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Cell Biology
Lin-Lin Hu, Meng-Hao Pan, Feng-Lian Yang, Zi-Ao Zong, Feng Tang, Zhen-Nan Pan, Xiang Lu, Yan-Ping Ren, Jun-Li Wang, Shao-Chen Sun
Summary: Research demonstrates that FASCIN is crucial for mouse oocyte maturation, affecting spindle migration and polar body extrusion. It may be regulated by PKC and plays a significant role in meiosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aleksandar Mihajlovic, Candice Byers, Laura Reinholdt, Greg Fitzharris
Summary: Chromosome segregation errors in mammalian oocyte meiosis, including SAC ineffectiveness in meiosis-II, contribute to age-related aneuploidy.
Article
Cell Biology
Zhen-Nan Pan, Jing-Cai Liu, Jia-Qian Ju, Yue Wang, Shao-Chen Sun
Summary: This study demonstrates the critical roles of LRRK2 in mammalian oocyte meiosis, primarily through regulating actin assembly for spindle migration and maintaining mitochondrial function.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jia-Qian Ju, Zhen-Nan Pan, Kun-Huan Zhang, Yi-Ming Ji, Jing-Cai Liu, Shao-Chen Sun
Summary: This study demonstrates that Mcrs1 plays important roles in regulating the meiotic G2/M transition and spindle assembly in mouse oocytes. Mcrs1 is present in the nucleus and associates with spindle poles and chromosomes during meiosis I. Depletion of Mcrs1 affects histone modifications, CDK1 activity, and cyclin B1 accumulation, leading to delayed G2/M transition and abnormal spindle assembly.
Article
Cell Biology
Hong Yin, Teng Zhang, Hao Wang, Xin Hu, Xuan Hou, Xianbao Fang, Yaoxue Yin, Hui Li, Lanying Shi, You-Qiang Su
Summary: The research reveals that EML1 plays a crucial role in controlling oocyte meiotic progression in mice, and its loss leads to abnormal oocytes unable to progress to the second meiotic division.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Qingqing Cai, Keying Wen, Miao Ma, Wei Chen, Delin Mo, Zuyong He, Yaosheng Chen, Peiqing Cong
Summary: EZH2 plays a crucial role in porcine oocyte meiotic maturation by regulating spindle organization, maintaining chromosomal integrity, and mitochondrial function.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Feng Dong, Tie-Gang Meng, Jian Li, Feng Wang, Yuan-Yuan Li, Ying-Chun Ouyang, Yi Hou, Zhen-Bo Wang, Heide Schatten, Qing-Yuan Sun
Summary: The Cyclin D-CDK4/6 complex plays a crucial role in regulating the spindle assembly checkpoint (SAC) to prevent aneuploidy in meiosis I in oocytes. Inhibition of CDK4/6 kinases disrupts spindle assembly and chromosome alignment, while also accelerating meiotic progression by inactivating SAC, resulting in the production of aneuploid oocytes. These findings highlight a novel role for Cyclin D-CDK4/6 complex in controlling SAC in female meiosis I.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Wei Yue, Yue Wang, Tie-Gang Meng, Hong-Yong Zhang, Xin-Ran Zhang, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhen-Bo Wang, Qing-Yuan Sun
Summary: This study investigates the role of outer kinetochore protein KNL1 in mouse oocytes and reveals its critical function in ensuring timely and accurate chromosome segregation during oocyte meiotic maturation.
Article
Cell Biology
Xingzhu Du, Jun Li, Qingrui Zhuan, Luyao Zhang, Lin Meng, Panyu Ren, Xiaohan Huang, Jiachen Bai, Pengcheng Wan, Wenquan Sun, Yunpeng Hou, Shien Zhu, Xiangwei Fu
Summary: Oocyte cryopreservation has significant benefits in conserving animal germplasm resources and assisting reproductive technology. However, this technique can cause damage to oocytes, leading to a decrease in oocyte quality and post-fertilization embryonic development. This study examined oocyte cortical tension and found that vitrified oocytes had decreased cortical tension. Increasing cortical tension improved oocyte polar body extrusion, embryo development, spindle positioning, KT-MT attachment, SAC activity, and reduced aneuploidy in vitrified oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Juan Ge, Na Zhang, Shoubin Tang, Feifei Hu, Xiaojing Hou, Hongzheng Sun, Longsen Han, Qiang Wang
Summary: Maternal diabetes impairs oocyte quality, but controlling the PDK1-mediated Ser232-PDHE1α phosphorylation pathway can partly protect oocytes from the effects of diabetes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Hao Qin, Yi Qu, Yi-Feng Yuan, Yang-Yang Li, Jie Qiao
Summary: This study revealed that RBM14 is down-regulated in oocytes from old mice and shows overlapped localization patterns with alpha-tubulin in different stages of meiosis. Knockdown of RBM14 may result in symmetric division of oocytes, spindle defects, and chromosome abnormalities. Additionally, RBM14 interacts with endogenous alpha-tubulin and modulates oocyte meiotic maturation by affecting alpha-tubulin acetylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Food Science & Technology
Cong Ma, Zhuonan Yang, Shouxin Zhang, Xueke Zhang, Siyuan Wang, Huiru Cheng, Yang Liu, Hongzhen Ruan, Zuying Xu, Chunmei Liang, Dan Liang, Zhiming Ding, Yajing Liu, Yunxia Cao
Summary: CBZ exposure negatively affects the maturation of mouse oocytes, leading to disruptions in polar body extrusion, cell cycle distribution, spindle assembly, chromosomal alignment, and kinetochore microtubule attachment.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Oncology
Xiao-Ming Liu, Fang Chen, Li Wang, Fan Zhang, Li-Jun Huo
Summary: In this study, the joint roles of Chk1/2 in oocyte meiotic maturation were examined, with Chk1/2 inhibition resulting in defective meiotic spindle organization and chromosome condensation. Interestingly, inhibiting Chk1 alone had different effects compared to Chk1/2 inhibition, indicating distinct functions of Chk1 and Chk2 during oocyte maturation. These findings provide further insights into the functions and regulatory mechanisms of Chk1/2 in oocyte meiotic maturation.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Reproductive Biology
Cecilia S. Blengini, Karen Schindler
Summary: This article discusses the basic mechanisms of spindle formation during meiotic maturation, focusing on studies in mice and humans. It also explores various factors that may influence spindle formation and stability, as well as the potential impact of assisted reproductive technologies on spindles and subsequent embryo development.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
Ying Xu, Chang -Long Xu, Zhong-Feng Xu, Xin-Jie Wang, Hui-Sheng Liang, Zhao-Cheng Zeng, Li-Xin Zeng, Kang-Na Wei, Shu-Zi Deng, Shu-Juan Xie, Jiang Jiang, Yu-Xin Liu, Yun-Kao Cao, Hai-Long Wang
Summary: Fbf1 plays a crucial role in spindle assembly and chromosome arrangement during mouse oocyte meiosis, affecting the progression of cell cycle.
Article
Biochemistry & Molecular Biology
Jianyu Wang, Renyue Wei, Gerelchimeg Bou, Zhonghua Liu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2015)
Article
Cell Biology
Yu Zhang, Hai Li, Renyue Wei, Jing Ma, Yanhua Zhao, Zhengxing Lian, Zhonghua Liu
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2015)
Article
Cell & Tissue Engineering
Yanjun Huan, Hongmei Wang, Zhanfeng Wu, Jiguang Zhang, Jiang Zhu, Zhonghua Liu, Hongbin He
CELLULAR REPROGRAMMING
(2015)
Article
Biochemistry & Molecular Biology
Xin Li, Xiao-Long Cui, Jia-Qiang Wang, Yu-Kai Wang, Yu-Fei Li, Le-Yun Wang, Hai-Feng Wan, Tian-Da Li, Gui-Hai Feng, Ling Shuai, Zhi-Kun Li, Qi Gu, Jie Hao, Liu Wang, Xiao-Yang Zhao, Zhong-Hua Liu, Xiu-Jie Wang, Wei Li, Qi Zhou
Article
Developmental Biology
Gerelchimeg Bou, Shichao Liu, Jia Guo, Yueming Zhao, Mingju Sun, Binghua Xue, Jiaqiang Wang, Yanchang Wei, Qingran Kong, Zhonghua Liu
DEVELOPMENTAL BIOLOGY
(2016)
Article
Cell Biology
Ruizhen Sun, Lei Lei, Shichao Liu, Binghua Xue, Jianyu Wang, Jiaqiang Wang, Jingling Shen, Lian Duan, Xinghui Shen, Yimei Cong, Yanli Gu, Kui Hu, Lianhong Jin, Zhong-hua Liu
Article
Agriculture, Dairy & Animal Science
Yuting Zhang, Hanlin Liang, Yan Liu, Meng Zhao, Qianqian Xu, Zhonghua Liu, Xiaogang Weng
Summary: The freezing process of boar sperm shows varying freezability between individuals. Analysis revealed specific markers in the metabolic level of seminal plasma that could potentially serve as indicators for assessing sperm cryopreservation resistance in boars.
Article
Biochemistry & Molecular Biology
Zhi Yin, Jing-Tao Sun, Hong-Di Cui, Chao-Qian Jiang, Yu-Ting Zhang, Sanghoon Lee, Zhong-Hua Liu, Jun-Xue Jin
Summary: The study showed that supplementation of 10 μg/mL tannic acid had beneficial effects on oocyte cytoplasmic maturation and subsequent embryonic development in pigs, while a high concentration of 100 μg/mL tannic acid might have negative impacts on cytoplasmic maturation and embryonic development.
Article
Biochemistry & Molecular Biology
Jun-Xue Jin, Jing-Tao Sun, Chao-Qian Jiang, Hong-Di Cui, Ya Bian, Sanghoon Lee, Lianjin Zhang, Byeong Chun Lee, Zhong-Hua Liu
Summary: This study reveals the role of melatonin receptors in lipid metabolism and redox signaling during in vitro cumulus-oocyte complex (COC) development, highlighting their potential to improve female reproductive health. Understanding the dynamics of interactions between lipid homeostasis and redox signaling driven by melatonin receptors is crucial for predicting drug targets and therapeutic effects.
Article
Biochemistry & Molecular Biology
Yang Yu, Xuechun Li, Yimei Li, Renyue Wei, Hai Li, Zhonghua Liu, Yu Zhang
Summary: This study establishes a stable iPSC-EC induction system and investigates the regulatory mechanism of endothelial differentiation. The derived piPSC-ECs form microvessel-like structures in vitro and promote blood vessel formation under pathological conditions. The activation of TGF-beta-Smad2/3 signaling promotes mesoderm formation and prevents endothelial differentiation in this model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jing-Tao Sun, Jin-Dong Yuan, Qi Zhang, Xin Luo, Xin-Yue Qi, Jia-Hui Liu, Xi-Qing Jiang, Sanghoon Lee, Anukul Taweechaipaisankul, Zhong-Hua Liu, Jun-Xue Jin
Summary: This study demonstrates that supplementation of 10(-11) M ramelteon during in vitro maturation (IVM) can significantly improve oocyte maturation and subsequent embryo development in pigs by reducing oxidative stress and maintaining lipid homeostasis.
Article
Biochemistry & Molecular Biology
Jing-Tao Sun, Jia-Hui Liu, Xi-Qing Jiang, Xin Luo, Jin-Dong Yuan, Qi Zhang, Xin-Yue Qi, Sanghoon Lee, Zhong-Hua Liu, Jun-Xue Jin
Summary: Tannin (TA) enhances porcine oocyte cytoplasmic maturation and subsequent embryonic development after in vitro fertilization (IVF), but the mechanism through which TA blocks polyspermy after IVF remains unclear. This study found that TA can improve the quality of fertilized oocytes and reduce the risk of polyspermic penetration by regulating organelles' functions and activities.
Article
Biochemistry & Molecular Biology
Qiao Liu, Liqun Yu, Ziwen Zhang, Yang Chang, Zhonghua Liu, Chunzhu Xu
Summary: Body size is an important trait that has received much attention. Small domestic pigs can be used as models for biomedicine and satisfy the cultural needs of sacrifice. Through gene co-expression network analysis, seven candidate genes associated with body size were identified, with most of their functions associated with lipid deposition. The study concluded that PLIN1 is an important genetic factor regulating lipid deposition and affecting body size variation in pigs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chaoqian Jiang, Lishuang Geng, Jinpeng Wang, Yingjuan Liang, Xiaochen Guo, Chang Liu, Yunjing Zhao, Junxue Jin, Zhonghua Liu, Yanshuang Mu
Summary: We developed a CRISPR-based platform that can perform four functions and target multiple genomic loci encoded in a single transcript. By fusing specific RNA hairpins with gRNA scaffolds and utilizing paired RNA-binding proteins, we achieved simultaneous and independent regulation of multiple target genes. This system provides a powerful tool for investigating synthetic biology questions and engineering complex-phenotype medical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Bingteng Xie, Heng Zhang, Renyue Wei, Qiannan Li, Xiaogang Weng, Qingran Kong, Zhonghua Liu