Journal
CELL CYCLE
Volume 10, Issue 20, Pages 3527-3532Publisher
LANDES BIOSCIENCE
DOI: 10.4161/cc.10.20.17792
Keywords
kinase; analog-sensitive; conditional allele; fission yeast; phosphorylation
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Funding
- Austrian Science Fund [P23609, F3403]
- Slovak Academic Information Agency
- European Community [FP7/2007-2013, PERG07-GA-2010-268167]
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The genome of the fission yeast Schizosaccharomyces pombe encodes for 17 protein kinases that are essential for viability. Studies of the essential kinases often require the use of mutant strains carrying conditional alleles. To inactivate these kinases conditionally, we applied a recently developed chemical-genetic strategy. The mutation of a single residue in the ATP-binding pocket confers sensitivity to small-molecule inhibitors, allowing for specific inactivation of the modified kinase. Using this approach, we constructed conditional analog-sensitive alleles of 13 essential protein kinases in the fission yeast S. pombe.
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