Article
Chemistry, Applied
Thomas H. Jovic, Tamsin Nicholson, Hari Arora, Kim Nelson, Shareen H. Doak, Iain S. Whitaker
Summary: Nanocelluloses have significant potential in bioprinting for tissue engineering, particularly for cartilage. Different structural formulations of nanocelluloses have been characterized and tested for printability, chondrogenicity, and biocompatibility. The crystalline nanocellulose formulation showed the best biological promise for bioprinting cartilage.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Zhongrun Yuan, Sa Liu, Wenjing Song, Ying Liu, Gangyuan Bi, Renjian Xie, Li Ren
Summary: Galactose has been found to promote chondrogenesis and cartilage matrix formation, which suggests its potential for cartilage repair and regeneration.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Materials Science, Biomaterials
Jingming Gao, Xian Xu, Xiaoye Yu, Ye Fu, Hongjie Zhang, Siyi Gu, Dinglingge Cao, Quanyi Guo, Liming Xu, Jiandong Ding
Summary: Magnetic resonance imaging (MRI) is a promising non-invasive method for evaluating cartilage regeneration. In vitro experiments were conducted to investigate the quantitative relationship between MRI features and the concentrations of major components in the extracellular matrix (ECM). The study revealed that the MRI signal in biomacromolecule aqueous systems is mainly influenced by protons in hydrogens of biomacromolecule-bound water. The established quantitative relation is useful for real-time MRI-guided assessment of cartilage regeneration.
REGENERATIVE BIOMATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Elke Gossla, Anne Bernhardt, Robert Tonndorf, Dilbar Aibibu, Chokri Cherif, Michael Gelinsky
Summary: Replacement of damaged cartilage tissue with tissue engineering faces challenges due to the limited self-healing capacity of the non-vascularized tissue. A new approach using chitosan flock scaffolds combined with alginate hydrogel has been developed to support chondrogenic differentiation of human chondrocytes, showing promising results in gene expression and extracellular matrix production for cartilage regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Yifei Chen, Chenyue Zhang, Shiyong Zhang, Hexu Qi, Donghui Zhang, Yifei Li, Jie Fang
Summary: This review elaborates on the advances of biological scaffold technologies in artificial articular cartilage (AC) and introduces several successful applications of artificial AC built by these techniques.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Hao Cheng, Jie Cao, Wenru Liu, Jun Mei, Jing Xie
Summary: This study characterized an active film made of sodium alginate (SA)-locust bean gum (LBG) containing daphnetin-based film. The addition of daphnetin improved the flexibility and antibacterial activity of the cling film. The results suggest that daphnetin in SA-LBG film could be used to develop food-grade packaging material with antioxidant and antibacterial properties.
Article
Biochemistry & Molecular Biology
Lei Xu, Fenbo Ma, Jun Huang, Ka Li Frankie Leung, Chenghe Qin, William Weijia Lu, X. Edward Guo, Bin Tang
Summary: The novel hydrogel Alg/MH-Sr shows significant therapeutic effects on cartilage lesions by accelerating cartilage tissue repair through inhibiting cellular and tissue senescence.
Article
Orthopedics
Makayla Risch, Jeremiah T. Easley, Erin G. McCready, Kevin L. Troyer, James W. Johnson, Benjamin C. Gadomski, Kirk C. McGilvray, John D. Kisiday, Brad B. Nelson
Summary: There are topographic variations in mechanical, morphological, and biochemical properties across knee cartilage surfaces in sheep, with significant differences between different surface locations and regions within each location.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Orthopedics
Mikiko Kobayashi-Miura, Harumi Osago, Yoshifumi Hamasaki, Ikuko Takano, Mitsuki Akiho, Mineyoshi Hiyoshi, Nobumasa Hara
Summary: This study found a significant decrease in GAG content in articular cartilage with aging in female normal rats, which may explain the higher prevalence and more severe osteoarthritis in females.
Review
Chemistry, Applied
Sumit Murab, Aastha Gupta, Malgorzata Katarzyna Wlodarczyk-Biegun, Anuj Kumar, Patrick van Rijn, Patrick Whitlock, Sung Soo Han, Garima Agrawal
Summary: This review highlights the importance of alginate-based hydrogel inks for fabricating 3D printed scaffolds for bone and cartilage regeneration. The fundamentals of direct extrusion 3D bioprinting method are discussed, and a comprehensive overview of various alginate-based hydrogel ink formulations that have been used so far is provided. The requirements of hydrogel inks and 3D printed scaffolds to achieve similarity to the native tissue environment are summarized.
CARBOHYDRATE POLYMERS
(2022)
Article
Polymer Science
Alan Avila-Ramirez, Kevin Catzim-Rios, Carlos Enrique Guerrero-Beltran, Erick Ramirez-Cedillo, Wendy Ortega-Lara
Summary: This study presents the preparation of a novel ink for bone and cartilage tissue restoration, focusing on the synthesis of bioceramic materials, integration into a biopolymeric base matrix, and characterization of functional composite materials for future manufacturing using techniques such as 3D printing.
Article
Polymer Science
Amin Soltani, Ramin Raeesi, Abbas Taheri, An Deng, Mehdi Mirzababaei
Summary: The study showed that increasing the dosage of sodium alginate (SA) and/or extending the curing duration can enhance the uniaxial compressive strength (UCS) of rubberized soil blends.
Article
Engineering, Biomedical
Yun Chu, Lei Huang, Wangping Hao, Tongtong Zhao, Haitao Zhao, Wen Yang, Xin Xie, Lin Qian, Yanyan Chen, Jianwu Dai
Summary: 3D bioprinting of double crosslinked alginate hydrogel promotes MSC differentiation into cartilage and demonstrates good mechanical properties, stability, and printing accuracy.
BIOMEDICAL MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Guoshuang Zheng, Chundong Xue, Fang Cao, Minghui Hu, Maoyuan Li, Hui Xie, Weiting Yu, Dewei Zhao
Summary: In this study, an alginate/collagen (ALG/COL) hybrid hydrogel with different guluronate/mannuronate acid ratios was developed. The mechanical stiffness of the hydrogel was significantly affected by the G/M ratios of alginate. Chondrocytes cultured on the Mid-G/M hydrogels exhibited better viability and phenotype preservation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Elena Solovieva, Anastasiya Yu Teterina, Olga Klein, Vladimir S. Komlev, Andrey A. Alekseev, Andrey A. Panteleyev
Summary: The study developed bilayer alginate-based scaffolds with biological and mechanical properties comparable to traditional scaffolds but surpassing them in cost efficiency and vascularization ability.
BIOMEDICAL MATERIALS
(2021)
Article
Cell Biology
Cristiane C. Denardin, Leo A. M. Martins, Mariana M. Parisi, Moema Queiroz Vieira, Silvia R. Terra, Florencia M. Barbe-Tuana, Radovan Borojevic, Marcia Vizzotto, Tatiana Emanuelli, Fatima Costa Rodrigues Guma
CELL BIOLOGY AND TOXICOLOGY
(2017)
Article
Dentistry, Oral Surgery & Medicine
L. L. Bonato, V. Quinelato, R. Borojevic, A. R. Vieira, A. Modesto, J. M. Granjeiro, R. Tesch, P. L. Casado
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
(2017)
Article
Engineering, Biomedical
Andre L. Rossi, Mariana M. Longuinho, Marcelo N. Tanaka, Marcos Farina, Radovan Borojevic, Alexandre M. Rossi
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2018)
Article
Chemistry, Multidisciplinary
Ana R. Ribeiro, Arijita Mukherjee, Xuan Hu, Shayan Shafien, Reza Ghodsi, Kun He, Sara Gemini-Piperni, Canhui Wang, Robert F. Klie, Tolou Shokuhfar, Reza Shahbazian-Yassar, Radovan Borojevic, Luis A. Rocha, Jose M. Granjeiro
Article
Zoology
Michelle Klautau, Fernanda F. Cavalcanti, Radovan Borojevic
Article
Surgery
Luiz Charles-de-Sa, Natale Ferreira Gontijo-de-Amorim, Christina Maeda Takiya, Radovan Borojevic, Donatella Benati, Paolo Bernardi, Andrea Sbarbati, Gino Rigotti
AESTHETIC SURGERY JOURNAL
(2018)
Article
Chemistry, Multidisciplinary
S. A. Alves, A. R. Ribeiro, S. Gemini-Piperni, R. C. Silva, A. M. Saraiva, P. E. Leite, G. Perez, S. M. Oliveira, J. R. Araujo, B. S. Archanjo, M. E. Rodrigues, M. Henriques, J. -P. Celis, T. Shokuhfar, R. Borojevic, J. M. Granjeiro, L. A. Rocha
Article
Cell & Tissue Engineering
Ricardo de Souza Tesch, Esther Rieko Takamori, Karla Menezes, Rosana Bizon Vieira Carias, Claudio Leonardo Milione Dutra, Marcelo de Freitas Aguiar, Tania Salgado de Sousa Torraca, Alexandra Cristina Senegaglia, Carmen Lucia Kuniyoshi Rebelatto, Debora Regina Daga, Paulo Roberto Slud Brofman, Radovan Borojevic
STEM CELL RESEARCH & THERAPY
(2018)
Article
Biochemistry & Molecular Biology
Mariana Ilha, Ketlen da Silveira Moraes, Francieli Rohden, Leo Anderson Meira Martins, Radovan Borojevic, Guido Lenz, Florencia Barbe-Tuana, Fatima Costa Rodrigues Guma
JOURNAL OF CELLULAR BIOCHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Qian Chu, Thomas F. Martinez, Sammy Weiser Novak, Cynthia J. Donaldson, Dan Tan, Joan M. Vaughan, Tina Chang, Jolene K. Diedrich, Leo Andrade, Andrew Kim, Tong Zhang, Uri Manor, Alan Saghatelian
NATURE COMMUNICATIONS
(2019)
Article
Biochemical Research Methods
Cara R. Schiavon, Tong Zhang, Bing Zhao, Andrew S. Moore, Pauline Wales, Leonardo R. Andrade, Melissa Wu, Tsung-Chang Sung, Yelena Dayn, Jasmine W. Feng, Omar A. Quintero, Gerald S. Shadel, Robert Grosse, Uri Manor
Article
Multidisciplinary Sciences
Romain Chassefeyre, Tai Chaiamarit, Adriaan Verhelle, Sammy Weiser Novak, Leonardo R. Andrade, Andre D. G. Leitao, Uri Manor, Sandra E. Encalada
Summary: The study reveals the role of the small endolysosomal guanosine triphosphatase Arl8b in the formation of pathologic PrP endoggresomes through the ARESTA mechanism. Inhibiting ARESTA reduces endoggresome formation, rescues calcium influx, and prevents neuronal death.
Article
Cell Biology
Sarah K. Schroeder, Herdit M. Schueler, Kamilla Petersen, Cinzia Tesauro, Birgitta R. Knudsen, Finn S. Pedersen, Frederike Krus, Eva M. Buhl, Elke Roeb, Martin Roderfeld, Radovan Borojevic, Jamie L. Almeida, Ralf Weiskirchen
Summary: The GRX cell line, established from livers of mice infected with Schistosoma mansoni, displays a myofibroblast phenotype and can acquire a fat-storing lipocyte phenotype. Genetic characterization revealed chromosomal aberrations and the presence of viral particles belonging to the Retroviridae family. The cell line lacks S. mansoni-specific glycostructures. These findings are important for cell authentication and to avoid misinterpretation of experimental results.
Article
Multidisciplinary Sciences
Karla Menezes, Barbara Gomes Rosa, Catarina Freitas, Aline Silva da Cruz, Raphael de Siqueira Santos, Marcos Assis Nascimento, Daiana Vieira Lopes Alves, Martin Bonamino, Maria Isabel Rossi, Radovan Borojevic, Tatiana Coelho-Sampaio
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Monique de Barros Elias, Felipe Leite Oliveira, Fatima Costa Rodrigues Guma, Renata Brum Martucci, Radovan Borojevic, Anderson Junger Teodoro