Journal
CELL DEATH AND DIFFERENTIATION
Volume 19, Issue 10, Pages 1655-1663Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/cdd.2012.47
Keywords
alcohol; apoptosis; Drosophila
Categories
Funding
- National Institute on Alcohol Abuse and Alcoholism [R01AA017328]
- National Institute of General Medical Sciences [R01GM072124]
- Welch Foundation [I-1727]
- [R01AA019526]
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Heavy alcohol consumption provokes an array of degenerative pathologies but the signals that couple alcohol exposure to regulated forms of cell death are poorly understood. Using Drosophila as a model, we genetically establish that the severity of ethanol challenge dictates the type of death that occurs. In contrast to responses seen under acute exposure, cytotoxic responses to milder challenges required gene encoding components of the apoptosome, Dronc and Dark. We conducted a genome-wide RNAi screen to capture targets that specifically mediate ethanol-induced cell death. One effector, Drat, encodes a novel protein that contains an ADH domain but lacks essential residues in the catalytic site. In cultured cells and neurons in vivo, depletion of Drat conferred protection from alcohol-induced apoptosis. Adults mutated for Drat showed both improved survival and enhanced propensities toward sedation after alcohol challenge. Together, these findings highlight novel effectors that support regulated cell death incited by alcohol stress in vitro and in vivo. Cell Death and Differentiation (2012) 19, 1655-1663; doi:10.1038/cdd.2012.47; published online 27 April 2012
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