Article
Reproductive Biology
Congjing Wang, Meng Xi Chen, Yuan Zhang, Xue Bai, Qiqi Cao, Jian Han, Nana Zhang, Chun Zhao, Xiufeng Ling, Ximan Rui, Yichun Guan, Junqiang Zhang, Ran Huo
Summary: Mutations in the CCNB3 gene cause oocyte maturation arrest and infertility in females. The mutations affect the function of CDK1, a key protein in the cell cycle, resulting in arrested oocyte development. In vitro experiments show that the addition of specific inhibitors can improve the maturation rates of mutant oocytes.
JOURNAL OF OVARIAN RESEARCH
(2023)
Review
Plant Sciences
Marina de Lyra Soriano Saleme, Ingrid Rocha Andrade, Nubia Barbosa Eloy
Summary: Most eukaryotic species propagate through sexual reproduction involving male and female gametes. In flowering plants, this process begins with DNA replication and chromosome segregation, followed by mitotic divisions to form male and female gametophytes. Precise regulation of gametogenesis progression is essential for successful meiotic cell division and sexual differentiation in plant species.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Cell Biology
Tianyan Wang, Martuza Sarwar, Jonathan B. Whitchurch, Hilary M. Collins, Tami Green, Julius Semenas, Amjad Ali, Christopher J. Roberts, Ryan D. Morris, Madlen Hubert, Sa Chen, Zahra El-Schich, Anette G. Wingren, Thomas Grundstroem, Richard Lundmark, Nigel P. Mongan, Lena Gunhaga, David M. Heery, Jenny L. Persson
Summary: This study identifies the role of PIP5K1α in promoting the growth and invasion of castration-resistant prostate cancer. The study also reveals the importance of the N-terminal domain of PIP5K1α and its function as an upstream regulator of androgen receptor and its target genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Puja Chakraborty, Ratnadip Paul, Abhishek Chowdhury, Nayonika Mukherjee, Somsubhra Nath, Susanta Roychoudhury
Summary: This study investigates the role of histone ubiquitylation in transcription regulation, particularly the mono-ubiquitylation of histone 2B by RING finger motif-containing ubiquitin ligases. The study shows that the anaphase-promoting complex/cyclosome (APC/C), along with its adapter protein Cdc20, catalyses the mono-ubiquitylation of Lysine-120 on the UBCH10 promoter. The study also uncovers a cell-cycle-specific pattern of this modification and suggests a crosstalk between acetylation and ubiquitylation in UBCH10 trans-regulation.
Article
Cardiac & Cardiovascular Systems
Kyuho Jeong, James M. Murphy, Eun-Young Erin Ahn, Ssang-Taek Steve Lim
Summary: This study shows that FAK regulates the expression of CDK inhibitors p27 and p21 in vascular smooth muscle cells (VSMCs) by reducing the stability of Skp2. FAK interacts with Skp2 and Fzr1 in the nucleus, promoting their ubiquitination and degradation. Inhibition of FAK leads to reduced Skp2 expression and elevated levels of p27 and p21, resulting in decreased intimal hyperplasia.
CARDIOVASCULAR RESEARCH
(2022)
Review
Cell Biology
Alex Willems, Lieven De Veylder
Summary: The review focuses on the plant APC/C complex, discussing its importance in plant development and its association with different developmental processes. The interactions between plant-specific APC/C activator subunits and other subunits are also described. Finally, a comprehensive list of confirmed and suspected plant APC/C target proteins is provided, offering opportunities to manipulate APC/C activity for increased crop yield and improved resilience to climate change.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Eyashin Ali, Manash Jyoti Kalita, Simanta Kalita, Jayasree Talukdar, Ankur Jyoti Deka, Jasmin Sultana, Bikash Narayan Choudhury, Munindra Narayan Baruah, Sahana Bhattacharjee, Subhash Medhi
Summary: Esophageal squamous cell carcinoma (ESCC) is a prevalent type of esophageal cancer worldwide with a poor prognosis. Dysregulation of anaphase promoting complex/cyclosome (APC/C) activation due to altered expression of APC molecules may contribute to the development of ESCC. The study analyzed the expression of APC/C complex subunits and found significant upregulation of APC7 and APC3, which suggested their role in uncontrolled cell proliferation in ESCC. Additionally, lifestyle factors and their association with APC expression were also studied.
Review
Cell Biology
Tatyana Bodrug, Kaeli A. Welsh, Megan Hinkle, Michael J. Emanuele, Nicholas G. Brown
Summary: The ubiquitin-proteasome system plays a crucial role in cell cycle regulation, neurobiology, and chromatin control. Recent studies have revealed new mechanisms of APC/C activity and its involvement in broader signaling pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Oncology
Johannes Tobias Thiel, Adrien Daigeler, Jonas Kolbenschlag, Katarzyna Rachunek, Sebastian Hoffmann
Summary: Soft tissue sarcomas (STSs) are rare malignant conditions that are difficult to treat. Recent research indicates that dysregulation of cyclin-dependent kinase (CDK) signaling pathways can be a strong driver of sarcogenesis. This review discusses the role of CDK signaling in STS development and its implications for targeted treatment.
Review
Cell Biology
Aleksandra J. Pluta, Cecilia Studniarek, Shona Murphy, Chris J. Norbury
Summary: This article discusses the important roles of structurally related cyclin-dependent protein kinases (CDKs) in eukaryotic cell function, highlighting their roles in cell cycle regulation and transcriptional regulation, and the overlap between these processes.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Cell Biology
Jing Liu, Yunhua Peng, Le Shi, Lixin Wan, Hiroyuki Inuzuka, Jiangang Long, Jianping Guo, Jinfang Zhang, Min Yuan, Shuangxi Zhang, Xun Wang, Jing Gao, Xiangpeng Dai, Shozo Furumoto, Lijun Jia, Pier Paolo Pandolfi, John M. Asara, William G. Kaelin, Jiankang Liu, Wenyi Wei
Summary: Glucose metabolism in mammalian cells varies with different cell cycle phases, with TCA cycle being predominantly used in G1 phase and glycolysis favored in S phase. The coupling of cell cycle with metabolism is largely achieved through Skp2-mediated timely destruction of key TCA cycle enzymes IDH1/2. This mechanistic link between aberrant cell cycle and glycolysis addiction provides insights into the coupling of metabolic fluctuation with periodic cell cycle in mammalian cells.
Article
Plant Sciences
Rodrigo Porto Schwedersky, Marina de Lyra Soriano Saleme, Ingrid Andrade Rocha, Patricia da Fonseca Montessoro, Adriana Silva Hemerly, Nubia Barbosa Eloy, Paulo Cavalcanti Gomes Ferreira
Summary: The study reveals the crucial role of APC11 in early plant development, mediating overall plant growth by regulating APC/C activity.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Pan Gong, Michiel Bontinck, Kirin Demuynck, Jolien De Block, Kris Gevaert, Dominique Eeckhout, Geert Persiau, Stijn Aesaert, Griet Coussens, Mieke Van Lijsebettens, Laurens Pauwels, Geert De Jaeger, Dirk Inze, Hilde Nelissen
Summary: SAMBA has been identified as a plant-specific regulator of the anaphase-promoting complex/cyclosome (APC/C) in Arabidopsis and maize. Two samba genome edited mutants in maize showed growth defects, with samba-1 representing a knockout allele and samba-3 producing a truncated protein that still interacted with APC/C but with altered activity. This suggests a dosage-dependent role for SAMBA in controlling developmental processes.
Article
Oncology
Owen J. Chen, Ester Castellsague, Mohamed Moustafa-Kamal, Javad Nadaf, Barbara Rivera, Somayyeh Fahiminiya, Yilin Wang, Isabelle Gamache, Caterina Pacifico, Lai Jiang, Jian Carrot-Zhang, Leora Witkowski, Albert M. Berghuis, Stefan Schoenberger, Dominik Schneider, Morten Hillmer, Susanne Bens, Reiner Siebert, Colin J. R. Stewart, Ziguo Zhang, William C. H. Chao, Celia M. T. Greenwood, David Barford, Marc Tischkowitz, Jacek Majewski, William D. Foulkes, Jose G. Teodoro
Summary: Two germline CDC20 missense variants that segregate with cancer in two families compromise the spindle assembly checkpoint and lead to aberrant mitotic progression, which could predispose cells to transformation.
Review
Biochemistry & Molecular Biology
Pawel Lukasik, Irena Baranowska-Bosiacka, Katarzyna Kulczycka, Izabela Gutowska
Summary: Recent studies have shown that small molecule drugs targeting CDK inhibitors have become attractive options for treating cancer and neurodegenerative disorders. Most CDK inhibitors target the ATP binding pocket, but the similarity in structure among CDK kinases makes achieving selectivity challenging. Therefore, inhibitors binding outside the ATP binding site have attracted great interest for their potential applications in the biomedical field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hui Wang, Hui Shi, Malini Rajan, Elizabeth R. Canarie, Seoyeon Hong, Daniele Simoneschi, Michele Pagano, Matthew F. Bush, Stefan Stoll, Elizabeth A. Leibold, Ning Zheng
Article
Multidisciplinary Sciences
Martin Reynders, Bryan S. Matsuura, Marleen Berouti, Daniele Simoneschi, Antonio Marzio, Michele Pagano, Dirk Trauner
Editorial Material
Biochemistry & Molecular Biology
Antonio Marzio, Michele Pagano
CELL DEATH AND DIFFERENTIATION
(2020)
Article
Cell Biology
Andras Horvath, Gergely Rona, Michele Pagano, Philip W. Jordan
BMC MOLECULAR AND CELL BIOLOGY
(2020)
Article
Cell Biology
Takaaki Tsunematsu, Rieko Arakaki, Hidehiko Kawai, Jan Ruppert, Koichi Tsuneyama, Naozumi Ishimaru, William C. Earnshaw, Michele Pagano, Yasusei Kudo
JOURNAL OF CELL SCIENCE
(2020)
Article
Cell Biology
Loredana Moro, Daniele Simoneschi, Emma Kurz, Arnaldo A. Arbini, Shaowen Jang, Nicoletta Guaragnella, Sergio Giannattasio, Wei Wang, Yu-An Chen, Geoffrey Pires, Andrew Dang, Elizabeth Hernandez, Payal Kapur, Ankita Mishra, Aristotelis Tsirigos, George Miller, Jer-Tsong Hsieh, Michele Pagano
NATURE CELL BIOLOGY
(2020)
Article
Biology
Hajnalka L. Palinkas, Angela Bekesi, Gergely Rona, Lorinc Pongor, Gabor Papp, Gergely Tihanyi, Eszter Holub, Adam Poti, Carolina Gemma, Simak Ali, Michael J. Morten, Eli Rothenberg, Michele Pagano, David Szuts, Balazs Gyorffy, Beata G. Vertessy
Editorial Material
Cell Biology
Luca Lignitto, Michele Pagano
Summary: Cell-cell fusion, crucial for the development of multicellular organisms, is driven by the remodeling of the actin cytoskeleton. A recent study reveals how CRL3-dependent mono-ubiquitylation regulates cell fusion by controlling the dynamics of cytoskeletal rearrangements.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Marco Salamina, Bailey C. Montefiore, Mengxi Liu, Daniel J. Wood, Richard Heath, James R. Ault, Lan-Zhen Wang, Svitlana Korolchuk, Arnaud Basle, Martyna W. Pastok, Judith Reeks, Natalie J. Tatum, Frank Sobott, Stefan T. Arold, Michele Pagano, Martin E. M. Noble, Jane A. Endicott
Summary: The SCFSKP2 ubiquitin ligase promotes degradation of p27KIP1 to relieve G1 checkpoint control of CDK-cyclin complexes, ensuring orderly cell cycle progression. The interaction between SKP2 and cyclin A provides a structural mechanism for fine-tuning the degradation of p27KIP1 and distinguishes cyclin A from other G1 cyclins.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Emiliano Maiani, Giacomo Milletti, Francesca Nazio, Sos Gronbaek Holdgaard, Jirina Bartkova, Salvatore Rizza, Valentina Cianfanelli, Mar Lorente, Daniele Simoneschi, Miriam Di Marco, Pasquale D'Acunzo, Luca Di Leo, Rikke Rasmussen, Costanza Montagna, Marilena Raciti, Cristiano De Stefanis, Estibaliz Gabicagogeascoa, Gergely Rona, Nelida Salvador, Emanuela Pupo, Joanna Maria Merchut-Maya, Colin J. Daniel, Marianna Carinci, Valeriana Cesarini, Alfie O'sullivan, Yeon-Tae Jeong, Matteo Bordi, Francesco Russo, Silvia Campello, Angela Gallo, Giuseppe Filomeni, Letizia Lanzetti, Rosalie C. Sears, Petra Hamerlik, Armando Bartolazzi, Robert E. Hynds, David R. Pearce, Charles Swanton, Michele Pagano, Guillermo Velasco, Elena Papaleo, Daniela De Zio, Apolinar Maya-Mendoza, Franco Locatelli, Jiri Bartek, Francesco Cecconi
Summary: AMBRA1 has been identified as a key regulator in cell cycle control, playing a crucial role in maintaining genomic integrity by regulating the abundance of D-type cyclins and facilitating the transition from G1 to S phase, thereby preventing replication stress. The findings suggest that CHK1 kinase could be a potential therapeutic target in AMBRA1-deficient tumors, highlighting the importance of the AMBRA1-cyclin D pathway in regulating cell cycle and genomic stability in embryonic development and tumorigenesis.
Article
Multidisciplinary Sciences
Daniele Simoneschi, Gergely Rona, Nan Zhou, Yeon-Tae Jeong, Shaowen Jiang, Giacomo Milletti, Arnaldo A. Arbini, Alfie O'Sullivan, Andrew A. Wang, Sorasicha Nithikasem, Sarah Keegan, Yik Siu, Valentina Cianfanelli, Emiliano Maiani, Francesca Nazio, Francesco Cecconi, Francesco Boccalatte, David Fenyo, Drew R. Jones, Luca Busino, Michele Pagano
Summary: The research identifies AMBRA1 as a ubiquitin ligase that degrades D-type cyclins, whose loss leads to accumulation of D-type cyclins and tumorigenesis. Furthermore, AMBRA1 also affects the sensitivity to CDK4/6 inhibitors.
Article
Multidisciplinary Sciences
Elijah L. Mena, Callie J. Donahue, Laura Pontano Vaites, Jie Li, Gergely Rona, Colin O'Leary, Luca Lignitto, Bearach Miwatani-Minter, Joao A. Paulo, Avantika Dhabaria, Beatrix Ueberheide, Steven P. Gygi, Michele Pagano, J. Wade Harper, Robert A. Davey, Stephen J. Elledge
Summary: The study found an interaction between ORF10 and CRL2ZYG11B but no evidence that ORF10 is functioning to inhibit or hijack CRL2ZYG11B.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Giang Thanh Nguyen-Dien, Keri-Lyn Kozul, Yi Cui, Brendan Townsend, Prajakta Gosavi Kulkarni, Soo Siang Ooi, Antonio Marzio, Nissa Carrodus, Steven Zuryn, Michele Pagano, Robert G. Parton, Michael Lazarou, S. Sean Millard, Robert W. Taylor, Brett M. Collins, Mathew J. K. Jones, Julia K. Pagan
Summary: To maintain mitochondrial quality and quantity, cells use mitophagy to selectively remove damaged or excessive mitochondria. SCFFBXL4, a protein ubiquitin ligase complex that localises to the mitochondrial outer membrane, plays a role in constitutive ubiquitylation and degradation of mitophagy receptors NIX and BNIP3, thus suppressing basal levels of mitophagy.
Article
Oncology
Jiehui Deng, Aatish Thennavan, Igor Dolgalev, Ting Chen, Jie Li, Antonio Marzio, John T. Poirier, David H. Peng, Mirna Bulatovic, Subhadip Mukhopadhyay, Heather Silver, Eleni Papadopoulos, Val Pyon, Cassandra Thakurdin, Han Han, Fei Li, Shuai Li, Hailin Ding, Hai Hu, Yuanwang Pan, Vajira Weerasekara, Baishan Jiang, Eric S. Wang, Ian Ahearn, Mark Philips, Thales Papagiannakopoulos, Aristotelis Tsirigos, Eli Rothenberg, Justin Gainor, Gordon J. Freeman, Charles M. Rudin, Nathanael S. Gray, Peter S. Hammerman, Michele Pagano, John V. Heymach, Charles M. Perou, Nabeel Bardeesy, Kwok-Kin Wong
Summary: The study found that LKB1-deficient lung tumors are sensitive to autophagy inhibition, which can restore impaired antigen presentation and antitumor immune responses, enhancing the effectiveness of PD-1 immunotherapy. Additionally, LKB1 deficiency inhibits antigen processing and presentation, but this can be reversed by targeting the autophagy pathway to restore immunoproteasome activity and antigen presentation.
Article
Oncology
Loredana Moro, Michele Pagano
MOLECULAR & CELLULAR ONCOLOGY
(2020)