Journal
CELL COMMUNICATION AND ADHESION
Volume 17, Issue 2, Pages 23-33Publisher
TAYLOR & FRANCIS INC
DOI: 10.3109/15419061.2010.487956
Keywords
Cx43; detergent micelles; NMR; TFE
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Funding
- United States Public Health Service [GM072631, HL039707]
- Nebraska Research Initiative
- Eppley Cancer Center [P30CA036727]
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pH-mediated gating of Cx43 channels following an ischemic event is believed to contribute to the development of lethal cardiac arrhythmias. Studies using a soluble version of the Cx43 carboxyl-terminal domain (Cx43CT; S255-I382) have established the central role it plays in channel regulation; however, research in the authors' laboratory suggests that this construct may not be the ideal model system. Therefore, we have developed a more 'native-like' construct (Cx43CT attached to the 4th transmembrane domain [TM4-Cx43CT; G178-I382]) than the soluble Cx43CT to further investigate the mechanism(s) governing this regulation. Here, we utilize circular dichroism and nuclear magnetic resonance (NMR) were used to validate the TM4-Cx43CT for studying channel gating and optimize solution conditions for structural studies. The data indicate that, unlike the soluble Cx43CT, the TM4-Cx43CT is structurally responsive to changes in pH, suggesting the presence of the TM4 facilitates pH-induced structural alterations. Additionally, the optimal solution conditions for solving the NMR solution structure include 10% 2,2,2 trifluoroethanol and removal of the 2(nd) extracellular loop (G178-V196).
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