Article
Cell Biology
Janina Burk, Liza Wittenberg-Voges, Susanna Schubert, Carolin Horstmeier, Walter Brehm, Florian Geburek
Summary: The use of multipotent mesenchymal stromal cells (MSCs) shows promise in the treatment of early-phase tendon disease, with significant improvement in clinical inflammation, lameness, and ultrasonography scores over time. These findings provide a basis for larger controlled studies.
Article
Orthopedics
Riza Mert Cetik, Samiye Yabanoglu Ciftci, Betul Arica, Ipek Baysal, Saadet Ozen Akarca Dizakar, Fatma Kubra Erbay Elibol, Ayse Gencer, Teyfik Demir, Mehmet Ayvaz
Summary: Application of TGF-beta 3 enhances healing of Achilles tendon midsubstance injuries, and the use of nanoparticle form improves outcomes, specifically accelerating the remodeling process.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Review
Cell Biology
Vadim I. Chechekhin, Konstantin Yu. Kulebyakin, Romesh I. Kokaev, Pyotr A. Tyurin-Kuzmin
Summary: Adipose tissue relies on the regulation of multipotent mesenchymal stromal cells (MSCs), which serve as both precursors for new adipocytes and key paracrine regulators of adipose tissue homeostasis. The activity of MSCs is tightly controlled, regulating aspects such as proliferation, differentiation, and signal molecule production through modulation of hormonal sensitivity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nerea Cuesta-Gomez, Laura Medina-Ruiz, Gerard J. Graham, John D. M. Campbell
Summary: Mesenchymal stromal cells (MSC) are potential cellular therapeutics for psoriasis, a chronic inflammatory skin and joint disease. MSC adoptive cellular therapy can modulate the immune response and suppress the hyperactivation of effector T cells underlying the disease. In this study, we investigated the therapeutic potential of bone marrow and adipose tissue-derived MSCs in a psoriasis-like inflammation model. We found that both unlicensed and licensed MSCs accelerated the healing of psoriatic lesions, but unlicensed MSCs were more efficient in resolving skin inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Yun-Feng Zhou, Di Zhang, Wan-Ting Yan, Kai Lian, Zheng-Zheng Zhang
Summary: This article reviews the therapeutic potential of multipotent stromal cells (MSCs) in repairing meniscal injuries and discusses the avenues for utilizing these cells. The current progress on biomaterial implants is also reviewed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Endocrinology & Metabolism
Naomi Koehler, Leo Buhler, Bernhard Egger, Carmen Gonelle-Gispert
Summary: This review summarizes the immunomodulatory and anti-inflammatory properties of mesenchymal stromal cells (MSC) in the context of islet transplantation, highlighting the potential of co-culturing and co-transplanting islet cells with MSC to enhance islet proliferation and maturation, insulin secretion, and graft survival.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Ha Young Jang, Soo-Jin Kim, Kyu-Sang Park, Jeong Hun Kim
Summary: Degenerative changes of RPE triggered by TGF-beta 2 and oxidative stress play a critical role in AMD progression. The expression of antiaging protein alpha-klotho declines with age, increasing the risk of age-related diseases. Alpha-klotho protects against TGF-beta 2-induced RPE degeneration and EMT both in vitro and in vivo. It inhibits TGF-beta 2 signaling, EMT, oxidative stress, and senescence-associated signaling.
CELL DEATH & DISEASE
(2023)
Article
Cell & Tissue Engineering
Hengchen Liu, Mingzhao Zhang, Manyu Shi, Tingting Zhang, Wenjun Lu, Shulong Yang, Qingbo Cui, Zhaozhu Li
Summary: The study demonstrated that ADSC-Exos promoted the proliferation, migration, and tenogenic differentiation of TSCs, possibly through the activation of the SMAD signaling pathways. Additionally, ADSC-Exos inhibited early inflammatory reactions and promoted tendon healing in vivo.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell & Tissue Engineering
Hesham Soliman, Marine Theret, Wilder Scott, Lesley Hill, Tully Michael Underhill, Boris Hinz, Fabio M. Rossi
Summary: MSCs are essential for the development, maintenance, function, and regeneration of most tissues. They play a crucial role in responding to injury, promoting regeneration through secretion of trophic factors and ECM molecules, and contributing to fibrotic reparative processes. Through fate mapping and single cell omics, tissue-specific MSC identities and functions are being elucidated, providing unprecedented insights into the secret lives of tissue-resident MSCs.
Review
Neurosciences
Thong Teck Tan, Wei Seong Toh, Ruenn Chai Lai, Sai Kiang Lim
Summary: Mesenchymal Stromal Cell-secreted small extracellular vesicles (MSC-sEVs) have shown significant therapeutic efficacy in treating diseases, offering a safer and less complex alternative to living MSCs. However, realizing their full therapeutic potential will require strict scientific rigor and regulatory compliance.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elia Bari, Ilaria Roato, Giuseppe Perale, Filippo Rossi, Tullio Genova, Federico Mussano, Riccardo Ferracini, Marzio Sorlini, Maria Luisa Torre, Sara Perteghella
Summary: A new strategy was developed to improve the osteoinductivity of SmartBone (SB) by loading the SB scaffolds with freeze-dried formulation of mesenchymal stem cell (MSC)-secretome. The study showed that SBlyo significantly enhanced cell proliferation and tissue formation compared to SB, suggesting a more effective bone formation induction by SBlyo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Danielle M. Wiese, Catherine A. Wood, Barry N. Ford, Lorena R. Braid
Summary: This study compared the response of mesenchymal stromal cells (MSCs) from different donors and sources to cytokine activation. The results showed that the dynamic responses of licensed MSCs attenuated the heterogeneity between unstimulated populations and provided new insights into standardized assays.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Zhenbo Tu, Antoine E. Karnoub
Summary: Mesenchymal stem/stromal cells (MSCs) are a heterogeneous population of fibroblastic progenitor cells that reside in multiple tissues around the body and have the ability to differentiate into various connective tissue cells. They play critical regulatory roles in breast tumor development and progression, participating in multiple aspects of the process.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Immunology
Matthew D. Patrick, Ramkumar T. Annamalai
Summary: This study enhances the immunomodulatory potential of mesenchymal stromal cells (MSC) by priming them with IFN gamma and TNF alpha, and develops gelatin-based microgels. These microgels possess adsorption capacity and sustained release effect, showing great potential in immunomodulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elia Bari, Fulvio Tartara, Fabio Cofano, Giuseppe di Perna, Diego Garbossa, Sara Perteghella, Marzio Sorlini, Delia Mandracchia, Lorella Giovannelli, Paolo Gaetani, Maria Luisa Torre, Lorena Segale
Summary: The study found that the mesenchymal stem cell-secretome Lyosecretome has a significant improvement effect on the osteoinductive and osteoconductive properties of titanium cages, leading to increased mineralized matrix when used during culturing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Mohamad Al Naem, Lutz-Ferdinand Litzke, Klaus Failing, Janina Burk, Michael Roecken
Summary: This study compared the load distribution pattern in healthy horses and horses with laminitis, finding differences in loading patterns in the toe region. Supportive therapy in laminitis should focus on supporting the caudal and middle hoof areas.
EQUINE VETERINARY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
A. Hagen, H. Lehmann, S. Aurich, N. Bauer, M. Melzer, J. Moellerberndt, V. Patane, C. L. Schnabel, J. Burk
Summary: This study aimed to establish a scalable protocol for equine platelet lysate (ePL) production and to test its effectiveness in equine MSC culture. Platelet concentrates prepared using the buffy coat method showed increased platelet and reduced white blood cell content, and were used successfully as a supplement in MSC culture. Further research into the functionality of equine MSC with ePL as a culture medium should be conducted.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Carla U. Doll, Sabine Niebert, Janina Burk
Summary: Tendon lesions are common in both humans and horses, with the healing process often leading to fibrosis and chronic disease. Local mesenchymal stromal cell injection is a accepted therapeutic strategy for acute lesions in horses, but data on its efficacy in chronic tendon disease are limited. However, MSCs have been shown to have a positive effect on fibrotic tissue. This study aimed to investigate the interaction between MSCs and healthy or chronically diseased tendon matrix in horses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Janina Burk, Anna Sassmann, Cornelia Kasper, Ariane Nimptsch, Susanna Schubert
Summary: Matrix remodeling by MSC in ECM disease could be influenced by the ECM environment. MSC adapt to the ECM conditions, including pathological adaptations, which may affect therapeutic efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Veterinary Sciences
Nadia Ayurini Anantama, Charis Du Cheyne, Ann Martens, Susanne Pauline Roth, Janina Burk, Ward De Spiegelaere, Jule Kristin Michler
Summary: Exuberant granulation tissue (EGT) is commonly observed in horses during second intention wound healing. The mechanisms leading to EGT are still unclear, and effective strategies to prevent and treat EGT are lacking. This study conducted a systematic search and scoping review to consolidate the literature on EGT and identify knowledge gaps and research opportunities. The results highlight the need for evidence-based recommendations on EGT treatment and the importance of incorporating basic research knowledge into clinical trials.
VETERINARY JOURNAL
(2022)
Article
Agriculture, Dairy & Animal Science
Alina Hagen, Heidrun Holland, Vivian-Pascal Brandt, Carla U. Doll, Thomas C. Haeussler, Michaela Melzer, Julia Moellerberndt, Hendrik Lehmann, Janina Burk
Summary: This study investigates the suitability of canine platelet lysate for feeding stem cell cultures and compares it with equine platelet lysate. The results showed that canine platelet lysate did not support canine stem cell culture as effectively as equine platelet lysate. Further research is needed to improve the cultivation of canine stem cells.
Article
Cell Biology
Janina Burk, Michaela Melzer, Alina Hagen, Katrin Susanne Lips, Katja Trinkaus, Ariane Nimptsch, Jenny Leopold
Summary: This study investigated the applicability of lipid phenotyping for MSC characterization and discrimination, finding differences in phospholipid species diversity between MSC from different species, and identified potentially suitable lipid markers across culture conditions. Phospholipid profiling could be a promising approach for MSC characterization, allowing for some heterogeneity within MSC while still achieving clear discrimination from fibroblasts.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Susanne Pauline Roth, Janina Burk, Walter Brehm, Antonia Troillet
Summary: Mesenchymal stromal cells (MSC) are a promising treatment option for tendon and joint diseases. Their therapeutic efficacy is influenced by the microenvironment and cellular components, with inflammation and macrophage modulation playing important roles. Target cells include tenocytes and immune cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Veterinary Sciences
Alina Hagen, Sabine Niebert, Vivian-Pascal Brandt, Heidrun Holland, Michaela Melzer, Axel Wehrend, Janina Burk
Summary: This study analyzed the functional properties of equine adipose-derived MSC cultured with equine platelet lysate (ePL), and found that ePL not only supported MSC's proliferation and basic characteristics, but also enhanced their cell fitness and pro-angiogenic potency.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Julia Moellerberndt, Alina Hagen, Sabine Niebert, Kathrin Buettner, Janina Burk
Summary: In equine medicine, the use of regenerative therapeutics, particularly platelet lysate (PL), is being explored as a potential treatment option. This study aims to analyze the cytokine concentrations in different blood components and investigate their correlation with abnormal blood chemistry findings. The results show that cytokine concentrations in serum, plasma, platelet concentrate, and PL are similar and significantly correlated. However, there is a high inter-individual variability in cytokine concentrations, with some horses having very high concentrations while others have none. The study suggests that the variability in cytokine concentrations may have implications for the therapeutic potential of PL, and blood chemistry analysis can be used to predict cytokine levels.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Carla Ulrike Doll, Melanie Bohner, Dagmar Berner, Kathrin Buettner, Carolin Horstmeier, Karsten Winter, Janina Burk
Summary: This study aimed to improve the reliability, comparability, and time efficiency of quantitative MRI image analysis for monitoring equine tendon lesions. The results suggest that the signal intensity of tendon lesions can be standardized using a quotient of lesion and background or cortical bone signal intensities, and circular regions of interest can be used for lesion signal intensity measurement. The study also showed that automated measurement methods have good feasibility in terms of time efficiency and accuracy.
VETERINARY RECORD OPEN
(2023)
Meeting Abstract
Cell & Tissue Engineering
Michaela Melzer, Susanna Schubert, Simon Franz Mueller, Joachim Geyer, Alina Hagen, Sabine Niebert, Janina Burk
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Carla Doll, Kerstin von Pueckler, Julia Offhaus, Janina Burk
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Alina Hagen, Hendrik Lehmann, Vivian-Pascal Brandt, Heidrun Holland, Michaela Melzer, Julia Moellerberndt, Christiane Liliane Schnabel, Janina Burk
TISSUE ENGINEERING PART A
(2022)
Article
Veterinary Sciences
Carla Ulrike Doll, Kerstin von Pueckler, Julia Offhaus, Dagmar Berner, Janina Burk
Summary: This study investigated the application of MRI in chronic tendon lesions in equine medicine, and compared the impact of different sequences and tendon positioning on lesion detection. The results showed that the T1w FLASH sequence with vertical tendon positioning in high-field MRI was the most effective for diagnosing tendon lesions, while the T1w SE sequence with horizontal tendon positioning had poor performance.
VETERINARY SCIENCES
(2022)