Journal
CARDIOVASCULAR RESEARCH
Volume 102, Issue 1, Pages 166-175Publisher
OXFORD UNIV PRESS
DOI: 10.1093/cvr/cvu008
Keywords
Arrhythmia; Ankyrin; Spectrin; TREK-1; Two-pore potassium channel
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Funding
- National Institutes of Health (NIH) [HL 096805, HL114893, HL084583, HL083422, HL114383, HL079031, HL096652, HL113001, HL070250]
- James S. McDonnell Foundation
- Saving Tiny Hearts Society
- American Heart Association
- Fondation Leducq Transatlantic Alliance for CaMKII Signaling
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Aims Cardiac function depends on the highly regulated and co-ordinate activity of a large ensemble of potassium channels that control myocyte repolarization. While voltage-gated K+ channels have been well characterized in the heart, much less is known about regulation and/or targeting of two-pore K+ channel (K-2P) family members, despite their potential importance in modulation of heart function. Methods and results Here, we report a novel molecular pathway for membrane targeting of TREK-1, a mechano-sensitive K-2P channel regulated by environmental and physical factors including membrane stretch, pH, and polyunsaturated fatty acids (e. g. arachidonic acid). We demonstrate that beta(IV)-spectrin, an actin-associated protein, is co-localized with TREK-1 at the myocyte intercalated disc, associates with TREK-1 in the heart, and is required for TREK-1 membrane targeting. Mice expressing bIV-spectrin lacking TREK-1 binding (qv(4J)) display aberrant TREK-1 membrane localization, decreased TREK-1 activity, delayed action potential repolarization, and arrhythmia without apparent defects in localization/function of other cardiac potassium channel subunits. Finally, we report abnormal bIV-spectrin levels in human heart failure. Conclusions These data provide new insight into membrane targeting of TREK-1 in the heart and establish a broader role for beta(IV)-spectrin in organizing functional membrane domains critical for normal heart function.
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