Article
Immunology
Yanmeizhi Wu, Jingjing Li, Xu Yang, Boyu Hou, Hong Qiao
Summary: In a retrospective analysis, it was found that female gender, IgG, FT4, TPOAb, TGAb, TSHI, TFQI, and TSH are associated with thyroid immune-related adverse events (irAE) caused by anti-PD-1 treatment. In vitro experiments showed that anti-PD-1 downregulates AKT1-SKP2 to enhance thyroid immunosensitivity. This study reveals the mechanisms underlying anti-PD-1-associated thyroid immune injury.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yumeng Cui, Xiaojie Wu, Jie Jin, Weiling Man, Jie Li, Xiang Li, Yanghua Li, He Yao, Rongbin Zhong, Shiyun Chen, Jiahui Wu, Tianhao Zhu, Yanli Lin, Junjie Xu, Youliang Wang
Summary: In this study, circHERC1 was found to be significantly upregulated in lung cancer tissues and cells, and its ectopic expression promoted proliferation, invasion, and metastasis while inhibiting apoptosis of lung cancer cells both in vitro and in vivo. Mechanistic studies revealed that circHERC1 acted as a competitive endogenous RNA of miR-142-3p to relieve the suppressive effect on its target HMGB1, thus activating the MAPK/ERK and NF-kappa B pathways and promoting migration and invasion. Additionally, circHERC1 was found to sequester FOXO1 in the cytoplasm, regulating AKT activity and BIM transcription, which led to increased cell proliferation and decreased apoptosis.
Article
Biochemistry & Molecular Biology
Li-Xiang Wu, Yi-Chuang Xu, Kostas Pantopoulos, Xiao-Ying Tan, Xiao-Lei Wei, Hua Zheng, Zhi Luo
Summary: High-carbohydrate diet and high glucose induce glycogen accumulation, inhibit glycophagy, and glucose production in liver tissues and hepatocytes. This inhibition is mediated by the OGT1-AKT1-FOXO1Ser238 pathway. These findings provide insights into potential intervention strategies for glycogen storage disorders.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Zhengyu Qian, Jingwei Ye, Jinteng Li, Yunshu Che, Wenhui Yu, Peitao Xu, Jiajie Lin, Feng Ye, Xiaojun Xu, Zepeng Su, Dateng Li, Zhongyu Xie, Yanfeng Wu, Huiyong Shen
Summary: This study aimed to explore the role of crotonylation in myogenic differentiation and its potential as a target in myogenic dysfunction diseases. Through in vitro and in vivo experiments, it was confirmed that crotonylation affects muscle differentiation and the crotonylation eraser HDAC3 regulates myogenic differentiation. The findings contribute to a better understanding of the pathophysiological processes of muscle injury repair and sarcopenia.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Reproductive Biology
Nannan Kang, Huizhi Shan, Junxia Wang, Jie Mei, Yue Jiang, Jidong Zhou, Chenyang Huang, Hui Zhang, Mei Zhang, Xin Zhen, Guijun Yan, Haixiang Sun
Summary: CAPN7 negatively regulates decidualization of human endometrial stromal cells by hydrolyzing AKT1 and promoting its phosphorylation. Increased expression of CAPN7 in endometriosis suggests that dysregulation of CAPN7 may be a potential cause of endometriosis.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Orarat Ginsawaeng, Carolin Heise, Rohit Sangwan, Daniel Karcher, Itzell Euridice Hernandez-Sanchez, Arun Sampathkumar, Ellen Zuther
Summary: LEA proteins play important roles in plant stress tolerance, mainly expressed in dry seeds. Research showed that LEA_4 proteins are localized in the endoplasmic reticulum, nucleus, mitochondria, and plastids, with some proteins forming condensates in the cytoplasm. The study also evaluated bioinformatic tools for predicting subcellular localization and promoter motifs of the LEA_4 proteins.
Article
Biochemistry & Molecular Biology
Fengwu Zhang, Yongchao Zhao, Yi Sun
Summary: USP2 was identified as a new deubiquitylase that stabilizes SKP2 protein by disrupting its interaction with substrates, thereby affecting cell growth regulation. Inactivation of USP2 can shorten SKP2 protein half-life and enhance its ubiquitylation and degradation, but surprisingly, USP2-stabilized SKP2 does not destabilize its substrates p21 and p27. Knockdown or inhibition of USP2 can partially mediate growth suppression through modulation of SKP2 and its substrates.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Shiho Hisaoka, Jin Osawa, Riku Kobashi, Atsuhiko Ishida, Isamu Kameshita, Noriyuki Sueyoshi
Summary: Research has shown that BMP2-inducible kinase (BMP2K) forms liquid-liquid phase separated droplets in the cytoplasm, regulated by a glutamine-rich area and hyperosmolarity. The cytoplasmic localization of BMP2K is managed through the inhibition of nuclear localization signal (NLS) function via phosphorylation of Ser-1010 and the antagonism of the NLS by a newly discovered cytoplasmic localization region. These findings provide a biochemical foundation for the advancement of BMP2K-related cell biology, structural biology, and pathophysiology.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Sevgican Demir, Gretchen Wolff, Annika Wieder, Adriano Maida, Lea Buehler, Maik Brune, Oksana Hautzinger, Annette Feuchtinger, Tanja Poth, Julia Szendroedi, Stephan Herzig, Bilgen Ekim Uestuenel
Summary: In this study, the researchers identified TSC22D4 as a novel partner for Akt1, a critical mediator in insulin signaling. The interaction between TSC22D4 and Akt1 affects insulin signaling and glucose metabolism, with the interaction being enhanced during energy deprivation and oxidative stress, while impaired during refeeding or exposure to glucose and insulin.
Article
Multidisciplinary Sciences
Samuel Hume, Claudia P. Grou, Pauline Lascaux, Vincenzo D'Angiolella, Arnaud J. Legrand, Kristijan Ramadan, Grigory L. Dianov
Summary: Efficient entry into S phase of the cell cycle is controlled by the NUCKS1-SKP2-p21/p27 axis. This pathway integrates mitogenic and DNA damage signals to regulate cell cycle progression, with potential implications in cancer development.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Pu Li, Tingting Chen, Peng Kuang, Fujun Liu, Zhongmin Li, Fangfang Liu, Yu Wang, Wenfeng Zhang, Xiuyu Cai
Summary: This study reveals that the Aurora-A/FOXO3A/SKP2 axis promotes tumor progression in ccRCC, and the double inhibition of SKP2 and Aur-A shows significant synergistic effect, indicating a potential new therapeutic strategy for ccRCC.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Mei Zhang, Xinyu Cai, Jingyu Liu, Jidong Zhou, Qingqing Shi, Yue Jiang, Nannan Kang, Xin Zhen, Min Wu, Panpan Qiu, Guijun Yan, Haixiang Sun, Dong Li
Summary: Decidualization is crucial for successful pregnancy, and abnormal decidualization is a major cause of recurrent implantation failure. This study found that the lncRNA lncSAMD11-1:1 is significantly decreased in the endometrium of patients with recurrent implantation failure. Overexpression of lncSAMD11-1:1 promotes decidualization and embryo implantation, improving decidualization in patients with recurrent implantation failure.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Cell Biology
Ganesh Kumar Barik, Osheen Sahay, Anindya Mukhopadhyay, Rajesh Kumar Manne, Sehbanul Islam, Anup Roy, Somsubhra Nath, Manas Kumar Santra
Summary: This study reveals that FBXW2 functions as a tumor suppressor and AKT kinase plays a key role in the oncogenic function of Moesin in breast cancer. AKT phosphorylates Moesin at a specific site, preventing its degradation by FBXW2 and thus contributing to the role of Moesin in breast cancer progression. Additionally, Moesin also acts as an upstream regulator of the oncogene SKP2, stabilizing it by preventing its degradation.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Xinfang Yu, Li Zhou, Wenbin Liu, Lijun Liu, Feng Gao, Wei Li, Haidan Liu
Summary: Skp2 is highly expressed in human colorectal cancer and plays a role in promoting tumorigenesis by stabilizing Mcl-1. Blocking Skp2 expression reduces tumorigenic properties of CRC cells, while Skp2 deficiency enhances sensitivity of CRC cells to irradiation treatments.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Martine Palma, Catherine Leroy, Sophie Salome-Desnoulez, Elisabeth Werkmeister, Rebekah Kong, Marc Mongy, Herve Le Hir, Fabrice Lejeune
Summary: Nonsense-mediated mRNA decay (NMD) is a highly regulated quality control mechanism that degrades mRNAs containing premature termination codons. AKT1 kinase plays a crucial role in NMD by phosphorylating UPF1, which may have implications in cancer processes and therapy due to its overactivation in cancer cells.
NUCLEIC ACIDS RESEARCH
(2021)
