Review
Cell Biology
Yanina Bogliotti, Mark Vander Roest, Aras N. Mattis, Robert G. Gish, Gary Peltz, Robin Anwyl, Salah Kivlighn, Eric R. Schuur
Summary: Liver disease is a major cause of global mortality, with late-stage liver disease being characterized by hepatocyte injury, inflammation, and aberrant tissue healing. Treatment options for liver disease are limited, but cell replacement therapy using hepatocyte-like cells shows promise as a safe and effective treatment option.
Article
Gastroenterology & Hepatology
Mauricio Lisker-Melman, Abdus S. Wahed, Marc G. Ghany, Raymond T. Chung, Wendy C. King, David E. Kleiner, Atul K. Bhan, Mandana Khalili, Mamta K. Jain, Mark Sulkowski, David K. Wong, Gavin Cloherty, Richard K. Sterling
Summary: In patients coinfected with HBV and HIV, HBV RNA and HBcrAg can still be detected despite viral suppression, and their levels are associated with the extent of HBsAg and HBcAg staining in liver cells. This suggests that residual transcription and translation may contribute to liver pathology even when viral replication is suppressed.
Article
Cell Biology
Jeniffer Farias Dos Santos, Bruna Leticia Freitas-Marchi, Gustavo Roncoli Reigado, Silvia Romano de Assis, Silvya Stuchi Maria Engler, Felipe Santiago Chambergo Alcalde, Viviane Abreu Nunes
Summary: In skin traumas, tissue engineering using mesenchymal stem cells (MSC) from the umbilical cord shows promise for skin regeneration. MSCs can differentiate into keratinocytes and express important epidermal markers, indicating their potential use in producing skin substitutes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Arshi Khanam, Lydia S. Y. Tang, Shyam Kottilil
Summary: CD8(+)CXCR5(+) cells in chronic hepatitis B infection exhibit effector phenotypes, produce HBV-specific cytokines despite increased PD-1 expression, and are associated with reduction in HBsAg and HBV-DNA, indicating their potential therapeutic significance as they efficiently support B-cell function.
Article
Cell & Tissue Engineering
Wenda Xi, Wendong Chen, Weihong Sun, Xiangxiao Li, Zhimin Suo, Gonghao Jiang, Pingjin Gao, Qun Li
Summary: This study found that PDGFR alpha(+) S-MSCs can differentiate into BAs and this process is regulated by mitochondrial activity. The study also showed that BAT directly plays a role in alleviating Ang II-induced hypertension. Further investigation into the therapeutic potential of BAT for preventing hypertension-induced organ remodeling is warranted.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Yansiwei Cheng, Yuhao Zhou, Wenjun Lv, Qing Luo, Guanbin Song
Summary: Research shows that microgravity inhibits dermal fibroblastic differentiation of BMSCs by suppressing ERK/beta-catenin signaling, indicating a potential intervention target for repairing skin injuries in microgravity conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Eleanor Luce, Clara Steichen, Soumeya Abed, Anne Weber, Philippe Leboulch, Leila Maouche-Chretien, Anne Dubart-Kupperschmitt
Summary: The study demonstrates that siPSCs can differentiate into liver cells and self-organize into 3D structures, facilitating research on liver development and the development of co-culture systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Lucas Eduardo Botelho de Souza, Fernanda Ursoli Ferreira, Carolina Hassibe Thome, Heloisa Brand, Maristela Delgado Orellana, Vitor Marcel Faca, Aparecida Maria Fontes, Dimas Tadeu Covas
Summary: Melanoma cells have the capability to activate an EMT-like program and acquire metastatic traits through distinct pathways induced by MSCs' secretome, both in mouse and human cells.
Article
Agriculture, Dairy & Animal Science
Young-Bum Son, Yeon Ik Jeong, Yeon Woo Jeong, Mohammad Shamim Hossein, Alex Tinson, Kuhad Kuldip Singh, Woo Suk Hwang
Summary: This study evaluated the biological characteristics of mesenchymal stem cells (MSCs) derived from skeletal muscle, dermal skin, and adipose tissue from a single donor Camelus dromedarius, demonstrating differences in proliferation and differentiation abilities. The adipogenic differentiation capacity of adipose tissue-derived MSCs was significantly higher, while the osteoblast differentiation capacity was lower compared to skeletal muscle and dermal skin-derived MSCs. Further comparative analysis revealed variations in pluripotent marker expression and alkaline phosphatase activity among the different tissue-derived MSCs.
Article
Immunology
Minmin Liu, Yarong Song, Yi Li, Xingwen Yang, Hui Zhuang, Jie Li, Jie Wang
Summary: Mother-to-child transmission (MTCT) is the main route of hepatitis B virus (HBV) infection, but the virological factors affecting HBV MTCT are not fully understood. This study found that among mother-infant pairs with positive maternal hepatitis B surface antigen (HBsAg), the average nucleotide mutation rate in the HBV preS1 promoter (SPI) region was significantly higher in the immunoprophylaxis success group than in the immunoprophylaxis failure group. The C2729T mutation in the HBV SPI region was the most frequent mutation and it was found to reduce HBV production by suppressing the expression of large hepatitis B surface antigen (LHBs), indicating its potential role in the success of immunoprophylaxis for HBV MTCT.
Article
Multidisciplinary Sciences
Veronica Gatti, Claudia Fierro, Mirco Compagnone, Veronica La Banca, Alessandro Mauriello, Manuela Montanaro, Stefano Scalera, Francesca De Nicola, Eleonora Candi, Francesco Ricci, Luca Fania, Gerry Melino, Angelo Peschiaroli
Summary: Delta Np63, a master regulator of epithelial biology, interacts with Senataxin (SETX) to sustain the expression of epidermal differentiation genes and regulate the differentiation of keratinocytes. SETX physically binds the p63 DNA-binding motifs present in early epidermal differentiation genes KRT1 and ZNF750, facilitating R-loop removal and promoting gene expression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Medicine, Research & Experimental
Arezou Khosrojerdi, Sara Soudi, Ahmad Zavaran Hosseini, Sahar Ghaffari Khaligh, Seyed Mahmoud Hashemi
Summary: This study investigated the effect of different treatments on systemic and local inflammation in a mouse model of sepsis. MSC-derived exosomes combined with imipenem controlled inflammation and increased TCD4+ and Treg cell populations. Hepatocyte-derived exosomes combined with imipenem reduced liver inflammation and increased TCD8+ and Treg cell populations. The mixture of exosomes combined with imipenem showed the best function in reducing inflammation, maintaining all T lymphocyte populations, reducing liver damage, and increasing survival rate.
Article
Oncology
Shraddha Subramanian, Alexes C. Daquinag, Solmaz AghaAmiri, Sukhen C. Ghosh, Ali Azhdarinia, Mikhail G. Kolonin
Summary: Metastasis is a major cause of cancer-related deaths, and current chemoresistance makes metastatic cancers difficult to treat. This study identifies peptides that specifically target metastatic cells, demonstrating their potential as noninvasive imaging probes and vehicles for cytotoxic therapy delivery in preclinical cancer models.
Article
Microbiology
Hye Won Lee, Yongwook Choi, Ah Ram Lee, Cheol-Hee Yoon, Kyun-Hwan Kim, Byeong-Sun Choi, Yong Kwang Park
Summary: This study found that HGF-induced ACK1 inhibits HBV replication at the transcriptional level through activation of the MAPK-HNF signaling pathway, leading to suppression of viral gene expression and transcription, as well as decreased viral enhancer activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Yarong Song, Ying Lu, Yi Li, Minmin Liu, Hui Zhuang, Jie Li, Jie Wang
Summary: The x128-133del mutation is associated with successful immunoprophylaxis in infants born to hepatitis B e antigen (HBeAg)-positive mothers, as it weakens HBV replication by reducing the level of the HBx protein and attenuating the binding capacity of HNF4 alpha to HBV.
Article
Biochemistry & Molecular Biology
Chia-Rui Shen, Ya-Shan Chen, Yih-Shiou Hwang, Hsi-Jien Chen, Chao-Lin Liu
Summary: The study demonstrated that the translation efficiency mediated by IRES is lower when the target gene is downstream, leading to reduced protein production. Additionally, a significantly decreased level of protein production was observed when an upstream cistron was present.
BIOMEDICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Chiao-Fan Chiu, Jinn-Hsuan Ho, Eskedar Tessema, Yijing Lu, Chia-Rui Shen, Chang-Wei Lin, Norman Lu
Summary: Homogeneous catalysts with short fluorous chains were synthesized and used in the catalysis of alkynes' hydrosilylation. The thermomorphic mode was effectively used for catalyst recovery. The reduced fluorine content in the new catalyst is considered an important discovery in fluorous technology field.
Article
Virology
Yi-Jung Li, Hsin-Hsu Wu, Cheng-Hsu Chen, Hsu-Han Wang, Yang-Jen Chiang, Hsiang-Hao Hsu, See-Tong Pang, Robert Y. L. Wang, Ya-Chung Tian
Summary: The study demonstrates that BKPyVAN patients have increased risk of allograft losses, higher incidence of UTC, lower cancer-free survival rate, and earlier onset of UTC compared to non-BKPyVAN patients.
Article
Immunology
Sheng-Min Lo, Yih-Shiou Hwang, Chao-Lin Liu, Chia-Ning Shen, Wei-Hsin Hong, Wei-Cheng Yang, Meng-Hua Lee, Chia-Rui Shen
Summary: This study investigates the role of Toll-like receptor 7 (TLR7) in the development of experimental autoimmune uveitis (EAU). The results show that TLR7 agonist R848 exacerbates EAU progression and impairs the barrier function of retinal pigment epithelial (RPE) cells. The inhibition of TLR7 using shRNA effectively reduces disease severity. These findings highlight the immunomodulatory role of RPE TLR7 and provide a potential therapeutic strategy for autoimmune uveitis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jyuhn-Huarng Juang, Jiun-Jie Wang, Chia-Rui Shen, Sung-Han Lin, Chen-Yi Chen, Chen-Wei Kao, Chen-Ling Chen, Shu-Ting Wu, Zei-Tsan Tsai, Yun-Ming Wang
Summary: The study shows that manganese magnetism-engineered iron oxide nanoparticles (MnMEIO NPs) conjugated with exendin-4 (Ex4) can act as a contrast agent for directly tracing implanted mouse islet beta-cells using magnetic resonance imaging (MRI). This technology can also be used to track implanted porcine neonatal pancreatic cell clusters (NPCCs) containing ducts, endocrine, and exocrine cells. The MnMEIO-Ex4 NPs are excellent contrast agents for detecting and long-term monitoring implanted NPCCs by MRI.
Article
Chemistry, Organic
Eskedar Tessema, Yao-Wen Fan, Chiao-Fan Chiu, Vijayanath Elakkat, Hening Asti Rahayu, Chia-Rui Shen, Karthik Chimatahalli Shanthakumar, Peng Zhang, Norman Lu
Summary: In this study, a low fluorine content Pd complex was synthesized and demonstrated to exhibit good catalytic activity and recyclability in Suzuki-Miyaura and Sonogashira coupling reactions. It was also found that electron withdrawing groups on the aryl iodide can accelerate the reaction rate.
Article
Chemistry, Analytical
Chia-Hsuan Cheng, Yu-Chi Peng, Shu-Min Lin, Hiromi Yatsuda, Szu-Heng Liu, Shih-Jen Liu, Chen-Yen Kuo, Robert Y. L. Wang
Summary: Vaccination is a necessary measure to combat the COVID-19 pandemic, but monitoring antibody levels prior to vaccination is important to reduce unnecessary vaccinations. A novel SH-SAW biosensor can quickly and accurately quantify antibody levels. The study found that antibody concentrations significantly increased several days to weeks after vaccination, with mRNA vaccines achieving higher antibody concentrations.
Article
Education & Educational Research
Ding-Ping Chen, Su-Wei Chang, Annette Burgess, Brian Tang, Kuo-Chien Tsao, Chia-Rui Shen, Pi-Yueh Chang
Summary: This study aimed to explore potential factors affecting learning effectiveness in medical technology majors. The results showed that extracurricular studies and willingness to cooperate were positively associated with learning effectiveness, while weakened motivation due to uncertainty was negatively associated. It is suggested that educators should understand students' uncertainty about the future, provide projects requiring joint cooperation and discussion, and focus on content integration rather than rote memorization.
BMC MEDICAL EDUCATION
(2023)
Article
Plant Sciences
Minh Ly Nguyen, Xuan Cuong Mai, Nhat Ha Chu, Dang Mau Trinh, Chao-Lin Lu, Chia-Rui Shen
Summary: This study developed a DNA signaturing tool to identify cryptic species in the freshwater green algal genus Desmodesmus. This tool successfully identified 19 species and 24 strain groups, with a signature sequence that could represent almost 90% of Desmodesmus strains. The DNA signaturing tool showed high efficacy in strain identification and accurate classification of cryptic species within the genus Desmodesmus.
Article
Plant Sciences
Xuan Cuong Mai, Chia-Rui Shen, Chao-Lin Liu, Dang Mau Trinh, Minh Ly Nguyen
Summary: Species identification of Scenedesmus-like microalgae, including Desmodesmus, Tetradesmus, and Scenedesmus, is difficult due to their morphological and genetic similarity. In this study, we developed a DNA signaturing tool for Desmodesmus and constructed a DNA signaturing database for Tetradesmus. This database contains species-specific nucleotide sequences that can identify Tetradesmus strains based on their ITS2 sequences. Our findings also provide insights into the phylogenetic relationships among Scenedesmus-like species and suggest splitting Desmodesmus into two subgenera. The integration of DNA signaturing into a database allows for efficient identification of Scenedesmus-like species through BLAST.
JOURNAL OF PHYCOLOGY
(2023)
Article
Polymer Science
Jyuhn-Huarng Juang, Chen-Ling Chen, Chen-Wei Kao, Chen-Yi Chen, Chia-Rui Shen, Jiun-Jie Wang, Zei-Tsan Tsai, I-Ming Chu
Summary: The study investigated the histological development of MIN6 cell grafts in subcutaneous space and correlated it with imaging findings. The results showed that the originally transplanted MIN6 cells differentiated and proliferated from day 21, which was consistent with the bioluminescence and magnetic resonance imaging results.
Review
Chemistry, Analytical
Chia-Hsuan Cheng, Hiromi Yatsuda, Mikihiro Goto, Jun Kondoh, Szu-Heng Liu, Robert Y. L. Wang
Summary: Point-of-care testing (POCT) has seen explosive growth in the last two decades, with a focus on minimal sample handling, fast results, and low-cost small-sized devices. Shear horizontal surface acoustic wave (SH-SAW) biosensors have emerged as an effective solution for complete whole blood measurements in less than 3 minutes. This review provides an overview of the commercially successful SH-SAW biosensor system for medical use, highlighting its unique features such as disposable test cartridges, mass-produced bio-coatings, and palm-sized readers.
Article
Immunology
Peng Xu, Wei Tong, Chen-Yen Kuo, Han-Hsiang Chen, Robert Y. L. Wang
Summary: This study investigates the potential roles of Upf1 in EV-A71-infected cells. The results suggest that Upf1 is a host protein unfavorable for EV-A71 replication. Knockdown of Upf1 protein increases viral RNA expression and production of progeny virus, while overexpression of Upf1 protein decreases viral RNA expression and production of progeny virus. Additionally, EV-A71 infection suppresses host NMD activity.
MICROBES AND INFECTION
(2023)
Article
Virology
Jiun-Wen Wang, Yi-Jung Li, Hsin-Hsu Wu, Hsiang-Hao Hsu, Ming-Yang Chang, Robert Y. L. Wang, Ya-Chung Tian
Summary: BK polyomavirus is associated with uro-thelial carcinoma, and TAg and tAg proteins of Merkel cell polyomavirus can induce cell migration through the ERK signaling pathway. The expression of TAg and tAg also regulates the focal adhesion molecules in renal proximal tubular cells. Moreover, TAg regulates MMP-9 expression and activity through ERK signaling, while MMP-2 is regulated through an ERK-independent pathway.
Meeting Abstract
Ophthalmology
Yi-Hsing Chen, Chia-Rui Shen, Yih-Shiou Hwang
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
