Article
Plant Sciences
Kyle Symonds, Howard Teresinski, Bryan Hau, David Chiasson, Kirsten Benidickson, William Plaxton, Wayne A. Snedden
Summary: Calmodulin-like proteins (CMLs) are the largest family of calcium-binding proteins in plants. The study found that CML13 and CML14 play important roles in plant development, regulating development and morphological features through interactions with other proteins. Inhibition of CML13 or CML14 transcript levels leads to abnormal plant development, including difficulties in germination, chlorosis, and leaf senescence.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Biology
Eider Nunez, Frederick Jones, Arantza Muguruza-Montero, Janire Urrutia, Alejandra Aguado, Covadonga Malo, Ganeko Bernardo-Seisdedos, Carmen Domene, Oscar Millet, Nikita Gamper, Alvaro Villarroel, Henry M. Colecraft
Summary: Neuronal K(V)7 channels are highly sensitive to reactive oxygen species, and the S2S3 linker of the voltage sensor plays a crucial role in redox modulation of the channels. Recent structural studies have revealed interactions between this linker and the Ca2+-binding loop of calmodulin, specifically the EF3 hand, which is essential for the signaling. Disrupting Ca2+ binding to EF3 abolishes the oxidation-induced enhancement of K(V)7.4 currents. Furthermore, the loading of EF3 with Ca2+ is critical for the reorientation of the AB fork, which translates the Ca2+ signals.
Review
Cell Biology
Vanessa M. Ruscetta, Taj J. J. Seaton, Aleen Shakeel, Stanley N. S. Vasconcelos, Russell D. D. Viirre, Marc J. J. Adler, Michael F. F. Olson
Summary: Cytoskeleton organization and dynamics are regulated by post-translational modifications of key target proteins, including the phosphorylation of myosin light chain proteins by MRCK kinases. Compared to ROCK kinases, the contributions of MRCK kinases are less characterized due to the late discovery of selective inhibitors. The recent development of selective MRCK inhibitors has expanded the tools to study MRCK function and shown therapeutic benefits in cancer studies.
Article
Plant Sciences
Weikun Jing, Yonghong Li, Shuai Zhang, Xiaofeng Zhou, Junping Gao, Nan Ma
Summary: Aquaporins (AQPs), as an ancient superfamily protein, play a crucial role in maintaining fluid homeostasis in organisms across different environments. This review summarizes the recent findings on the involvement of AQPs in plant cell signal transduction and highlights the unresolved issues for future investigation.
HORTICULTURAL PLANT JOURNAL
(2023)
Article
Microbiology
Zhichao Jiang, Zijia Lin, Qi Gan, Pengju Wu, Xuemei Zhang, Yuanxi Xiao, Qunxin She, Jinfeng Ni, Yulong Shen, Qihong Huang
Summary: Protein phosphorylation and its recognition by forkhead-associated (FHA) domain proteins play important roles in signal transduction in mammals. However, the functions of FHA proteins, especially in DNA damage response (DDR), in prokaryotes and archaea are less known. By studying an FHA protein (SisArnA) from an archaeon, it was found that SisArnA and its phosphorylated partner SisArnB repress the transcription of pili genes and facilitate DNA exchange and repair. SisArnA also regulates the expression of several genes involved in DDR, suggesting that the FHA/phosphorylation module may be an important signal transduction pathway in archaeal DDR.
Article
Biochemistry & Molecular Biology
Yan Zhao, Hui Shi, Ying Pan, Mohan Lyu, Zhixuan Yang, Xiaoxia Kou, Xing Wang Deng, Shangwei Zhong
Summary: A study found that Ca2+ signaling in photosynthetic plants controls the nuclear translocation of photoreceptor phyB during the transition from darkness to light. Red light stimulates cytosolic Ca2+ increases via phyB, which are sensed by Ca2+-binding protein kinases CPK6 and CPK12. Upon Ca2+ activation, CPK6/12 directly interact with and phosphorylate photo-activated phyB at Ser80/Ser106 to initiate phyB nuclear import. This research uncovers a biochemical regulatory loop in phyB phototransduction and provides a paradigm for connecting ubiquitous Ca2+ increases to specific responses in sensory stimulus processing.
Article
Cardiac & Cardiovascular Systems
Jinhong Wei, Wenting Guo, Ruiwu Wang, John Paul Estillore, Darrell Belke, Yong-Xiang Chen, Alexander Vallmitjana, Raul Benitez, Leif Hove-Madsen, S. R. Wayne Chen
Summary: The physiological significance of PKA phosphorylation of RyR2 in the heart is still poorly understood. Recent structural studies have shown that the PKA phosphorylation site S2030 in RyR2 is located within a pathway that is important for the termination of Ca2+ release. We investigated the impact of S2030 mutations on Ca2+ release termination in cells and generated a mouse model to study the role of S2030 in a physiological setting.
CIRCULATION RESEARCH
(2023)
Review
Plant Sciences
Barkha Ravi, Christine H. Foyer, Girdhar K. Pandey
Summary: Reactive oxygen species (ROS) and calcium (Ca2+) signalling play important roles in plant growth, development, and defense. They function together with electric signals in directional cell-to-cell systemic signalling and even plant-to-plant communication. However, there is still limited understanding of the molecular mechanisms underlying the management of ROS and Ca2+ signals, as well as the synchronous and independent signaling in different cellular compartments. This review focuses on the proteins that may connect different pathways during abiotic stress responses, and highlights the crosstalk between ROS and Ca2+ pathways in cell signaling.
PLANT CELL AND ENVIRONMENT
(2023)
Review
Plant Sciences
Ambra S. Parmagnani, Massimo E. Maffei
Summary: This review summarizes recent advances in Ca2+ signaling upon herbivory and reviews the most recent Ca2+ imaging techniques and methods.
Article
Biochemistry & Molecular Biology
Francesca Aglialoro, Asena Abay, Nurcan Yagci, Minke A. E. Rab, Lars Kaestner, Richard van Wijk, Marieke von Lindern, Emile van den Akker
Summary: In this study, we found that PIEZO1 acts as a mechanosensor that, when transferred to orbital shaking, activates signaling cascades that are Ca2+-dependent, regulating various aspects of erythropoiesis. This suggests that there are direct physiological outcomes of mechanical stress on erythroblasts, as shown by the comparable activation of some Ca2+-dependent pathways induced by Yoda1 treatment and mechanical stress via orbital shaking.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Aziz M. Rangwala, Victoria R. Mingione, George Georghiou, Markus A. Seeliger
Summary: Phosphorylation plays a vital role in regulating biological processes, and protein kinases have been extensively studied for their involvement in human health and disease. Some kinases possess additional catalytic functions in addition to phosphotransferase activity, while others have lost their catalytic activity completely. In this study, we analyzed the UniProtKB database for bifunctional protein kinases and focused on those crucial for bacterial and human cellular homeostasis. These kinases have diverse functional roles in environmental sensing, metabolic regulation, immune-host defense, and cell cycle control. This article explores their dual catalytic activities and their contributions to disease pathogenesis.
Article
Chemistry, Multidisciplinary
Matthew M. Wootten, Bryden A. F. Le Bailly, Sofja Tshepelevitsh, Ivo Leito, Jonathan Clayden
Summary: This study reports a molecular system that selectively protonates chiral anionic ligands of different basicity based on the pH of the solution. The system utilizes a cationic circular dichroism (CD) reporter complex to respond to anion binding by adopting one of two alternative enantiomeric conformations. By altering the pH through sequential addition of base and acid, the system achieves cyclic switching between three ligand-bound states. Furthermore, by appending an aminoisobutyric acid oligomer to the binding site, the pH-dependent conformational response can be transduced into a programmable response that is detectable through remote NMR spectroscopy.
Article
Biochemistry & Molecular Biology
Jisun Hwang, Bohee Jang, Ayoung Kim, Yejin Lee, Joonha Lee, Chungho Kim, Jinmahn Kim, Kyeong Min Moon, Kyuhyung Kim, Ram Wagle, Young-Han Song, Eok-Soo Oh
Summary: The transmembrane domain (TMD) of syndecan-2 specifically regulates receptor cytoplasmic domain function and downstream signaling events controlling cell behavior, as shown through in vivo invertebrate models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Harumi Kagiwada, Takayuki Kiboku, Hitomi Matsuo, Masashi Kitazawa, Kazuhiko Fukui, Katsuhisa Horimoto
Summary: Cellular protein phosphorylation is crucial for signal transduction, and the newly developed phosphorylation array system effectively assesses the activity of signaling pathways perturbed by external stimuli, offering a new approach to understanding intracellular signal transduction mechanisms.
Review
Plant Sciences
Houqing Zeng, Qiuqing Zhu, Peiguo Yuan, Yan Yan, Keke Yi, Liqun Du
Summary: Plants have developed mechanisms using calcium as signals to respond to biotic stresses, with calmodulins (CaMs) and calmodulin-like proteins (CMLs) acting as important calcium sensors. The coordination between calcium transients, CaMs/CMLs, and target proteins is crucial for plant responses. This review focuses on the involvement of CaMs/CMLs in plant responses to various biotic stresses and discusses future directions in understanding the signaling pathways. The application of this knowledge in crop cultivation can improve resistance to biotic stresses and ensure food production.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Gimena dos Santos, Micah R. Rogel, Margaret A. Baker, James R. Troken, Daniela Urich, Luisa Morales-Nebreda, Joseph A. Sennello, Mikhail A. Kutuzov, Albert Sitikov, Jennifer M. Davis, Anna P. Lam, Paul Cheresh, David Kamp, Dale K. Shumaker, G. R. Scott Budinger, Karen M. Ridge
NATURE COMMUNICATIONS
(2015)
Article
Cell Biology
Alexandra V. Andreeva, Mikhail A. Kutuzov, Vsevolod A. Tkachuk, Tatyana A. Voyno-Yasenetskaya
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2009)
Article
Cell Biology
Mikhail A. Kutuzov, Alexandra V. Andreeva, Nelly Bennett
CELLULAR SIGNALLING
(2012)
Review
Genetics & Heredity
Mikhail A. Kutuzov, Alexandra V. Andreeva
FUNCTIONAL & INTEGRATIVE GENOMICS
(2012)
Review
Oncology
Alexandra V. Andreeva, Mikhail A. Kutuzov
GENES CHROMOSOMES & CANCER
(2010)
Review
Parasitology
Alexandra V. Andreeva, Mikhail A. Kutuzov
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2008)
Article
Biochemistry & Molecular Biology
Mikhail A. Kutuzov, Nelly Bennett, Alexandra V. Andreeva
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2010)
Article
Cell Biology
Alexandra V. Andreeva, Jingyan Han, Mikhail A. Kutuzov, Jasmina Profirovic, Vsevolod A. Tkachuk, Tatyana A. Voyno-Yasenetskaya
JOURNAL OF CELLULAR PHYSIOLOGY
(2010)
Review
Biochemistry & Molecular Biology
Mikhail A. Kutuzov, Alexandra V. Andreeva
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
(2008)
Article
Biology
Enas Abu-Shah, Philippos Demetriou, Stefan Balint, Viveka Mayya, Mikhail A. Kutuzov, Omer Dushek, Michael L. Dustin
Article
Biology
Johannes Pettmann, Anna Huhn, Enas Abu Shah, Mikhail A. Kutuzov, Daniel B. Wilson, Michael L. Dustin, Simon J. Davis, P. Anton van der Merwe, Omer Dushek
Summary: The study found that while TCR discrimination is enhanced compared to conventional cell-surface receptors, it is not perfect. Human T cells can respond to pMHC with affinities as low as K-D around 1 mM, and the kinetic proofreading mechanism explains this extraordinary sensitivity.
Article
Biology
Michael Barton, Stuart A. MacGowan, Mikhail A. Kutuzov, Omer Dushek, Geoffrey John Barton, P. Anton van der Merwe
Summary: The study analyzed the effects of mutations in the Spike protein of SARS-CoV-2 on its interaction with ACE2, finding that most mutations increased affinity with ACE2. Two ACE2 mutations were also found to enhance binding with the Spike protein.
Article
Biochemical Research Methods
Stuart MacGowan, Michael Barton, Mikhail Kutuzov, Omer Dushek, P. Anton van der Merwe, Geoffrey Barton
Summary: This study investigated the impact of ACE2 gene variants on SARS-CoV-2 infection. The research found that certain ACE2 variants can reduce the binding capability between the virus and host cells, thus protecting cells from infection, while other variants enhance the binding capability, potentially leading to wider viral spreading across tissues and cell types.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Johannes Pettmann, Lama Awada, Bartosz Rozycki, Anna Huhn, Sara Faour, Mikhail Kutuzov, Laurent Limozin, Thomas R. Weikl, P. Anton van der Merwe, Philippe Robert, Omer Dushek
Summary: T cells discriminate between self and foreign peptide-MHC complexes based on TCR/pMHC off-rate and generate forces during this process. We found that weaker TCR/pMHC interactions are more resistant to mechanical force than stronger interactions. This suggests that reducing forces on the TCR/pMHC interaction improves antigen discrimination, possibly through involvement of adhesion receptors CD2 and LFA-1.
