Article
Biochemistry & Molecular Biology
Lin Chen, Jia Xin, Heyun Song, Fei Xu, Hui Yang, Heng Sun, Mei Yang
Summary: In this study, 57 putative LEA genes were identified in lotus, indicating their important roles in seed development and involvement in ABA signaling and seed maturation processes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
David Lalanne, Jaiana Malabarba, Joseph Ly Vu, Michaela Hundertmark, Julien Delahaie, Olivier Leprince, Julia Buitink, Jerome Verdier
Summary: The study investigated the role of the ABI3 gene in seed maturation in Medicago, revealing three MtABI3 expression isoforms with distinct regulatory functions and gene clusters. Complementation experiments in Arabidopsis showed that all isoforms restored seed viability and desiccation tolerance, highlighting the importance of understanding the ABI3 network in seed development.
Article
Plant Sciences
Aline Kadri, Ghislaine Grenier De March, Francois Guerineau, Viviane Cosson, Pascal Ratet
Summary: The overexpression of the heterologous Arabidopsis WUS gene was found to enhance callus and embryogenesis in Medicago truncatula explants, providing a promising tool for inducing somatic embryogenesis and improving regeneration efficiency in recalcitrant crops.
Article
Plant Sciences
Andrei A. Kudriashov, Natalia S. Zlydneva, Elena P. Efremova, Varvara E. Tvorogova, Ludmila A. Lutova
Summary: CLE peptides play a role in plant development and can be regulated by WOX transcription factors. This study identified three CLE peptides that could potentially regulate the MtWOX9-1 gene and stimulate somatic embryogenesis in Medicago truncatula. However, none of these peptides affected the expression of MtWOX9-1 in embryogenic calli. One CLE gene, MtCLE16, was found to decrease somatic embryogenesis intensity when overexpressed, while overexpression of MtCLE08 suppressed the expression of MtWOX13a, a supposed antagonist of somatic embryo development. These findings contribute to the search for new regeneration regulators for plant transformation.
Article
Plant Sciences
Chang Wang, Zhe Liu, Zicheng Wang, Wenhui Pang, Long Zhang, Zhaozhu Wen, Yiran Zhao, Juan Sun, Zeng-Yu Wang, Chao Yang
Summary: Autotoxicity is a phenomenon in which a plant inhibits the growth of the same species through the release of toxic chemical compounds. This study found that the leguminous plant M. truncatula exhibits autotoxicity and allelopathic effects, providing a new model species for further research on autotoxicity and allelopathy.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Zhijuan Chen, Joseph Ly Vu, Benoit Ly Vu, Julia Buitink, Olivier Leprince, Jerome Verdier
Summary: Legume seeds are crucial sources of proteins, minerals, and vitamins for human and animal diets, and their performance traits are influenced by the maternal environment. Heat stress during seed development can impact seed weight and germination capacity, with genome-wide association studies identifying potential genes involved in regulating these traits. MtMIEL1, a zinc finger family gene, was found to be associated with germination speed in heat-stressed seeds, highlighting its role in regulating seed traits under stress conditions.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Naoto Sano, Jaiana Malabarba, Zhijuan Chen, Sylvain Gaillard, David Windels, Jerome Verdier
Summary: Desiccation tolerance (DT) is an important adaptation of land plants to water-deficient conditions, mainly observed in reproductive parts such as seeds. In this study, chromatin dynamics related to the re-inducibility of DT were analyzed in Medicago truncatula radicles. Comparative transcriptome and ATAC-Seq analyses revealed that DT re-induction-related genes were transcriptionally regulated at the chromatin level. ChIP-Seq analyses also showed the presence of H3K27me3 marks on DT re-induction-related gene sequences in later radicle stages.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Marina E. Battaglia, Ana Valeria Martinez-Silva, Yadira Olvera-Carrillo, Tzvetanka D. Dinkova, Alejandra A. Covarrubias
Summary: The PvLEA6-3'UTR enhances the loading of GUS reporter transcript on polysomes under water deficit conditions, promoting efficient protein synthesis. Specific conserved motifs in this region stimulate translation, while a specific interaction with cellular proteins was observed.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Immunology
Terhi Maula, Nelli Vahvelainen, Helena Tossavainen, Tuuli Koivunen, Marja T. Pollanen, Anders Johansson, Perttu Permi, Riikka Ihalin
Summary: Late embryogenesis abundant (LEA) proteins play important roles in responding to stressful conditions, with many being intrinsically disordered proteins and highly hydrophilic. BilRI, an outer membrane interleukin receptor I, shares sequence similarity with LEA proteins and is expressed more at decreased temperatures.
Article
Plant Sciences
Andrea Pagano, Laura Kunz, Antje Dittmann, Susana De Sousa Araujo, Anca Macovei, Shraddha Shridhar Gaonkar, Federico Sincinelli, Hisham Wazeer, Alma Balestrazzi
Summary: This study investigated the changes in the protein profile of Medicago truncatula seeds during the rehydration-dehydration cycle of a vigorization treatment and post-priming imbibition. The results revealed changes in protein accumulation under dehydration stress and differential regulation during post-priming imbibition. The study also demonstrated that primed seeds were able to cope with genotoxic damage induced by 2'-deoxyinosine, and assessed the expression profiles of genes involved in seed repair pathways.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Orarat Ginsawaeng, Michal Gorka, Alexander Erban, Carolin Heise, Franziska Brueckner, Rainer Hoefgen, Joachim Kopka, Aleksandra Skirycz, Dirk K. Hincha, Ellen Zuther
Summary: During seed germination, desiccation tolerance is lost in the radicle with progressing radicle protrusion and seedling establishment, accompanied by comprehensive changes in the metabolome and proteome. Thermo-stable proteins are associated with a multitude of biological processes, including development, protein, RNA, and stress. The network analysis revealed a highly connected LEA protein network during the course of germination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Weibo Geng, Yanan Wang, Jing Zhang, Zhonghui Liu, Xingyun Chen, Liting Qin, Long Yang, Heng Tang
Summary: This study systematically investigated the NtLEA gene family in tobacco and identified 123 NtLEA genes, which were analyzed in detail. The study found that these genes showed significant induced expression under two different abiotic stresses and exhibited different expression patterns. Additionally, the majority of NtLEA proteins were shown to have the potential function to enhance tobacco resistance to abiotic stress.
Article
Plant Sciences
LiTing Ma, Ting Zhu, HaiRong Wang, Hao Zhou, LeiLei Shao, Qin Ding, DaZhong Zhang, LingJian Ma
Summary: Late embryogenesis-abundant (LEA) proteins, important gene products in plants, play crucial roles in regulating growth, development, and stress responses. In the study on Brachypodium distachyon L., 67 BdLEAs were identified, categorized into six subfamilies, with majority involved in stress response. This research sheds light on the characteristics and evolutionary aspects of BdLEA family, facilitating future functional studies on these proteins in model plant B. distachyon.
FUNCTIONAL PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Karamjeet K. Singh, Steffen P. Graether
Summary: Late embryogenesis abundant (LEA) proteins play crucial roles in stress response and stress tolerance, with AtLEA3-4 showing specific binding abilities to metal ions and membrane mimic. Biochemical and structural analyses revealed that LEA3 proteins, although disordered in solution, possess regions with propensity for order.
Article
Plant Sciences
Zhongjing Zhou, Baogang Lin, Jinjuan Tan, Pengfei Hao, Shuijin Hua, Zhiping Deng
Summary: In this study, a quantitative proteomic approach was used to compare differentially expressed proteins in high oil content and low oil content Brassica napus seeds. The results showed that these differentially expressed proteins were mainly involved in lipid biosynthesis and metabolism, photosynthesis, and nutrient reservoir activity. Western blot analysis confirmed that overexpression of a late embryogenesis abundant protein (BnLEA57) can enhance oil content. This study provides new insights into the molecular regulatory mechanism of seed oil content in B. napus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Guillaume Quang N'Guyen, Roxane Raulo, Antoine Porquier, Beatrice Iacomi, Sandra Pelletier, Jean-Pierre Renou, Nelly Bataille-Simoneau, Claire Campion, Bruno Hamon, Anthony Kwasiborski, Justine Colou, Abdelilah Benamar, Pietrick Hudhomme, David Macherel, Philippe Simoneau, Thomas Guillemette
Summary: This study indicates that the indolic phytoalexin primarily targets mitochondrial functions in fungal cells. Treatment of A. brassicicola with phytoalexin disrupted the mitochondrial membrane potential and led to a significant and rapid decrease in oxygen consumption rates. Secondary effects, such as Reactive oxygen species production, changes in lipid and endoplasmic reticulum homeostasis were induced. Consequently, the fungus has to adapt its metabolism to protect itself against the toxic effects of these molecules, especially via the activation of high osmolarity glycerol and cell wall integrity signaling pathways and by induction of the unfolded protein response.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Elise Bizouerne, Julia Buitink, Benoit Ly Vu, Joseph Ly Vu, Eddi Esteban, Asher Pasha, Nicholas Provart, Jerome Verdier, Olivier Leprince
Summary: By analyzing the RNA sequences of different seed tissues, several gene modules associated with the physiological traits of seeds were identified. Dormancy is gradually released during fruit ripening, accompanied by an increase in seed vigor. Some gene modules involve gene expression regulation related to seed vigor.
Article
Plant Sciences
Kevin Cartelier, Delphine Aime, Joseph Ly Vu, Lucie Combes-Soia, Valerie Labas, Jean-Marie Prosperi, Julia Buitink, Karine Gallardo, Christine Le Signor
Summary: Genes related to transcription, DNA repair, and signal transduction were found to be enriched in the seed protein composition of legumes, with many being stress responsive. Additionally, over-represented genes were related to pathways involved in synthesizing essential amino acids such as methionine and lysine.
Article
Plant Sciences
Zhijuan Chen, Joseph Ly Vu, Benoit Ly Vu, Julia Buitink, Olivier Leprince, Jerome Verdier
Summary: Legume seeds are crucial sources of proteins, minerals, and vitamins for human and animal diets, and their performance traits are influenced by the maternal environment. Heat stress during seed development can impact seed weight and germination capacity, with genome-wide association studies identifying potential genes involved in regulating these traits. MtMIEL1, a zinc finger family gene, was found to be associated with germination speed in heat-stressed seeds, highlighting its role in regulating seed traits under stress conditions.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
David Lalanne, Jaiana Malabarba, Joseph Ly Vu, Michaela Hundertmark, Julien Delahaie, Olivier Leprince, Julia Buitink, Jerome Verdier
Summary: The study investigated the role of the ABI3 gene in seed maturation in Medicago, revealing three MtABI3 expression isoforms with distinct regulatory functions and gene clusters. Complementation experiments in Arabidopsis showed that all isoforms restored seed viability and desiccation tolerance, highlighting the importance of understanding the ABI3 network in seed development.
Article
Multidisciplinary Sciences
Gregoire Bianchetti, Cecile Baron, Aurelien Carrillo, Solenne Berardocco, Nathalie Marnet, Marie-Helene Wagner, Didier Demilly, Sylvie Ducournau, Maria J. Manzanares-Dauleux, Francoise Le Caherec, Julia Buitink, Nathalie Nesi
Summary: In order to adapt agriculture to climate change, seed production must increase resilience to abiotic stress factors and pathogen proliferation. Oilseed rape is one of the largest oleaginous crops globally, but faces challenges from drought and clubroot disease. Metabolic profiling was conducted on seed samples from different stress conditions to better understand the impact on seed quality and germination traits. These insights are crucial for developing seeds that can thrive in their environment.
Article
Biotechnology & Applied Microbiology
L. Perchepied, E. Chevreau, E. Ravon, S. Gaillard, S. Pelletier, M. Bahut, P. Berthelot, R. Cournol, H. J. Schouten, E. Vergne
Summary: This study analyzed the mode of action of a major resistance gene in apple (Rvi6) in transgenic apple and pear plants interacting with two different scab species, revealing a successful intergeneric transfer of a resistance gene among Rosaceae. The resistance in pear towards V. pyrina varied depending on the strain, with one strain overcoming the resistance of most transgenic pear clones. Comparative transcriptomic analyses showed different molecular mechanisms downstream of pathogen recognition by Rvi6 in apple and pear, leading to the induction of various defense responses.
Article
Multidisciplinary Sciences
Gregoire Bianchetti, Vanessa Clouet, Fabrice Legeai, Cecile Baron, Kevin Gazengel, Aurelien Carrillo, Maria J. Manzanares-Dauleux, Julia Buitink, Nathalie Nesi
Summary: Oilseed rape is the third largest oil crop globally, facing unfavorable environmental conditions such as drought and clubroot disease which threaten its yield and seed quality. Combinations of different stresses lead to complex plant responses, and transcriptome datasets are essential for understanding environmental stress-resistance mechanisms in plant organs.
Article
Plant Sciences
Lili Zang, Lukasz Pawel Tarkowski, Marie-Christine Morere-Le Paven, Michel Zivy, Thierry Balliau, Thibault Clochard, Muriel Bahut, Sandrine Balzergue, Sandra Pelletier, Claudine Landes, Anis M. Limami, Francoise Montrichard
Summary: Nitrate is not only a necessary nutrient for plants, but also a signal involved in plant development. This study investigated the role of MtNPF6.8, a nitrate transporter, in regulating root growth and identified the POD isoforms responsible for changes in POD activity. It was found that MtNPF6.8 is a master mediator of the nitrate signal and its integrity is crucial for the sensitivity of the root tip to nitrate. Additionally, transcriptomic and proteomic analysis revealed the genes and proteins that respond to nitrate and provided insights into the changes in POD activity.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Fiona R. Hay, Shabnam Rezaei, Julia Buitink
Summary: Seed moisture sorption isotherms play a crucial role in predicting seed stability and understanding development conditions, making them essential for optimizing drying and storage.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Julia Zinsmeister, David Lalanne, Benoit Ly Vu, Benoit Schoefs, Justine Marchand, Thi Thu Dang, Julia Buitink, Olivier Leprince
Summary: Seed longevity is important for preserving genetic diversity, and it is acquired during late maturation through the dismantlement of chloroplasts. The gene ABI4 plays a role in coordinating this process in Medicago truncatula, as its mutation leads to increased chlorophyll retention and reduced longevity. The study also found that the shutdown of photosynthesis during maturation is crucial for the acquisition of seed longevity.
Article
Biochemistry & Molecular Biology
Claude Emmanuel Koutouan, Valerie Le Clerc, Anita Suel, Latifa Hamama, Patricia Claudel, David Halter, Raymonde Baltenweck, Philippe Hugueney, Jean-Francois Chich, Sitti Anlati Moussa, Clementine Champlain, Sebastien Huet, Linda Voisine, Sandra Pelletier, Sandrine Balzergue, Wilfried Chevalier, Emmanuel Geoffriau, Mathilde Briard
Summary: Alternaria leaf blight (ALB) is a destructive foliar disease of carrots caused by Alternaria dauci. Some carrot genotypes show partial resistance to ALB and resistance Quantitative Trait Loci (rQTL) have been identified. Metabolic and rQTL co-localization study revealed the potential involvement of specific terpenes, including camphene, alpha-pinene, alpha-bisabolene, beta-cubebene, caryophyllene, germacrene D, and alpha-humulene, in carrot resistance against ALB. Genomic and transcriptomic analyses identified differentially expressed terpene-related genes, including five terpene synthases and twenty transcription factors, in the co-localization regions between a resistant and a susceptible carrot genotype. Furthermore, alpha-humulene and caryophyllene displayed significant inhibition of mycelial growth.
Article
Plant Sciences
Elise Bizouerne, Joseph Ly Vu, Benoit Ly Vu, Isidore Diouf, Frederique Bitton, Mathilde Causse, Jerome Verdier, Julia Buitink, Olivier Leprince
Summary: This study investigated the impact of heat stress during fruit ripening on tomato seed lifespan during storage. The results showed that heat stress reduced the longevity and germination homogeneity of seeds, but there was genetic variability in the seed response to heat stress. Several QTLs associated with seed quality traits were identified, and candidate genes were proposed based on previous transcriptome and gene network data.
Article
Multidisciplinary Sciences
Elise Bizouerne, Benoit Ly Vu, Joseph Ly Vu, Jerome Verdier, Julia Buitink, Olivier Leprince
Summary: Seed vigor is an estimation of how well a seed lot can establish seedlings under various environmental conditions, with embryo and endosperm playing distinct roles in germination behavior. The genetic programs governing seed vigor traits are influenced by environmental conditions, and factors like light and temperature impact the molecular mechanisms related to seed vigor. Through RNA sequencing on differentially treated tomato seed tissues, it was found that there are distinct gene expressions and functional annotations related to seed vigor.