Journal
CELL CALCIUM
Volume 51, Issue 2, Pages 117-130Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2011.11.010
Keywords
Transcription regulation; Cryptogein; Calcium signaling; Nicotiana tabacum (tobacco BY-2)
Categories
Funding
- Association pour la Recherche sur les Nicotianae
- Agence Nationale pour la Recherche [ANR-06-BLAN-370]
- Centre National de la Recherche Scientifique
- Universite de Toulouse III (UPS)
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Cryptogein is a proteinaceous elicitor secreted by the oomycete Phytophthora cryptogea, which induces a hypersensitive response in tobacco plants. We have previously reported that in tobacco BY-2 cells treated with cryptogein, most of the genes of the phenylpropanoid pathway were upregulated and cell wall-bound phenolics accumulated. Both events were Ca2+ dependent. In this study, we designed a microarray covering a large proportion of the tobacco genome and monitored gene expression in cryptogein-elicited BY-2 cells to get a more complete view of the transcriptome changes and to assess their Ca2+ dependence. The predominant functional gene categories affected by cryptogein included stress- and disease-related proteins, phenylpropanoid pathway, signaling components, transcription factors and cell wall reinforcement. Among the 3819 unigenes whose expression changed more than fourfold, 90% were Ca2+ dependent, as determined by their sensitivity to lanthanum chloride. The most Ca2+-dependent transcripts upregulated by cryptogein were involved in defense responses or the oxylipin pathway. This genome-wide study strongly supports the importance of Ca2+-dependent transcriptional regulation of regulatory and defense-related genes contributing to cryptogein responses in tobacco. (C) 2011 Elsevier Ltd. All rights reserved.
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