Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 67, Issue 1, Pages 69-82Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erv421
Keywords
Apoplast; cold stress tolerance; CYP19-4; immunophilin; increased tillering; Oryza sativa L.
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Funding
- National Research Foundation of Korea (NRF) - Korean government (MEST) [NRF-2012R1A1A2044517]
- Agricultural Biotechnology Developmental Program - Ministry of Agriculture, Food and Rural Affairs [114061-3]
- KRIBB Initiative Program
- Research Program for Agricultural Science AMP
- Technology Development of the National Academy of Agricultural Science [PJ006647]
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AtCYP19-4 (also known as CYP5) was previously identified as interacting in vitro with GNOM, a member of a large family of ARF guanine nucleotide exchange factors that is required for proper polar localization of the auxin efflux carrier PIN1. The present study demonstrated that OsCYP19-4, a gene encoding a putative homologue of AtCYP19-4, was up-regulated by several stresses and showed over 10-fold up-regulation in response to cold. The study further demonstrated that the promoter of OsCYP19-4 was activated in response to cold stress. An OsCYP19-4-GFP fusion protein was targeted to the outside of the plasma membrane via the endoplasmic reticulum as determined using brefeldin A, a vesicle trafficking inhibitor. An in vitro assay with a synthetic substrate oligomer confirmed that OsCYP19-4 had peptidyl-prolyl cis-trans isomerase activity, as was previously reported for AtCYP19-4. Rice plants overexpressing OsCYP19-4 showed cold-resistance phenotypes with significantly increased tiller and spike numbers, and consequently enhanced grain weight, compared with wild-type plants. Based on these results, the authors suggest that OsCYP19-4 is required for developmental acclimation to environmental stresses, especially cold. Furthermore, the results point to the potential of manipulating OsCYP19-4 expression to enhance cold tolerance or to increase biomass.
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