Irham, Wan-Hsuan Chou, Yu-Shiuan Wang, Wirawan Adikusuma, Henry Sung-Ching Wong, Dyah Aryani Perwitasari, Wan-Chen Huang, Ben-Kuen Chen, Hwai- Yang, Wei-Chiao Chang
Article
Cell Biology
Yi-Chao Lee, Wan-Chen Huang, Jiann-Her Lin, Tzu-Jen Kao, Hui-Ching Lin, Kuen-Haur Lee, Hsin-Chuan Lin, Che-Kun James Shen, Wen-Chang Chang, Chi-Chen Huang
JOURNAL OF BIOMEDICAL SCIENCE
(2018)