Article
Biochemical Research Methods
Souvik Seal, Debashis Ghosh
Summary: This study presents a new method for analyzing the interaction of proteins or markers in the tumor microenvironment of cancer patients, finding co-expression to be associated with disease risk and survival, and demonstrating the robustness of the method.
Article
Cell Biology
Renjie Liao, Manas Mondal, Christopher D. Nazaroff, Diego Mastroeni, Paul D. Coleman, Joshua Labaer, Jia Guo
Summary: The method presented achieves highly sensitive and multiplexed protein analysis using cleavable fluorescent tyramide and off-the-shelf antibodies, enhancing detection sensitivity and reducing imaging time. By studying genetically identical cell populations and profiling neurons in human brain tissue, it reveals protein expression heterogeneity and the presence of distinct neuron clusters within different sub-regions of the hippocampus.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Thai Pham, Renjie Liao, Joshua Labaer, Jia Guo
Summary: This study introduces a highly sensitive and accurate protein in situ profiling approach using off-the-shelf antibodies and cleavable fluorescent tyramide, enabling multiplexed protein quantification in single cells.
Article
Immunology
Yavuz Selim Saglam, Serkan Yildirim, Mustafa Ozkaraca, Serdar Altun
Summary: In this study, the presence of Leptospira interrogans antigens in naturally infected bovine kidney samples was determined using immunohistochemical and immunofluorescence methods. The antigens were detected in 7.14% of the samples tested by both methods. Despite lower rates compared to previous studies, it is important to recognize that this issue still poses problems for animal health.
MICROBIAL PATHOGENESIS
(2022)
Article
Oncology
Ramona Erber, Sareetha Kailayangiri, Hanna Huebner, Matthias Ruebner, Arndt Hartmann, Lothar Haeberle, Julia Meyer, Simon Voelkl, Andreas Mackensen, Laura Landgraf, Carol Geppert, Ruediger Schulz-Wendtland, Matthias W. Beckmann, Peter A. Fasching, Nicole Farwick, Claudia Rossig, Paul Gass
Summary: The study found that GD2 is expressed in more than 50% of breast cancer cases, with the highest frequency in hormone receptor-positive tumors. However, GD2 expression was not significantly associated with patient outcome.
Article
Multidisciplinary Sciences
Harim Oh, Chul Hwan Kim, Yoo Jin Lee
Summary: The study evaluated the diagnostic value of immunohistochemical staining for bullous pemphigoid (BP) detection, showing that IHC staining has high sensitivity and specificity, being helpful for the diagnosis of DIF-confirmed BP cases and clinically suspected BP cases.
SCIENTIFIC REPORTS
(2022)
Review
Immunology
Luciano Sturmer de Fraga, Isadora D'avila Tassinari, Jeferson Jantsch, Renata Padilha Guedes, Victorio Bambini-Junior
Summary: Microscopy techniques have advanced significantly, allowing imaging in live cells and animals to study neuroinflammation. Various markers and methodologies, such as immunohistochemistry and two-photon microscopy, are used to unravel the mechanisms of neuroinflammation in distinct conditions. Integration of omics with microscopy is developing and will enhance understanding of molecular mechanisms in neuroinflammation.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Shi-Wei Zhang, Wei Chen, Xiao-fang Lu, Zhang Wen, Lei Hu, Yu-Hong Liu, Zheng Yang, Ling Xue, Qiao Su, Le-Ping Yan, Joaquim M. Oliveira, Rui L. Reis, Yu-Long He, Chang-Hua Zhang
Summary: This study introduces a user-friendly, cost-effective method for making FFPE and OCT embedded organoid blocks, facilitating histological analysis for researchers with no specialized equipment required.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Medicine, General & Internal
Yumiko Yamamoto, Yoshihiro Hayashi, Hideyuki Sakaki, Ichiro Murakami
Summary: For melanoma treatment, early diagnosis and complete resection of the primary tumor are crucial, and the detection of factors related to metastasis is also essential. This study investigated 30 Japanese patients with Stage I or II melanoma to identify factors statistically associated with metastasis. The analysis revealed that older age at onset and positive immunohistochemical expressions of Nestin and Fascin were significantly related to metastasis. Double immunofluorescence staining was performed to observe Nestin and Fascin expression at different stages. This is the first study to report the presence of Nestin and Fascin double-positive melanoma cells.
Article
Veterinary Sciences
Luke M. Jones, Phillippa C. Hawes, Francisco J. Salguero, Javier Castillo-Olivares
Summary: African Horse Sickness (AHS) is a vector-borne viral disease that can have a high mortality rate in non-immune equine populations. The pathogenesis of this disease in horses is not fully understood, but small animal models, such as IFNAR(-/-) mice, have been used to study its pathology. In this study, AHSV-4 infection in IFNAR(-/-) mice resulted in lesions in various organs, including necrosis in the spleen, inflammatory infiltration in the liver and brain, and pneumonia. The IFNAR(-/-) mouse model proved valuable for studying the immuno-biology of AHSV infections and evaluating candidate vaccines.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Immunology
Songbai Yang, Huaijin Liu, Zhenyu Chen, Han Wang, Xiangchen Li, Xiaolong Zhou, Ayong Zhao
Summary: This study investigates how Japanese encephalitis virus (JEV) evades immunity by disrupting the structure of PML-nuclear bodies (PML-NBs) through interaction with PML isoforms, potentially attenuating the host's antiviral immune response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Oncology
Simon Gray, Christian H. Ottensmeier
Summary: NSCLC remains a significant cause of morbidity and mortality, and the effectiveness of ICIs treatment is limited for most patients. The use of MAB technologies to analyze TME can provide insights into treatment susceptibility, resistance, and prognosis factors, and discover new therapeutic approaches.
Article
Microbiology
Carina Strohmayer, Andrea Klang, Stefan Kummer, Ingrid Walter, Christoph Jindra, Christiane Weissenbacher-Lang, Torben Redmer, Sibylle Kneissl, Sabine Brandt
Summary: This study found that (partial) epithelial-mesenchymal transition events occur in equine HNSCCs, suggesting CD44 and CD271 as potential malignancy markers.
Article
Oncology
Ruopeng Su, Lei Chen, Zhou Jiang, Minghao Yu, Weiwei Zhang, Zehua Ma, Yiyi Ji, Kai Shen, Zhixiang Xin, Jun Qi, Wei Xue, Qi Wang
Summary: In this study, the researchers investigated the status of androgen receptor (AR) in prostate cancer with neuroendocrine differentiation. They found the existence of AR(HIGH)/NEHIGH prostate cancer and observed a correlation between AR loss tumors and adverse clinical stages. The researchers also observed the co-localization of AR and NE markers in prostate cancer cells using immunofluorescence staining. This study provides new insights into the cellular plasticity of prostate cancer with neuroendocrine differentiation and has implications for therapeutic development.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alessandro Gambella, Chiara Pitino, Antonella Barreca, Alberto Nocifora, Manuela Maria Giarin, Luca Bertero, Luigi Biancone, Dario Roccatello, Mauro Papotti, Paola Cassoni
Summary: DNAJB9 as a diagnostic marker of FGN has high specificity and sensitivity, and its clinical application can greatly improve the efficiency and accuracy of FGN diagnosis.
