Article
Cell Biology
Jun Ochiai, Larakaye Villanueva, Hope Niihara, Yutaka Niihara, Joan Oliva
Summary: This study developed a method to engineer multilayer adipose stem cell cell sheets (ASCCSs) using chemically defined/serum-free culture media, which can differentiate into osteoblasts and chondrocytes. The number of cells per cell sheet was estimated using cell sheet transmittance, providing important non-invasive information.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Minh Quang Nguyen, Hue T. H. Bui, Anh Nguyen Thi Tuyet, Trinh Thi Hong Nhung, Duc M. Hoang, Nguyen Thanh Liem, Van T. Hoang
Summary: In a standardized xeno- and serum-free culture platform, cryopreserved UC-MSCs exhibit similar characteristics to fresh UC-MSCs, showing comparable viability, proliferation capacity, immunophenotype, and differentiation potential. However, culture-rescued cells appear to have a more potent immunosuppressive potential compared to freshly thawed cells.
CELL TRANSPLANTATION
(2021)
Article
Cell Biology
Maria Serena Piccinno, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, Ilenia Mastrolia, Chiara Chiavelli, Ilaria Castelli, Daniela Bernabei, Mara Tagliazucchi, Elisa Bonetti, Francesca Lolli, Giorgio De Santis, Massimo Dominici, Elena Veronesi
Summary: This study investigates the use of adipose-derived mesenchymal stromal/stem cells (AD-MSCs) in autologous fat transfer (AFT) for tissue reconstruction. The results show that AD-MSCs can improve graft persistence and reduce tissue resorption after AFT. The implanted AD-MSCs can persist and differentiate into mature adipocytes, leading to better outcomes in terms of graft weight and reduced cyst formation. This suggests a long-term protective and anti-inflammatory/proangiogenic role of AD-MSCs in autologous AFT tissue resorption.
Article
Cell & Tissue Engineering
Phuong T. M. Dam, Van T. Hoang, Hue Thi Hong Bui, Le Minh Hang, Duc M. Hoang, Hoang Phuong Nguyen, Ha Thi Lien, Huong Thi Thanh Tran, Xuan-Hung Nguyen, Liem Nguyen Thanh
Summary: An increased expression of negative markers, decreased growth ability, chromosomal abnormalities, and senescent feature were observed in ADMSCs expanded in PS media compared to those cultured in SM media. This study suggests that SM media is more suitable for the expansion of therapeutic ADMSCs, highlighting the importance of quality control for therapeutic MSC products, especially at advanced passages and with older donors.
STEM CELL REVIEWS AND REPORTS
(2021)
Review
Cell Biology
Conor A. Crowley, William P. W. Smith, K. T. Matthew Seah, Soo-Keat Lim, Wasim S. Khan
Summary: Adipose tissue senescence is linked to obesity and aging-related diseases, and adipose-derived stem cells are used in regenerative medicine. Cryopreservation allows for greater flexibility in tissue use. Trehalose, a sugar synthesized by lower organisms, has been tested as a cryoprotective agent, showing comparable effectiveness to DMSO when introduced into cells using appropriate methods.
Review
Cell & Tissue Engineering
Xiuyi Huang, Yunchong Liu, Zilun Li, Lilach O. Lerman
Summary: Mesenchymal stem/stromal cells (MSCs) have therapeutic potential in treating metabolic syndrome (MetS) by inhibiting inflammation, improving insulin resistance, regulating glycolipid metabolism, and protecting organ function. However, several challenges remain to overcome, such as the unclear merits of autologous versus allogeneic MSCs sources, the distinct characteristics and relative efficacy of MSCs harvested from different tissue sources, and the potential safety concerns of approaches to improve therapeutic efficacy. Larger-scale studies are needed to determine the therapeutic efficacy and safety of MSCs for clinical application.
Article
Biochemistry & Molecular Biology
Nicolo Costantino Brembilla, Ali Modarressi, Dominik Andre-Levigne, Estelle Brioudes, Florian Lanza, Hubert Vuagnat, Stephane Durual, Laurine Marger, Wolf-Henning Boehncke, Karl-Heinz Krause, Olivier Preynat-Seauve
Summary: This study evaluates the influence of a gelatin sponge on adipose-derived stromal cells (ASC). The results show that the gelatin sponge strongly influences the transcriptome of ASC and acted as a concentrator and reservoir of the regenerative ASC secretome. The gelatin sponge also increased angiogenesis in vivo and promoted wound healing.
Article
Cell & Tissue Engineering
Van T. Hoang, Quynh-Mai Trinh, Dam Thi Minh Phuong, Hue Thi Hong Bui, Le Minh Hang, Nguyen Thi Hong Ngan, Nguyen Thi Tuyet Anh, Phung Yen Nhi, Trinh Thi Hong Nhung, Ha Thi Lien, Tu Dac Nguyen, Liem Nguyen Thanh, Duc M. Hoang
Summary: The study established a standardized method for expanding MSCs using xeno and serum-free commercial media, demonstrating that MSCs from different sources can be stably expanded and retain their functional characteristics. The results showed that MSCs successfully differentiated into various lineages and met quality standards for therapeutic applications after pilot expansion to clinical scale, highlighting the potential for large-scale application of MSCs in therapy.
Review
Cell Biology
Vadim I. Chechekhin, Konstantin Yu. Kulebyakin, Romesh I. Kokaev, Pyotr A. Tyurin-Kuzmin
Summary: Adipose tissue relies on the regulation of multipotent mesenchymal stromal cells (MSCs), which serve as both precursors for new adipocytes and key paracrine regulators of adipose tissue homeostasis. The activity of MSCs is tightly controlled, regulating aspects such as proliferation, differentiation, and signal molecule production through modulation of hormonal sensitivity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cell Biology
Beverly Brooks, Dominique Ebedes, Ahsan Usmani, Joaquin Vega Gonzales-Portillo, Daniel Gonzales-Portillo, Cesario Borlongan
Summary: Ischemic brain injury is a major cause of death worldwide with limited treatment options. Stem cell-based regenerative therapies, specifically mesenchymal stromal cells, show promise in stroke treatment, but further research is needed to fully understand their cell function.
Article
Plant Sciences
Heidi D. D. Kreckel, Fionna M. D. Samuels, Remi Bonnart, Gayle M. M. Volk, Dominik G. G. Stich, Nancy E. E. Levinger
Summary: Cryopreservation is a cost-effective method for long-term preservation of vegetatively propagated crops. Using Raman microscopy, this study visualizes the distribution of dimethyl sulfoxide (DMSO) in Mentha x piperita shoot tips and finds that DMSO fully penetrates the tissue within 10 minutes, possibly accumulating in specific regions due to its interaction with cellular components.
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alec Gessner, Benjamin Koch, Kevin Klann, Dominik C. Fuhrmann, Samira Farmand, Ralf Schubert, Christian Muench, Helmut Geiger, Patrick C. Baer
Summary: The study aimed to characterize EVs from hypoxic preconditioned ASCs, showing that hypoxic conditions can alter the protein cargo and enhance the antioxidative potential of EVs, providing potential therapeutic options for organ regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Fionna M. D. Samuels, Dominik G. Stich, Remi Bonnart, Gayle M. Volk, Nancy E. Levinger
Summary: Cryoprotectants allow cells to be frozen and preserved for years, but variations in cryoprotectant concentration within different cells and tissues may lead to different protective effects. Using coherent anti-Stokes Raman scattering microscopy can help directly observe the distribution of cryoprotectants within living cells.
Article
Medicine, General & Internal
Ulrica Askelof, Ola Andersson, Magnus Domellof, Anders Fasth, Boubou Hallberg, Lena Hellstrom-Westas, Karin Pettersson, Magnus Westgren, Ingela E. Wiklund, Cecilia Gotherstrom
Article
Biology
Christopher M. Groen, Cormac Mcgrath, Katherine A. Campbell, Cecilia Gotherstrom, Anthony J. Windebank, Natalia Landazuri
Article
Cell & Tissue Engineering
Asa Ekblad, Magnus Westgren, Magdalena Fossum, Cecilia Goetherstroem
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2018)
Article
Cell & Tissue Engineering
Fawaz Abomaray, Sebastian Gidlof, Bartosz Bezubik, Mikael Engman, Cecilia Gotherstrom
STEM CELLS INTERNATIONAL
(2018)
Correction
Cell Biology
Shahla Hamza Al-Saqi, Mohammed Saliem, Hernan Concha Quezada, Asa Ekblad, Aino Fianu Jonasson, Outi Hovatta, Cecilia Gotherstrom
CELL AND TISSUE BANKING
(2019)
Article
Cell & Tissue Engineering
Clara Ibel Chamorro, Muhammad Asghar, Asa Ekblad, Anna Farnert, Cecilia Gotherstrom, Magdalena Fossum
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2019)
Review
Cell & Tissue Engineering
Asa Ekblad-Nordberg, Lilian Walther-Jallow, Magnus Westgren, Cecilia Gotherstrom
STEM CELLS TRANSLATIONAL MEDICINE
(2020)
Article
Cell & Tissue Engineering
Chenhong Lin, Cinzia Calzarossa, Teresa Fernandez-Zafra, Jia Liu, Xiaofei Li, Asa Ekblad-Nordberg, Erika Vazquez-Juarez, Simone Codeluppi, Lena Holmberg, Maria Lindskog, Per Uhlen, Elisabet Akesson
STEM CELL RESEARCH & THERAPY
(2020)
Review
Endocrinology & Metabolism
Cecilia Gotherstrom, Lilian Walther-Jallow
CURRENT OSTEOPOROSIS REPORTS
(2020)
Correction
Cell & Tissue Engineering
Chenhong Lin, Cinzia Calzarossa, Teresa Fernandez-Zafra, Jia Liu, Xiaofei Li, Asa Ekblad-Nordberg, Erika Vazquez-Juarez, Simone Codeluppi, Lena Holmberg, Maria Lindskog, Per Uhlen, Elisabet Akesson
STEM CELL RESEARCH & THERAPY
(2020)
Article
Hematology
Sara Marie Larsson, Andreas Hillarp, Pia Karlsland akeson, Lena Hellstrom-Westas, Magnus Domellof, Ulrica Askelof, Cecilia Gotherstrom, Ola Andersson
Summary: This study establishes reference intervals for sTfR from birth to 12 months in a large group of infants in a low-risk area for iron deficiency. sTfR could be a valuable tool for infant iron status diagnostics as it showed no covariation with CRP at birth, 4 months, or 12 months.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2021)
Article
Cell Biology
Chenhong Lin, Asa Ekblad-Nordberg, Jakob Michaelsson, Cecilia Gotherstrom, Chia-Chen Hsu, Hua Ye, Jan Johansson, Anna Rising, Erik Sundstrom, Elisabet Akesson
Summary: The study analyzed the immune response to recombinant spider silk protein and hyaluronic acid hydrogel in combination with cell therapy for spinal cord injury. The activation of immune cells in human peripheral blood mononuclear cells cultured with these biomaterials and grafted human neural stem/progenitor cells was evaluated. The study highlighted the importance of early evaluation of human compatibility of novel biomaterials to minimize rejection risk.
Article
Pediatrics
Sara Marie Larsson, Lena Hellstrom-Westas, Andreas Hillarp, Pia Karlsland Akeson, Magnus Domellof, Ulrica Askelof, Cecilia Gotherstrom, Ola Andersson
Summary: This study aimed to establish reference intervals for haemoglobin and red blood cell biomarkers in infants based on a large cohort in Sweden. The reference intervals varied with infants' age, indicating the necessity of age-specific definitions. Despite favorable conditions in infancy, a considerable number of infants were classified as anaemic at 12 months according to WHO criteria, warranting further investigation.
ARCHIVES OF DISEASE IN CHILDHOOD
(2022)
Article
Cell Biology
Fawaz Abomaray, Arnika Kathleen Wagner, Michael Chrobok, Asa Ekblad-Nordberg, Sebastian Gidlof, Evren Alici, Cecilia Gotherstrom
Summary: The study suggests that ectopic mesenchymal stromal cells (MSC) may not contribute to reduced immunosurveillance in endometriosis through their inhibitory effects on natural killer (NK) cells, indicating that inhibition of NK cells in the pelvic cavity of women with endometriosis may be influenced by other factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)