Review
Cell Biology
Ana Bura, Sara Cabrijan, Iris Duric, Tea Bruketa, Antonija Jurak Begonja
Summary: Phosphoinositides (PIs) are phosphorylated lipids that have diverse cellular functions including regulation of trafficking, actin reorganization, and cell signaling. The two most abundant PIs in cells are phosphatidylinositol4-monophosphate (PI4P) and phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2], which localize to different cellular compartments and regulate various processes. The levels of PIs are controlled by multiple kinases and phosphatases, and understanding their localization and function is important for studying cellular processes.
Article
Biochemistry & Molecular Biology
Hannes Maib, David H. Murray
Summary: The study reveals the molecular mechanism of exocyst-mediated membrane tethering, showing that phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) plays a crucial role in this process. The researchers demonstrate that vesicles containing phosphatidylinositol 4-phosphate (PI(4)P) are rapidly converted to PI(4,5)P-2 through the recruitment of the lipid kinase PIP5K1C, which drives the recruitment of exocyst complex and membrane tethering.
Review
Plant Sciences
Jingjing Xing, Liang Zhang, Zhikun Duan, Jinxing Lin
Summary: The article provides a summary of defense-associated signal transduction processes in plants, focusing on the connection between phospholipid-based signaling and membrane trafficking. It discusses the role of lipid diversity in these processes and highlights the mechanisms that regulate plant immunity by mediating feedback between phospholipid-based signaling and membrane trafficking.
TRENDS IN PLANT SCIENCE
(2021)
Article
Biology
Ana Bura, Antonija Jurak Begonja
Summary: Phosphoinositides play crucial roles in cells with limited understanding of their localization and functions in platelets. This study investigated the localization of PI(4,5)P-2 and PI4P in resting and activated platelets through antibody staining. The intracellular pools of PI(4,5)P-2 and PI4P in platelets can be modulated by inhibitors of OCRL phosphatase and PI4KIII alpha kinase, with a more sensitive response in activated platelets.
Article
Biochemistry & Molecular Biology
Fumi Hoshino, Fumio Sakane
Summary: AP180 protein is crucial for driving endocytosis by interacting with clathrin and it can be selectively regulated by 18:0/22:6-PA, which attenuates its interaction with clathrin. This novel mechanism sheds light on the disassembly of AP180/clathrin-containing cages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Yi Wen, Volker M. Vogt, Gerald W. Feigenson
Summary: Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) is a crucial component located at the inner leaflet of the cell membrane, constituting only 1-2% of total membrane lipids. Its synthesis and turnover are spatially and temporally regulated. Various factors, such as multivalent cations and cellular proteins interacting with PI(4,5)P-2, contribute to its versatile and dynamic distribution within membranes.
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 90, 2021
(2021)
Article
Nanoscience & Nanotechnology
Jiapei Yang, Li Xu, Ling Di, Yue Su, Xinyuan Zhu
Summary: PEG, as the gold standard for stealth polymer materials, is widely used in drug delivery due to its excellent properties. However, the lack of understanding about the fate of PEG at the cellular level has hindered its application in diagnosis and therapy. Research showed that PEG could be internalized into cells in 1 hour, localized to lysosomes, cytosol, ER, and mitochondria. The fate of PEG in cells could be regulated by conjugating different small molecules, which is crucial for the rational design of PEGylation for drug delivery.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Plant Sciences
Mariko Kato, Machiko Watari, Tomohiko Tsuge, Sheng Zhong, Hongya Gu, Li-Jia Qu, Takashi Fujiwara, Takashi Aoyama
Summary: Phosphatidylinositol 4-phosphate 5-kinase (PIP5K) and its product PtdIns(4,5)P-2 play a crucial role in pollen germination, possibly by establishing germination polarity in pollen.
Article
Plant Sciences
Femke de Jong, Teun Munnik
Summary: Membranes are crucial for cell and organelle functions, providing electrochemical energy, creating compartmentalized microenvironments, and facilitating molecule transport and cell signaling. Advances in protein crystallography, lipid-binding studies, and subcellular localization analyses have greatly enhanced our understanding of membrane functions and signaling pathways.
Review
Plant Sciences
Xiaobo Zhu, Junjie Yin, Hongming Guo, Yuping Wang, Bingtian Ma
Summary: The vesicle trafficking apparatus is crucial for maintaining the homeostasis of membrane-enclosed organelles in eukaryotic cells. Research on vesicle trafficking in plants, particularly rice, is limited, and further investigations are needed to uncover the mysteries of this system.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Joseph S. Erlichman, James C. Leiter
Summary: Metal oxide nanoparticles have potential as theranostic agents, but the complexity of their chemistry as they interact with different environments and proteins may hinder their clinical translation. Inadequate translational models and discrepancies between simplified experiments and biological activities in organisms pose challenges for accurately predicting the therapeutic potential of metal oxide NPs. Understanding the evolving pharmacology of these NPs in biological systems is crucial for establishing effective translational test systems.
Review
Cell Biology
Anna Marie Sokac, Natalie Biel, Stefano De Renzis
Summary: This review discusses how changes in cell shapes during morphogenesis are related to the remodeling of the plasma membrane and actin cytoskeleton. The cellularization of the Drosophila embryo is highlighted as a model system to uncover the principles of how membrane and actin dynamics are co-regulated in both space and time to drive morphogenesis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Computer Science, Interdisciplinary Applications
Tiankui Zhang, Charles W. Wolgemuth
Summary: The dynamics of thin, membrane-like structures are crucial in cell biology, but simulating large scale deformations remains challenging due to spatial heterogeneity. A general computational framework has been developed to simulate the dynamics of membranes and vesicles, successfully predicting equilibrium shapes of multiphase vesicles.
JOURNAL OF COMPUTATIONAL PHYSICS
(2022)
Article
Plant Sciences
Fan Wang, Zhijun Cheng, Jiachang Wang, Feng Zhang, Baocai Zhang, Sheng Luo, Cailin Lei, Tian Pan, Yongfei Wang, Yun Zhu, Min Wang, Weiwei Chen, Qibing Lin, Shanshan Zhu, Yihua Zhou, Zhichao Zhao, Jie Wang, Xiuping Guo, Xin Zhang, Ling Jiang, Yiqun Bao, Yulong Ren, Jianmin Wan
Summary: This study identified a rice mutant, dph1-1, with limited cell expansion, resulting in reduced plant height and smaller panicles and grains. The protein OsSCD2, homologous to Arabidopsis SCD2, was found to be associated with clathrin and involved in defective endocytosis and post-Golgi trafficking in the dph1-1 mutant. The mutant also displayed impaired cellulose synthesis. These findings suggest that OsSCD2 plays a crucial role in clathrin-related vesicular trafficking and is important for maintaining plant growth in rice.
Article
Cell Biology
Martin Sztacho, Barbora Salovska, Jakub Cervenka, Can Balaban, Peter Hoboth, Pavel Hozak
Summary: Analysis of nuclear protein interactions with phosphoinositides reveals the role of PIP2 in regulating gene expression, RNA splicing, and cell cycle processes. The identified proteins are involved in various functions within the nucleus, with different PIP2-binding motifs influencing their localization and activity. This study provides insights into the molecular mechanism of nuclear PIP2 protein interaction and offers a methodology for further research on PIPs and other protein ligands.
Article
Plant Sciences
Jitka Ortmannova, Juraj Sekeres, Ivan Kulich, Jiri Santrucek, Petre Dobrev, Viktor Zarsky, Tamara Pecenkova
Summary: The exocyst complex with the EXO70B2 subunit plays a specific role in papilla and encasement formation, working in coordination with the SYP121 SNARE complex in defense against non-adapted fungal pathogens. The interaction between EXO70B2 and SYP121 proteins contributes to the timely formation of papillae and haustorial encasements, enhancing the plant's resistance to fungal invasion. The findings highlight the cooperation between the exocyst and a SNARE protein in defending against non-adapted pathogens.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Tamara Pecenkova, Premysl Pejchar, Tomas Moravec, Matej Drs, Samuel Haluska, Jiri Santrucek, Andrea Potocka, Viktor Zarsky, Martin Potocky
Summary: PR1 protein, commonly used as a marker for biotic stress monitoring in plants, undergoes partial proteolytic processing en route from the endoplasmic reticulum, with only nonmutated variants or processing-mimicking ones being secreted to the apoplast. The released protein fragment acts as a modulator of plant defense responses, with tissue-localized and host plant ontogenesis-dependent immunity potential.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Patricia Scholz, Premysl Pejchar, Max Fernkorn, Eliska Skrabalkova, Roman Pleskot, Katharina Blersch, Teun Munnik, Martin Potocky, Till Ischebeck
Summary: Pollen tube growth requires tight regulation of pectin secretion, and molecules such as phosphoinositides and phosphatidic acid play important roles in this process. A study found that DGK5, a kinase, is localized in the plasma membrane and is involved in the regulation of pectin secretion and lipid signaling, contributing to polar tip growth in pollen tubes.
Article
Plant Sciences
Jakub Zarsky, Vojtech Zarsky, Martin Hanacek, Viktor Zarsky
Summary: The terrestrial habitats during the Cryogenian period were possibly covered by global snow and ice. Recent studies suggest that multicellular charophytes evolved in response to Cryogenian cooling. This period also saw the likely origin of the common ancestor of Zygnematophyceae and Embryophyta. The expansion of terrestrial flora during the Marinoan glaciation was represented not only by Chlorophyta but also by Streptophyta and Zygnematophyceae.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Hannah Elisa Krawczyk, Siqi Sun, Nathan M. Doner, Qiqi Yan, Magdiel Sheng Satha Lim, Patricia Scholz, Philipp William Niemeyer, Kerstin Schmitt, Oliver Valerius, Roman Pleskot, Stefan Hillmer, Gerhard H. Braus, Marcel Wiermer, Robert T. Mullen, Till Ischebeck
Summary: This study identifies three proteins in Arabidopsis thaliana that form a complex tethering a subset of lipid droplets to the plasma membrane in seedlings.
Article
Plant Sciences
Klara Batystova, Lukas Synek, Martina Klejchova, Edita Jankova Drdova, Peter Sabol, Martin Potocky, Viktor Zarsky, Michal Hala
Summary: The exocyst complex is involved in the regulation of polarized secretion in eukaryotic cells. In seed plants, there are two isoforms of the SEC15 gene, with SEC15a mainly functioning in pollen and SEC15b mainly functioning in sporophyte. Loss of SEC15a leads to impaired pollen tube, while loss of SEC15b results in stunted sporophyte development.
Article
Plant Sciences
Tetiana Kalachova, Eliska Skrabalkova, Stephanie Pateyron, Ludivine Soubigou-Taconnat, Nabila Djafi, Sylvie Collin, Juraj Sekeres, Lenka Burketova, Martin Potocky, Premysl Pejchar, Eric Ruelland
Summary: Activation of Arabidopsis DIACYLGLYCEROL KINASE 5 is necessary for an appropriate immune response during plant-microbe recognition. The perception of flagellin, a key protein in pattern-triggered immunity in plants, initiates a signaling cascade involving protein phosphorylation and reactive oxygen species production.
Article
Plant Sciences
Lucas Alves Neubus Claus, Derui Liu, Ulrich Hohmann, Nemanja Vukasinovic, Roman Pleskot, Jing Liu, Alexei Schiffner, Yvon Jaillais, Guang Wu, Sebastian Wolf, Daniel Van Damme, Michael Hothorn, Eugenia Russinova
Summary: A BR binding-deficient Arabidopsis mutant was designed to re-examine the BRI1 endocytosis and signal attenuation model. The study showed that despite impaired phosphorylation and ubiquitination, the BR binding-deficient BRI1 can still internalize, indicating that BRI1 internalization relies on different endocytic machineries. Furthermore, the mutant provides opportunities to study non-canonical ligand-independent BRI1 functions.
Article
Plant Sciences
David Usak, Samuel Haluska, Roman Pleskot
Summary: This article reviews recent studies on the role of callose and specific callose synthases (CalSs) in cell wall remodeling, with a particular focus on cytokinesis. A robust phylogenetic analysis of CalS across the plant kingdom suggests a 3-subfamily distribution of CalS. The possible linkage between the evolution of CalSs and their function in specific cell types and processes is also discussed.
Article
Plant Sciences
Ankush Ashok Saddhe, Martin Potocky
Summary: Plant secretory phospholipase A(2) (sPLA(2)) is a family of lipolytic enzymes involved in plant physiological functions. This study provides a comparative analysis of PA2c domain-containing genes across plants and reveals the widespread occurrence of multiple sPLA(2) in most land plants. A novel PA2c-containing gene family, termed PLA(2)-like, is also identified. Further analysis shows clade-specific gene expressions and conserved features within the PA2c domain for the three clades. This study contributes to understanding the evolutionary significance of the PA2c family and lays the foundation for future research on sPLA(2) and PLA(2)-like characterization in plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jie Wang, Qihang Jiang, Roman Pleskot, Peter Grones, Elmehdi Bahafid, Gregoire Denay, Carlos Galvan-Ampudia, Xiangyu Xu, Michael Vandorpe, Evelien Mylle, Ive De Smet, Teva Vernoux, Ruediger Simon, Moritz K. Nowack, Daniel Van Damme
Summary: Endocytosis is important for plant response to stimuli and TPC plays an essential role in endocytosis in Arabidopsis plants. Knockout or knockdown of TPC subunits cause severe phenotypes, but partially functional alleles cause mild deviations. Reduced TPC-dependent endocytosis leads to hypersensitivity to CLAVATA3 peptide signaling.
Article
Biochemistry & Molecular Biology
Tamara Pecenkova, Martin Potocky
Summary: Small secreted proteins in plants, such as PR1 and CLE, play important roles in development and environmental responses. However, their localization, trafficking, and exocytosis details are poorly understood. In this study, we investigated the secretion modes by exploring the co-expression links between secreted cargoes and regulators using computational approaches. Our results suggest that there might be specific requirements for the synthesis and secretion of PR1 and CLE proteins, which need to be further investigated experimentally.
PLANT SIGNALING & BEHAVIOR
(2023)
Correction
Plant Sciences
Peter Grones, Andreas De Meyer, Roman Pleskot, Evelien Mylle, Michael Kraus, Michael Vandorpe, Klaas Yperman, Dominique Eeckhout, Jonathan Michael Dragwidge, Qihang Jiang, Jonah Nolf, Benjamin Pavie, Geert De Jaeger, Bert De Rybel, Daniel Van Damme
