Article
Cell Biology
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Wei-Chun Tang, Chieh-Han Lu, Stephanie E. Mohr, Robert V. Farese, Tobias C. Walther
Summary: The study identifies two protein targeting pathways from the endoplasmic reticulum to lipid droplets and defines the key factors and mechanisms involved. These pathways play essential roles in the organization and function of eukaryotic cells.
NATURE CELL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yuping Zhao, Hyeong Seok Kim, Xiang Zou, Ling Huang, Xing Liang, Zihong Li, Jong Seung Kim, Weiying Lin
Summary: This study proposed a new strategy to investigate the interaction regulatory mechanisms between endoplasmic reticulum and autophagosomes by developing dual fluorescence lifetime probes localized to these two organelles. The research revealed that motile autophagosomes at the tips of endoplasmic reticulum drive the growth and sliding of ER tubules, ER reticulate tubules form a three-way junction centered on autophagosomes, and ER autophagy serves as a cell damage index during drug-induced apoptosis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Multidisciplinary Sciences
Amelie Bonaud, Laetitia Gargowitsch, Simon M. Gilbert, Elanchezhian Rajan, Pablo Canales-Herrerias, Daniel Stockholm, Nabila F. Rahman, Mark O. Collins, Hakan Taskiran, Danika L. Hill, Andres Alloatti, Nagham Alouche, Stephanie Balor, Vanessa Soldan, Daniel Gillet, Julien Barbier, Francoise Bachelerie, Kenneth G. C. Smith, Julia Jellusova, Pierre Bruhns, Sebastian Amigorena, Karl Balabanian, Michelle A. Linterman, Andrew A. Peden, Marion Espeli
Summary: We identified SNARE Sec22b as a critical regulator of plasma cell maintenance and function. In the absence of Sec22b, plasma cells were hardly detectable and serum antibody titers were dramatically reduced, leading to a failure in mounting a protective immune response. Mechanistically, Sec22b contributes to efficient antibody secretion and is involved in regulating plasma cell transcriptional identity, as well as the morphology of the endoplasmic reticulum and mitochondria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Jaideep Mathur
Summary: Plants possess the ability to quickly respond to environmental changes at the cellular level. This is achieved through the formation of various organelle extensions, which continuously change shape and interact with the endoplasmic reticulum and cytoskeletal elements to increase organelle outreach into the surrounding cytoplasm. These extensions play a common role in maintaining homeostasis in plant cells.
Article
Biochemistry & Molecular Biology
Jingze Zang, Sandra Klemm, Charlotte Pain, Patrick Duckney, Zhiru Bao, Gina Stamm, Verena Kriechbaumer, Katharina Buerstenbinder, Patrick J. Hussey, Pengwei Wang
Summary: This study identified a crucial cytoskeletal-associated complex that is essential for maintaining and organizing cytoskeletal structure and ER morphology in plant cells.
Article
Plant Sciences
Mingjing Wang, Hailong Zhang, Xiaonan Zhao, Jingwen Zhou, Guochen Qin, Yuqi Liu, Xiaoyue Kou, Zhenjie Zhao, Tao Wu, Jian-Kang Zhu, Xianzhong Feng, Lixin Li
Summary: The gene AtSYP81 in Arabidopsis thaliana plays a critical role in root growth and development by regulating peroxisome- and peroxidase-mediated ROS homeostasis. Knockdown of AtSYP81 resulted in reduced root meristem activity and disruption of root stem cell niche identity. Transcriptome analysis revealed changed expression of class III peroxidases, adding evidence of the regulatory role of AtSYP81 in ROS signaling.
Review
Plant Sciences
Kazue Kanehara, Yueh Cho, Chao-Yuan Yu
Summary: This review discusses the plant unfolded protein response and compares it with the respective systems in other model organisms from the perspective of membrane phospholipids.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Plant Sciences
Jiahui Bian, Xiao Su, Xiaoyan Yuan, Yuan Zhang, Jinxing Lin, Xiaojuan Li
Summary: This review focuses on the structural organization, dynamics, and physiological functions of membrane contact sites between the endoplasmic reticulum and various organelles, particularly in plants. Membrane contact sites play a vital role in development and response to stress by facilitating highly dynamic and organized interactions among organelles. The endoplasmic reticulum acts as an architectural scaffold to maintain the spatial distribution of other membrane-bound organelles. Future research directions related to membrane contact sites are also discussed.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Francesca Resentini, Matteo Grenzi, Daniele Ancora, Mara Cademartori, Laura Luoni, Marianna Franco, Andrea Bassi, Maria Cristina Bonza, Alex Costa
Summary: The study utilizes genetically encoded Ca2+ indicators to reveal details of Ca2+ dynamics in plant cells, including the processing time of Ca2+ from the cytosol to the ER and the time to observe cytosolic Ca2+ increase with ER inhibition. Live imaging of mature plants also shows a wounding-induced long-distance ER Ca2+ wave, enhancing the understanding of intracellular Ca2+ dynamics and signaling.
Article
Cell Biology
Ben Cardoen, Kurt R. Vandevoorde, Guang Gao, Milene Ortiz-Silva, Parsa Alan, William Liu, Ellie Tiliakou, A. Wayne Vogl, Ghassan Hamarneh, Ivan R. Nabi
Summary: This study applies the subpixel resolution MCS-DETECT algorithm to analyze 3D super-resolution image volumes, revealing the dual control mechanism of tubular riboMERCs. The formation of riboMERCs relies on RRBP1 and is modulated by Gp78 E3 ubiquitin ligase activity.
JOURNAL OF CELL BIOLOGY
(2023)
Letter
Plant Sciences
Philippe Fuchs, Elisenda Feixes-Prats, Paulo Arruda, Elias Feitosa-Araujo, Alisdair R. Fernie, Christopher Grefen, Sophie Lichtenauer, Nicole Linka, Ivan de Godoy Maia, Andreas J. Meyer, Soeren Schilasky, Lee J. Sweetlove, Stefanie Wege, Andreas P. M. Weber, A. Harvey Millar, Olivier Keech, Igor Florez-Sarasa, Pedro Barreto, Markus Schwarzlaender
Summary: UCP2 is localized to the Golgi, unlike its close homolog UCP1, which is abundant in the mitochondria.
Article
Cell Biology
Nari Kim, Tae-Hyeon Kim, Chaelim Kim, Jee-Eun Lee, Myeong-Gyun Kang, Sanghee Shin, Minkyo Jung, Jong-Seo Kim, Ji Young Mun, Hyun-Woo Rhee, Seung-Yeol Park, Yongdae Shin, Joo-Yeon Yoo
Summary: Under cellular stresses, COPII proteins are sequestered by the biomolecular condensate of SCOTIN/SHISA-5, inhibiting COPII vesicle formation and ER-to-Golgi transport.
DEVELOPMENTAL CELL
(2023)
Article
Cell Biology
Paul Heo, Jacob A. Culver, Jennifer Miao, Frederic Pincet, Malaiyalam Mariappan
Summary: The Get1/2 complex forms an aqueous channel that can insert the transmembrane domain of TA proteins into the membrane and release them into the endoplasmic reticulum. The channel activity of Get1/2 is essential for protein release and translocation.
Article
Plant Sciences
Enrico Cortese, Roberto Moscatiello, Francesca Pettiti, Luca Carraretto, Barbara Baldan, Lorenzo Frigerio, Ute C. Vothknecht, Ildiko Szabo, Diego De Stefani, Marisa Brini, Lorella Navazio
Summary: This study successfully achieved precise measurements of dynamic changes in free Ca2+ concentration in the lumen of the plant endoplasmic reticulum using an aequorin chimera with reduced Ca2+ affinity, revealing rapid and transient increases of the ER luminal Ca2+ concentration in response to different environmental stresses. Comparative analysis also indicates a complex interplay between ER and chloroplast Ca2+ dynamics in shaping cytosolic Ca2+ signals during signal transduction events.
Article
Multidisciplinary Sciences
Laila Giordano, Valerie Allasia, Alexandra Cremades, Sophie Hok, Franck Panabieres, Beatrice Bailly-Maitre, Harald Keller
Summary: This study revealed a previously uncharacterized role of a plant receptor domain in the regulation of ER stress during infection.
