Article
Biochemical Research Methods
Jialin Chen, Fengli Wang, Chengzhi He, Shi-Zhong Luo
Summary: Syndecans (SDCs) are a family of integral membrane proteins that play important roles in cell-cell interactions. The dimerization/oligomerization generated by transmembrane domains (TMDs) crucially regulates the functional behaviors of SDCs, with different dimerizing motifs affecting the stability of SDC dimers. Among the SDC dimers studied, SDC2 forms the most stable homodimer due to the presence of specific dimerizing motifs.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2021)
Article
Cell Biology
Yening Xiao, Jiamei Ma, Chunliu Guo, Danni Liu, Jing Pan, Xiaoxi Huang
Summary: This study demonstrates that silencing of the CCNB2 gene can inhibit tumor formation and proliferation in nude mice. CCNB2 may accelerate the proliferation and metastasis of hepatocellular carcinoma cells by increasing JAG1 expression.
Article
Veterinary Sciences
Lynn Pezzanite, Lyndah Chow, Gregg Griffenhagen, Steven Dow, Laurie Goodrich
Summary: This study compared the immunomodulatory and antibacterial properties of equine mesenchymal stromal cells (MSCs) cultured in different serum sources. Results showed that MSCs cultured in FBS exhibited improved functionality compared to equine serum, while there were no significant differences between cells cultured in autologous vs. allogeneic equine serum.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Public, Environmental & Occupational Health
Lisa Wanders, Esmee A. Bakker, Hein P. J. van Hout, Thijs M. H. Eijsvogels, Maria T. E. Hopman, Leonie N. C. Visser, Hans Wouters, Dick H. J. Thijssen
Summary: The study found a strong positive association between total sedentary behavior and cognitive function in a heterogenous population. This relationship was not consistent across different domains, with work- and computer-related sedentary behavior showing a particularly strong positive association with cognitive function. This highlights the importance of assessing various sedentary domains in understanding the relationship between sedentary time and cognitive function.
PREVENTIVE MEDICINE
(2021)
Article
Cell Biology
Chang Cai, Shuo Yao, Yanmei Zou, Hui Lu, Xiuqiong Chen, Yali Wang, Kun Zheng, Feng Zhu, Yihua Wang, Hua Xiong, Junfei Zhu
Summary: KRAS(G12C) mutation is associated with upregulation of T-LAK cell-originated protein kinase (TOPK), which promotes the malignant phenotype of A549 cells with KRAS(G12C) mutation. TOPK level is regulated by MAPK/ERK signalling and the transcription factor Elk1. It also activates the NF-kappa B signalling pathway by facilitating the phosphorylation of TAK1. Targeting the KRAS-TOPK axis synergizes with existing chemotherapeutics in treating NSCLC.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Simone R. Potje, Ayman Isbatan, Rita C. Tostes, Lusiane M. Bendhack, Randal O. Dull, Joao L. Carvalho-de-Souza, Andreia Z. Chignalia
Summary: The study demonstrated that glypican 1 plays a crucial role in the development of noradrenergic hypertension by acting through IP3R and calcium-dependent signaling pathways. Knockout mice lacking glypican 1 were uniquely protected from a norepinephrine hypertensive challenge, indicating its potential as a target for new therapies for resistant hypertension or conditions with increased norepinephrine levels.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Medical Laboratory Technology
Fang Liu, Linyan Cao, Yufang Zhang, Xinyi Xia, Yanhua Ji
Summary: The study revealed that low expression of LIFR-AS1 in SOC is associated with poor prognosis and tumor characteristics. Overexpression of LIFR-AS1 inhibited the malignant behaviors of SOC cells while promoting apoptosis and epithelial marker expression. Silencing LIFR-AS1 had opposite effects, indicating its potential as a therapeutic target.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Gastroenterology & Hepatology
Lea Duwe, Patricia Munoz-Garrido, Monika Lewinska, Juan Lafuente-Barquero, Letizia Satriano, Dan Hogdall, Andrzej Taranta, Boye S. Nielsen, Awaisa Ghazal, Matthias S. Matter, Jesus M. Banales, Blanca I. Aldana, Yu-Tang Gao, Jens U. Marquardt, Lewis R. Roberts, Rui C. Oliveira, Jill Koshiol, Colm J. O'Rourke, Jesper B. Andersen
Summary: This study characterized the dysregulation of microRNAs (miRNAs) in cholangiocarcinoma (CCA) and their impact on transcriptome homeostasis and cell behavior. miRNA sequencing and screening identified miR-27a-3p as an oncogenic vulnerability in CCA, which promoted tumor growth by downregulating FOXO signaling. The findings highlight the remodeling of miRNomes in CCA and suggest miR-27a-3p as a potential therapeutic target for CCA.
JOURNAL OF HEPATOLOGY
(2023)
Article
Oncology
Ana Pavlic, Emanuela Bostjancic, Rajko Kavalar, Bojan Ilijevec, Serena Bonin, Fabrizio Zanconati, Nina Zidar
Summary: This study investigates the involvement of epithelial-mesenchymal transition (EMT) in tumor budding (TB) and poorly differentiated clusters (PDCs) in colorectal cancer (CRC). The results suggest that both TB and PDCs are associated with partial EMT, but they exhibit discrete differences in morphology and expression of EMT-related markers.
JOURNAL OF PATHOLOGY
(2022)
Article
Endocrinology & Metabolism
Bo Xiao, Mingwei Li, Mingxuan Cui, Chengliang Yin, Bo Zhang
Summary: The purpose of this study was to establish a comprehensive tumour microenvironment (TME)-relevant prognostic gene and target miRNA network for breast cancer patients. Based on a large-scale screening of TME-relevant prognostic genes for breast cancer patients, a prognostic model was established. The relationships between the potential TME prognostic genes and the prognosis of breast cancer patients were explored, and the functions of each primary TME member were investigated in the breast cancer cell line.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Alessandro Zucchi, Francesco Claps, Antonio Luigi Pastore, Alessandro Perotti, Andrea Biagini, Luana Sallicandro, Rosaria Gentile, Concetta Caglioti, Federico Palazzetti, Bernard Fioretti
Summary: Bladder cancer is a common urinary system tumor, especially in males. Surgery and intravesical instillations can eliminate it, but recurrences and progression are frequent. Therefore, adjuvant therapy should be considered. Resveratrol has shown potential as an adjuvant therapy for bladder cancer, with anti-proliferative effects at high concentrations and anti-angiogenic action at low concentrations. This review examines the standard therapeutic approach to bladder cancer, preclinical studies on resveratrol in bladder cancer xenotransplantation models, and discusses molecular signals, particularly the STAT3 pathway and angiogenic growth factor modulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Loris Riccardo Lopetuso, Claudia Cuomo, Irene Mignini, Antonio Gasbarrini, Alfredo Papa
Summary: Anti-tumour necrosis factor (TNF)-a agents are increasingly used for treating inflammatory bowel disease and various inflammatory disorders. However, the use of these drugs can sometimes lead to paradoxical reactions, which are still not well understood. In this study, we discuss the clinical aspects and overall outcomes of autoimmune diseases caused by anti-TNF-a therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Yuanyuan Kang, Ying Zhang, Yan Sun
Summary: miR-198 downregulation in OSCC tissues and cell lines is associated with poor overall survival and disease-free survival. Overexpression of miR-198 significantly inhibits proliferation, invasion, and EMT of OSCC cells. Luciferase assay results demonstrate that miR-198 interacts with CDK4 by directly targeting its binding site in the CDK4 sequence.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Jila Nasirzade, Zahra Kargarpour, Goran Mitulovic, Franz Josef Strauss, Layla Panahipour, Frank Schwarz, Reinhard Gruber
Summary: This study demonstrated that acid dentin lysate contains TGF-beta 1 and affects the expression of 231 regulated genes in gingival fibroblasts, with about one third of them being influenced by TGF-beta. The activation of canonical TGF-beta signaling was confirmed by the phosphorylation of Smad3 and translocation of Smad2/3 in response to ADL treatment. Additionally, TGF-beta released from dentin by acid lysis can adsorb to titanium and collagen membranes, suggesting a major response of gingival fibroblasts to dentin particles as a rich source of TGF-beta.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Anastasios Goulioumis, Kostis Gyftopoulos
Summary: Epithelial-to-mesenchymal transition (EMT) is a molecular phenomenon that occurs in epithelial neoplasms, such as laryngeal carcinoma. EMT leads to the loss of epithelial traits and acquisition of mesenchymal traits by tumor cells, enhancing their migratory capacity. EMT is mediated by complex molecular pathways and involves various changes, including loss of adhesion, cytoskeleton remodeling, evasion of apoptosis and immune surveillance, upregulation of metalloproteinases, etc. Partial EMT models have been accepted to explain the cell plasticity associated with invasion and metastasis of tumors.
Article
Oncology
Ulrich Krafft, Henning Reis, Marc Ingenwerth, Ilona Kovalszky, Markus Becker, Christian Niedworok, Christopher Darr, Peter Nyirady, Boris Hadaschik, Tibor Szarvas
PATHOLOGY & ONCOLOGY RESEARCH
(2020)
Article
Oncology
Katalin Kiss, Eszter Regos, Kristof Rada, Gabor Firneisz, Kornelia Baghy, Ilona Kovalszky
PATHOLOGY & ONCOLOGY RESEARCH
(2020)
Article
Oncology
Eszter Regos, Katalin Karaszi, Andrea Reszegi, Andras Kiss, Zsuzsa Schaff, Kornelia Baghy, Ilona Kovalszky
PATHOLOGY & ONCOLOGY RESEARCH
(2020)
Article
Oncology
Zsolt Mervai, Andrea Reszegi, Ildiko Miklya, Jozsef Knoll, Zsuzsa Schaff, Ilona Kovalszky, Kornelia Baghy
PATHOLOGY & ONCOLOGY RESEARCH
(2020)
Article
Biochemical Research Methods
Gabor Toth, Karoly Vekey, Simon Sugar, Ilona Kovalszky, Laszlo Drahos, Lilla Turiak
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Multidisciplinary Sciences
Alexandra Fullar, Katalin Karaszi, Peter Hollosi, Gabor Lendvai, Laszlone Olah, Andrea Reszegi, Zoltan Papp, Gabor Sobel, Jozsef Dudas, Ilona Kovalszky
Article
Andrology
Christian Niedworok, Carsten Kempkensteffen, Andreas Eisenhardt, Stephan Tschirdewahn, Christian Rehme, Andrej Panic, Henning Reis, Hideo Baba, Peter Nyirady, Boris Hadaschik, Ilona Kovalszky, Tibor Szarvas
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2020)
Article
Biochemistry & Molecular Biology
Peter Hollosi, Lorand Vancza, Katalin Karaszi, Katalin Dobos, Balint Peterfia, Eniko Tatrai, Peter Tatrai, Tibor Szarvas, Sandor Paku, Laszlo Szilak, Ilona Kovalszky
Article
Biochemistry & Molecular Biology
Andrea Reszegi, Zsolt Horvath, Katalin Karaszi, Eszter Regos, Victoria Postnikova, Peter Tatrai, Andras Kiss, Zsuzsa Schaff, Ilona Kovalszky, Kornelia Baghy
Article
Medicine, General & Internal
Csilla Olah, Stephan Tschirdewahn, Michele J. Hoffmann, Ulrich Krafft, Boris Hadaschik, Peter Nyirady, Attila Szendroi, Orsolya Modos, Anita Csizmarik, Ilona Kovalszky, Henning Reis, Tibor Szarvas
Article
Oncology
Andrea Reszegi, Katalin Karaszi, Gabor Toth, Kristof Rada, Lorand Vancza, Lilla Turiak, Zsuzsa Schaff, Andras Kiss, Laszlo Szilak, Gabor Szabo, Gabor Petovari, Anna Sebestyen, Katalin Dezso, Eszter Regos, Peter Tatrai, Kornelia Baghy, Ilona Kovalszky
Summary: Syndecan-1 plays a dual role in tumor development, either promoting or inhibiting tumors depending on the cancer type. In the context of liver cancer, transgenic expression of human syndecan-1 delayed tumor formation by downregulating lipid metabolism, inhibiting mTOR and beta-catenin pathways, and activating Foxo1 and p53 transcription factors. This oncoprotective effect was mediated by a beneficial modulation of lipid metabolism and molecular signaling pathways involved in cell cycle regulation and intermediary metabolism.
Article
Biochemical Research Methods
Gabor Toth, Domonkos Pal, Simon Sugar, Ilona Kovalszky, Katalin Dezso, Gitta Schlosser, Laszlo Drahos, Lilla Turiak
Summary: This study investigated the content and sulfation pattern of chondroitin sulfate (CS) and heparan sulfate (HS) in human hepatocellular carcinoma and cirrhotic liver tissues, considering the etiology of the diseases. Major differences were observed in the total abundance and sulfation pattern of CS and HS chains, providing important insights into the molecular mechanisms of chronic liver diseases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Orsolya Oravecz, Andrea Balogh, Roberto Romero, Yi Xu, Kata Juhasz, Zsolt Gelencser, Zhonghui Xu, Gaurav Bhatti, Roger Pique-Regi, Balint Peterfia, Petronella Hupuczi, Ilona Kovalszky, Padma Murthi, Adi L. Tarca, Zoltan Papp, Janos Matko, Nandor Gabor Than
Summary: Proteoglycan macromolecules, specifically syndecan-1, play crucial roles in various physiological processes during placenta development and healthy pregnancy. Dysregulated expression of proteoglycans, including syndecan-1, has been observed in pregnancy complications such as pre-eclampsia and fetal growth restriction. Further research is needed to explore the potential diagnostic and therapeutic applications of proteoglycans in complicated pregnancies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Simon Sugar, Fanni Bugyi, Gabor Toth, Judit Papay, Ilona Kovalszky, Tamas Tornoczky, Laszlo Drahos, Lilla Turiak
Summary: Tackling and curing cancer, especially lung cancer, is a crucial challenge in biomedical research. By analyzing the proteomics profile of different lung cancer subtypes, researchers aim to identify dysregulated pathways and improve diagnostic panels for lung cancer. This study successfully identified differentially expressed proteins in lung cancer tissue and distinguished distinct molecular profiles for different lung cancer types.
Article
Cell Biology
Bettina Ughy, Sarolta Nagyapati, Dezi B. Lajko, Tamas Letoha, Adam Prohaszka, Dima Deeb, Andras Der, Aladar Pettko-Szandtner, Laszlo Szilak
Summary: The growth of bacterial populations does not solely consist of continuous reproduction and cell death. Instead, there is a stationary phase during the growth of bacterial populations, which does not result from accumulated toxins or cell death. During the stationary phase, the phenotype of the cells changes while the total cell concentration remains constant, but the colony forming unit (CFU) decreases over time. Bacterial populations can be seen as virtual tissues formed through a specific differentiation process, where exponential-phase cells develop into stationary-phase cells and eventually become unculturable. The richness of nutrients does not affect the growth rate or stationary cell density. Generation time is not a constant value, but depends on the concentration of starter cultures. Inoculations with serial dilutions of stationary populations reveal a minimal stationary cell concentration (MSCC) point, below which cell concentrations remain constant upon dilution, which seems to be a universal phenomenon among unicellular organisms.