Journal
CELL CYCLE
Volume 9, Issue 10, Pages 1986-1989Publisher
LANDES BIOSCIENCE
DOI: 10.4161/cc.9.10.11727
Keywords
APC/C; p27; Skp2; ubiquitin; Emi1; differentiation
Categories
Funding
- Israel Cancer Research Fund
- Israel Chief Scientist Office, Ministry of Health
Ask authors/readers for more resources
We have recently shown that Skp2 levels are high in undifferentiated human embryonic stem cells, but decline rapidly following induction of differentiation, thereby leading to accumulation of p27. Changes in Skp2 levels were found to be caused mainly by its rate of degradation. Here we show that the activity of APC/C-Cdh1, the ubiquitin ligase that targets Skp2 for degradation, increases markedly during the differentiation process of human embryonic stem cells. APC/C-Cdh1 is present but inactive in undifferentiated embryonic stem cells and becomes active in the differentiated state. The rise in APC/C-Cdh1 activity with differentiation appears to be due, at least in part, to a dramatic decline in the levels of its inhibitor Emi1. In addition, protein kinase activity also appears to contribute to the suppression of APC/C-Cdh1 activity in undifferentiated stem cells, possibly by inhibitory phosphorylation of Cdh1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available