Article
Biotechnology & Applied Microbiology
Rui-Men Zhang, Yu Pan, Chao-Xia Zou, Qiang An, Juan-Ru Cheng, Peng-Ju Li, Zi-Hua Zheng, Yan Pan, Wan-You Feng, Su-Fang Yang, De-Shun Shi, Ying-Ming Wei, Yan-Fei Deng
Summary: This study explores the role of circUBE2Q2 in muscle growth and development. The results show that circUBE2Q2 acts as a sponge for miR-133a, promoting the differentiation of muscle stem cells and inhibiting proliferation. This finding provides new insights into the mechanisms by which mRNA and non-coding RNAs regulate skeletal muscle growth and development.
Article
Biochemistry & Molecular Biology
Shuailong Zheng, Li Li, Helin Zhou, Xujia Zhang, Xiaoli Xu, Dinghui Dai, Siyuan Zhan, Jiaxue Cao, Jiazhong Guo, Tao Zhong, Linjie Wang, Hongping Zhang
Summary: This study identifies a novel circular RNA, circTCF4, which is specifically expressed during the development of the longest dorsal muscle in goats and is enriched in muscle tissue. The study demonstrates that circTCF4 inhibits the proliferation and differentiation of MuSCs and affects muscle differentiation by influencing multiple signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Di Sun, Jiaqi An, Zixu Cui, Jiao Li, Ziwei You, Chang Lu, Yang Yang, Pengfei Gao, Xiaohong Guo, Bugao Li, Chunbo Cai, Guoqing Cao
Summary: This study identifies a novel circRNA, circCSDE1, that is highly expressed in skeletal muscle. Functional studies show that circCSDE1 promotes proliferation and inhibits myoblast differentiation, while miR-21-3p has opposite effects. Dual-luciferase reporter assay confirms that circCSDE1 targets miR-21-3p directly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Eun Ju Lee, Sibhghatulla Shaikh, Mohammad Hassan Baig, So-Young Park, Jeong Ho Lim, Syed Sayeed Ahmad, Shahid Ali, Khurshid Ahmad, Inho Choi
Summary: This study reports the potential of novel peptides MIF1 and MIF2 derived from MSTN in enhancing myogenesis and reducing adipogenesis. Modified MIF1 and MIF2 showed better cell proliferation and differentiation compared to non-modified peptides. Pretreatment of mice with (Ac)-MIF1 or (Ac)-MIF2-(NH2) promoted muscle regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Jakaria Jakaria, Wenny Ladhunka Nur Aliyya, Riyadi Ismail, Sri Yuni Siswanti, Mokhamad Fakhrul Ulum, Rudy Priyanto
Summary: This study investigated single-nucleotide polymorphisms (SNPs) and 11-bp deletions in the coding region of the MSTN gene in Belgian Blue, Peranakan Ongole, and their crossbred cattle. Although four SNPs and an 11-bp deletion were found, they were not able to differentiate between normal and double-muscled phenotypes accurately.
Article
Biochemistry & Molecular Biology
Mingfa Ling, Xumin Lai, Lulu Quan, Fan Li, Limin Lang, Yiming Fu, Shengchun Feng, Xin Yi, Canjun Zhu, Ping Gao, Xiaotong Zhu, Lina Wang, Gang Shu, Qingyan Jiang, Songbo Wang
Summary: This study investigates the effects of VEGFB and VEGFR1 on fat deposition and skeletal muscle growth. The results suggest that knocking down VEGFB promotes adipose tissue browning and skeletal muscle proliferation, while knocking down VEGFR1 promotes adipose tissue browning but inhibits skeletal muscle differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mira Oh, Seo-Young Kim, SeonJu Park, Kil-Nam Kim, Seung Hyun Kim
Summary: Chinese chive contains compounds that can promote skeletal muscle cell proliferation, with newly isolated flavonoid and 5-aminouridine showing positive effects on skeletal muscle growth by up-regulating PI3K/Akt/mTOR pathways. Additionally, the isolated compound 1 down-regulated the Smad pathways, which are negative regulators of skeletal muscle growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Haorong He, Huadong Yin, Xueke Yu, Yao Zhang, Menggen Ma, Diyan Li, Qing Zhu
Summary: This study demonstrated that PDLIM5 can promote proliferation and differentiation of chicken skeletal muscle satellite cells through the p38-MAPK pathway. These findings suggest that PDLIM5 may play a role in the growth and development of chicken skeletal muscle.
Article
Multidisciplinary Sciences
Dhanushika Ratnayake, Phong D. Nguyen, Fernando J. Rossello, Verena C. Wimmer, Jean L. Tan, Laura A. Galvis, Ziad Julier, Alasdair J. Wood, Thomas Boudier, Abdulsalam I. Isiaku, Silke Berger, Viola Oorschot, Carmen Sonntag, Kelly L. Rogers, Christophe Marcelle, Graham J. Lieschke, Mikael M. Martino, Jeroen Bakkers, Peter D. Currie
Summary: Through muscle injury models in zebrafish, it has been found that specific macrophage populations 'dwell' within the injury site, providing a transient niche for stem cell proliferation. The signals released by these macrophages directly stimulate stem cell proliferation, governing the repair process.
Article
Cell Biology
Yilei Li, Xiaoli Sun, Yun Bai, Yunyan Ji, Huawei Ren, Xiuju Yu, Yi Yan, Xiaoyan He, Yanjun Dong, Liping Zhang, Xiaomao Luo, Haidong Wang
Summary: Recent study showed that fertilized egg yolk extract (FEYE) can promote skeletal muscle development by inhibiting the expression of muscle atrophy genes, and stimulate the browning reaction of white adipocytes.
Review
Cell Biology
Ming-Ming Chen, Yi-Ping Zhao, Yue Zhao, Shou-Long Deng, Kun Yu
Summary: Myostatin, a member of the TGF-β superfamily, negatively regulates skeletal muscle growth and development through autocrine or paracrine signaling, with mutations leading to increased muscle mass. It regulates myogenic differentiation, protein synthesis, and oxidative stress in skeletal muscle.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Huimin Zhen, Jiyuan Shen, Jiqing Wang, Yuzhu Luo, Jiang Hu, Xiu Liu, Shaobin Li, Zhiyun Hao, Mingna Li, Bingang Shi, Yuanhua Gu
Summary: circ_003628 is a highly expressed circular RNA in goat skeletal muscle tissue, and it affects muscle growth and development by promoting the proliferation and differentiation of goat skeletal muscle satellite cells.
Article
Cell Biology
Bingyuan Wang, Jiankang Guo, Mingrui Zhang, Zhiguo Liu, Rong Zhou, Fei Guo, Kui Li, Yulian Mu
Summary: The study revealed that IDE regulates the proliferation and apoptosis of PSMSCs via the MSTN/MYOD pathway, playing a crucial role in porcine skeletal muscle development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Bing Fu, Jinzeng Yang, Yan Yang, Jun Xia, Yinglin He, Qing Wang, Huihong Zhao, Huirong Yang
Summary: This study investigated the role of myostatin in E. coioides and demonstrated the positive effects of autologous nucleic acid vaccine on muscle growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Jacqueline A. Larouche, Mahir Mohiuddin, Jeongmoon J. Choi, Peter J. Ulintz, Paula Fraczek, Kaitlyn Sabin, Sethuramasundaram Pitchiaya, Sarah J. Kurpiers, Jesus Castor-Macias, Wenxuan Liu, Robert Louis Hastings, Lemuel A. Brown, James F. Markworth, Kanishka De Silva, Benjamin Levi, Sofia D. Merajver, Gregorio Valdez, Joe Chakkalakal, Young C. Jang, Susan Brooks, Carlos A. Aguilar
Summary: The relationship between MuSCs and neuromuscular innervation has been examined through severe neuromuscular trauma in a transgenic mouse model, showing a reduction in localized engraftment behavior in aging and degenerative muscle conditions. Genetic rescue of motor neurons in a mouse model restored MuSC ability to engraft near the neuromuscular junction, indicating a potential therapeutic avenue for age-related muscle degeneration. Single cell RNA-sequencing revealed molecular distinctions in MuSCs responding to synaptic perturbations, suggesting unique features of MuSCs in addressing muscle aging and stress-related challenges.
Review
Microbiology
A. K. M. Humayun Kober, Muhammad Shahid Riaz Rajoka, Hafiza Mahreen Mehwish, Julio Villena, Haruki Kitazawa
Summary: In the past decade, the use of probiotics as feed supplements in animal production has significantly increased due to the ban on antibiotic growth promoters. Probiotics have been suggested to improve the health, immunity, growth performance, nutritional digestibility, and intestinal microbial balance of livestock. Furthermore, they can help balance the beneficial microbial population and turnover in animals by stimulating the host immune response and excluding potentially pathogenic bacteria.
Article
Biochemistry & Molecular Biology
Mitsuaki Sobajima, Masato Miyake, Yoshimasa Hamada, Kazue Tsugawa, Miho Oyadomari, Ryota Inoue, Jun Shirakawa, Hiroshi Arima, Seiichi Oyadomari
Summary: The stress-inducible transcription factor ATF4 is crucial for the survival and identity of beta-cells during stress conditions. Its role in glucose-stimulated insulin secretion (GSIS) was investigated using beta-cell-specific Atf4 knockout mice. The results showed that ATF4 is essential for insulin production and glucose-stimulated ATP and cAMP production, contributing to the multifaceted regulatory process in GSIS.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Endocrinology & Metabolism
Domenico Accili, Wen Du, Takumi Kitamoto, Taiyi Kuo, Wendy McKimpson, Yasutaka Miyachi, Maria Mukhanova, Jinsook Son, Liheng Wang, Hitoshi Watanabe
Summary: Research on the etiology and treatment of diabetes has made significant progress. However, the number of patients achieving glycemic control targets has not increased, mainly due to the restoration of insulin sensitivity and the reversal of pancreatic beta cell failure. This review aims to integrate research advances in transcriptional regulation of insulin action and the pathophysiology of beta cell dedifferentiation, exploring the potential impact on prospects of a durable cure for patients suffering from type 2 diabetes.
DIABETOLOGY INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Yuka Inaba, Emi Hashiuchi, Hitoshi Watanabe, Kumi Kimura, Yu Oshima, Kohsuke Tsuchiya, Shin Murai, Chiaki Takahashi, Michihiro Matsumoto, Shigetaka Kitajima, Yasuhiko Yamamoto, Masao Honda, Shun-ichiro Asahara, Kim Ravnskjaer, Shin-ichi Horike, Shuichi Kaneko, Masato Kasuga, Hiroyasu Nakano, Kenichi Harada, Hiroshi Inoue
Summary: Aggravation of liver steatosis shifts the mode of hepatocellular death from apoptosis to necroptosis, and the transcription factor ATF3 regulates this shift through the induction of RIPK3. ATF3 acts as a master regulator in this shift by inducing RIPK3 expression. The frequency of hepatocytes expressing ATF3 or RIPK3 is correlated with hepatocellular damage in non-alcoholic steatohepatitis (NASH).
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Ayelen Baillo, Julio Villena, Leonardo Albarracin, Mikado Tomokiyo, Mariano Elean, Kohtaro Fukuyama, Sandra Quilodran-Vega, Silvina Fadda, Haruki Kitazawa
Summary: The immunobiotic potential of L. plantarum CRL681 and CRL1506 was studied in this research. The results showed that both strains could induce the expression of IL-6 and IL-8 in intestinal epithelial cells and enhance the levels of IFN-gamma and TNF-alpha in mice. In addition, these strains could modulate the expression of inflammatory cytokines and chemokines, reduce the pathogen counts, and prevent its spread to other organs. L. plantarum CRL1506 was found to be more efficient in modulating mucosal immunity than CRL681.
Article
Biochemistry & Molecular Biology
Stefania Dentice Maidana, Mariano Elean, Kohtaro Fukuyama, Yoshiya Imamura, Leonardo Albarracin, Sudeb Saha, Yoshihito Suda, Shoichiro Kurata, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: An increase in the isolation of hypermucoviscous carbapenem-resistant K. pneumoniae with sequence type 25 (ST25) was observed in hospitals in Norwest Argentina. In this study, it was found that the ST25 strains of K. pneumoniae can invade human intestinal epithelial cells and induce moderate inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Ramiro Ortiz Moyano, Fernanda Raya Tonetti, Kohtaro Fukuyama, Mariano Elean, Mikado Tomokiyo, Yoshihito Suda, Vyacheslav Melnikov, Haruki Kitazawa, Julio Villena
Summary: Cp and BLPs can stimulate alveolar macrophages and enhance the humoral immune response induced by the commercial pneumococcal vaccine. Mice immunized with Cp + PCV or BLPs + PCV showed higher levels of specific antibodies and lower levels of pneumococcal cell counts and lung damage compared to the control group. These findings demonstrate the potential of Cp and BLPs as adjuvants to enhance the immune response against respiratory pathogens.
Article
Microbiology
Stefania Dentice Maidana, Yoshiya Imamura, Mariano Elean, Leonardo Albarracin, Keita Nishiyama, Yoshihito Suda, Shoichiro Kurata, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: Orally administered Lacticaseibacillus rhamnosus CRL1505 enhances respiratory immunity, providing protection against respiratory viruses and Streptococcus pneumoniae. However, its effectiveness against Gram-negative bacterial infections has not been evaluated. This study aimed to assess whether Lcb. rhamnosus CRL1505 could beneficially regulate the respiratory innate immune response and improve resistance to hypermucoviscous KPC-2-producing Klebsiella pneumoniae of the sequence type 25 (ST25).
Article
Biochemistry & Molecular Biology
Mariano Elean, Fernanda Raya Tonetti, Kohtaro Fukuyama, Luciano Arellano-Arriagada, Fu Namai, Yoshihito Suda, Nadia Gobbato, Keita Nishiyama, Julio Villena, Haruki Kitazawa
Summary: In this study, nasally administered L. salivarius FFIG58 was found to modulate the innate immune response in the respiratory tract and provide long-term protection against Streptococcus pneumoniae infection. The strain enhanced the production of various immune factors and protected mice from secondary infection for up to 30 days.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mariano Elean, Leonardo Albarracin, Julio Villena, Haruki Kitazawa, Lucila Saavedra, Elvira M. Hebert
Summary: A comprehensive genomic analysis of L. delbrueckii strains was conducted, focusing on the proteolytic system and the key enzyme, cell envelope-associated proteinase (CEP). The study compared amino acid sequences and protein structures of CEPs, as well as analyzed syntenic and genomic variations among different strains. Investigation of CEP promoter region and regulatory elements provided insights into the control of proteolytic activity in lactobacilli. This study contributes to our understanding of proteolytic activity and potential applications of L. delbrueckii in the dairy industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Sudeb Saha, Kohtaro Fukuyama, Marina Debnath, Fu Namai, Keita Nishiyama, Haruki Kitazawa
Summary: This review focuses on the effect of probiotics on improving the quality of meat from small ruminants. The findings of the reviewed articles show inconsistent results due to the use of different probiotic strains, dosage rates, experiment duration, nutrition, breed, age, and animal health status. Future research should emphasize specific strains, optimal dose and days of administration, route, and mechanisms for the specific probiotic strains to host.
Article
Cell Biology
Hitoshi Watanabe, Wen Du, Jinsook Son, Lina Sui, Shun-ichiro Asahara, Irwin J. Kurland, Taiyi Kuo, Takumi Kitamoto, Yasutaka Miyachi, Rafael de Cabo, Domenico Accili
Summary: Sulfonylureas are effective and affordable antidiabetic drugs, but chronic use leads to secondary failure. Cytochrome b5 reductase 3 (Cyb5r3) down-regulation is identified as a mechanism of secondary SU failure and can be successfully reversed. Cyb5r3 activators can rescue secondary SU failure and potentially rehabilitate SU use in diabetes.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Review
Microbiology
Georgia Wunderlich, Michelle Bull, Tom Ross, Michael Rose, Belinda Chapman
Summary: The equine gastrointestinal tract houses a complex microbial consortium that breaks down plant material through fermentation. The primary digestion occurs in the large intestine, where bacteria, fungi, and archaea work together to break down complex plant polysaccharides. The unique interactions and powerful mechanisms employed by these microbes make the equine gut a valuable ecosystem to study fibrolytic functions.
Article
Medicine, Research & Experimental
Wen Du, Junqiang Wang, Taiyi Kuo, Liheng Wang, Wendy M. McKimpson, Jinsook Son, Hitoshi Watanabe, Takumi Kitamoto, Yunkyoung Lee, Remi J. Creusot, Lloyd E. Ratner, Kasi McCune, Ya-Wen Chen, Brendan H. Grubbs, Matthew E. Thornton, Jason Fan, Nishat Sultana, Bryan S. Diaz, Iyshwarya Balasubramanian, Nan Gao, Sandro Belvedere, Domenico Accili
Summary: The study demonstrates the potential of using the intestine as a source for cellular reprogramming to replace lost pancreatic beta cells in diabetes. By ablating FoxO1, the Paneth/goblet cell lineage can be converted into insulin-producing cells. Through a combination treatment targeting FOXO1, Notch, and TGF-beta, near normalization of glucose levels can be achieved in diabetic animals, accompanied by the generation of intestinal insulin-producing cells.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Masato Miyake, Mitsuaki Sobajima, Kiyoe Kurahashi, Akira Shigenaga, Masaya Denda, Akira Otaka, Tomohide Saio, Naoki Sakane, Hidetaka Kosako, Seiichi Oyadomari
Summary: A small molecule called KM04794 has been identified to alleviate protein aggregation and cell death caused by ER stress, and enhance insulin levels, thereby improving the function of pancreatic insulin-secreting cells.
CELL CHEMICAL BIOLOGY
(2022)