Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Cell & Tissue Engineering
Hye Ji Moon, Na Yeon Lee, Eun Kyoung Do, Seo Yul Lee, Gyu Tae Park, Jae Kyong Lim, Jeong Kon Seo, Jae Ho Kim
Summary: In this study, Kap1 was identified as a novel Lin28A-binding protein, which regulates the stability of Lin28A through modulating the Trim71-mediated ubiquitination and degradation of Lin28A, thus playing a crucial role in the regulation of ESC self-renewal and pluripotency.
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Jose Manuel Inacio, Joao von Gilsa Lopes, Ana Mafalda Silva, Fernando Cristo, Sara Marques, Matthias E. Futschik, Jose Antonio Belo
Summary: Deciphering the clues of a regenerative mechanism for the mammalian adult heart, particularly focusing on the role of DAND5, could potentially lead to the development of more effective treatment options for heart failure. Further research on the transcriptional profiling sequencing and quantitative RT-PCR assays of DAND5 may provide insights into the generation and expansion of pluripotent stem cell-derived cardiomyocytes systems for clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Chenze Xu, Ali Mohsin, Yanxia Luo, Lili Xie, Yan Peng, Qizheng Wang, Waqas Ahmed, Haifeng Hang, Yingping Zhuang, Meijin Guo
Summary: In this study, non-genetically modified Sertoli-like cells were successfully induced from mouse ESCs using defined protein factors, showing similar characteristics and functionality to natural Sertoli cells. However, the efficiency of the non-GM method was still lower compared to lentiviral transduction. This work lays a foundation for the future production of non-GM Sertoli-like cells for clinical applications and fundamental theory research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Hematology
Chrysa Kapeni, Leslie Nitsche, Alastair M. Kilpatrick, Nicola K. Wilson, Kankan Xia, Bahar Mirshekar-Syahkal, Vashe Chandrakanthan, Camille Malouf, John E. Pimanda, Berthold Gottgens, Kristina Kirschner, Simon R. Tomlinson, Katrin Ottersbach
Summary: This study shows that the cell cycle inhibitor p57Kip2/Cdkn1c limits the number of hematopoietic stem cells by restricting the size of the sympathetic nervous system and the amount of catecholamines secreted by these cells. This regulation occurs at the sympathetic nervous system progenitor level and has a significant impact on early fetal liver colonization.
Article
Pharmacology & Pharmacy
Ying Xia, Bin Jiang, Zhongkun Teng, Tiantian Liu, Jianming Wang, Stanley Aniagu, Guoxing Zhang, Tao Chen, Yan Jiang
Summary: This study aimed to investigate the toxic effects and mechanisms of trichloroethylene (TCE) on human embryonic stem cells (hESCs) proliferation. The results showed that TCE enhanced hESCs proliferation by upregulating the expression of Cx43 and cyclin-dependent kinases. In addition, TCE increased Akt phosphorylation, which further stimulated the overexpression of Cx43 and cell proliferation.
Review
Cell & Tissue Engineering
Gamze Ayaz, Hualong Yan, Navdeep Malik, Jing Huang
Summary: The TP53 gene encodes the p53 protein, a tumor suppressor that plays a crucial role in maintaining genome stability. Recent studies have found that p53 is a critical regulator of pluripotency, self-renewal, differentiation, proliferation, and genome stability in embryonic stem cells.
Article
Cell Biology
Xiaofen Liu, Wenjuan Zhang, Changbin Jing, Lei Gao, Cong Fu, Chunguang Ren, Yimei Hao, Mengye Cao, Ke Ma, Weijun Pan, Dantong Li
Summary: This study identified a novel zebrafish mutant line with a mutation in the Gemin5 gene, leading to specific deficiency in definitive hematopoiesis, which was attributed to impaired proliferation of HSPCs. The results suggest that Gemin5 plays an essential role in regulating HSPCs proliferation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ryuki Shimada, Hiroko Koike, Takamasa Hirano, Yuzuru Kato, Yumiko Saga
Summary: During murine germ cell development, NANOS2 plays a key role in suppressing the cell cycle and initiating sexually dimorphic differentiation. Using single-cell RNA sequencing, it was revealed that NANOS2 inhibits mTORC1 activity to suppress the cell cycle, potentially through target proteins like Ptma.
Article
Multidisciplinary Sciences
Yanzhao Wei, Xiaohan Zheng, Ting Huang, Yuanji Zhong, Shengtong Sun, Xufang Wei, Qibing Liu, Tan Wang, Zhenqiang Zhao
Summary: This study found for the first time that MIF is highly expressed in human embryonic stem cells and exerts its effects through interactions with its receptors.
Article
Multidisciplinary Sciences
Aditya Kshirsagar, Svetlana Maslov Doroshev, Anna Gorelik, Tsviya Olender, Tamar Sapir, Daisuke Tsuboi, Irit Rosenhek-Goldian, Sergey Malitsky, Maxim Itkin, Amir Argoetti, Yael Mandel-Gutfreund, Sidney R. Cohen, Jacob H. Hanna, Igor Ulitsky, Kozo Kaibuchi, Orly Reiner
Summary: LIS1 is a crucial gene associated with neurodevelopmental diseases and regulates the activity of cytoplasmic dynein. This study reveals that LIS1 is essential for the viability of mouse embryonic stem cells (mESCs) and also influences gene expression and interacts with RNA and RNA-binding proteins.
NATURE COMMUNICATIONS
(2023)
Article
Neurosciences
Olga Gordeeva, Vitaliy Safandeev
Summary: In this study, the regulatory effects of serotonin precursor 5-HTP on cell proliferation and cell death in mouse embryonic stem cells and embryonic fibroblasts were investigated. The results showed that 5-HTP treatment inhibited cell proliferation, induced cell cycle arrest, and increased apoptotic and necrotic cell death. Furthermore, 5-HTP treatment upregulated the gene expression of enzymes involved in 5-HT synthesis and metabolism, leading to toxic effects through enhancing protein modifications mediated by 5-HT.
Article
Engineering, Biomedical
Tingting Gao, Xiyuan Zhao, Jie Hao, Yao Tian, Huike Ma, Wenjing Liu, Bin An, Faguo Sun, Shasha Liu, Baojie Guo, Shuaishuai Niu, Zhongwen Li, Chenxin Wang, Yukai Wang, Guihai Feng, Liu Wang, Wei Li, Jun Wu, Meijin Guo, Qi Zhou, Qi Gu
Summary: In this study, a new microcarrier with hyaluronic acid (HA) as the core material was developed for scalable serum-free suspension culture of hESC-derived MSCs. The optimal microcarriers, with a coating collagen concentration of 100 μg/mL or concave-structured surface (cHAMCs), allowed for the expansion of IMRCs within six days with a high yield. The harvested cells showed high viability, immunomodulatory and regenerative therapeutic promise, as well as increased secretion of extracellular matrix (ECM).
MATERIALS TODAY BIO
(2023)
Article
Developmental Biology
Yu Shi, Nitya Kopparapu, Lauren Ohler, Daniel J. Dickinson
Summary: Fluorescent protein tagging is a crucial method for studying protein behavior in living cells. However, overexpression of tagged proteins can disrupt cell behavior. This study presents an improved method for inserting fluorescent proteins directly into native genes in mammalian cells using the CRISPaint system. The protocol is efficient and enables live imaging in less than two weeks post-transfection, increasing the versatility of fluorescent protein knock-in.
