Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Review
Cell Biology
Xue Jiang, Yihuan Chen, Xiaofeng Liu, Lingqun Ye, Miao Yu, Zhenya Shen, Wei Lei, Shijun Hu
Summary: Researchers have discovered the contribution of genetic defects to the pathogenesis of primary cardiomyopathy and tried to explain the pathogenesis of these diseases by establishing various disease models. The advent of human induced pluripotent stem cells (hiPSCs) provides an unprecedented opportunity to further investigate the pathogenic mechanisms of inherited cardiomyopathies in vitro using patient-specific hiPSC-derived cardiomyocytes. The development of defects in genetically modified animal models differs notably from human diseases at the molecular level.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lauren Michelle Walker, Nicole R. L. Sparks, Veronica Puig-Sanvicens, Beatriz Rodrigues, Nicole zur Nieden
Summary: This study updated the classic mouse EST with endpoints derived from human induced pluripotent stem cells (hiPSCs) and human fibroblasts, showing improved prediction of human risks; the hiPSC-EST was able to detect inhibition of differentiation at lower concentrations and adverse developmental outcomes caused by novel environmental toxicants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Taiki Satoh, Marcelo A. S. Toledo, Janik Boehnke, Kathrin Olschok, Niclas Flosdorf, Katrin Goetz, Caroline Kuestermann, Stephanie Sontag, Kristin Sere, Steffen Koschmieder, Tim H. Bruemmendorf, Nicolas Chatain, Yoh-ichi Tagawa, Martin Zenke
Summary: Dendritic cells (DC) are professional antigen-presenting cells that can be divided into different subsets, with the DC3 subset having proinflammatory properties. Human iPS cell-derived DC3 exhibit similar characteristics to blood DC3 in vitro. The JAK2 V617F mutation enhances DC3 production and biases towards erythrocytes and megakaryocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Alexandra Neaverson, Malin H. L. Andersson, Osama A. Arshad, Luke Foulser, Mary Goodwin-Trotman, Adam Hunter, Ben Newman, Minal Patel, Charlotte Roth, Tristan Thwaites, Helena Kilpinen, Matthew E. Hurles, Andrew Day, Sebastian S. Gerety
Summary: Efficient and effective methods for converting human induced pluripotent stem cells into differentiated derivatives are critical for performing robust, large-scale studies of development and disease modelling, and for providing a source of cells for regenerative medicine. Here, we describe a 14-day neural differentiation protocol which allows for the scalable, simultaneous differentiation of multiple iPSC lines into cortical neural stem cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Environmental
Hyejoong Jeong, Wijin Kim, Daheui Choi, Jiwoong Heo, Uiyoung Han, Se Yong Jung, Hee Ho Park, Sung-Tae Hong, Ju Hyun Park, Jinkee Hong
Summary: The study found that NPs can be internalized into cells within a short period of time, leading to significant growth inhibition of hiPSCs. However, the accumulated NPs did not have adverse effects on cell differentiation.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Chemistry, Medicinal
Megan A. Yamoah, Phung N. Thai, Xiao-Dong Zhang
Summary: Nanotechnology offers a promising approach for transgene delivery into human induced pluripotent stem cells (hiPSCs) with advantages including high efficiency, low cytotoxicity, and potential for successful applications in regenerative and precision medicine.
Article
Biochemistry & Molecular Biology
Bing-Chun Liu, Fang-Yuan Liu, Xin-Yue Gao, Yang-Lin Chen, Qiao-Qiao Meng, Yong-Li Song, Xi-He Li, Si-Qin Bao
Summary: The study reveals that Wnt signaling plays a crucial role in driving human pluripotent stem cells towards neural stem cells differentiation and maintaining their self-renewal. CLNSCs exhibit spinal cord properties and high proliferative capacity, providing a new cell model for investigating neurogenesis mechanisms and potential applications in regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Engineering, Multidisciplinary
Ikki Horiguchi, Masahiro Kino-oka
Summary: Induced pluripotent stem cells (iPSCs) are considered promising cell sources for regenerative medicine and drug screening, but effective mass production systems are still lacking. Various factors such as nutrient supply, waste removal, and oxygen availability need to be controlled for stable iPSC production. Multidisciplinary investigations are essential for understanding the factors influencing cell growth and quality in order to achieve an optimal and stable iPSC mass production system.
Review
Cell Biology
Juan Huang, Qi Feng, Li Wang, Bingying Zhou
Summary: Cardiac diseases are the leading cause of global mortality, but limited progress has been made in developing therapeutic options. The use of animal models has limitations due to species-specific differences, leading to inaccurate drug testing results. Human pluripotent stem cell (hPSC) technology offers promising alternatives for heart disease modeling, cell therapy, and drug discovery, with potential for revolutionizing these fields with further optimization required.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Wendy Keung, Yiu-Fai Cheung
Summary: Chemotherapies for cancer treatment often lead to severe side effects, with cardiotoxicity being a notable concern. Identifying risk factors and understanding the underlying mechanisms of cardiotoxicity are essential for improving clinical outcomes. Human induced pluripotent stem cell technology offers a promising platform for validating risk factors and advancing personalized medicine.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Endocrinology & Metabolism
Diego Balboa, Diepiriye G. Iworima, Timothy J. Kieffer
Summary: The review discusses the recent progress in generating and characterizing functional stem cell-derived beta cells, as well as the possibilities and challenges in diabetes disease modeling using stem cells.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Cell Biology
Mario G. Pavez-Giani, Lukas Cyganek
Summary: This review summarizes recent advances in iPSC-based disease modeling of mitochondrial cardiomyopathies and explores the patho-mechanistic insights as well as new therapeutic approaches. Researchers have used iPSC technology to recapitulate major characteristics of mitochondrial cardiomyopathies, providing a powerful platform for drug development and testing of new therapies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Jernej Murn, Kathi Zarnack, Yawei J. Yang, Omer Durak, Elisabeth A. Murphy, Sihem Cheloufi, Dilenny M. Gonzalez, Marianna Teplova, Tomaz Curk, Johannes Zuber, Dinshaw J. Patel, Jernej Ule, Nicholas M. Luscombe, Li-Huei Tsai, Christopher A. Walsh, Yang Shi
GENES & DEVELOPMENT
(2015)
Article
Neurosciences
Andrii Rudenko, Jinsoo Seo, Ji Hu, Susan C. Su, Froylan Calderon de Anda, Omer Durak, Maria Ericsson, Marie Carlen, Li-Huei Tsai
JOURNAL OF NEUROSCIENCE
(2015)
Article
Cell Biology
Froylan Calderon de Anda, Ram Madabhushi, Damien Rei, Jia Meng, Johannes Graeff, Omer Durak, Konstantinos Meletis, Melanie Richter, Birgit Schwanke, Alison Mungenast, Li-Huei Tsai
Article
Neurosciences
Omer Durak, Fan Gao, Yea Jin Kaeser-Woo, Richard Rueda, Anthony J. Martorell, Alexi Nott, Carol Y. Liu, L. Ashley Watson, Li-Huei Tsai
NATURE NEUROSCIENCE
(2016)
Article
Multidisciplinary Sciences
Anusha Narayan, Vivek Venkatachalam, Omer Durak, Douglas K. Reilly, Neelanjan Bose, Frank C. Schroeder, Aravinthan D. T. Samuel, Jagan Srinivasan, Paul W. Sternberg
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Biology
Jagan Srinivasan, Omer Durak, Paul W. Sternberg
Article
Cell Biology
Joshua J. Buchman, Omer Durak, Li-Huei Tsai
GENES & DEVELOPMENT
(2011)
Article
Neurosciences
Froylan Calderon de Anda, Ana Lucia Rosario, Omer Durak, Tracy Tran, Johannes Graeff, Konstantinos Meletis, Damien Rei, Takahiro Soda, Ram Madabhushi, David D. Ginty, Alex L. Kolodkin, Li-Huei Tsai
NATURE NEUROSCIENCE
(2012)
Article
Multidisciplinary Sciences
Fatma Kaplan, Jagan Srinivasan, Parag Mahanti, Ramadan Ajredini, Omer Durak, Rathika Nimalendran, Paul W. Sternberg, Peter E. A. Teal, Frank C. Schroeder, Arthur S. Edison, Hans T. Alborn
Article
Multidisciplinary Sciences
Ji-Song Guan, Susan C. Su, Jun Gao, Nadine Joseph, Zhigang Xie, Ying Zhou, Omer Durak, Lei Zhang, J. Julius Zhu, Karl R. Clauser, Steven A. Carr, Li-Huei Tsai
