Article
Biochemistry & Molecular Biology
Ann-Kathrin Schmidt, Karoline Pudelko, Jan-Eric Boekenkamp, Katharina Berger, Maik Kschischo, Holger Bastians
Summary: Whole chromosome instability is a hallmark of human cancer and can be induced by abnormal microtubule assembly rates during mitosis, triggering errors in chromosome segregation. Loss of tumor suppressor genes can lead to increased mitotic microtubule assembly rates and contribute to chromosome instability in cancer cells.
Article
Biochemistry & Molecular Biology
Jih-Tung Pai, Ming-Wei Hsu, Yann-Lii Leu, Kuo-Ting Chang, Meng-Shih Weng
Summary: This study evaluated the anticancer activity of bavachinin on non-small cell lung cancer (NSCLC). Bavachinin induced G2/M cell cycle arrest in NSCLC cells by regulating cell cycle regulators and p21(Waf1/Cip1), which was reversed by inhibiting p38 MAPK activation.
Article
Multidisciplinary Sciences
Yang Zhang, Yujie Yang, Fan Yang, Xiaohan Liu, Ping Zhan, Jichao Wu, Xiaojie Wang, Ziying Wang, Wei Tang, Yu Sun, Yan Zhang, Qianqian Xu, Jin Shang, Junhui Zhen, Min Liu, Fan Yi
Summary: The upregulation of HDAC9 in fibrotic kidneys is found to promote epithelial cell cycle arrest at G2/M by regulating STAT1. Inhibition or knockdown of HDAC9 attenuates the loss of epithelial phenotype and fibroblasts activation, indicating its potential as a therapeutic target for kidney fibrosis.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Linnea Odborn Jonsson, Maryam Sahi, Ximena Lopez-Lorenzo, Faye Leilah Keller, Ourania N. Kostopoulou, Nikolas Herold, Lars Ahrlund-Richter, Shahrzad Shirazi Fard
Summary: In this study, a comprehensive analysis of cell cycle checkpoint activation following doxorubicin treatment was conducted in TP53 mutated ultra high-risk neuroblastoma cell lines, revealing distinct cell cycle checkpoints and potential druggable targets. The results showed dose-dependent accumulation in S- and/or G2/M-phase, as well as heterogeneous increase of cell cycle checkpoint proteins among the cell lines. Combination treatment with doxo and a small-molecule ATM inhibitor delayed regrowth in specific cell lines, indicating checkpoint dependent and independent targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Isabel de Pedro, Jesus Galan-Vidal, Ana Freije, Ernesto de Diego, Alberto Gandarillas
Summary: p21 plays a crucial role in driving keratinocytes towards differentiation in response to genomic stress by intervening in the mitosis block. Loss of p21 allows cells to escape differentiation and continue proliferation, while also driving keratinocytes from differentiation to apoptosis.
Article
Biochemistry & Molecular Biology
Anh Phong Tran, Christopher J. Tralie, Jose Reyes, Caroline Moosmuller, Zehor Belkhatir, Ioannis G. Kevrekidis, Arnold J. Levine, Joseph O. Deasy, Allen R. Tannenbaum
Summary: In this study, the time evolution of p21 and p53 in retinal pigment epithelial cells exposed to different levels of radiation was analyzed to investigate the effect of radiation on cell cycle arrest. Results showed that p21 levels, along with p53 levels to a lesser extent, had an impact on cell cycle arrest and the frequency of mitosis events.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Oncology
Shaomin Shi, Chongyang Li, Yanli Zhang, Chaowei Deng, Mengqin Tan, Guangzhao Pan, Juan Du, Yacong Ji, Qian Li, Hanghua Liang, Wei Liu, Leiyang Guo, Gaichao Zhao, Yaling Liu, Hongjuan Cui
Summary: The active ingredient Lycorine hydrochloride sourced from the medicinal herb Lycoris radiata has been shown to possess tumor suppression activity in various human cancers. In melanoma, an increased expression of p21(Cip1/WAF1) was found in tumor tissues and correlated with tumor invasion depth. Functional experiments demonstrated that LH effectively inhibited the proliferation, migration, and invasion ability of melanoma cells by inducing S phase cell cycle arrest and regulating epithelial-mesenchymal transition (EMT).
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Food Science & Technology
Yuko Murase Hetrick, Meseret Fesseha, Mee Young Hong
Summary: This study showed that watermelon powder and L-arginine supplementation can reduce the risk of colorectal cancer by upregulating gene expression of cell cycle regulators, leading to decreased proliferation and inhibition of aberrant crypt foci formation in all anatomical regions of the colon.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Biochemistry & Molecular Biology
Dina Hany, Marloes Zoetemelk, Kaushik Bhattacharya, Patrycja Nowak-Sliwinska, Didier Picard
Summary: Breast cancer is a persistent threat to women worldwide, and current monotherapies often face issues of drug resistance and toxicity. By mining data and constructing a network, we identified two multidrug combinations with high therapeutic relevance for ER alpha positive/HER2-/PI3K alpha mutant subtype of breast cancer.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Mayank Maheshwari, Nisha Yadav, Mohammad Hasanain, Praveen Pandey, Rohit Sahai, Kuldeep Choyal, Akhilesh Singh, Mushtaq A. Nengroo, Krishan K. Saini, Deepak Kumar, Kalyan Mitra, Dipak Datta, Jayanta Sarkar
Summary: The article discusses the dual function of p21 in cancer, with both inhibitory and oncogenic effects. Specifically, p21 negatively regulates basal autophagy and has oncogenic activity during early tumor development, while its inhibition promotes survival and growth of established tumors.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Yi Peng, Haoran Feng, Changgang Wang, Zijia Song, Yaqi Zhang, Kun Liu, Xi Cheng, Ren Zhao
Summary: ETV5 promotes tumor growth by inhibiting transcription of p21, while also altering drug sensitivity to palbociclib and dinaciclib. Therapeutic regimens targeting ETV5 may improve the efficacy of target-CDK treatment in CRC.
CELL DEATH & DISEASE
(2021)
Review
Cell Biology
Md Gulam Musawwir Khan, Yi Wang
Summary: This review summarizes the regulation of the cell cycle and cell-cycle-associated proteins by low-dose ionizing radiation (LDIR) and discusses the conceptual function and possible dichotomous role of p21(Waf1) in response to LDIR.
Article
Multidisciplinary Sciences
Qi Zhang, Yangchen Guo, Moorim Kang, Wei-Hsiang Lin, Jian-Cheng Wu, Ying Yu, Long-Cheng Li, Aimin Sang
Summary: The study suggests that p21 saRNA can be a potential therapeutic approach for PVR by inhibiting cell proliferation and migration of RPE cells, leading to the prevention of PVR progression and reducing postoperative failure rates.
Article
Biochemistry & Molecular Biology
Raul Garcia-Gonzalez, Eva Monte-Serrano, Patricia Morejon-Garcia, Elena Navarro-Carrasco, Pedro A. Lazo
Summary: This study reveals the role of VRK1 chromatin kinase in the activation of Tip60 and the cooperation between VRK1 and DNA-PK in Tip60 phosphorylation. T158 phosphorylation protects Tip60 from degradation, promotes its recruitment to chromatin, and is necessary for its full activity. S199 phosphorylation directly facilitates Tip60 autoacetylation. These findings are important for understanding the regulatory mechanisms of chromatin remodeling in the DNA damage response.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2022)
Article
Biochemistry & Molecular Biology
Jia-feng Tang, Guo-li Li, Tao Zhang, Yu-mei Du, Shi-ying Huang, Jian-hua Ran, Jing Li, Di-long Chen
Summary: In this study, it was found that HHT effectively inhibits the proliferation of melanoma cells by inducing DNA damage, apoptosis, and cell cycle arrest. Both in vitro and in vivo experiments confirmed the inhibitory effect of HHT on melanoma, providing evidence for its potential as an anti-melanoma agent.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Microbiology
Hye Seon Lee, Hye-Yeoung Yun, Eun-Woo Lee, Ho-Chul Shin, Seung Jun Kim, Bonsu Ku
Summary: The high-risk genotypes of human papillomaviruses (HPVs) are associated with cellular hyperproliferation abnormalities, including cervical cancer. In this study, it was demonstrated for the first time that the PDZ domain of human PTPN4 directly interacts with the PBM of HPV16 E6, providing structural and biochemical evidence for this interaction.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Cell Biology
Ara Jo, Jin Hee Bae, Yu Jeong Yoon, Tae Hun Chung, Eun-Woo Lee, Young-Ho Kim, Hea Min Joh, Jin Woong Chung
Summary: This study reveals that plasma-activated medium (PAM) promotes cell death in human lung cancer cells through the induction of ferroptosis. PAM increases intracellular and lipid ROS, leading to mitochondrial dysfunction, which can be protected by ROS scavengers and ferroptosis inhibitors.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Sang Bae Lee, Luciano Garofano, Aram Ko, Fulvio D'Angelo, Brulinda Frangaj, Danika Sommer, Qiwen Gan, KyeongJin Kim, Timothy Cardozo, Antonio Iavarone, Anna Lasorella
Summary: This study reveals the interaction mechanism between the anaphase-promoting complex (APC) and the tissue-specific transcription factor ID2 during mitosis. It shows that phosphorylation plays a crucial role in regulating the association between ID2 and the core APC, thus affecting post-mitotic transcription.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Sinai Kim, Wonhyo Lee, Huiju Jo, Seong-Keun Sonn, Se-Jin Jeong, Seungwoon Seo, Joowon Suh, Jing Jin, Hyae Yon Kweon, Tae Kyeong Kim, Shin Hye Moon, Sejin Jeon, Jong Woo Kim, Yu Ri Kim, Eun-Woo Lee, Hwa Kyoung Shin, Sung Ho Park, Goo Taeg Oh
Summary: This study discovered a novel type of microglia, called stroke-associated microglia (SAM), which is specifically activated in response to stroke-induced brain injury. SAM possesses enhanced antioxidant function and similar markers to disease-associated microglia (DAM). The expression of Peroxiredoxin-1 (Prdx1) is required for the activation and enhanced antioxidant function of SAM.
Article
Biochemistry & Molecular Biology
Seung Un Seo, Seon Min Woo, Min Wook Kim, Eun-Woo Lee, Kyoung-jin Min, Taeg Kyu Kwon
Summary: Raptor plays a critical role in mTORC1 signaling. OTUB1 stabilizes Raptor in a non-canonical manner, and the tyrosine 26 residue of OTUB1 is crucial for the interaction between OTUB1 and Raptor. Non-receptor tyrosine kinases induce phosphorylation of OTUB1 at Y26, leading to Raptor stabilization. Dephosphorylation of OTUB1 destabilizes Raptor and sensitizes cancer cells to drugs via mitochondrial dysfunction. Phospho-OTUB1 and Raptor are highly expressed in renal clear carcinoma samples, suggesting that targeting OTUB1 phosphorylation could be an effective therapeutic strategy for cancer treatment.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Tingting Bu, Jing Lan, Inseong Jo, Jie Zhang, Xue Bai, Shanru He, Xiaoling Jin, Lulu Wang, Yu Jin, Xiaoyu Jin, Liying Zhang, Hailong Piao, Nam-Chul Ha, Chunshan Quan, Ki Hyun Nam, Yongbin Xu
Summary: This study discovered that the pathogen Fusobacterium nucleatum, which is associated with destructive periodontal disease and colorectal cancer, has four genes that produce hydrogen sulfide, which helps protect against oxidative stress. By examining the structure and conducting inhibitor screening, two natural compounds were found to selectively inhibit the production of hydrogen sulfide by Fn1419. This research revealed the molecular mechanism of Fn1419 and identified a new potential inhibitor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Jinwook Lee, Inseong Jo, Ae-Ran Kwon, Nam-Chul Ha
Summary: Endoribonuclease YbeY specifically targets single-stranded RNA in ribosomal RNAs and small RNAs. It plays crucial roles in the maturation and quality control of ribosomal RNA in a wide range of bacteria and in the virulence of certain pathogenic bacteria. The crystal structure of YbeY from Staphylococcus aureus was determined, revealing a zinc ion at the active site and two molecules of tricarboxylic acid citrate. Molecular comparisons and a model of YbeY in complex with a single strand of RNA were constructed, providing insights into the molecular mechanism of how the YbeY enzyme recognizes single-stranded RNA in bacteria.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Oncology
Aram Ko, Mohammad Hasanain, Young Taek Oh, Fulvio D'Angelo, Danika Sommer, Brulinda Frangaj, Suzanne Tran, Franck Bielle, Bianca Pollo, Rosina Paterra, Karima Mokhtari, Rajesh Kumar Soni, Matthieu Peyre, Marica Eoli, Laura Papi, Michel Kalamarides, Marc Sanson, Antonio Iavarone, Anna Lasorella
Summary: LZTR1 is a substrate-specific adaptor of a CUL3-dependent ubiquitin ligase frequently mutated in cancer. The study identified EGFR and AXL as LZTR1 substrates targeted for degradation, and found that LZTR1 mutations result in the accumulation and deregulated signaling of EGFR and AXL. The study also revealed vulnerabilities of LZTR1-mutant tumors to inhibition of EGFR and AXL, providing precision targeting for patients with LZTR1-mutant cancer.
Article
Endocrinology & Metabolism
Da Som Lee, Tae Hyeon An, Hyunmi Kim, Eunsun Jung, Gyeonghun Kim, Seung Yeon Oh, Jun Seok Kim, Hye Jin Chun, Jaeeun Jung, Eun-Woo Lee, Baek-Soo Han, Dai Hoon Han, Yong-Ho Lee, Tae-Su Han, Keun Hur, Chul-Ho Lee, Dae-Soo Kim, Won Kon Kim, Jun Won Park, Seung-Hoi Koo, Je Kyung Seong, Sang Chul Lee, Hail Kim, Kwang-Hee Bae, Kyoung-Jin Oh
Summary: This study found that the expression of TCF7L2 in the liver affects hepatic lipid metabolism, and loss of TCF7L2 in the liver leads to excessive glycogen metabolism, promoting fatty acid accumulation and development of non-alcoholic fatty liver disease (NAFLD). Thus, TCF7L2 could be a potential regulator of NAFLD associated with high-carbohydrate diets and diabetes.
Article
Multidisciplinary Sciences
Min-Sik Lee, Courtney Dennis, Insia Naqvi, Lucas Dailey, Alireza Lorzadeh, George Ye, Tamara Zaytouni, Ashley Adler, Daniel S. Hitchcock, Lin Lin, Megan T. Hoffman, Aladdin M. Bhuiyan, Jaimie L. Barth, Miranda E. Machacek, Mari Mino-Kenudson, Stephanie K. Dougan, Unmesh Jadhav, Clary B. Clish, Nada Y. Kalaany
Summary: This study reveals the distinct dependence of pancreatic ductal adenocarcinoma (PDA) on de novo ornithine synthesis from glutamine, mediated by ornithine aminotransferase (OAT), which is required for tumor growth and polyamine synthesis. The reliance on OAT-mediated ornithine synthesis in PDA provides a potential therapeutic window for treating pancreatic cancer with minimal toxicity.
Editorial Material
Biochemistry & Molecular Biology
Ji-Yoon Lee, Jong Woo Kim, Eun-Woo Lee
NATURE CHEMICAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Anna Park, Kwang-eun Kim, Isaac Park, Sang Heon Lee, Kun-Young Park, Minkyo Jung, Xiaoxu Li, Maroun Bou Sleiman, Su Jeong Lee, Dae-Soo Kim, Jaehoon Kim, Dae-Sik Lim, Eui-Jeon Woo, Eun Woo Lee, Baek Soo Han, Kyoung-Jin Oh, Sang Chul Lee, Johan Auwerx, Ji Young Mun, Hyun-Woo Rhee, Won Kon Kim, Kwang-Hee Bae, Jae Myoung Suh
Summary: LETMD1 is a mitochondrial matrix protein that is crucial for maintaining respiratory function and thermogenesis in brown adipose tissue (BAT) upon cold stimulus. Knockout of LETMD1 leads to deficiency in OXPHOS complex proteins and impaired mitochondrial respiration in BAT. BAT-specific Letmd1 deficient mice exhibit the same phenotypes as Letmd1 knockout mice.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Jong Woo Kim, Min-Ju Kim, Tae-Hee Han, Ji-Yoon Lee, Sangok Kim, Hyerin Kim, Kyoung-Jin Oh, Won Kon Kim, Baek-Soo Han, Kwang-Hee Bae, Hyun Seung Ban, Soo Han Bae, Sang Chul Lee, Haeseung Lee, Eun-Woo Lee
Summary: By analyzing genomic and transcriptomic data from human cancer cell lines and tissue samples, we discovered that KEAP1 mutations and NRF2 activation are strongly associated with ferroptosis resistance in non-small cell lung carcinoma (NSCLC). We also identified AIFM2 as the gene most significantly correlated with ferroptosis resistance, followed by multiple NRF2 targets. Furthermore, we found that simultaneous inhibition of FSP1 and NRF2 induces ferroptosis more intensely.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Dong Geun Hong, Seulah Lee, Jaehoon Kim, Seonguk Yang, Myunggyo Lee, Jinsook Ahn, Haeseung Lee, Seung-Cheol Chang, Nam-Chul Ha, Jaewon Lee
Summary: The present study found that a diet containing morin can prevent motor dysfunction and dopaminergic neuronal damage in a mouse model of Parkinson's disease. It can also significantly reduce neuroinflammation. In vitro studies showed that morin exerts its anti-inflammatory effects by blocking the ERK-p65 pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Nayeon Ki, Inseong Jo, Yongseong Hyun, Jinwook Lee, Nam-Chul Ha, Hyun-Myung Oh
Summary: This study determines the crystal structure of the putative N-acetyltransferase of phage SPN3US and reveals its novel homodimeric arrangement. Further biochemical analyses demonstrate that the enzyme has a very narrow substrate specificity, highlighting the need for additional studies to elucidate its biochemical activity and the interaction between phage and host bacteria.
JOURNAL OF MICROBIOLOGY
(2022)