Article
Immunology
Adriana Dominguez-Flores, Gloria M. Rodriguez Lopez, Rodolfo Soria-Castro, Ruben Lopez-Santiago, Octavio Rodriguez-Cortes, Sonia M. Perez-Tapia, Alma D. Chavez-Blanco, Sergio Estrada-Parra, Raul Flores-Mejia, Rommel Chacon-Salinas
Summary: Brucella abortus, a Gram-negative bacteria, is a major cause of brucellosis in animals and humans. This study analyzed the role of innate immune receptors TLR2 and TLR4 in mast cell activation during B. abortus infection. The results showed that B. abortus induced the synthesis of several cytokines and activated key cell signaling molecules in mast cells. Blockade of TLR2 and TLR4 receptors decreased the release of TNF-alpha and IL-6 by mast cells in response to B. abortus. Overall, mast cells are activated by B. abortus and may contribute to the inflammatory response during the initial phase of the infection.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Hai Hu, Guangdong Zhang, Mingxing Tian, Xiang Guan, Yi Yin, Chan Ding, Shengqing Yu
Summary: In this study, a comparative transcriptome assay of macrophages infected with Brucella was performed to investigate the differentially expressed genes related to the pathogenesis of the bacterium. The results showed that Brucella infection led to host oxidative stress and ferroptosis, especially during the infection of the rough mutant RB14. Investigating how Brucella manipulates oxidative stress and ferroptosis in its host will be helpful to clarify the pathogenicity of Brucella.
Article
Agriculture, Dairy & Animal Science
Kaitlyn M. Sarlo Davila, Paola Boggiatto, Steven Olsen, John D. Lippolis, Brian A. Crooker, Ellie J. Putz
Summary: This study compares the transcriptomic response of Holsteins with different genetic backgrounds to bacterial challenges, and suggests that contemporary cows, genetically selected for milk production traits, may have reduced immune function.
Article
Immunology
Mahdieh Sarmadi, Azam Gheibi, Hossein Khanahmad, Mohammad Reza Khorramizadeh, Seyed Hossein Hejazi, Noushin Zahedi, Hamidreza Mianesaz, Khosrow Kashfi
Summary: In this study, we transformed Listeria monocytogenes genes into Brucella abortus RB51 strain, enhancing its cytotoxicity and immune response. This recombinant strain shows potential as a safer and more effective candidate vaccine against B. abortus.
Review
Microbiology
Idoia Buldain, Leire Martin-Souto, Aitziber Antoran, Maialen Areitio, Leire Aparicio-Fernandez, Aitor Rementeria, Fernando L. Hernando, Andoni Ramirez-Garcia
Summary: Infections caused by opportunistic pathogens Scedosporium/Lomentospora are increasing, posing challenges in diagnosis and treatment in the clinic. Understanding the immune response against these fungi is crucial for developing effective strategies. While progress has been made, many aspects remain unclear and further research is needed to elucidate the immune response to Scedosporium/Lomentospora.
Article
Microbiology
Maria Ines Marchesini, Ansgar Poetsch, Leticia Soledad Guidolin, Diego J. Comerci
Summary: Rhomboids are intramembrane serine proteases highly conserved in all life domains. The study showed that Brucella abortus encodes an active rhomboid protease capable of cleaving heterologous substrates. Deletion of rhomboid had no significant impact on B. abortus virulence, but overexpression had negative effects on growth.
Article
Veterinary Sciences
Dongjie Sun, Yufu Liu, Xiaowei Peng, Hao Dong, Hui Jiang, Xuezheng Fan, Yu Feng, Jiali Sun, Kun Han, Qiang Gao, Jianrui Niu, Jiabo Ding
Summary: The process of intracellular proteolysis via ATP-dependent proteases is biologically conserved. The ATP-dependent Clp protease is associated with stress responses and bacterial virulence in various pathogenic bacteria. A Brucella abortus 2308 strain with a ?clpP mutation was constructed to investigate the function of ClpP peptidase. The results revealed that the ?clpP mutant strain exhibited impaired growth in TSB medium and increased sensitivity to various stress conditions. Additionally, deletion of clpP significantly affected Brucella virulence and led to perturbations in gene and protein expression related to energy production, cell wall/membrane/envelope biogenesis, carbohydrate transport, and metabolism.
VETERINARY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Ahmed Al-Qahtani, Mohammed N. Al-Ahdal, Saad Alkahtani
Summary: Leishmania species cause a variety of diseases ranging from moderate to severe, impacting morbidity and mortality. The human innate immune system deals with Leishmania through the complement system, particularly the classical pathway, which is activated first due to a lag phase before the alternative pathway activation. Recognition molecule C1q binds to soluble leishmania antigens (SLA) and activates the classical pathway, leading to downregulation of cytokine and chemokine responses. This highlights the importance of potent adjuvants in SLA-mediated vaccine strategies.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Magdalena Druszczynska, Magdalena Godkowicz, Jakub Kulesza, Sebastian Wawrocki, Marek Fol
Summary: Cytokine receptors play a critical role in regulating immune response against mycobacteria by recognizing and binding specific cytokines and activating intracellular signaling pathways. Mutations in cytokine receptor genes may lead to increased susceptibility to mycobacteria and impaired immune surveillance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
J. Barthes, P. Lagarrigue, V. Riabov, G. Lutzweiler, J. Kirsch, C. Muller, E. -J. Courtial, C. Marquette, F. Projetti, J. Kzhyskowska, P. Lavalle, N. E. Vrana, A. Dupret-Bories
Summary: A new cytokine cocktail and hydrogel were designed to control immune responses, promote tissue integration, and increase the survival rate of implants. In vivo experiments demonstrated that implants coated with this hydrogel could reduce inflammatory reactions and significantly improve animal survival rates.
Article
Multidisciplinary Sciences
Supratim Pradhan, Debolina Manna, Subir Karmakar, Manoj Kumar Singh, Arijit Bhattacharya, Budhaditya Mukherjee, Joydeep Paul, Joydeep Paul
Summary: Immunotherapeutic strategies are important for the treatment of visceral leishmaniasis (VL) due to the resistance against existing chemotherapy drugs. In this study, two synthesized flavones with terminal galactose, Q-gal and K-gal, were found to induce host protective Th1 response against antimony-resistant and antimony-susceptible Leishmania donovani in both in vitro and in vivo models. The treatment with Q-gal and K-gal also showed significant antileishmanial efficacy by reducing the parasite burden and promoting Th1 cytokine production via TLR4. Moreover, these compounds were able to generate ROS in macrophages to directly kill intracellular parasites.
Article
Biochemistry & Molecular Biology
Jeremy Verbeke, Youri Fayt, Lisa Martin, Oya Yilmaz, Jaroslaw Sedzicki, Angeline Reboul, Michel Jadot, Patricia Renard, Christoph Dehio, Henri-Francois Renard, Jean-Jacques Letesson, Xavier De Bolle, Thierry Arnould
Summary: The facultative intracellular pathogen Brucella abortus interacts with host cell organelles, particularly the mitochondria, for replication. This study demonstrates that B. abortus induces fragmentation of the mitochondrial network, mitophagy, and the formation of mitochondrial Brucella-containing vacuoles during late stages of cellular infection. This process is dependent on the expression of the mitophagy receptor BNIP3L, which is regulated by iron-dependent stabilization of hypoxia-inducible factor 1 alpha. BNIP3L-mediated mitophagy is advantageous for bacterial exit from the host cell. These findings highlight the intricate relationship between Brucella trafficking and the mitochondria during host cell infection.
Article
Veterinary Sciences
Jeyhun Aliyev, Mahnur Alakbarova, Aytan Garayusifova, Asaf Omarov, Saida Aliyeva, David Fretin, Jacques Godfroid
Summary: The study in the Ganja region of Azerbaijan found that despite the vaccination program in small ruminants, spill-over events of Brucella melitensis from small ruminants to cattle have occurred. Cattle are primarily infected with Brucella abortus, leading to the implementation of a bovine brucellosis program. Cattle in the region should be considered as a potential source of Brucella abortus and Brucella melitensis for humans.
BMC VETERINARY RESEARCH
(2022)
Article
Immunology
Tianyi Zhao, Zedan Zhang, Yitao Li, Zhihua Sun, Liangbo Liu, Xingmei Deng, Jia Guo, Dexin Zhu, Shuzhu Cao, Yingjin Chai, Usevich Vera Nikolaevna, Suleimenov Maratbek, Zhen Wang, Hui Zhang
Summary: This study investigated the mechanism of Brucella infection in modulating macrophage phenotype. The results showed that Brucella infection induced macrophage phenotypic switch and inflammatory response in a time-dependent manner. The transcription factor NF-kappa B and its target gene Gls played an important role in regulating macrophage phenotypic transition.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
E. Maurizio, M. D. Trangoni, U. A. Rossi, M. Dunleavy, C. Colato, C. A. Rossetti
Summary: Caprine brucellosis is a globally distributed disease that affects goat reproduction, with the causative agent Brucella melitensis having zoonotic potential. Research on innate immunity in goats shows some individuals can resist Brucella infection, with certain macrophages showing enhanced ability to restrict intracellular bacterial growth. Further studies on humoral and cellular immunity reveal a correlation between natural antibodies and cross-reacting antibodies in response to Brucella infection.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Wen Tang, Le Liang, Rulong Li, Yongdong Xie, Xiaomei Li, Huanxiu Li, Lijin Lin, Zhi Huang, Bo Sun, Guochao Sun, Lihua Tu, Yi Tang
Summary: This study found that exogenous melatonin could promote the growth and development of lettuce under cadmium stress. Melatonin application increased biomass and photosynthetic rate, and decreased oxidative damage in lettuce. In addition, melatonin regulated the transport of cadmium, reducing its accumulation in the shoots. These findings suggest that melatonin may have potential applications in crops threatened by heavy metals.
ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY
(2023)
Article
Computer Science, Cybernetics
Fei Lyu, Huijing Li, Qiang Fu, Yujie Liu, Jin Huang, Zhigang Deng
Summary: This paper investigates children's performance on mid-air gesture interactions in different spatial constraints and the age effect on such interaction scenarios. Based on the experiments, an improved design for UI menus based on mid-air gesture interaction for children is proposed.
INTERNATIONAL JOURNAL OF HUMAN-COMPUTER STUDIES
(2023)
Article
Engineering, Electrical & Electronic
Yujie Chen, Yan Wang, Jianan Hu, Tunan Lv, Hongbin Yu
Summary: A new strategy based on directly patterning the flat photoacoustic source is proposed to address the existing issues of laser-generated focused ultrasound. A planar photoacoustic transmitter is constructed using a chromium mask with transparent rings as the substrate, and its effects on focusing characteristics are experimentally explored for the first time. The transmitter demonstrates the capability to provide a focused acoustic field with high pressure, small lateral size, and large focal depth, benefiting applications requiring large ultrasound working distance.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Article
Psychology, Multidisciplinary
Yuyang Zhang, Jing Yang, Zhisheng (Edward) Wen
Summary: This study examines the impact of learners' working memory capacity (WMC) on the facilitation effect of an instructor's presence during video lectures. The results show that the presence of the instructor significantly improves comprehension performance, especially for learners with low WMC. These learners pay more attention to the instructor's face picture and talking head. The findings highlight the importance of the instructor's presence as a social cue in video lectures.
JOURNAL OF INTELLIGENCE
(2023)
Article
Nanoscience & Nanotechnology
Xianshuo Wei, Qijun Wu, Lian Chen, Ye Sun, Lin Chen, Chunmei Zhang, Shanshan Li, Chunxin Ma, Shaohua Jiang
Summary: This study develops an anisotropic bilayer hydrogel actuator with multiple responsiveness, multiple remote actuation, high strength, fast responsiveness, and programmable complex deformation. The Fe3O4/P-(NIPAM-ABP) hydrogel actuator demonstrates excellent mechanical strength and response speed, aided by the fibrosis and porosity of the hydrogel. Moreover, the actuator benefits from the photothermal properties and magnetic conductivity of Fe3O4 nanoparticles, as well as the electrical conductivity of Ag microsheets. The actuator shows both short-range and remote actuation capabilities, making it suitable for complex bioinspired systems.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Geriatrics & Gerontology
Pingting Zhong, Shaoying Tan, Zhuoting Zhu, Junyao Zhang, Shida Chen, Wenyong Huang, Mingguang He, Wei Wang
Summary: This multicountry prospective study aimed to assess the correlation between cognitive signatures and the risk of diabetic vascular complications and mortality. Two cohorts were used, including 27,773 diabetics from the UK Biobank (UKB) and 1307 diabetics from the Guangzhou Diabetic Eye Study (GDES) cohort. In the UKB group, a reduction in brain gray matter volume and impaired memory were associated with higher risks of myocardial infarction, end-stage renal disease, and diabetic retinopathy. Impaired reaction was associated with increased risk of mortality and end-stage renal disease.
AMERICAN JOURNAL OF GERIATRIC PSYCHIATRY
(2023)
Article
Marine & Freshwater Biology
Dingrui Liu, Zhuowei Zhang, Yikun Song, Jiayu Yang, Yuyou Lu, Wenjie Lai, Ziyi Wu, Dandan Zhao, Haoran Lin, Yong Zhang, Jin Zhang, Shuisheng Li
Summary: Grass carp, an important freshwater fish species in China, showed great adaptability to low salinity but exhibited detrimental effects when exposed to high salinity. Changes in salinity also influenced the gut microbiota of grass carp, ultimately affecting their growth.
AQUATIC TOXICOLOGY
(2023)
Article
Oncology
Zhaodong Fei, Huiling Hong, Ting Xu, Yiying Xu, Jiawei Chen, Xiufang Qiu, Jianming Ding, Ye Feng, Chaoxiong Huang, Li Li, Mengying Li, Chuanben Chen
Summary: This retrospective study identified the main risk factors for metachronous metastatic nasopharyngeal carcinoma (NPC) and estimated their weight in different time periods. It was found that most metastasis occurred within the first 2 years after treatment, with early metastasis mainly influenced by tumor-related factors and late metastasis affected by patient-related factors.
Article
Cell Biology
Changling Xu, Jieyu Qi, Xiaojie Hu, Liyan Zhang, Qiuhan Sun, Nianci Li, Xin Chen, Fangfang Guo, Peina Wu, Yi Shi, Renjie Chai
Summary: Upregulating Rps14 expression enhances the ability of hair cell progenitors to proliferate and differentiate into hair cells, promoting hair cell regeneration. This study reveals the potential role of Rps14 in driving hair cell regeneration in mammals.
CELL PROLIFERATION
(2023)
Article
Pharmacology & Pharmacy
Yiwei He, Tiancheng Xu, Tingting Ding, Yuanchuan Gong, Hui Zeng, Zihan Xi, Yuanxin Ye, Ziyang Song, Ting Pan, Zhewei Zhang, Qian Ma, Lihua Li, Yuqing Zhang, Guoliang Shao
Summary: We developed multimodal imaging nano-assembled microspheres (NAMs) that can be visualized under CT/MR and help determine the location of embolic microspheres during postoperative review. Compared with commercially available DC Bead and CalliSpheres microspheres, NAMs showed similarities in drug loading capacity, drug release profiles, diameter variation, and morphological characteristics. Therefore, NAMs have a promising application prospect in transcatheter arterial chemoembolization treatment of hepatocellular carcinoma.
Article
Medicine, General & Internal
Shiping He, Ruofan Li, Shangyi Jin, Yanhong Wang, Hongbin Li, Xinwang Duan, Lili Pan, Lijun Wu, Yongfu Wang, Yan Zhang, Zhenbiao Wu, Jing Li, Yunjiao Yang, Xinping Tian, Xiaofeng Zeng
Summary: This study analyzed the factors associated with relapse in Takayasu arteritis (TAK) patients and developed a prediction model for relapse. The prediction model accurately stratifies patients' relapse risk and can assist in clinical decision-making.
EUROPEAN JOURNAL OF INTERNAL MEDICINE
(2023)
Article
Energy & Fuels
Congbiao Chen, Bo Hou, Yan Liu, Litao Jia, Zhongyi Ma, Jungang Wang, Qiang Wang, Debao Li
Summary: The hcp-Co@Co2C catalysts with rich Co-Co2C interface and strong synergy between hcp-Co and Co2C were prepared by partial carbonization conversion of hcp-Co, compared with fcc-Co. The degree of carbonization conversion was easy to control by using ethyne as carbon source. The prepared hcp-Co@Co2C catalysts exhibited high selectivity of higher alcohols, owing to the rich interface and strong synergy between hcp-Co and Co2C.
Article
Pharmacology & Pharmacy
Xue Gao, Xiuxiu Cong, He Li, Kuirong Mao, Haorui Wang, Guojiao Lin, Jialiang Wang, Feiqi Liu, Ge Zhu, Huizhu Tan, Yuning Zhang, Liguang Sun, Yong-Guang Yang, Tianmeng Sun
Summary: In this study, researchers explored the potential of 1 alpha, 25-Dihydroxyvitamin D3 (VD3) to induce tolerogenic dendritic cells (tDCs) in a mouse model of experimental autoimmune encephalomyelitis (EAE). They prepared a PLGA-PEG-based nano-drug delivery system to co-deliver VD3 and myelin-oligodendrocyte glycoprotein (MOG) peptides. The study found that the delivery system significantly enhanced the level of antigen-specific Tregs in vivo and improved the symptoms of EAE mice.
ADVANCED THERAPEUTICS
(2023)
Article
Computer Science, Artificial Intelligence
Xing Wei, Bin Wen, Fan Yang, Yujie Liu, Chong Zhao, Di Hu, Hui Luo
Summary: Unsupervised domain adaptation aims to transfer knowledge from labeled source domain to unlabeled target domain for improving target domain's classification performance. However, existing methods only focus on domain alignment in contrastive learning, neglecting the classification task, leading to suboptimal solutions. In this paper, we propose Task-oriented Contrastive Learning for Unsupervised Domain Adaptation (TOCL) to address the inconsistency problem. Our method performs feature weighting and extracts task-related features from source domain images after data augmentation for contrastive learning. Additionally, a delimitation discriminator is introduced to detect and minimize the output difference between two types of data-augmented samples in the target domain. Extensive experiments on public datasets demonstrate the effectiveness and adaptability of TOCL in improving classification accuracy.
EXPERT SYSTEMS WITH APPLICATIONS
(2023)
Article
Chemistry, Physical
Junmeng Zhang, Jianmin Lu, Panzhe Hou, Yujie Liu, Zixuan Li, Peipei Lu, Guangyu Wen, Lihu Liu, Huiyuan Sun
Summary: Highly ordered TiO2 nanotubes with large surface-volume ratios were prepared and subjected to annealing in different atmospheres. The annealed samples exhibited low-field ferromagnetism and high-field diamagnetism at room temperature. The effects of annealing conditions and induced morphology changes on the optical and magnetic properties of the nanotubes were revealed. The research provides valuable insights for expanding the application of TiO2 nanotubes.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
