Article
Plant Sciences
Jing Xu, Chaofan Fu, Tao Li, Xiaoyan Xia, Huixing Zhang, Xude Wang, Yuqing Zhao
Summary: The study found that AE inhibited apoptosis and increased antioxidant levels in STZ-induced MIN6 cells. In the diabetic rat model, the AE group showed lower blood glucose levels, increased insulin secretion, and alleviated weight loss.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Review
Microbiology
Elizabeth A. Rucks
Summary: Type III secretion systems (T3SS) are utilized by Gram-negative pathogens to deliver effector proteins into target eukaryotic cells for manipulating cell functions and enhancing pathogenesis. This review will discuss the history, biochemical characterization, and function of chlamydial T3SS, as well as the use of heterologous/surrogate models for studying it. The review will also cover the history of chlamydial effectors and recent advances in the field.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Brianna Steiert, Carolina M. Icardi, Robert Faris, Paige N. McCaslin, Parker Smith, Aloysius J. Klingelhutz, Peter M. Yau, Mary M. Weber
Summary: The centrosome is crucial for mitotic spindle assembly, chromosome segregation, and cell division. Infection by Chlamydia trachomatis (C.t.) leads to blocked cytokinesis, supernumerary centrosomes, and multipolar spindles, but the mechanisms behind these cellular abnormalities remain largely unknown. This study shows that the secreted effector protein CteG binds to CETN2 and is necessary for infection-induced centrosome amplification. These findings provide insights into C.t.-induced cellular abnormalities and its potential contribution to cancer risk.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Endocrinology & Metabolism
Jonatan Bagger, Magnus F. G. Grondahl, Asger Lund, Jens J. Holst, Tina Vilsboll, Filip K. Knop
Summary: The study showed that GLP-1 potentiated the glucagon-suppressive effect of glucose in a dose-dependent manner in healthy individuals and patients with type 2 diabetes. However, patients with type 1 diabetes were insensitive to the glucagonostatic effects of both glucose and GLP-1.
Article
Immunology
Burcak Yesildag, Joan Mir-Coll, Aparna Neelakandhan, Claire B. Gibson, Nikole R. Perdue, Chantal Rufer, Maria Karsai, Adelinn Biernath, Felix Forschler, Patricia Wu Jin, Patrick M. Misun, Alexandra Title, Andreas Hierlemann, Frederik F. Kreiner, Johnna D. Wesley, Matthias G. von Herrath
Summary: In this study, three in-vitro islet-immune injury models were established to investigate the immunopathogenesis of type 1 diabetes. The results showed that liraglutide protected pancreatic cells from immune attack.
CLINICAL IMMUNOLOGY
(2022)
Article
Microbiology
Jing Wang, Yuan Luo, Yilin Gu, Hai-Lei Wei
Summary: Pseudomonas fluorescens 2P24 is a plant growth-promoting rhizobacterium with a complete SPI-1 type III secretion system gene cluster. HilA and InvF were identified as two transcriptional regulator homologs within the SPI-1 T3SS gene cluster, regulating gene expression positively and up-regulating genes, respectively. The SPI-1 mutants of 2P24 showed different phenotypes in resistance against Fusarium graminearum, biofilm formation, and chemotactic responses to glucose compared to the wild type strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Ce Zhang, Min Liu, Ying Wu, Xixi Li, Chen Zhang, Douglas R. Call, Ming Liu, Zhe Zhao
Summary: In Vibrio parahaemolyticus, the expression of T3SS1 is regulated by a complex quorum sensing phosphorelay circuit involving the hybrid histidine kinase ArcB, sensor kinase LuxQ, and response regulator LuxO. The study shows that LuxQ can directly interact with ArcB under high oxygen conditions, leading to phosphorylation of LuxO and activation of T3SS1 expression. Under hypoxic conditions, ArcB autophosphorylates and phosphorylates ArcA, but ArcA does not participate in regulating T3SS1 expression.
Article
Veterinary Sciences
Xinxin Gao, Xu Zhang, Liqiang Jiang, Jingnan Xu, Wei Liu, Yuxiao Qian, Yuqian Jiang, Qinqin Jin, Hongrong Hong, Meiyi Chen, Zha Jin, Zhengkai Wei, Zhengtao Yang, Haoji Zhang
Summary: This study found that Forsythin (FOR) has antioxidant effects on 8-hydroxybutyrate (8-HB) stimulated bovine macrophages (BMs) and can reduce the expression of inflammatory genes through regulating multiple signaling pathways. FOR may be a potential medicine to alleviate 8-HB-induced inflammatory response.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Lars Krogvold, Ida Maria Mynarek, Erica Ponzi, Freja Barrett Mork, Trine Witzner Hessel, Trine Roald, Nina Lindblom, Jacob Westman, Peter Barker, Heikki Hyoety, Johnny Ludvigsson, Kristian F. Hanssen, Jesper Johannesen, Knut Dahl-Jorgensen
Summary: The results suggest that antiviral treatment may help preserve insulin production in children and adolescents with new-onset type 1 diabetes.
Review
Cell Biology
Matilde Masini, Mara Suleiman, Michela Novelli, Lorella Marselli, Piero Marchetti, Vincenzo De Tata
Summary: Mast cells are key regulators of the immune system, involved in both health mechanisms and disease pathogenesis. In diabetes, increased mast cell presence in the pancreas due to autoimmune destruction of insulin-secreting beta cells could offer new therapeutic targets.
Article
Oncology
Anlan Hong, Meng Cao, Dongqing Li, Yixin Wang, Guoqiang Zhang, Fang Fang, Liang Zhao, Qiang Wang, Tong Lin, Yan Wang
Summary: The study revealed that lnc-PKNOX1-1 is significantly downregulated in melanoma and associated with its pathological type and Breslow thickness. Upregulation of lnc-PKNOX1-1 inhibits the malignant phenotype of melanoma by suppressing IL-8 expression.
Article
Multidisciplinary Sciences
Stephan Wimmi, Alexander Balinovic, Hannah Jeckel, Lisa Selinger, Dimitrios Lampaki, Emma Eisemann, Ina Meuskens, Dirk Linke, Knut Drescher, Ulrike Endesfelder, Andreas Diepold
Summary: Current research shows that in gastrointestinal pathogens, the external pH plays a crucial role in regulating the assembly of T3SS cytosolic components, preventing T3SS activity in the stomach and facilitating its reactivation in the intestine.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Qi Yan, Linxin Pan, Shunli Qi, Fang Liu, Zhen Wang, Cheng Qian, Lijian Chen, Jian Du
Summary: The up-regulation of RNF2 in liver fibrosis enhances HSC activation and exerts fibrogenic effects by inhibiting the ERK/p38 signaling pathway induced by TGF-beta 1. The inhibition of RNF2 may be a promising therapeutic target for liver fibrosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yasuo Okamoto, Keisuke Kitakaze, Yasuhiro Takenouchi, Shinya Yamamoto, Hironobu Ishimaru, Kazuhito Tsuboi
Summary: The study highlights the crucial role of S1P(2) in the activation of STAT6 in macrophages, with its signaling pathway involving the Rho-Rho kinase pathway. Knockout of S1P(2) weakened the IL-4/IL-13-induced phosphorylation of STAT6 and mRNA expression of the M2 marker ARG1.
CELLULAR SIGNALLING
(2021)
Article
Biochemistry & Molecular Biology
Martin J. Ronis, Kelly E. Mercer, Kartik Shankar, Casey Pulliam, Kim Pedersen, Magnus Ingelman-Sundberg, Simonetta Friso, Derrick Samuelson, Luis Del Valle, Chris Taylor, David A. Welsh
CHEMICO-BIOLOGICAL INTERACTIONS
(2020)
Article
Substance Abuse
Kristina L. Bailey, Derrick R. Samuelson, Todd A. Wyatt
Summary: Alcohol misuse has long been recognized as a factor in the pathophysiology of lung diseases, affecting susceptibility to infections and lung barrier function. This suggests that alcohol-related lung issues could contribute to both the susceptibility and severity of COVID-19 infections.
Article
Biodiversity Conservation
Mystera M. Samuelson, Eric E. Pulis, Candis Ray, Covadonga R. Arias, Derrick R. Samuelson, Erin E. Mattson, Moby Solangi
ENDANGERED SPECIES RESEARCH
(2020)
Article
Substance Abuse
Min Gu, Derrick R. Samuelson, Christopher M. Taylor, Patricia E. Molina, Meng Luo, Robert W. Siggins, Judd E. Shellito, David A. Welsh
Summary: Chronic alcohol consumption leads to a decrease in MAIT cells in barrier tissues, while CD69 expression on tissue-associated MAIT cells is increased in alcohol-fed (AF) mice. Th1 cytokines show tissue-specific expression changes in AF animals. Transplantation of fecal microbiota from AF mice results in a MAIT cell profile aligned to that of AF mouse donor, and antibiotic treatment abolishes the differences in MAIT cells between AF and pair-fed (PF) animals.
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Nicholas M. Negretti, Christopher R. Gourley, Prabhat K. Talukdar, Geremy Clair, Courtney M. Klappenbach, Cody J. Lauritsen, Joshua N. Adkins, Michael E. Konkel
Summary: Campylobacter jejuni invades intestinal cells by secreting protein effectors into the host cell cytosol via the flagellum. One of these effectors, CiaD, binds to host protein IQGAP1, leading to increased activity of small GTPase Rac1, which modulates actin reorganization and bacterial internalization.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Luyao Ma, Michael E. Konkel, Xiaonan Lu
Summary: This study found that C. jejuni biofilms significantly enhanced HGT, increasing the frequency up to 173-fold compared to planktonic cells. HGT in biofilms was not associated with biofilm biomass, cell density, or bacterial metabolic activity, but was correlated with extracellular DNA amount. The presence of synergistic HGT effect between different strains of C. jejuni was also observed.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Immunology
Sannette C. Hall, Deandra R. Smith, Shetty Ravi Dyavar, Todd A. Wyatt, Derrick R. Samuelson, Kristina L. Bailey, Daren L. Knoell
Summary: Zinc is essential for immune function and host defense, regulated by zinc transporters. Zinc deficiency can increase susceptibility to bacterial infections, especially Streptococcus pneumoniae. Myeloid cells, such as macrophages and dendritic cells, play a critical role in shaping the immune response in the lung against bacterial pathogens.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Microbiology
Courtney M. Klappenbach, Nicholas M. Negretti, Jesse Aaron, Teng-Leong Chew, Michael E. Konkel
Summary: Our study reveals that C. jejuni infection alters the size and topology of focal adhesions in epithelial cells, leading to changes in cell adhesion strength and motility. The increased phosphorylation of paxillin and altered turnover at focal adhesions may contribute to the effects on cell behavior. Additionally, inhibiting protein synthesis or using specific mutants of C. jejuni can partially reverse these effects, suggesting a potential mechanism for the observed restricted intestinal repair in infected animals.
Article
Biology
Derrick R. Samuelson, Min Gu, Judd E. Shellito, Patricia E. Molina, Christopher M. Taylor, Meng Luo, David A. Welsh
Summary: Alcohol impairs immune function and cellular transport, increasing susceptibility to bacterial pneumonia. Microbial metabolites can mitigate alcohol-induced risks, partially through AhR. Microbiota therapeutics are effective in reducing the risk of alcohol-associated bacterial pneumonia.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Derrick R. Samuelson, Deandra R. Smith, Kelly C. Cunningham, Todd A. Wyatt, Sannette C. Hall, Daryl J. Murry, Yashpal S. Chhonker, Daren L. Knoell
Summary: Pneumococcal pneumonia is a major cause of disease and death worldwide, with compromised immune function and an insufficient dietary intake of zinc increasing the risk. This study reveals the critical role of the zinc transporter ZIP8 in host defense and the adverse impact of a common defective ZIP8 allele on the gut microbiota essential for lung immunity. Loss of ZIP8 function leads to intestinal dysbiosis and impaired host defense in the lung, highlighting the need for further investigation and improved surveillance and treatment strategies given the high incidence of dietary zinc deficiency and the ZIP8 variant allele in the human population.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Vineet Joshi, Yashpal S. Chhonker, Dhruvkumar Soni, Kelly C. Cunningham, Derrick R. Samuelson, Daryl J. Murry
Summary: A novel LC-MS/MS method was developed to quantify indole concentrations in mouse plasma and tissues, which could serve as a biomarker for several diseases. This method separated and measured the ion state of indole and an internal standard, allowing for accurate determination of indole concentrations range in different samples. This study contributes to the understanding of the role of indole as a biomarker and its implications in various disease states.
Review
Substance Abuse
Kathryn Crotty, Paige Anton, Leon G. Coleman, Niya L. Morris, Sloan A. Lewis, Derrick R. Samuelson, Rachel H. McMahan, Phillipp Hartmann, Adam Kim, Anuradha Ratna, Pranoti Mandrekar, Todd A. Wyatt, Mashkoor A. Choudhry, Elizabeth J. Kovacs, Rebecca McCullough, Samantha M. Yeligar
Summary: This critical review describes the potential mechanisms by which alcohol misuse leads to immune system dysfunction and highlights the current research gaps.
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Amber D. Gabbert, Jennifer L. Mydosh, Prabhat K. Talukdar, Lisa M. Gloss, Jason E. McDermott, Kerry K. Cooper, Geremy C. Clair, Michael E. Konkel
Summary: Campylobacter jejuni is the most common bacterial cause of gastroenteritis worldwide, causing millions of cases of inflammatory diarrhea. It is associated with post-infection complications in developed countries and malnutrition and growth-stunting in low- and middle-income countries. Our understanding of C. jejuni pathogenesis is incomplete, but we focus on the secretion systems and their role in host-cell interactions and disease establishment. The flagellar type III secretion system and putative effectors are of particular interest.
Article
Microbiology
Derrick R. Samuelson, Deandra R. Smith, Kelly C. Cunningham, Sabah Haq, Daniel N. Villageliu, Christi M. Ellis, Niaz Bahar Chowdhury, Amanda E. Ramer-Tait, Jeffrey D. Price, Daren L. Knoell
Summary: Intestinal dysbiosis increases susceptibility to infection due to metabolic profile changes, resulting in increased morbidity. The study found that ZIP8, a type of zinc transporter, is important for myeloid cells in maintaining host defense against bacterial pneumonia. Furthermore, a common variant of ZIP8 is closely associated with inflammation-based disorders and bacterial infection. The researchers developed a new model to study the effects of ZIP8-mediated intestinal dysbiosis on pulmonary host defense, independent of genetic factors. The results showed that the intestinal microbiota, regardless of host genetics, play a crucial role in regulating host defense in the lung against infection. These findings support future microbiome-based intervention studies, considering the high prevalence of zinc deficiency and the rs13107325 allele in humans.
Article
Cell Biology
Kelly C. Cunningham, Deandra R. Smith, Daniel N. Villageliu, Christi M. Ellis, Amanda E. Ramer-Tait, Jeffrey D. Price, Todd A. Wyatt, Daren L. Knoell, Mystera M. Samuelson, Patricia E. Molina, David A. Welsh, Derrick R. Samuelson
Summary: Chronic alcohol abuse leads to alterations in the gastrointestinal microbiota, resulting in behavior changes, physiological alterations, and immunological effects. This study developed a humanized alcohol-microbiota mouse model and found that colonization of mice with fecal microbiota from individuals with high AUDIT scores increased susceptibility to pneumonia.